Comparative virulence of Haemophilus parasuis serovars 1 to 7 in guinea pigs.     

V J Rapp-Gabrielson  D A Gabrielson  G J Schamber 《American journal of veterinary research》1992,53(6):987-994

Reference strains for Haemophilus parasuis serovars 1 to 7 were examined for virulence by inoculation of guinea pigs. Guinea pig response to intraperitoneal inoculation was similar for the 7 reference strains. However, apparent differences in virulence were detected after intratracheal inoculation. Cells of the references strains for serovars 1 and 5 were most invasive, causing moribundity or death at higher doses and a persistent septicemia at lower doses. Haemophilus parasuis could be isolated from respiratory and systemic sites; purulent bronchopneumonia, pericarditis, and pleuritis were apparent in infected guinea pigs. Inoculation of cells of the reference strains for serovars 2 and 6 also resulted in bronchopneumonia and moribundity or death in some guinea pigs; however, reisolation of H parasuis and microscopic lesions at necropsy were less pronounced than those observed with serovars 1 and 5. Inoculation of cells of serovars 3, 4 and 7 induced only transient clinical signs and minimal evidence of H parasuis infection at necropsy. The data from intratracheal inoculation of guinea pigs are similar to data from other investigations in swine, indicating differences in the pathogenic potential of H parasuis strains. Thus, guinea pigs may be useful as a laboratory animal model for examining cellular factors associated with virulence and immunogenicity of H parasuis.  相似文献   

Verbesserung der Krankheitsresistenz von Kohlgemüse: 2. Kohlhernie, Alternaria- und Phoma-Blattfleckenkrankheit     

Paul Scholze  Reiner Krämer  Frank Marthe  Ulrich Ryschka  Evelyn Klocke  Günter Schumann 《Gesunde Pflanzen》2003,55(7):199-204

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Pneumocystosis associated with canine distemper virus infection in a mink          下载免费PDF全文

Dyer NW  Schamber GJ 《The Canadian veterinary journal. La revue veterinaire canadienne》1999,40(8):577-578

An adult mink from a farm experiencing 100% mortality in affected animals was submitted for diagnostic examination. Clinical history included signs of respiratory disease, oculonasal discharge, and thickening of footpads. Canine distemper virus and Pneumocystis carinii were identified in lung tissue, suggesting immunosuppresion and secondary infection due to morbillivirus disease.  相似文献   

Osteomyelitis associated with an orthopedic implant          下载免费PDF全文

Rahal SC  Mamprim MJ  Caporali EH  Teixeira CR 《The Canadian veterinary journal. La revue veterinaire canadienne》2003,44(7):597-599

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Blood lead levels of wild Steller's eiders (Polysticta stelleri) and black scoters (Melanitta nigra) in Alaska using a portable blood lead analyzer.     

Corrine S Brown  Joanne Luebbert  Daniel Mulcahy  Jason Schamber  Daniel H Rosenberg 《Journal of zoo and wildlife medicine》2006,37(3):361-365

Sea duck populations are declining in Alaska. The reasons for the decline are not known; environmental lead exposure is one suspected cause. Thirty wild Steller's eider ducks (Polysticta stelleri) and 40 wild black scoter ducks (Melanitta nigra) were tested for blood lead levels using a portable blood lead analyzer (LeadCare; ESA, Inc., Chelmsford, Massachusetts 01824, USA). Sixty-seven and one-tenth percent of the sea ducks had undetectable blood lead levels, 30.0% had values indicating normal or background lead exposure, and 2.9% had values indicating lead exposure. None of the birds had values indicating lead toxicity, and no birds demonstrated clinical signs of toxicity. Birds in areas with higher human population density had higher blood lead levels than those in less densely populated areas. This is the first time a portable blood lead analyzer has been utilized with sea ducks in a field setting. Because it provides immediate results, it is valuable as a screening tool for investigators carrying out surgical procedures on birds in the field as well as establishing baseline blood lead data on sea ducks. Lead exposure does occur in wild sea ducks, and the study indicates that additional research is needed in order to determine the role environmental lead plays in declining sea duck populations.  相似文献   

A synthesis of the life history and ecology of juvenile Pacific herring in Prince William Sound,Alaska     

Brenda L. Norcross  Evelyn D. Brown  Robert J. Foy  Michele Frandsen  Shelton M. Gay  Thomas C. Kline  Doran M. Mason  E. Vincent Patrick  A. J. Paul  Kevin D. E. Stokesbury 《Fisheries Oceanography》2001,10(Z1):42-57

