Validation,use, and disadvantages of enzyme-linked immunosorbent assay kits for detection of cortisol in channel catfish,largemouth bass,red pacu,and golden shiners     

Sink TD  Lochmann RT  Fecteau KA 《Fish physiology and biochemistry》2008,34(1):95-101

Measurement of cortisol response is an important tool to asses stress in fisheries research. Radioimmunoassay (RIA) is a common method for the measure of cortisol in fish. Use of enzyme-linked immunosorbent assays (ELISA) to detect cortisol would eliminate health hazards, costs of handling radioisotopes, and the short stability time associated with RIA. Enzyme-linked immunosorbent assays have been used for the determination of cortisol in several fish species. However, the ELISA method of cortisol determination in fish lacks proper validation testing. We conducted validation procedures for multiple commercial cortisol ELISA kits and compared the results to RIA. The assays were tested for four species: (1) channel catfish Ictalurus punctatus, (2) largemouth bass Micropterus salmoides, (3) red pacu Piaractus brachypomus, and (4) golden shiners Notemigonus crysoleucas. We evaluated the ELISA methods against RIA, and determined that at least one kit is suitable (accuracy: mean recovery of spiked samples, 102.8%; reproducibility: interassay coefficient of variation < 10.5% for all species; precision: intra-assay coefficient of variation < 16.8% for all species; linearity: R ² > 0.96 for all species) for the measurement of cortisol response in fish and comparative determination of stress. All of the ELISA assay results varied by more than 10% from the cortisol concentrations detected by the RIA. The high variability of the kit results indicates that commercial ELISA kits could be utilized for qualitative determination of cortisol in fish, but should be fully validated in each laboratory for each species before being used for research.  相似文献   

Determination of the potassium balances in diary cows and the examination of daily and lactation period-associated variations          下载免费PDF全文

Sattler N  Fecteau G  Couture Y  Tremblay A 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(2):107-115

The objectives of this study were to evaluate a method of determining the internal and external potassium balances in dairy cows and to determine daily and lactation period-associated variations of these balances. Ingested potassium, milk potassium concentration (LK) and potassium urinary fractional clearance (FeK) defined the potassium external balance. Serum (SK) and red blood cell (CIEK) potassium concentrations defined the potassium internal balance. Whole blood, urine, and milk were collected at different times of the day from 19 cows, grouped in 5 lactation periods. Significant differences were observed for LK between lactation periods. Significant variations occurred during the day for CIEK, LK, and FeK. Thus, in order to monitor potassium balances during a time period, samples should be collected at the same time each day. This method of determining both potassium balances simultaneously could be useful in the study of the bovine hypokalemia syndrome.  相似文献   

Failure of dogs to shed oocysts after being fed bovine fetuses naturally infected by Neospora caninum     

Bergeron N  Fecteau G  Villeneuve A  Girard C  Paré J 《Veterinary parasitology》2001,97(2):145-152

Neospora caninum is a protozoan that causes abortion in cattle. The dog has recently been identified as a definitive host for N. caninum. To verify if bovine fetuses can infect dogs, nine 2-4-month-old dogs were fed bovine fetuses naturally infected by N. caninum. None of the dogs excreted oocysts, seroconverted, had clinical signs or lesions compatible with N. caninum infection. Additional studies will be necessary to determine the natural mode of infection of dogs by N. caninum.  相似文献   

Virulence factors in Escherichia coli isolated from the blood of bacteremic neonatal calves     

Fecteau G  Fairbrother JM  Higgins R  Van Metre DC  Paré J  Smith BP  Holmberg CA  Jang S 《Veterinary microbiology》2001,78(3):241-249

Twenty-five Escherichia coli isolates from the blood of critically ill bacteremic calves sampled in two separate studies on a calf-rearing farm housing over 15,000 calves, in the San Joaquin Valley, California were studied.Isolates were characterized for O serogroups and for pathotypes as determined by the presence of specific virulence factors including heat-labile enterotoxin (LT), heat-stable enterotoxins a and b (STa, STb), verotoxins 1 and 2 (VT1, VT2), cytotoxic necrotizing factor (CNF), aerobactin, intimin Eae and P, F17 and CS31A fimbrial adhesins, and resistance to bactericidal effects of serum.These isolates constituted a heterogeneous group. However, isolates were mostly aerobactin positive and often resistant to the bactericidal effects of serum. Isolates of pathotypes O78 (n=6), O119:CS31a (n=3), and P positive but O non-typeable (n=3) were associated with a high mortality rate. The remaining isolates belonged to diverse pathotypes, often possessing the adhesins P, F17, CS31A and Eae but belonging to O serogroups other than O78 and O119, and were less frequently associated with mortality.Although no virulence factor common to all isolates was identified, the capacity to use iron by the presence of aerobactin which is important to the capture of iron was a predominant factor. Moreover, certain pathotypes appear to be associated with primary colisepticemia whereas other pathotypes may cause a bacteremia without necessarily leading to septicemia.  相似文献   

