Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil     

R.L. de Miranda  L.H. O'DwyerJ.R. de Castro  B. MetzgerA.S. Rubini  A.V. MundimO. Eyal  D. Talmi-FrankM.C. Cury  G. Baneth 《Research in veterinary science》2014

The objective of this survey was to investigate the prevalence of Hepatozoon infection in dogs in the rural and urban areas of Uberlândia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR products from positive blood samples of urban dogs and 13 from the rural dogs were sequenced. Partial sequences of the 18S rRNA gene indicated that all 30 dogs were infected with Hepatozoon canis similar in sequence to H. canis from southern Europe. Four local dog sequences were submitted to GenBank (accessions JN835188; KF692038; KF692039; KF692040). This study indicates that H. canis is the cause of canine hepatozoonosis in Uberlândia and that infection is similarly widespread in rural and urban dogs.  相似文献   

Molecular biology of male gametogenesis     

Sheila Mccormick  Catherine Curie  Yoram Eyal  Jorge Muschietti  Lori Dircks  Rima Kulikauskas 《Euphytica》1994,79(3):245-250

Pollen development has been studied at a molecular level in several systems that are amenable to genetic or transgenic analysis. We have characterized several tomato genes that are expressed late in pollen development. Our goals in this research were 1) to determine the cis- and tran-acting factors that mediate pollen expression, and 2) to determine the functions of the proteins encoded by these genes. We currently favor the hypothesis that pollen-specific gene expression is mediated in a combinatorial manner. Antisense experiments have indicated an important role for the LAT52 protein during pollen hydration.  相似文献   

Variations in pathogenicity patterns ofMycosphaerella graminicola withinTriticum spp. in Israel     

Z. Eyal  E. Levy 《Euphytica》1987,36(1):237-250

Summary Pathogenicity patterns of 42Mycosphaerella graminicola (Septoria tritici) isolates secured from the major wheat growing regions in Israel, were assessed on seedlings of 16 bread and durum wheats. The spring bread wheat cultivar Titan (CI12615), the winter bread wheats Bezostaya 1, Kavkaz, NE7060 and the durum wheats Nursit 163 and Zenati Bouteille, all exhibited a high level of resistance. Significant cultivar × isolate interactions were recorded for isolates from the different regions in relation to the response of the 16 cultivars organized in 6 reponse classes to the test isolate ISR8036. Number of genes for resistance in the 16 cultivars was estimated, based on the assumption of a gene-for-gene relationship. Twelve complementary genes were hypothesized in the 42M. graminicola isolates × 16 wheat cultivars matrix. There was considerable variation in virulence frequencies between regions and between locations within the same region. The overall frequency of Bezostaya-Kavkaz virulence was low throughout the country (5–7%). The frequency of designated hypothesized virulence genes VST OLAF, VST COLOTANA, VST IAS 20 in Israel, was high in most locations. The reported analytical approach enables the identification of virulence hot-spots and assists in designing breeding for resistance strategies.  相似文献   

Zooming in on a quantitative trait for tomato yield using interspecific introgressions   总被引：2，自引：0，他引：2  

Fridman E  Carrari F  Liu YS  Fernie AR  Zamir D 《Science (New York, N.Y.)》2004,305(5691):1786-1789

To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.  相似文献   

Mechanism of Na+/H+ antiporting     

Arkin IT  Xu H  Jensen MØ  Arbely E  Bennett ER  Bowers KJ  Chow E  Dror RO  Eastwood MP  Flitman-Tene R  Gregersen BA  Klepeis JL  Kolossváry I  Shan Y  Shaw DE 《Science (New York, N.Y.)》2007,317(5839):799-803

Na+/H+ antiporters are central to cellular salt and pH homeostasis. The structure of Escherichia coli NhaA was recently determined, but its mechanisms of transport and pH regulation remain elusive. We performed molecular dynamics simulations of NhaA that, with existing experimental data, enabled us to propose an atomically detailed model of antiporter function. Three conserved aspartates are key to our proposed mechanism: Asp164 (D164) is the Na+-binding site, D163 controls the alternating accessibility of this binding site to the cytoplasm or periplasm, and D133 is crucial for pH regulation. Consistent with experimental stoichiometry, two protons are required to transport a single Na+ ion: D163 protonates to reveal the Na+-binding site to the periplasm, and subsequent protonation of D164 releases Na+. Additional mutagenesis experiments further validated the model.  相似文献   

Ringlike structure of the Deinococcus radiodurans genome: a key to radioresistance?     

