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Didik T Subekti Fitrine Ekawasti Muhammad Ibrahim Desem Zul Azmi 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(6)
Toxoplasma gondii consists of three genotypes, namely genotype I, II and III. Based on its virulence, T. gondii can be divided into virulent and avirulent strains. This study intends to evaluate an alternative method for predicting T. gondii virulence using hierarchical cluster analysis based on complete coding sequences (CDS) of sag1, gra7 and rop18 genes. Dendrogram was constructed using UPGMA with a Kimura 80 nucleotide distance measurement. The results showed that the prediction errors of T. gondii virulence using sag1, gra7 and rop18 were 7.41%, 6.89% and 9.1%, respectively. Analysis based on CDS of gra7 and rop18 was able to differentiate avirulent strains into genotypes II and III, whereas sag1 failed to differentiate. 相似文献
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Fitrine EKAWASTI Kazuya KITAGAWA Hiroshi DOMAE April Hari WARDHANA Junki NAGASAWA Tomoyuki SHIBAHARA Masaharu TOKORO Kazumi SASAI Makoto MATSUBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1240
Oocysts of Isospora sp. were detected in the feces of a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) kept at a zoo in Ishikawa, Japan. Phylogenetic analysis placed the sequence in the cluster of Isospora spp. isolated from reptiles. Based on a comparison of morphological data of ten previously reported Isospora species from the Chamaeleonidae family, this isolate was morphologically similar to I. jaracimrmani, which has been considered to be a virulent species. This case study suggests the possibility that species of Isospora might not always cause disease because the animal that shed these oocysts showed no symptoms for more than two months. 相似文献
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