Control measures for contagious enteric diseases in a veterinary teaching hospital          下载免费PDF全文

Ravary B  Fecteau G  Higgins R  Paré J  Lavoie JP 《The Canadian veterinary journal. La revue veterinaire canadienne》1999,40(12):871-877

Instructions and control measures related to enteric contagious diseases at the Veterinary Medical Teaching Hospital (VMTH) of the Faculty of Veterinary Medicine of the University of Montreal are presented. These control measures, which have given satisfactory results within the past decade, are exemplified by a salmonellosis outbreak that occurred in spring 1996 in the large animal clinic of the VMTH. Emphasis was put on the importance of antigenic and/or genetic characterizations of Salmonella isolates, in order to detect an eventual source of contamination, but also to determine the incidence of nosocomial infections among hospitalized animals.  相似文献   

Health status and risk factors associated with failure of passive transfer of immunity in newborn beef calves in Québec          下载免费PDF全文

Filteau V  Bouchard E  Fecteau G  Dutil L  DuTremblay D 《The Canadian veterinary journal. La revue veterinaire canadienne》2003,44(11):907-913

Risk factors associated with failure of passive transfer of immunity (FPT) were evaluated among newborn beef calves in Québec. Physical examination was performed on calves born of a normal calving and blood samples were collected for determination of health status and measurement of serum concentration of immunoglobulin (Ig) G1. Of 225 calves, from 45 herds, 19% showed FPT (serum IgG1 concentration < 10.0 g/L). Calves born in a stanchion-stall were more likely to show FPT (OR: 10.2). Calves bottle-fed colostrum were less at risk for FPT (OR: 0.06). Calf gender, month of birth, dam parity, and dam body condition score were not associated with FPT. No association was detected between FPT and health status. Special care should be given to calves born from cows in a stanchion-stall to ensure adequate colostrum intake. Failure of passive transfer of immunity should be considered with other risk factors when investigating morbidity.  相似文献   

Mechanical ventilation of a cloned calf in respiratory failure   总被引：1，自引：0，他引：1  

Sébastien Buczinski  Dr. Vet  DACVIM    Søren R. Boysen  DVM  DACVECC    Gilles Fecteau  DMV  DACVIM 《Journal of Veterinary Emergency and Critical Care》2007,17(2):179-183

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Dysfibrinogenemia or afibrinogenemia in a Border Leicester lamb.          下载免费PDF全文

G Fecteau  J G Zinkl  B P Smith  S O'Neil  S Smith    S Klopfer 《The Canadian veterinary journal. La revue veterinaire canadienne》1997,38(7):443-444

Hereditary fibrinogen deficiency is a rare condition in all species. Measurement of plasma fibrinogen should indicate low levels. Specific factor assays and pedigree analysis are essential in establishing a definitive diagnosis of the hereditary deficiency. Differentiation between afibrinogenemia, hypofibrinogenemia, and dysfibrinogenemia requires sophisticated techniques and assistance from a specialized laboratory.  相似文献   

Endoscopic removal of esophageal and ruminal foreign bodies in 5 Holstein calves     

Diego E. Gomez  Nicola C. Cribb  Luis G. Arroyo  André Desrochers  Gilles Fecteau  Sylvain Nichols 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(10):965-969

Endoscopic removal of esophageal and ruminal foreign bodies was successfully performed in 5 Holstein-Friesian calves under sedation or general anesthesia by using an electrocautery snare or a wire-guided Dormi basket. This report describes the endoscopic manipulations, treatment, and outcomes of esophageal foreign body removal in these calves.  相似文献   

Letter to the Editor     

Dianne Mawby  Jacqueline Whittemore  Kellie Fecteau 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(6):1637-Dec;28(6):1637

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