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Belloy L Giacometti M Abdo EM Nicolet J Krawinkler M Janovsky M Bruderer U Frey J 《Veterinary research》2001,32(2):155-164
The serological cross reactions between Mycoplasma conjunctivae, the etiological agent of infectious keratoconjunctivitis (IKC), and the antigenetically and phylogenetically closely related Mycoplasma ovipneumoniae, which is often found in sheep, were analysed. Cross reacting antigens were identified using sera from sheep with IKC and from sheep of herds known to be free of IKC, as well as rabbit hyperimmune serum specific to the two Mycoplasma species. Cross reactions were predominantly due to the strongly antigenic proteins of 42 kDa and 83 kDa. Serospecific antigens of M. conjunctivae could be separated from cross-reacting antigens by the extraction of Tween 20-soluble membrane proteins. The Tween 20-extracted proteins of the M. conjunctivae strain HRC/581T were used for the development of an indirect ELISA test. This ELISA test was shown to be a useful serological method for the diagnosis of M. conjunctivae infections and to identify infected sheep herds. 相似文献
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M. Abdo Y. Z. Hosaka A. Erasha M. Nada S. Ali M. Uehara 《Anatomia, histologia, embryologia》2014,43(4):257-264
Studies of ocular development in the dromedary camel (Camelus dromedarius) have not been reported previously. The aim of the present investigation was therefore to document the major landmarks and the time course in the prenatal development of the eye tunics in dromedary camel and its accommodation with the surrounding hard environment of the desert. Serial histological sections of dromedary camel embryos and foetuses were used. Age estimation was made on the basis of gestational size, crown vertebral‐rump length (CVRL), which ranged 1.2–110 cm. The eye of the dromedary camel developed in a similar manner to that of the human and domestic animals eyes; the principal differences were in the time of occurrence of certain developmental events, pigmented peripheral cornea near the limbus, a remarkably thickened Descemet's membrane and pigmentation in the corneo‐scleral junction, which represent an adaptive modification in relation to a severe environment. 相似文献
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Elgioushy Magdy Rizk Mohamed Abdo El-Adl Mohamed Elhadidy Mohamed El-Khodery Sabry 《Tropical animal health and production》2019,51(4):847-852
Tropical Animal Health and Production - Panting syndrome and respiratory infection have been recorded in complicated cases of foot and mouth disease (FMD) in cattle. However, investigations on the... 相似文献
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Sabreen E. Fadl Ghada A. El‐Gammal Walied S. Abdo Mohamed Barakat Osama A. Sakr Eldsokey Nassef Doaa M. Gad Hamdy S. El‐Sheshtawy 《Aquaculture Research》2020,51(3):1120-1132
The present investigation aimed to evaluate the effect of dietary chitosan supplementation on growth performance, body composition, immune response and histopathology of Nile tilapia, and also the in vitro antibacterial activity of chitosan against Streptococcus agalactiae (S. agalactiae). About 180 fish (average body weight 39.3 ± 0.3 g) were randomly divided into three groups according to chitosan supplementation: control group (basal diet without chitosan), Ch3 group (3 g chitosan/kg diet) and Ch5 group (5 g chitosan/kg diet). Growth performance parameters and body proximate composition were measured before infection but biochemical parameters and lysozyme and antibacterial activities before and after experimental infection. Results of the present investigation showed dietary chitosan (5 g chitosan/kg diet) significantly (p < .05) improved growth performance parameters, body composition (dry matter, crude protein, ether extract, ash, and carbohydrate) and serum biochemistry (total protein, albumin, globulin, with no effect on AST, ALT, urea and creatinine) before infection in Ch5 group than the control. After infection, liver enzymes (serum AST and ALT) were maintained lower in fish fed Ch3 or Ch5 than the control. Serum lysozyme and bactericidal activities significantly increased (p < .05) in chitosan groups before and after the challenge. The mortality rate was markedly reduced in the Ch3 group and prohibited in the Ch5 group after the experimental infection. In conclusion, feeding 3 or 5 g chitosan/kg diet increased the growth rate and improved FCR of Nile tilapia. In addition, it reduced mortality by its antibacterial and immunostimulant effects. 相似文献
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Nuñez-Vazquez EJ Garcia-Ortega A Campa-Cordova AI Abdo de la Parra I Ibarra-Martinez L Heredia-Tapia A Ochoa JL 《Marine drugs》2012,10(2):329-339
