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1.
During March 2013, we investigated the presence and the levels of Schmallenberg virus (SBV) circulation in three dairy cow herds and three sheep flocks in Central Macedonia, Greece. In two cow herds, a high number of abortions had been observed during the winter. Six bulk-tank milk samples and 147 individual sera were screened for SBV-specific antibodies by ELISA. Positive reactions were obtained from 5 out of 6 bulk-tank milk samples, 58 out of 90 sera from the 3 cow herds, and 2 sera from 2 of the 3 sheep flocks. Twenty-two ELISA-positive sera were tested by serum neutralization test (SNT). SNT confirmed the presence of neutralizing antibodies against SBV in all samples tested, with titers ranging between 1:32 and ≥1:256. No neutralizing antibodies against Akabane virus (AKAV) or Shamonda virus (SHAV) were detected, indicating that neutralizing antibodies against SBV do not cross react with AKAV or SHAV in SNT. ELISA testing of bulk-tank milk samples proved to be convenient and reliable. None of the tested sera was found positive for SBV by real-time RT-PCR, indicating that the sampling was conducted past the viremia stage. This is the first report of SBV circulation in Greece.  相似文献   
2.
The objective of this study was to investigate the effects of oxytocin infusion on corpus luteum (CL) function during early to mid-diestrus by measuring luteal size (LS) and luteal blood flow (LBF) along with plasma levels of progesterone (P4) and prostaglandin metabolites (13,14-dihydro-15-keto-prostaglandin F, PGFM). On day (D) 7 of the estrus cycle (D1 = ovulation), seven cows received 100 IU of oxytocin (OXY) or placebo (PL) following a Latin square design. LS and LBF increased in both groups over time and no differences were observed between the groups. PGFM did not differ either within the groups over time or between the groups at any time point. P4 of the OXY group was higher compared to that of the the PL group 360 min after the infusion (p = 0.01) and tended to be higher at the time points 450 min, 48 h, and 72 h (all p = 0.08). Results from this study support the hypothesis that OXY is not directly involved in the mechanism(s) governing blood flow of the CL and has no remarkable effects either on luteal size or P4 and PGFM plasma levels. Further investigation is needed to elucidate the role of OXY in CL blood flow during early and late luteal phases.  相似文献   
3.
CASE HISTORY: A routine ultrasonographic examination for pregnancy diagnosis in a 3-year-old ewe revealed a normal embryo located in the uterine cavity and an abnormal mass located in the abdomen. At the time of examination, the ewe was 4.5?months pregnant, but 10?months previously she had undergone a caesarean section due to dystocia.

CLINICAL FINDINGS: The abnormal mass (12?cm × 8?cm) was located outside the uterine cavity; it was anechoic at the periphery, and in the centre there was an echogenic embryo-like structure. Exploratory laparotomy was performed, which revealed an ectopic pregnancy through the caesarean scar.

TREATMENT: The ectopic foetus was excised and the caesarean scar was repaired. The ewe made a complete recovery and the intrauterine pregnancy was completed to full-term, resulting in the birth of a healthy female lamb 35?days after the removal of the ectopic foetus.

