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1.
2.
Urinations of ruminants on grazed pastures increase the risk of nitrate leaching. The study investigated the effect of reducing the length of the grazing season on nitrate leaching from a coarse sandy, irrigated soil during 2006–2007 and 2007–2008. In both years, precipitation was above the long‐term mean. The experiment was initiated in a 4‐yr‐old grass‐clover sward in south Denmark. Three treatments were as follows grazing only (G), spring cut followed by grazing (CG) and both spring and autumn cuts with summer grazing (CGC). Nitrate leaching was calculated by extracting water isolates from 80 cm depth using ceramic suction cups. Because of considerable variation in measured nitrate concentrations, the 32 installed suction cups per treatment were insufficient to reveal differences between treatments. However, weighted nitrate leaching estimations for G, CG and CGC showed estimated mean nitrate N concentrations of 23, 19 and 13 mg/L for an estimated proportion area occupied by urine patches of 0.33, 0.26 and 0.16, respectively. Thus, N concentrations in G and CG exceeded the EU limit of 11.3 mg N/L. Under the prevailing conditions, the time of urination did not appear important. The estimated background leaching calculated from suction cups presumably not situated under urine patches resulted in mean nitrate N concentrations of 2.6 mg/L.  相似文献   
3.

Background

It has been shown that the prevalence of both clinical attachment loss (CAL) ≥1 mm and pocket probing depth (PPD) ≥4 mm is relatively high even in younger dogs, but also that only a minority of the dogs have such clinical signs of periodontal disease (PD) in more than a few teeth. Hence, a minority of dogs carry the major PD burden. These epidemiological features suggest that screening for PD in larger groups of dogs, allowing for rapid assessment of treatment planning, or for the selection of dogs with or without PD prior to be included in experimental trials, should be possible. CAL is the central variable in assessing PD extent and severity while PPD is the central variable used in treatment planning which make these two variables obvious in a screening protocol with the dual aim of disease identification and treatment planning. The main purpose of the present study in 98 laboratory Beagle dogs was to construct a fast, simple and accurate screening tool, which is highly sensitive for the identification of dogs with PD.

Results

Examination of the maxillary P4, P3, P2, I1 and C would, in this population, result in the identification of 85.5% of all dogs and 96% of all teeth positive for CAL ≥1 mm, and 58.9% of all dogs and 82.1% of all teeth positive for PD ≥4 mm.Examination of tooth pairs, all C’s, maxillary I2, M2 and the mandibular P4 would, in this population result in identification of 92.9% of all dogs and 97.3% of all teeth positive for PD ≥4 mm, and 65.5% of all dogs and 83.2% of all teeth positive for CAL ≥1 mm. The results presented here only pertain to the present study population.

Conclusions

This screening protocol is suitable for examination of larger groups of laboratory Beagle dogs for PD and our findings indicate that diseased dogs are identified with a high degree of sensitivity. Before this screening can be used in clinical practice, it has to be validated in breeds other than Beagle dogs and in populations with larger age variation.  相似文献   
4.
  1. While floating near the sea surface plastic debris interacts with a number of external factors, including many different organisms. Seabirds have the most extensive documented history of interactions with plastics, through ingestion, entanglement, and nest construction.
  2. In the present study, eight seabird species from the South Pacific Ocean were used as a proxy to determine potential patterns of removal of marine plastic debris, and three hypotheses were tested in relation to their feeding habits and nesting areas.
  3. Plastics from abiotic compartments (Chilean continental coast, South Pacific Gyre, and Rapa Nui beaches) and biotic compartments (surface-feeding seabirds, diving seabirds, and nesting areas) were compared, according to their type, colour, shape, and density.
  4. Continental beaches had a relatively wide range of colours and shapes, with many non-buoyant plastics. Samples from the South Pacific Gyre (SPG) and Rapa Nui (Easter Island) beaches comprised mainly hard, rounded, buoyant, and white/grey plastics.
  5. These results indicate that the composition of floating plastics from terrestrial sources changes during transport with oceanic currents, reducing the proportion of prey-like plastics present in the subtropical gyres.
  6. The stomach contents of surface-feeding and diving seabirds were dominated by hard, white/grey, and round plastic items, similar to plastics from the SPG, suggesting non-selective (accidental or secondary) ingestion.
  7. Nesting areas had a more variable composition of brightly coloured plastics, suggesting a pattern of selective removal of plastics by seabirds, probably from oceanic sources.
  8. The present study reveals extensive interactions of seabirds with plastics on a broader scale, which is highly relevant given that the impacts of plastics on seabirds are increasing worldwide, compromising their efficient conservation.
  相似文献   
5.
6.
The contribution of animal slurry to the supply of S to plants was studied. The total-S content of cattle and pig slurry was significantly correlated to DM and nitrogen content and was composed of sulfide, sulfate and C-bonded S. During 42 weeks of storage sulfate concentrations decreased and sulfide and C-bonded S concentrations increased. In a pot experiment plant-availability of S in slurry was found to be low, 5 to 7% of total-S.  相似文献   
7.
Abstract. Crops on sandy soils (<5% clay) are exposed to K deficiency due to the small release and high leaching losses of K. Reliable tools are needed to improve the K management in cropping systems with limited K input, such as organic farming where import of nutrients are restricted according to the EC regulations. We investigated K balances and exchangeable K (Kexch) changes in an organic crop rotation experiment. Potassium leaching decreased from 42 kg ha−1 in 1998/99 to 21 kg ha−1 in 2000/01 as an average of a crop rotation (spring barley, grass-clover, winter wheat and pea/barley) with manure application and without catch crops. In the same period, spring Kexch decreased from 5.0 to 3.0 mg K 100 g soil−1 (0–20 cm). The retention of the straw K left in the field after harvest increased with decreasing levels of Kexch. The cereal crops did not respond to K application but in the pea/barley mixture the pea yield increased by 46%. The concordance between measured K balances and changes in Kexch was weak. Exchangeable K is suitable as a tool for K management on a rotational basis, and a Kexch above 3 mg 100 g soil−1 in the autumn should be avoided to minimize K leaching.  相似文献   
8.
Free methanol has been detected and measured, by gas-liquid chromatography, in the breath of several normal, healthy humans. It is suggested that this is a metabolic product rather than the result of diet or intestinal bacterial decomposition although there seems to be no biochemical reason for its presence.  相似文献   
9.
Evidence of estrogen receptors in normal human osteoblast-like cells   总被引:80,自引:0,他引:80  
In seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. The nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. These are characteristics of biologically active estrogen receptors. Pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six cell strains, indicating an induction of functional progesterone receptors. RNA blot analysis demonstrated the presence of messenger RNA for the human estrogen receptor. The data suggest that estrogen acts directly on human bone cells through a classical estrogen receptor-mediated mechanism.  相似文献   
10.
Ascaris suum eggs were collected from pig faeces or dissected from worms obtained from the same pigs. Eggs from the two sources were allowed to embryonate in 0.1 N H2SO4, in 1% buffered formalin or in tap water. The embryonation of the sulphuric acid and water cultures occurred at the same speed, while the formalin cultures developed slightly more slowly. By experimental inoculation of helminth-free pigs and subsequent counting of white spots in the livers and larvae in the lungs day 7 p.i., the infectivity of eggs dissected from worm uteri and embryonated in sulphuric acid (a normal laboratory procedure) was compared with that of eggs collected from faeces and embryonated in water (i.e. more naturally developed eggs). The results suggest that the two types of eggs were equally infective. For this reason the common practice of using Ascaris eggs dissected from worms for experimental infections might be acceptable.  相似文献   
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