The toxin content in various life cycle stages of tank-cultivated bullseye puffer (Sphoeroides annulatus) were analyzed by mouse bioassay and ESI-MS spectrometry analysis. The presence of toxin content was determined in extracts of sperm, eggs, embryo, larvae, post-larvae, juvenile, pre-adult, and adult fish, as well as in food items used during the cultivation of the species. Our findings show that only the muscle of juveniles, the viscera of pre-adults, and muscle, liver, and gonad of adult specimens were slightly toxic (<1 mouse unit). Thus, cultivated S. annulatus, as occurs with other cultivated puffer fish species, does not represent a food safety risk to consumers. This is the first report of toxin analysis covering the complete life stages of a puffer fish under controlled conditions. 相似文献
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J. E. Stewart Z. Abdo R. K. Dumroese N. B. Klopfenstein M.‐S. Kim 《Forest Pathology》2012,42(3):220-228
Fusarium species can cause damping‐off and root rot of young conifer seedlings, resulting in severe crop and economic losses in forest nurseries. Disease control within tree nurseries is difficult because of the inability to characterize and quantify Fusarium spp. populations with regard to disease potential because of high variability in isolate virulence. Fusarium isolates were collected from healthy and diseased seedlings of Douglas‐fir (Pseudotsuga menziesii) and western white pine (Pinus monticola) from a nursery in Idaho, USA. Molecular markers such as DNA sequences (mitochondrial small subunit and nuclear translation elongation factor 1‐alpha) and amplified fragment length polymorphism were used to identify isolates as either F. oxysporum or F. commune. In addition, diagnostic primers were developed to detect and distinguish F. commune from F. oxysporum. In vitro and greenhouse virulence tests were completed on Douglas‐fir germinants and seedlings. For Douglas‐fir germinants and seedlings, F. oxysporum isolates generally caused less severe symptoms, whereas most F. commune isolates caused mortality through damping‐off. This is the first report of direct evidence that F. commune can cause damping‐off disease on Douglas‐fir seedlings under greenhouse conditions. 相似文献
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Crisantema Hernández Jazmín Sarmiento-Pardo Blanca González-Rodríguez & Isabel Abdo Parra de la 《Aquaculture Research》2004,35(12):1153-1157
A feeding trial was conducted to evaluate the potential of replacing fish meal with co‐extruded wet tuna viscera and corn meal in diets for juvenile white shrimp Litopenaeus vannamei. Five practical diets were formulated to contain 30% crude protein and 16.6 kJ g?1. The feeding experiment was carried out in a recirculating seawater system with fifteen 25‐L tanks with 15 shrimp per tank. Each dietary treatment was fed in triplicate in a completely randomized design for 41 days. Percent weight gain (WG%), survival (S%) and feed conversion ratio (FCR) were calculated, comparisons of these parameters showed no statistical differences (P>0.05) among treatments. The shrimp almost tripled their weight during the feeding trial. Under the experimental conditions S%, WG% and FCR were either improved or were not significantly influenced by the replacement of fish meal with double co‐extruded viscera+corn, except for a slight increase in feed consumption without affecting FCR. Hence, this product can be included by up to 40% in practical shrimp diets without any detrimental effects. 相似文献
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Combined effects of dietary malic acid and Bacillus subtilis on growth,gut microbiota and blood parameters of Nile tilapia (Oreochromis niloticus) 
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下载免费PDF全文 The study investigated effect of dietary supplementation with malic acid, Bacillus subtilis and a mixture of the two (3 × 2 factorial trial) on Nile tilapia (Oreochromis niloticus) health. Treatment groups (T1–T6) were fed diets containing three levels of malic acid (0.0, 5.0 and 10.0 g/kg), each of which was supplemented with 0 and 1.1 × 105 cfu/g B. subtilis, respectively. Each treatment group was assigned randomly to triplicate groups of 35 fish (5.26 ± 0.06 g) in 18 tanks for 84 days. The results indicated that survival was higher in all dietary treatments as compared to the control fed fish. The highest values of FBW, WG and SGR (%), PER, PPV and ER were recorded in groups T4 and T6, which were fed diets supplemented with 5 g malic acid/kg and 1.1 × 105 cfu/g B. subtilis and 10 g malic acid/kg and 1.1 × 105 cfu/g B. subtilis, respectively. The best value of FCR was obtained in groups T4 and T6. The lowest total bacterial count in the gut and faeces was detected in fish from group T6. The values of haematocrit, haemoglobin, red blood cells, white blood cells, total protein, albumin and globulin were significantly higher (p < .05) in fish from groups T4 and T6 (diets supplemented with malic acid and B. subtilis). As compared to the control fed fish, te mixture of these substances are promising as immune enhancher in aquacultured fish. 相似文献