CLINICAL RELEVANCE: Ectopic pregnancies have previously been described in ewes. As far as the authors are aware, this is the first report of an ectopic pregnancy through a caesarean scar in sheep or any other farm animal species.  相似文献   
4.
Three new bis azo reactive dyes of different metallic salts (Na, K, Li) were synthesized. The synthesis was obtained by diazotization of 4-amino-2:5 di methoxy phenylene-beta hydroxyl ethyl sulphone sulphate ester and coupling with 1-amino-8-hydroxynaphthalene-3,6-disulfonic acid (H-acid) (moles ratio 2:1). The dyes were purified-concentrated by ultrafiltration technology, characterized and applied on cotton, wool and nylon 6,6 fabric by exhaustion (dyeing) and by ink-jet printing. Fastness properties of the dyeings were measured. Wash fastness was investigated according to international standard methods and was found to be very good to excellent in all cases, while light fastness values were medium to low. Ink-jet ink formulations were prepared using the ultra filtrated reactive blue dyes UF RB(a-c) and their properties pH, conductivity, surface tension and viscosity were monitored over a period of 90 days. The inks were used to print digitally cotton and nylon 6,6 samples and wash- and light fastness properties of the prints were measured. Wash fastness properties were excellent while light fastness values are low Colour measurements of the dyeings and prints were conducted.  相似文献   
5.
Semen availability in ram semen processing facilities is of great importance for the genetic improvement of sheep. Accordingly, any method that would increase sperm viability in low viability ejaculates could be useful. In this study, the possibility of a glass beads filtration method by estimating the beads total surface provided (TSP) for adhesion of spermatozoa, was evaluated. Initially, two different TSP (102 and 154 cm(2)) achieved by various sizes of beads (1500, 2000 and 3000 microm) were tested and no significant difference in sperm viability improvement was noticed for the same TSP by different beads (p > 0.05). Next optimization tests were performed in which three different funnels were used for filtration at a standard TSP (154 cm(2)). The pear-shaped funnel was found to be the most appropriate for filtration, as semen volume recovery and sperm viability improvement were more pronounced (p < 0.05). Finally, filtration tests were conducted with pear-shaped funnels with different TSP (102 and 154 cm(2)) obtained by the aforementioned beads sizes (1500, 2000 and 3000 microm) in equal aliquots. Total surface provided of 102 cm(2) proved to be the more appropriate for filtration than 154 cm(2), as shown by the significant improvement of sperm viability (p < 0.01) and the significantly higher filtrate semen volume (p < 0.05). In conclusion, ram sperm viability improvement by more than 20% of its initial value and semen volume recovery by more than 60%, along with the fact that the total filtration time did not exceed 6 min in any case, suggest that through further development this method could be successfully used during ram semen processing.  相似文献   
6.
The objective of this study was to investigate the quality of frozen‐thawed semen from different bull breeds. Commercial frozen‐thawed bull semen samples (26 per breed, 130 totally) of five breeds (Holstein [Η], Brown Swiss [BS], Limousin [L], Belgian Blue [BB], Blonde d' Aquitaine [BA]) were used. After thawing, each semen sample was subjected to thermal resistance test (TR) for 0.5 and 1 hr at 38°C and hypo‐osmotic swelling test (HOST) for 1 hr at 150 mOsm at 37°C. Additionally, all samples were evaluated at times 0 hr (thawing), 0.5 hr (TR), 1 hr (TR) for kinetics by CASA [progressive, immotile, rapid, medium, slow moving spermatozoa, curvilinear velocity (VCL), average path velocity (VAP), straight line velocity (VSL), linearity (LIN), straightness (STR), beat cross‐frequency (BCF), amplitude of lateral head displacement (ALH), wobble (WOB)]. Moreover, directly after thawing, all semen samples were evaluated for morphometry, morphology, viability and DNA fragmentation. Statistical analysis was conducted using a mixed model for repeated measures. The results showed (a) higher VCL after thawing in H, L breeds compared to BB and BA, (b) higher VAP after thawing in L compared to BB, BA, (c) higher values of progressive spermatozoa after TR in H, BS compared to BB, BA, (d) higher values of rapid spermatozoa after thawing and 0.5 hr of TR in H, BS, L compared to BB, BA, (e) lower viability in BA after thawing compared to H, BS, BB, (f) lower morphological abnormalities in H compared to L, BB, (g) higher head length in Η compared to BB. No significant differences were observed in the results from HOST and DNA fragmentation between breeds. In conclusion, quality characteristics of frozen‐thawed bull semen are dependent on the breed. Frozen semen from BB and BA breeds should be handled more carefully after thawing, as it is more sensitive to stress.  相似文献   
7.
8.
This study estimates the levels and determinants of farm‐level technical efficiency of shrimp farmers, using the stochastic production frontier function involving a model for technical inefficiency effects. A sample of 90 brackish water shrimp farms were surveyed in the region of Shyamnagar Upazila of the Satkhira District, Bangladesh, to study their economic efficiencies. Data were collected, using a questionnaire survey from September to October 2011. The mean technical efficiency level of the shrimp farmers was estimated at 82%, and thus, farmers operate 18% below the production frontier. The results suggest that the inefficiency effect is significant, and that education, age, non‐farm income and distance of the farm are significant determinants of technical inefficiency. By operating at full technical efficiency levels, shrimp production can increase from the current yield of 225.56 to 265.28 kg/ha. Besides improving technical efficiency, technological progress and extension services (such as dissemination of proper information of the technological practices and further adoption of technological practices) can also contribute to increased shrimp production levels. Finally, quality feed and fingerling at affordable prices need to be ensured to further promote shrimp production.  相似文献   
9.
Summary In addition to the deflection due to pure bending in a beam, there is a shear force in all cases of non-uniform bending and a further deflection, due to shear stresses. This additional shear deflection usually is assumed to be negligible and is not considered in computing the total deflection of a beam. In the case of two-species laminated wood beams, due to the development of greater shear stresses, the percentage of shear deflection further increases and failure to take it into consideration may introduce errors of considerable magnitude in determining the distortion of the beam. It is shown here that the percentage of shear deflection can be considerable, approaching the amount due to pure bending. Furthermore it is shown that the magnitude of shear deflection depends on both the span to depth ratio of the beam and the elastic properties of the species involved. It increases as the effective span to depth ratio of the composite beam decreases and as the core ratio of pure modulus of elasticity to modulus of rigidity increases.
Zusammenfassung Zu der Verformung eines Balkens unter reiner Biegung tritt bei der Einwirkung ungleichförmiger Biegekräfte eine zusätzliche Verformung, die durch eine Schubkraft und die daraus resultierenden Schubspannungen verursacht wird. Von dieser zusätzlichen Schubverformung nimmt man gewöhnlich an, daß sie vernachlässigt werden kann. Sie wird daher bei der Berechnung der Gesamtverformung eines Balkens nicht mit berücksichtigt. Im Falle von Schichtholzbalken aus zwei verschiedenen Holzarten erhöht sich der Anteil der Schubverformung, verursacht durch das Auftreten größerer Schubspannungen noch weiter, und die Vernachlässigung kann bei der Bestimmung der Gesamtformveränderung zu beträchtlichen Fehlern führen. Es wird gezeigt, daß der Anteil der Schubverformung hoch sein und sich der Größe der reinen Biegeverformung deutlich nähern kann. Es wird ferner nachgewiesen, daß die Größe der Schubverformung vom Verhältnis der Stützweite zur Dicke des Balkens und von den elastischen Eigenschaften der verwendeten Holzarten abhängt. Die Schubverformung erhöht sich, wenn das wirksame Verhältnis von Spannweite zu Dicke des Schichtholzbalkens ansteigt und wenn das Verhältnis zwischen reinem E-Modul und Schubmodul ansteigt.


The experimental work of this study was conducted at the Yale School of Forestry in cooperation with the Office of Naval Research. Department of the Navy, under contract No. 609 (13), Project NR 330-001, Properties of Tropical Woods.  相似文献   
10.
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