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Arno Lindner Raul Signorini Jorge Vassallo Fabiana Tomatis Francis M. Flores María E. Gagliano Jimena Curiotti Evelina Terragona 《Journal of Equine Veterinary Science》2010
This study examined the reproducibility and repeatability of muscle thickness (MT) measurements with ultrasound for the following muscles: extensor carpi radialis, extensor digitorum longus (both flexed and extended), gluteus medius, longissimus lumborum, semitendinosus, and supraspinatus. Three examiners measured thickness of these muscles in five Thoroughbreds on 3 consecutive days. The day of measurement did not have any effect on the mean MT value of the muscles examined (P > .05). The left longissimus lumborum muscle was the only muscle for which the second measurement varied from the first and third (P < .05). The examiners had an effect on the mean coefficient of variation (CV) of the thickness of the flexed left extensor carpi radialis and flexed left extensor digitorum longus. The daily measurements varied more in the case of one of the examiners. Mean CVs higher or close to 10% were reported for both sides of the flexed extensor digitorum longus and for the supraspinatus muscles. The lowest CV was calculated for the longissimus lumborum and the extended semitendinosus (<5%). The largest disagreements between the examiners were observed for both sides of the flexed extensor digitorum longus and the supraspinatus (>10%–<20%). The best agreement was measured for the extended extensor carpi radialis and the longissimus lumborum (>3%–<7%). The results showed that for some muscles, it was difficult to locate the exact anatomical site for taking the MT measurements. To reduce CV, it was suggested that only one examiner should take all the measurements and the whole procedure must be such that it is as comfortable as possible for this particular examiner. 相似文献
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Connectivity providers for semi-aquatic vertebrates: the case of the endangered otter in Italy 总被引：1，自引：0，他引：1
Maria Laura Carranza Evelina D’Alessandro Santiago Saura Anna Loy 《Landscape Ecology》2012,27(2):281-290
Modeling habitat connectivity for conservation of semi-aquatic vertebrates is a particularly challenging task, due to the
fine-scale and linear distribution of riverine habitats and to the capacity of species to move both on freshwater and terrestrial
realms. We showed how the integrated analytical framework provided by the habitat availability (reachability) metrics and
their fractions can be used to effectively evaluate the distinctive roles and contributions of both habitat patches (aquatic
and riparian) and linkage areas (permeable land matrix) to the connectivity and functioning of a complex system composed of
multiple river catchments. Analysis focused on the Eurasian otter (Lutra lutra L.), one of the most endangered mammals in Italy. We developed a network connectivity model based on suitable otter habitats
and multiple least-cost paths between catchments. A graph analytical approach was used to identify critical nodes and links
for the potential expansion and long-term viability of the species in the region. Our results showed that few basins concentrate
most of the importance for sustaining the overall habitat connectivity, due to the extension of suitable habitats they contain,
their strong connections with other basins, and their importance as stepping stones that uphold ecological fluxes between
otherwise weakly connected habitats. The potential contribution of each basin to enhance the dispersal and expansion of otters
in the area strongly depended on the key functional paths (sequences of links and nodes) among the catchments. We identified
vacant basins that could be colonized by otters in the near future, and connecting areas in the intermediate matrix that might
be preferentially used to conduct and promote dispersal movements and gene flow in the area. The novel approach here adopted
could be easily extended to other semiaquatic species and catchment systems, offering a management strategy to preserve the
hydrographic network as an integrated system, as well as a joint evaluation of the role of both the river courses and the
matrix in between in a single landscape model. 相似文献
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The effects of soil disturbance and residue retention on the functionality of the symbiosis between medic (Medicago truncatula L.) and arbuscular mycorrhizal fungi (AMF) were assessed in a two-stage experiment simulating a crop rotation of wheat (Triticum aestivum L.) followed by medic. Plants were inoculated or not with the AMF, Glomus intraradices and Gigaspora margarita, separately or together. The contribution of the arbuscular mycorrhizal (AM) pathway for P uptake was determined using 32P-labeled soil in a small hyphal compartment accessible only to hyphae of AMF. In general AM colonization was not affected by soil disturbance or residue application and disturbance did not affect hyphal length densities (HLDs) in soil. At 4 weeks disturbance had a negative effect on growth and phosphorus (P) uptake of plants inoculated with G. margarita, but not G. intraradices. By 7 weeks disturbance reduced growth of plants inoculated with G. margarita or AMF mix and total P uptake in all inoculated plants. With the exception of plants inoculated with G. margarita in disturbed soil at 4 weeks, the AM pathway made a significant contribution to P uptake in all AM plants at both harvests. Inoculation with both AMF together eliminated the negative effects of disturbance on AM P uptake and growth, showing that a fungus insensitive to disturbance can compensate for loss of contribution of a sensitive one. Application of residue increased growth and total P uptake of plants but decreased 32P in plants inoculated with the AMF mix in disturbed soil, compared with plants receiving no residue. The AMF responded differently to disturbance and G. intraradices, which was insensitive to disturbance, compensated for lack of contribution by the sensitive G. margarita when they were inoculated together. Colonization of roots and HLDs in soil were not good predictors of the outcomes of AM symbioses on plant growth, P uptake or P delivery via the AM pathway. 相似文献
5.
