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1.
Swan GE Koeleman HA Steyn HS Mülders MS 《Journal of the South African Veterinary Association》1999,70(2):71-74
The bioavailability of rafoxanide was compared after intraruminal and intra-abomasal administration in healthy adult sheep (n = 6) in a single dose, 2 parallel group study at 7.5 mg/kg. Rafoxanide concentrations in plasma were measured by means of HPLC analysis. Primary pharmacokinetic parameters for bioavailability and disposition of rafoxanide in plasma for both routes of administration were determined by non-compartmental and non-linear, 1-compartmental pharmacokinetic analysis, respectively. Significantly (P < or = 0.05) higher peak plasma concentrations (c(max)) of rafoxanide and a more rapid rate of absorption (c. 3.5 times) was observed in sheep after intra-abomasal (i-a) administration compared to intraruminal (i.r.) administration. A significantly (P < or = 0.05) longer lag period (t(lag)) before absorption (6.8 +/- 2.9 h) occurred after i.r. than after i-a treatment (1.9 +/- 0.6 h). There was no significant difference (P > 0.05) in AUC, MRT and in the rates of elimination (k10-HL and t(1/2beta)) between the i.r. and i-a routes of administration. The results of the study demonstrated the important influence of the rumino-reticulum on absorption of rafoxanide in sheep. 相似文献
2.
Neguvon (Bayer) was administered orally as a therapeutic against the parasitic copepod Lepeophtheirus salmonis. An effective dose is stated and the concentration remaining in fish muscle was measured. 相似文献
3.
Paul S. F. Yip Emmy C. Y. Wan K. S. Chan 《Journal of Agricultural, Biological & Environmental Statistics》2001,6(2):183-194
A unified approach is suggested to estimate the population size for a closed population in discrete time. Individuals can be removed after capture at any time during the experiment. The usual recapture and removal experimentsare shown to be particular cases of the general formulation. The capture probability is assumed to have a logistic function that depends on individual covariates and can be time dependent. The unified approach involves a two-step procedure. A conditional likelihood function is used to estimate the covariates coefficients and a Horvitz-Thompson type estimator to estimate the population size. The asymptotic and small-sample properties of the resulting estimators are in vestigated. A real example is given. 相似文献
4.
To obtain crossing products, an efficient pollination and subsequent fertilization is essential. This efficient pollination is achieved by pollen germination and tube growth. Here, these pollen characteristics of 2 genetically differentiating cultivars of Anemone coronaria L. were investigated in vitro. For the essential components boron, calcium and an osmoticant, only calcium showed to be crucial for pollen germination. Boron concentrations influenced the pollen tube length with a concentration of 100 mg l?1 H3BO3 resulting in the longest pollen tubes. For the osmoticant sucrose, a concentration of 100 g l?1 caused a significant positive effect on both pollen germination and pollen tube length for the 2 cultivars. The cultivars reacted similarly with respect to the investigated compounds. Next to this, the pollen development was correlated with 8 different flower stages in A. coronaria. As a final point, the germination of an optimized pollen germination medium was compared with in vivo pollen germination in cross-pollinations within the same cultivar (identified by aniline blue staining). For ‘Mistral Wine’, pollen germination percentage was lower in vitro than in vivo, while ‘Wicabri Blue’ pollen showed no significant difference in germination rates in vivo and in vitro. To achieve fertilization a following requisite is that the stigma is receptive. To study this, the most receptive female flower stage of the 8 different flower stages for A. coronaria was characterized by aniline blue staining. A. coronaria clearly showed protogyny. 相似文献
5.
Helleborus species are members of the family of the Ranunculaceae. These popular perennials are all diploids (2n = 2x = 32). This study investigates polyploidy induction by different antimitotic agents. Colchicine, oryzalin and trifluralin
were tested in vitro on shoots of Helleborus niger, H. orientalis and H. × nigercors. Furthermore the effect of the antimitotic agents on the viability and the multiplication rate of cultured plantlets were
analyzed. Flow cytometry demonstrated that polyploidisation was genotype dependent: using H. niger, tetraploids were obtained using either oryzalin (3 μM) or trifluralin (3 or 10 μM), whereas for H. × nigercors only trifluralin (3 or 10 μM) induced polyploidisation. For H. orientalis neither treatment was effective to produce tetraploids or mixoploids. For these three species, colchicine (100 μM) was ineffective.
