全文获取类型
收费全文 | 3564篇 |
免费 | 169篇 |
国内免费 | 14篇 |
专业分类
林业 | 298篇 |
农学 | 215篇 |
基础科学 | 22篇 |
943篇 | |
综合类 | 332篇 |
农作物 | 347篇 |
水产渔业 | 355篇 |
畜牧兽医 | 866篇 |
园艺 | 110篇 |
植物保护 | 259篇 |
出版年
2024年 | 4篇 |
2023年 | 36篇 |
2022年 | 75篇 |
2021年 | 175篇 |
2020年 | 167篇 |
2019年 | 167篇 |
2018年 | 178篇 |
2017年 | 162篇 |
2016年 | 173篇 |
2015年 | 173篇 |
2014年 | 187篇 |
2013年 | 246篇 |
2012年 | 309篇 |
2011年 | 284篇 |
2010年 | 194篇 |
2009年 | 196篇 |
2008年 | 227篇 |
2007年 | 205篇 |
2006年 | 167篇 |
2005年 | 93篇 |
2004年 | 80篇 |
2003年 | 57篇 |
2002年 | 52篇 |
2001年 | 20篇 |
1999年 | 7篇 |
1998年 | 6篇 |
1997年 | 9篇 |
1996年 | 6篇 |
1995年 | 7篇 |
1994年 | 7篇 |
1993年 | 10篇 |
1992年 | 6篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1985年 | 3篇 |
1984年 | 7篇 |
1981年 | 4篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1973年 | 2篇 |
1970年 | 3篇 |
1964年 | 2篇 |
1963年 | 2篇 |
1959年 | 2篇 |
1936年 | 2篇 |
1928年 | 2篇 |
1863年 | 1篇 |
1860年 | 1篇 |
1857年 | 2篇 |
排序方式: 共有3747条查询结果,搜索用时 125 毫秒
1.
Ana E. González-Santiago Belinda Vargas-Guerrero Pedro M. García-López Alma L. Martínez-Ayala José A. Domínguez-Rosales Carmen M. Gurrola-Díaz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(2):134-140
Lupinus albus seeds contain conglutin gamma (Cγ) protein, which exerts a hypoglycemic effect and positively modifies proteins involved in glucose homeostasis. Cγ could potentially be used to manage patients with impaired glucose metabolism, but there remains a need to evaluate its effects on hepatic glucose production. The present study aimed to analyze G6pc, Fbp1, and Pck1 gene expressions in two experimental animal models of impaired glucose metabolism. We also evaluated hepatic and renal tissue integrity following Cγ treatment. To generate an insulin resistance model, male Wistar rats were provided 30% sucrose solution ad libitum for 20 weeks. To generate a type 2 diabetes model (STZ), five-day-old rats were intraperitoneally injected with streptozotocin (150 mg/kg). Each animal model was randomized into three subgroups that received the following oral treatments daily for one week: 0.9% w/v NaCl (vehicle; IR-Ctrl and STZ-Ctrl); metformin 300 mg/kg (IR-Met and STZ-Met); and Cγ 150 mg/kg (IR-Cγ and STZ-Cγ). Biochemical parameters were assessed pre- and post-treatment using colorimetric or enzymatic methods. We also performed histological analysis of hepatic and renal tissue. G6pc, Fbp1, and Pck1 gene expressions were quantified using real-time PCR. No histological changes were observed in any group. Post-treatment G6pc gene expression was decreased in the IR-Cγ and STZ-Cγ groups. Post-treatment Fbp1 and Pck1 gene expressions were reduced in the IR-Cγ group but increased in STZ-Cγ animals. Overall, these findings suggest that Cγ is involved in reducing hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders. 相似文献
2.
3.
Mark D. Rawling Nicola Pontefract Ana Rodiles Ilektra Anagnostara Eric Leclercq Marion Schiavone Mathieu Castex Daniel L. Merrifield 《Journal of the World Aquaculture Society》2019,50(6):1108-1122
Fish were fed a single‐strain yeast fraction (SsYF; 2 g/kg) or a multistrain yeast fraction (MsYF; 0.8 g/kg) for 10 weeks. The results demonstrated significant (p ≤ 0.03) elevations in weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio in fish fed the yeast fraction‐supplemented diets. In the distal intestine, a significant elevation in microvilli density was observed after 5 and 10 weeks of dietary supplementation with MsYF and SsYF, respectively, compared to control fed fish (p < 0.001). A significant elevation (p = 0.02) in the perimeter ratio was observed in fish fed diets supplemented with the yeast fractions. After 10 weeks of feeding on the experimental diets, Rt‐qPCR demonstrated a significant downregulation (p < 0.05) in the stress response genes, heat‐shock protein 70 (hsp70) and proliferating cell nuclear antigen (pcna), in fish fed diets supplemented with the yeast fractions. Significant (p < 0.05) elevations in interleukin 1‐beta (il1β) and interleukin‐10 (il10) gene expression were observed in fish fed diets supplemented with the MsYF compared to the other dietary groups. These findings suggest that feeding an MsYF specifically at a lower incorporation rate < 1 g/kg, compared to a commercial SsYF at 2 g/kg, is effective in improving the intestinal health status and growth performance of European seabass. 相似文献
4.