Protective effect of the phenolic fraction from virgin olive oils against oxidative stress in human cells 总被引：3，自引：0，他引：3
Manna C D'Angelo S Migliardi V Loffredi E Mazzoni O Morrica P Galletti P Zappia V 《Journal of agricultural and food chemistry》2002,50(22):6521-6526
This paper reports the protective effect of the phenolic fraction extracted from extra virgin olive oils (OOPEs) against the cytotoxic effects of reactive oxygen species in human erythrocytes and Caco-2 cells, employed as model systems. Pretreatment of cells with various OOPEs, indeed, provides a remarkable protection against oxidative damages: this effect was strictly dependent on the o-diphenolic content of the extracts. Moreover, the protective effects observable in cellular systems were compared with in vitro antioxidant properties, measured by using the FRAP (ferric reducing/antioxidant power) assay; the reducing ability of OOPEs strictly parallels their o-phenolic content. The linear relationship demonstrated between biological effects and antioxidant capacity measured by the FRAP assay allows us to propose the use of this rapid colorimetric method in assessing and certifying the antioxidant power of extra virgin olive oil. 相似文献
6.
Silvia Viviana Soriano Nora Beatriz Pierangeli Irene Roccia Hector Fabián Jesus Bergagna Lorena Evelina Lazzarini Alejandra Celescinco Mónica Susana Saiz Alejandra Kossman Pablo Adrián Contreras Cecilia Arias Juan Angel Basualdo 《Veterinary parasitology》2010
The presence of parasites was investigated by the examination of 1944 dog faecal samples collected from urban (n = 646) and rural (n = 1298) areas of the province of Neuquén, Patagonia, Argentina. Parasitic agents (PA) were found in 37.86% of samples. A total of 15 different PA were detected, including Toxocara canis (16.35%), Taenia spp./Echinococcus spp. (12.65%), Trichurisvulpis (6.06%), Giardia spp. (1.29%), Toxascaris leonina (0.56%), Ancylostomacaninum (0.41%), Dipylidium caninum (0.31%), Diphyllobothrium spp. (0.10%), among others. Several of these PA are recognized as zoonotic agents. Therefore, the results of this investigation revealed that local population is exposed to a broad spectrum of zoonotic parasites by means of environmental contamination with dog faeces. Prevalence of PA was slightly higher in rural (40.06%) than in urban (33.44%) locations. Distribution of groups of PA (cestodes, nematodes, and protozoa) showed statistical differences between both habitats. Prevalence of cestodes (18.18%) and protozoa (11.86%) was significantly higher in the rural environment than in urban areas and nematodes (29.10%) were more frequent in urban locations. Infection of dogs with Linguatula serrata and Cryptosporidium sp. was demonstrated for the first time in Neuquén. Rural dogs of the study area are under hydatic disease control program, which includes treatment with praziquantel every 6 weeks; thus, the finding of high level of cestode infection in these areas is of great relevance. The epidemiology of zoonotic parasitic infections in urban and rural dogs showed different patterns and, in consequence, different control measurements should be applied in each location. 相似文献
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Linlaud N Ferrer E Puppo MC Ferrero C 《Journal of agricultural and food chemistry》2011,59(2):713-719
Interaction of hydrocolloids (xanthan gum, locust bean gum, guar gum, and high-methoxyl pectin) with macrocomponents of dough (water, starch, and protein) was evaluated by different techniques. (1)H spin-spin NMR relaxation assays were applied to study the mobility of the gluten-hydrocolloid-water matrix, and the amount of freezable water was determined by differential scanning calorimetry (DSC). Starch gelatinization parameters (T, enthalpy) were also analyzed by DSC. The influence of additives on the protein matrix was studied by Fourier transform (FT) Raman assays; analysis of the extracted gliadins and glutenins was performed by electrophoresis (SDS-PAGE). A significantly higher molecular mobility was found in matrices containing xanthan gum, whereas pectin led to the lowest molecular mobility. Freezable water showed a trend of increasing in the presence of hydrocolloids, particularly under conditions of water restriction. Starch gelatinization final temperature was decreased when hydrocolloids were added in the presence of enough water. In general, FT-Raman and SDS-PAGE indicated that hydrocolloid addition promoted a more disordered and labile network, particularly in the case of pectin addition. On the other hand, results obtained for dough with guar gum would indicate a good compatibility between this hydrocolloid and the gluten network. 相似文献
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Children's exposure to Di(2-ethylhexyl)phthalate and dibutylphthalate plasticizers from school meals
Cirillo T Fasano E Castaldi E Montuori P Amodio Cocchieri R 《Journal of agricultural and food chemistry》2011,59(19):10532-10538
Packed school meals for children 3-10 years old were studied to evaluate the levels of di(2-ethylhexyl)phthalate (DEHP) and di-n-butylphthalate (DBP) and the influence of the packaging process on meal contamination, and their contribution to daily intake was estimated. The packaging consisted of polyethylene-coated aluminum (PE/Al) dishes thermally welded by a polyethyleneterephthalate-coated aluminum (PET/Al) foil. Foodstuffs before processing were analyzed, too. Total meals before packaging and after packaging were collected. It was found that 92% of foodstuffs employed in meal preparation contained DEHP, and 76% of them DBP, at detectable levels. In cooked foods before packaging the DEHP median concentration levels varied from 111.4 to 154.8 ng/g ww and those of DBP between 32.5 and 59.5 ng/g ww. In packed meals the DEHP median values ranged from 127.0 to 253.3 ng/g ww, and DBP median values varied from 44.1 to 80.5 ng/g ww. The mean increases of median concentrations of DEHP in cooked foods before and after packaging were 113 and 125% for DBP. For nursery and primary school children DEHP intake via school meals can raise on average the respective EFSA TDI by 18 and 12% and that of DBP by 50 and 30%. 相似文献