The polyploidisation events in H. niger and H. × nigercors were confirmed by chromosome counts of mounted nuclei derived from root tips (2n = 4x = 64). 相似文献
6.
Emmy Dhooghe Sylvie Denis Tom Eeckhaut Dirk Reheul Marie-Christine Van Labeke 《Euphytica》2009,168(1):33-40
This study investigates the capacity of the antimitotic agents colchicine, oryzalin and trifluralin for inducing polyploidisation
of Ranunculus asiaticus ‘Alfa’ in vitro shoots. Flow cytometry was used to evaluate the optimal concentration of each antimitotic agent for polyploidisation.
Trifluralin at a concentration of 2 μM resulted in the highest percentage of polyploidisation (27.5%), followed by a colchicine
treatment of 200 μM, which induced 23.3% of polyploids. For oryzalin the highest percentage was achieved using a concentration
of 1 μM. Different exposure periods were tested and turned out to be an important factor. The maximal exposure period tested
(10 weeks) resulted in a significant increase in polyploidisation by oryzalin and trifluralin. In contrast, for colchicine
(100 μM) exposure times of either 16 or 24 h did not significantly influence polyploidisation. Additionally the effect of
the antimitotic agents on the viability was analysed. For colchicine no significant effect on the survival rate was observed,
for trifluralin only a concentration of 10 μM affected viability whereas for oryzalin, concentration as well as exposure period
were significant parameters. Flow cytometric data were confirmed by counting chromosomes in root tip cells. 相似文献
7.
Strains of Vibrio anguillarum isolated from rainbow trout (Salmo gairdneri) and saithe (Gadus virens) have been studied and compared, and strains to be used for further investigations are described. 相似文献
8.
Emmy Van Balen 《European journal of plant pathology / European Foundation for Plant Pathology》1982,88(1):33-37
The application of electrical charges on carbon-coated formvar films had a marked effect on the trapping of virus particles by immunosorbent electron microscopical techniques. On grids, positively charged with ethidium bromide, numbers of virus particles were high, and almost equal to those trapped with the aid of protein A in combination with a negative charge.Samenvatting Het aanbrengen van elektrische lading op koolstof-formvar vliezen beïnloedde de adsorptie van virusdeeltjes aan het vlies, bedekt met homoloog antiserum (-globulinen). Op vliezen met een positieve lading werd een veel groter aantal virusdeeltjes waargenomen dan op de neutrale of negatief geladen vliezen. Positief geladen vliezen werden verkregen door behandeling met ethidium-bromide. Negatief geladen vliezen werden verkregen door behandeling met geïoniseerd argongas. Op de negatief geladen vliezen bleken grote hoeveelheden verontreinigingen van allerlei aard te precipiteren. Bovendien was het beeld van de virusdeeltjes vaag. Negatief geladen vliezen gaven wel goede resultaten ten aanzien van het vangen van virusdeeltjes in combinatie met proteine A en -globulinen. 相似文献
9.
蛔虫控制的非药物方法特别受到有机猪生产者的重视。根据荷兰瓦格宁根大学及有关研究中心的一项新研究表明,植物提取物有助于减少猪蠕虫感染。 相似文献
10.
An adult, intact male domestic shorthair presented for preputial swelling and urinary incontinence. A caudal abdominal mass was palpated. A transabdominal ultrasound examination showed severe prostatomegaly with abnormal tissue extending along the urethra. The cat was euthanized due to the owner's financial constraints and the veterinarians' suspicion of a poor long-term prognosis. Biopsies showed chronic active inflammation of the prostate, bladder, kidneys, ureters, penis, and prepuce most consistent with a chronic infectious process. Reports of feline prostatic disease of any kind are rare. Chronic prostatitis may have a more favorable prognosis than feline prostatic adenocarcinoma, currently the most commonly reported disease of the feline prostate. 相似文献