Levin-Zaidman S  Englander J  Shimoni E  Sharma AK  Minton KW  Minsky A 《Science (New York, N.Y.)》2003,299(5604):254-256

The bacterium Deinococcus radiodurans survives ionizing irradiation and other DNA-damaging assaults at doses that are lethal to all other organisms. How D. radiodurans accurately reconstructs its genome from hundreds of radiation-generated fragments in the absence of an intact template is unknown. Here we show that the D. radiodurans genome assumes an unusual toroidal morphology that may contribute to its radioresistance. We propose that, because of restricted diffusion within the tightly packed and laterally ordered DNA toroids, radiation-generated free DNA ends are held together, which may facilitate template-independent yet error-free joining of DNA breaks.  相似文献   

Metabolic engineering of plant cells for biotransformation of hesperedin into neohesperidin, a substrate for production of the low-calorie sweetener and flavor enhancer NHDC     

Frydman A  Weisshaus O  Huhman DV  Sumner LW  Bar-Peled M  Lewinsohn E  Fluhr R  Gressel J  Eyal Y 《Journal of agricultural and food chemistry》2005,53(25):9708-9712

Neohesperidin dihydrochalcone (NHDC) is a seminatural, safe, low-calorie sweetener, bitterness blocker, and flavor enhancer with unique properties and applications for the food, beverage, pharmaceutical, and animal feed industries. Current production is limited by the availability of the substrate neohesperidin, a flavonoid that accumulates to significant levels only in the inedible bitter citrus species. We propose a process to convert hesperidin, a tasteless flavonoid extracted from orange peels that are abundant byproducts of the vast orange juice industry, into neohesperidin using metabolic engineering and biotransformation via three steps: (i) extraction of hesperidin from orange peels, (ii) hydrolysis of sugar moieties, and (iii) biotransformation of hesperidin hydrolysis products into neohesperidin. We overcame the current technological bottleneck in biotransformation of hesperidin hydrolysis products into neohesperidin using metabolically engineered plant cell cultures expressing a recombinant flavanone-7-O-glucoside-2-O-rhamnosyltransferase. A small-scale production experiment established the feasibility of the proposed process.  相似文献   

Abstracts of papers presented at the Meeting of the Phytopathological Society of israel     

Z. R. Frank  O. Ziv  Z. Eyal  E. Galun  D. Netzer  S. Niego  Z. Zuta  Ch. Davidi  Yigal Cohen  M. Pilowsky  D. Globerson  Tova Sadon 《Phytoparasitica》1976,4(3):207-210

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Demographic and environmental risk factors for infection by Theileria equi in 590 horses in Israel     

Steinman A  Zimmerman T  Klement E  Lensky IM  Berlin D  Gottlieb Y  Baneth G 《Veterinary parasitology》2012,187(3-4):558-562

The prevalence of Theileria equi infection as well as the environmental and demographic risk factors for infection was studied in 590 healthy horses from 46 farms in Israel. The prevalence of T. equi DNA was assessed using a polymerase chain reaction for a segment of the Theileria 18S rRNA gene. The overall prevalence was 26.4% (156/590). There was a significant geographical variation in the prevalence of T. equi infection, ranging from 9.3% (25/270) in the central lowlands to 81.7% (49/60) in the Golan Heights. The prevalence of T. equi infection was found to be significantly associated with management types with more horses with access to pasture being positive. Breed was identified as a risk factor for T. equi infection in a univariate analysis with relatively high infection rates in the Quarter horse and local breeds (41.1% and 36.3% respectively), while ponies and Arabian horses had a relatively low prevalence (10% and 9.1%, respectively). However, since a correlation between geographic location and breed was found, it is difficult to draw definite conclusions regarding this risk factor. Age and gender were not found as risk factors for T. equi infection in this study. The environmental variables that were significantly associated with positivity were relative humidity and minimum land surface temperature at day which both showed negative correlation with T. equi prevalence. In conclusion, Israel was found to be enzootic for T. equi infection, as indicated by the high sub-clinical infection rate, which differed between geographical areas.  相似文献   

Canine procalcitonin messenger RNA expression     

Sharon Kuzi  Itamar Aroch  Keren Peleg  Ohad Karnieli  Eyal Klement  Gillian Dank 《Journal of veterinary diagnostic investigation》2008,20(5):629-633

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