Definitions of the notions of soil resilience and degradation of the soil-vegetation cover are suggested. These problems are considered with respect to vulnerable tundra ecosystems. The analysis of available experimental data made it possible to obtain empirical equations characterizing the relationships between the plant productivity and the soil properties. These equations can be used to predict the degree of soil degradation under particular types of technogenic loads and the soil and vegetation potential for the natural restoration or artificial rehabilitation of the soil-vegetation cover in tundra ecosystems. The results obtained can be applied for the development of optimal nature management strategies in the areas of oil and gas fields in the north. 相似文献
5.
6.
Alla M. Gol’Berg Shoshana Yathom Ahuva ALmogi-Labin G. Fridland-Wunder 《Phytoparasitica》1989,17(2):81-89
Adult populations ofMaladera matrida Argaman (Coleoptera: Scarabaeidae), a beetle highly injurious to agricultural crops, were studied in the coastal plain of Israel from 1985 to 1988. There were two generations annually. Adults emerged in March, April or May and disappeared in October or November. The beetles are on the wing, mate, and feed at twilight and in the dark. Feeding and mating behavior, sex ratio, and preferred food plants were studied. 相似文献
7.
8.
Two entomophthoraceous species,Zoophthora radicans andEntomophthora planchoniana, were found on the yellow pecan aphid,Monellia costalis, shortly after it was introduced into Israel. This aphid is a new host forZ. radicans anywhere. The fungus was isolated in pure culture and artificially inoculated on three Dipteran species:Musca domestica, Drosophila melanogaster andCeratitis capitata, as well as on the HemipteranEmpoasca sp. from pecan. The identity ofZ. radicans and some new characters,e.g. the production of two kinds of rhizoids, are discussed. The possibility of usingM. domestica as a vector for artificial spread of the fungus, as a means of biological control of various insect pests, is advocated. 相似文献
9.
Carmen García-Limones Ana Hervs Juan A. Navas-Corts Rafael M. Jimnez-Díaz Manuel Tena 《Physiological and Molecular Plant Pathology》2002,61(6):325
To ascertain if active oxygen species play a role in fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceris, the degree of lipid peroxidation (malondialdehyde formation) and the activity levels of diamine oxidase (DAO), an apoplastic H2O2-forming oxidase, and several antioxidant enzymes, namely ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol-dependent peroxidase (GPX) and superoxide dismutase (SOD), were determined spectrophotometrically in roots and stems of ‘WR315’ (resistant) and ‘JG62’ (susceptible) chickpea cultivars inoculated with the highly virulent race 5 of the pathogen. Moreover, APX, CAT, GPX and SOD were also analysed in roots and stems by gel electrophoresis and activity staining; and the protein levels of APX and SOD in roots were determined by Western blotting. In roots, infection by the pathogen increased lipid peroxidation and CAT and SOD activities, although such responses occurred earlier in the incompatible compared with the compatible interactions. APX, GPX and GR activities were also increased in infected roots, but only in the compatible interaction. In stems, infection by the pathogen increased lipid peroxidation and APX, CAT, SOD and GPX activities only in the compatible interaction, and DAO activity only in the incompatible one. In general, electrophoregrams agreed with the activity levels determined spectrophotometrically and did not reveal any differences in isoenzyme patterns between cultivars or between infected and non-infected plants. Further, Western blots revealed an increase in the root protein levels of APX in the compatible interaction and in those of SOD in both compatible and incompatible interactions. In conclusion, whereas enhanced DAO activity in stems, and earlier increases in lipid peroxidation and CAT and SOD activities in roots, can be associated with resistance to fusarium wilt in chickpea, the induction of the latter three parameters in roots and stems along with that of APX, GR (only in roots) and GPX (only in stems) activities are rather more associated with the establishment of the compatible interaction. 相似文献
10.
Marlene Cordero Pedro L. Ramos Lester Hernández Ana I. Fernández Ana L. Echemendía Rudy Peral Gloria González Daniel García Surey Valdés Ana Estévez Keren Hernández 《Phytoparasitica》2003,31(5):478-489
The presence of a begomovirus in potato plants with yellow mottle symptoms was determined for the first time in Cuba. The
incidence of typical begomovirus-like symptoms in potato plants in some regions of Havana province (Güira de Melena, San José
de las Lajas, Güines and Boyeros) during the growing seasons from 1992 to 1998 was in general low. However, in some cultivars
belonging to the National Program for Potato Genetic Improvement, the incidence reached 100%. Yield losses, determined in
1992 and 1994, ranged as high as 19% to 56.33% depending on the cultivar. Characterization of the causal agent was done by
light microscopy, host range (graft and mechanical transmission), DNA hybridizations, polymerase chain reaction, and restriction
fragment length polymorphism analysis. Nucleotide sequence of the amplified fragments revealed the presence ofTomato mottle Taino virus. The virus was transmittedvia tubers and has been detected in mixed infections withPotato virus X and withPotato leaf roll virus.
http://www.phytoparasitica.org posting Oct. 20, 2003. The first two authors contributed equally to this work. 相似文献