Physical and biological variables affecting juvenile Pacific herring (Clupea pallasi) in Prince William Sound (PWS) from 1995 to 1998 were investigated as part of a multifaceted study of recruitment, the Sound Ecosystem Assessment (SEA) program. Though more herring larvae were retained in eastern PWS bays, ages‐0 and ‐1 herring used bays throughout PWS as nursery areas. Water transported into PWS from the Gulf of Alaska (GOA) contributed oceanic prey species to neritic habitats. Consequently, variations in local food availability resulted in different diets and growth rates of herring among bays. Summer food availability and possible interspecific competition for food in nursery areas affected the autumn nutritional status and juvenile whole body energy content (WBEC), which differed among bays. The WBEC of age‐0 herring in autumn was related to over‐winter survival. The limited amount of food consumption in winter was not sufficient to meet metabolic needs. The smallest age‐0 fish were most at risk of starvation in winter. Autumn WBEC of herring and winter water temperature were used to model over‐winter mortality of age‐0 herring. Differences in feeding and energetics among nursery areas indicated that habitat quality and age‐0 survival were varied among areas and years. These conditions were measured by temperature, zooplankton abundance, size of juvenile herring, diet energy, energy source (GOA vs. neritic zooplankton), WBEC, and within‐bay competition.  相似文献   

Microbiology. Detoxifying enzyme helps animals stomach bacteria     

Strauss E 《Science (New York, N.Y.)》2007,318(5858):1853-1854

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Rural Women and Economic Development     

Evelyn R. Entwistle 《Asia Pacific viewpoint》1985,26(2):437-447

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Pacific flows: The fluidity of remittances in the Cook Islands     

Evelyn Marsters  Nick Lewis  Wardlow Friesen 《Asia Pacific viewpoint》2006,47(1):31-44

Abstract: This paper contributes political and cultural‐economy perspectives to the critique of the MIRAB model 20 years on. In it, we celebrate the politically grounded reading by MIRAB analysts of development in the small island nations of the Pacific and their attention to both the empirical and the structural in their treatment of the economies of these countries. We address aspects, however, of one of the common critiques of MIRAB analyses: their failure to capture accurately the nature of small island socio‐cultural economies. We focus on the workings of remittance systems on two of the Cook Islands, Mauke and Manihiki, as the basis for a more thorough critique. We argue that rather than living economically and nationally determined lives, Cook Islanders live in rich networks of flows of goods, people, labour and meaning that the MIRAB model does not fully capture. The microeconomics of the transnational kin or household unit and the remittance decision are deeply embedded in such networks. These networks generate their own, temporary constellations of responsibility, economy and decision‐making, which may or may not materialise at any point as household economy. We consider some of the consequences of a network view for MIRAB analyses and for development in small island nations.  相似文献   

8S globulin of mungbean [Vigna radiata (L.) Wilczek]: cloning and characterization of its cDNA isoforms, expression in Escherichia coli, purification, and crystallization of the major recombinant 8S isoform     

Bernardo AE  Garcia RN  Adachi M  Angeles JG  Kaga A  Ishimoto M  Utsumi S  Tecson-Mendoza EM 《Journal of agricultural and food chemistry》2004,52(9):2552-2560

Three isoforms of the cDNA of the major 8S globulin of mungbean, 8Salpha, 8Salpha', and 8Sbeta, were isolated, cloned, and characterized. The cDNA sequences of 8Salpha, 8Salpha', and 8Sbeta had open reading frames of 1362, 1359 or 1362, and 1359 bp, respectively, which code for 454, 453 or 454, and 453 amino acids corresponding to molecular weights of 51 973, 51 627 or 51 758, and 51 779, respectively. Homology in terms of cDNA and amino acid sequences was 91-92% between 8Salpha and 8Salpha', 87% between 8Salpha and 8Sbeta, and 86-88% between 8Salpha' and 8Sbeta. The signal peptide was found to be 1-25, 1-24 or 25, and 1-23 for 8Salpha, 8Salpha', and 8Sbeta, respectively, using the signalP website (Nielsen, H.; Engelbrecht, J.; Brunak, S.; von Heijne, G. Protein Eng. 1997, 10, 1-6). The propeptide was determined to be IVHREN. A single site for glycosylation (N-X-S/T) was observed about 90 amino acids from the C terminus. Homology between mungbean 8S isoforms and other 7-8S proteins ranged from 45 to 68% within members of the legume family and 29 to 34% for crops of different species. The major isoform 8Salpha was expressed in Escherichia coli and purified by successive ammonium sulfate fractionation, hydrophobic interaction, and Mono Q column chromatography. The recombinant 8Salpha, but not the native form, was successfully crystallized producing rhombohedral crystals.  相似文献