Identification of Rhizoctonia solani AG 1-IB in Lettuce, AG 4 HG-I in Tomato and Melon, and AG 4 HG-III in Broccoli and Spinach, in Brazil     

Eiko E. Kuramae  Alexandre L. Buzeto  Maisa B. Ciampi  Nilton L. Souza 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(4):391-395

Fungi isolated in Brazil, from lettuce, broccoli, spinach, melon and tomato, were identified as Rhizoctonia solani. All lettuce isolates anastomosed with both AG 1-IA and IB subgroups and all isolates from broccoli, spinach, melon and tomato anastomosed with AG 4 subgroup HG-I, as well as with subgroups HG-II and HG-III. DNA sequence analyses of ribosomal internal transcribed spacers showed that isolates from lettuce were AG 1-IB, isolates from tomato and melon were AG 4 HG-I, and isolates from broccoli and spinach were AG 4 HG-III. The tomato isolates caused stem rot symptoms, the spinach, broccoli and melon isolates caused hypocotyl and root rot symptoms on the respective host plants and the lettuce isolates caused bottom rot. This is the first report on the occurrence in Brazil of R. solani AG 4 HG-I in tomato and melon, of AG 4 HG-III in broccoli and spinach and of AG 1-IB in lettuce.  相似文献   

Evaluation of cross-protection with an attenuated isolate of Bean yellow mosaic virus by differential detection of virus isolates using RT-PCR     

Eiko Nakazono-Nagaoka  Chikako Sato  Yoshitaka Kosaka  Tomohide Natsuaki 《Journal of General Plant Pathology》2004,70(6):359-362

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RT-PCR-RFLP analysis for evaluating cross protection by an attenuated isolate of Cucumber mosaic virus     

Eiko Nakazono-Nagaoka  Masako Suzuki  Yoshitaka Kosaka  Tomohide Natsuaki 《Journal of General Plant Pathology》2005,71(3):243-246

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Response of growth agglutinating antibody and protection of pigs inoculated with swine erysipelas live vaccine   总被引：3，自引：0，他引：3  

T Sawada  M Muramatsu  K Seto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1979,41(6):593-600

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Development of a PCR test for the identification of Staphylococcus intermedius based on the 16S rDNA sequence     

Wakita Y  Kawano J  Shimizu A  Hajek V  Tomisaka E  Yasuda R  Matsuo E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(7):603-605

The development of a PCR assay based on the 16S ribosomal RNA gene (rDNA) sequence was carried out for the identification of Staphylococcus intermedius. Sixty-six strains of S. intermedius, 70 of Staphylococcus aureus and 2 of Staphylococcus hyicus were examined for the assay. The 16S rDNA, of which the PCR target fragment makes up 901 bp corresponding to the sequence data of the gene, was detected in all strains of S. intermedius, but it was not detected in any strains of either S. aureus or S. hyicus. These results suggest that the PCR allows a simple and precise identification of S. intermedius.  相似文献   

Distribution of mecA-harboring staphylococci in healthy mares     

Yasuda R  Kawano J  Matsuo E  Masuda T  Shimizu A  Anzai T  Hashikura S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(9):821-827

The prevalence of staphylococci that harbor the mecA gene responsible for methicillin resistance was examined in healthy breeding mares. Staphylococci often cause diseases of horses such as metritis, keratitis, and abscess. Methicillin-resistant staphylococci would make antibiotic treatments ineffective, so it may be significant to know the distribution of mecA-harboring staphylococci in mares. Isolation of mecA-harboring staphylococci was achieved from nares and pasterns of 100 mares in Hokkaido, Japan. From 13% of the mares, mecA-harboring staphylococci, including 15 isolates of Staphylococcus sciuri and 3 of Staphylococcus lentus, were isolated. Isolates of S. sciuri were found to be genetically polyclonal by pulsed-field gel electrophoresis. These isolates produced no PCase and showed low or no resistance to beta-lactam and other classes of antibiotics. Distribution of staphylococcal species and levels of antibiotic resistance were found to be different between isolates from the present mares and those previously reported from riding-horses. Antibiotic pressure may lead to these differences. In addition, it appears that mecA-harboring S. sciuri may be native to horses.  相似文献   

Infection of bovine immunodeficiency virus and bovine leukemia virus in water buffalo and cattle populations in Pakistan     

Meas S  Seto J  Sugimoto C  Bakhsh M  Riaz M  Sato T  Naeem K  Ohashi K  Onuma M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(3):329-331

