排序方式： 共有4条查询结果，搜索用时 0 毫秒
1
1.
Khaled Mohamed El-Dakhly El-Shaymaa El-Nahass Kosei Inui Junpei Kimura Hiroki Sakai Tokuma Yanai 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(4):421-424
An adult male rufous turtle dove, Streptopelia (S.) orientalis (Aves: Columbiformes), was found dead in Yorii-machi Town, Osato District 369-1217, Saitama Prefecture, Japan, and subjected to necropsy. A large number of immobile hypopi (deutonymphs) of the hypoderatid mite, Hypodectes (H.) propus (Acarina: Hypoderatidae), were found individually encapsulated subcutaneously primarily in the adipose tissue. The mites were 1.43 mm in length and 0.44 mm in width on average, and had provoked mild inflammatory reactions that predominantly manifested as foamy macrophages and lymphoplasmocytes. PCR analysis using ribosomal DNA extracted from paraffin-blocked tissues produced a 240 bp band specific for hypoderatids. Based on the morphological features (distinct coxal apodemes, especially in the anterior portion) and PCR-based findings, the hypopi were identified as H. propus. To the best of our knowledge, this is the first case describing the subcutaneous mite H. propus in a rufous turtle dove, S. orientalis, in Japan. This study also highlights the use of paraffin blocks as a source of tissue DNA for molecular evaluation. 相似文献
2.
Contents: Blood samples were collected from 50 normal male and female goats belonging to five Egyptian breeds, and also from 22 sterile females. Leukocyte cultures were set up and chromosomal preparations were made. The karyotype of the goats examined was identical with that reported in the literature for foreign breeds, consisting of 58 acrocentric autosomes, a large, acro centric X and a small, metacentric Y chromosome. All acrocentric chromosomes had well defined C-bands, while the Y chromosome was uniformly dark. Chromosomal aberrations were infrequent in the normal animals, except in one male which had a high rate of polyploidy. There were no significant differences in cytological aberrations between breeds and sexes, except for polyploidy, which was caused by the one aberrant male with high polyploidy. The 22 sterile females were composed of 14 individuals which displayed XX/XY mosaicism and eight animals with only XX leukocytes. Among the 14 mosaics, 10 animals were intersexes, one displayed blind halves of the udder, and three were morphologically normal females. The eight sterile XX females were morphologically normal. Chromosomal aberrations were higher in sterile females than in fertile females. Sterile XX females had more aberrations than XX/XY mosaics. The aberrations consisted of chromatid breaks, centric fusion, chromosome and chromatid gaps and peridiploidy, in that order of significance. Concluding, the present study adds evidence for a link between sterility and chromosomal aberration. The high incidence of intersexuality associated with XX/XY mosaicism in polled goats warrants further study. Inhalt: ZTtogenetische Studien an fünf ägyptischen Ziegenrassen Von 50 normalen Ziegen fünf ägyptischer Rassen und von 22 unfruchtbaren Tieren wurden Blutproben gewonnen und Leukozytenkulturen und Chromosompräparationen angefertigt. Der untersuchte Karyotyp der Ziegen entsprach dem in der Literatur von ausländischen Rassen berichteten und besteht aus 58 akrozentrischen Autosomen, einem groβen akrozentrischen X- und einem kleinen metazentrischen Y-Chromosom. Alle akroxentrischen Chromosomen haben klar definierte C-Bänderung, während das Y-Chromosom einheitlich dunkel ist. Chromosomale Abweichungen sind in normalen Tieren selten, nur ein Bock hatte eine hohe Polyploidierate. Signifikante Unterschiede in zytologischen Abweichungen konnten zwischen Rassen und Geschlechtem nicht festgestellt werden, mit Ausnahme der Polyploidie des genannten Bockes. Unter 22 sterilen Ziegen waren 14 mit XX/XY-Mosaik und 8 XX-Tiere. Unter den 14 Mosaiken waren 10 Intersexe, ein Tier hatte eine blinde Euterhälfte und drei waren morphologisch normal. Die 8 unfruchtbaren XX-Ziegen waren morphologisch normal. Chromosomale Aberrationen waren in sterilen Ziegen höher als in fruchtbaren Tieren, die sterilen XX-Ziegen hatten mehr Abweichungen als die Ziegen mit XX/XY-Mosaiken. Die Aberrationen bestanden in Chromatidbrüchen, zentrischen Fusionen, Chromosom- und Chromatidlücken und Peridiploidie, und zwar in dieser Reihenfolge. Zusammenfassend kann festgestellt werden, daß die gegenwärtigen Untersuchungen Hinweise für eine Verbindung zwischen Sterilität und chromosomalen Abweichungen liefert. Die gesteigerte Häufigkeit der Intersexualität in Verbindung mit XX/XY-Mosaiken in hornlosen Ziegen macht weitere Untersuchungen wünschenswert. 相似文献
3.
El-Dakhly KM El-Nesr KA El-Nahass el-S Hirata A Sakai H Yanai T 《Tropical animal health and production》2011,43(8):1549-1554
This study was performed for the purpose of investigating the prevalence of Sarcocystis spp. in buffaloes in Beni-Suef Governorate, Egypt. Both macroscopic (Sarcocystis fusiformis) and microscopic (Sarcocystis levinei) cysts were recognized, and were differentiated by their morphological features and location in the tissues. Of 379 buffaloes
examined in abattoirs in Beni-Suef, 299 were found to be infected, with an overall prevalence of 78.9%. Depending on age,
three categorized groups of naturally infected buffaloes were examined: male buffalo calves aged 1.5–2 years, adult females
aged 2–5 years, and females older than 5 years. Among these groups, infection rates were 74.5%, 82.3%, and 81.2%, respectively.
Organs examined included esophagus, tongue, and heart. Macroscopic cysts were examined by the naked eye through meat inspection
in abattoirs, while the pepsin-digestion method and the histological technique were applied to detect microscopic cysts. It
has been found that esophagus showed the highest rate of infection among the infected organs, with both macroscopic and microscopic
cysts seen in the infected buffaloes. Moreover, results of the pepsin-digestion method proved more accurate than those produced
by the histological technique in terms of infection rates for the microscopic cysts. Our findings indicated that infected
buffaloes aged 2–5 years showed the highest mixed infection rate (82.3%) for both types of cysts. The high prevalence of microscopic
Sarcocystis spp. in Beni-Suef Governorate reflects a significant role played by stray dogs, rather than cats, in the transmission of
these parasites. 相似文献
4.
Nagwan El-Habashi El-Shaymaa El-Nahass Yasuji Namihira Hiroko Hagiwara Hideto Fukushi Minoru Narita Akihiro Hirata Hiroki Sakai Tokuma Yanai 《Journal of Equine Veterinary Science》2011,31(2):72-77
The pathogenicity of equine herpesvirus 9 (EHV-9), a neurotropic equine herpesvirus isolated from a herd of Gazella thomsoni, was studied in cattle. Seven calves were inoculated intranasally with 105 and 107 plaque-forming units of the EHV-9 P19. Three animals showed brain lesions consisting of glial reactions and perivascular cuffings in the olfactory bulb and the frontal and temporal lobes. Additionally, the animal that was inoculated with 107 plaque-forming units showed neuronal degeneration and loss, as well as nuclear inclusions compatible with herpesvirus. EHV-9 was isolated from the brain of calf 6 and the lungs of calves 1 and 2. The results suggested that cattle are susceptible to experimental infection with EHV-9 and at risk from natural infection from reservoir hosts. 相似文献
1