A survey of antibodies to bovine immunodeficiency virus (BIV) known as bovine lentivirus and bovine leukemia virus (BLV) was conducted with samples from water buffalo and cattle populations in Pakistan. A total of 370 water buffaloes and 76 cattle were tested, and 10.3% and 15.8%, respectively, were found positive for anti-BIV p26 antibodies determined by Western blotting, while 0.8% of water buffaloes and no cattle were positive for anti-BLV antibodies determined by immunodiffusion test. BIV-seropositive water buffaloes and cattle were found to have BIV proviral DNA in the peripheral blood mononuclear cells determined by nested polymerase chain reaction. This is the first report of BIV infections in water buffaloes.  相似文献   

Oxygen isotopic compositions of asteroidal materials returned from Itokawa by the Hayabusa mission     

Yurimoto H  Abe K  Abe M  Ebihara M  Fujimura A  Hashiguchi M  Hashizume K  Ireland TR  Itoh S  Katayama J  Kato C  Kawaguchi J  Kawasaki N  Kitajima F  Kobayashi S  Meike T  Mukai T  Nagao K  Nakamura T  Naraoka H  Noguchi T  Okazaki R  Park C  Sakamoto N  Seto Y  Takei M  Tsuchiyama A  Uesugi M  Wakaki S  Yada T  Yamamoto K  Yoshikawa M  Zolensky ME 《Science (New York, N.Y.)》2011,333(6046):1116-1119

Meteorite studies suggest that each solar system object has a unique oxygen isotopic composition. Chondrites, the most primitive of meteorites, have been believed to be derived from asteroids, but oxygen isotopic compositions of asteroids themselves have not been established. We measured, using secondary ion mass spectrometry, oxygen isotopic compositions of rock particles from asteroid 25143 Itokawa returned by the Hayabusa spacecraft. Compositions of the particles are depleted in (16)O relative to terrestrial materials and indicate that Itokawa, an S-type asteroid, is one of the sources of the LL or L group of equilibrated ordinary chondrites. This is a direct oxygen-isotope link between chondrites and their parent asteroid.  相似文献   

Five Years of Continuous Thaumetopoea processionea Monitoring: Tracing Population Dynamics in an Arable Landscape of South-Western Germany     

Eiko Wagenhoff  Holger Veit 《Gesunde Pflanzen》2011,63(2):51-61

Within this paper we summarize the results of the Thaumetopoea processionea monitoring at two observation sites and try to interpret population dynamics from 2006 to 2010 considering meteorological data. Furthermore, observations on the behaviour of neonates associated with oak budburst are presented and discussed. In 2007 large larval colonies occurred and population densities peaked. Massive defoliation and larval migration due to food shortage were observed regularly leading to reduced female moth fecundity. Unfavourable weather conditions probably abetted OPM population decline in spring 2008. Additionally oak budburst was delayed remarkably. More detailed observations on the hatching process and the behaviour of single colonies revealed increasing mortality after two and a half weeks of starvation. The 2009 results indicated another increase of population densities, but exceeded cool and humid weather conditions in May 2010 presumably prevented a new OPM outbreak. In accordance with previous studies egg parasitisation seems to play a minor role in OPM population dynamics.  相似文献   

Precipitation chemistry in Japan 1989–1993     

Hiroshi Hara  Moritsugu Kitamura  Atsuko Mori  Izumi Noguchi  Tsuyoshi Ohizumi  Sinya Seto  Tadashi Takeuchi  Teruyuki Deguchi 《Water, air, and soil pollution》1995,85(4):2307-2312

Precipitation chemistry in Japan was discussed on a wet-only sample database obtained in a nationwide survey from April 1989 to March 1993. Wet-only samples were collected at 29 stations over Japan on a biweekly basis. Commonly determined chemical parameters were measured in laboratories. The volume-weighted annual mean pH at each site ranged from 4.50 to 5.83 with a mean of 4.76. Concentration ranges and means (parenthesized) on an equivalent basis for major ions were as follows: nss-SO₄ ^2–; 5.2–58.9 (38.6), NO₃ ^–; 1.8–25.0 (14.1), NH₄ ⁺; 0.55–29.8 (18.3), nss-Ca²⁺; 2.0–34.5(14.2), Na⁺; 6.4–275.3 (49.1), Cl^–; 13.7–322.4 (63.5) eq L^–1. Acid-base relationships for Phase-II records were quantitatively discussed in terms of three measures: pH, fractional acidity, and our proposed pA_i.  相似文献