Pathology of genetically engineered mice, Ward JM, Maranpot JR, Sundberg JP, Frederickson RM     

DA Pass 《Australian veterinary journal》2001,79(3):198-198

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8 MEASUREMENT OF ARTICULAR CARTILAGE STIFFNESS IN THE FEMOROPATELLAR, TARSOCRURAL AND METATARSOPHALANGEAL JOINTS OF HORSES AS A PREDICTOR OF CARTILAGE PATHOLOGY.     

E  Garcia-Seco  DA Wilson  K Kuroki  J Cook  J Kreeger  KG Keegan 《Veterinary surgery : VS》2003,32(6):600-600

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绵羊卵泡发育过程中关键信号通路研究进展     

陈欣  达赖  付绍印  祁云霞  王标  何小龙  马跃军  方勤圆  张林  贾明蕊  董鹏  刘永斌 《中国畜牧兽医》2021,48(3):963-974

卵泡从原始卵泡发育为成熟卵泡，直至排卵、黄体发育等过程都受到精密的调控，产生大量的优势卵泡是绵羊产多羔及实现快速扩繁的关键因素。研究发现，相关信号通路和转录因子通过影响绵羊卵泡中卵母细胞、颗粒细胞的生长，进而调控卵泡的发育成熟，对这些信号通路进行深入了解，有助于探索卵泡发育的调控机制，早日实现绵羊高效繁育。Notch是卵泡发育过程中发挥重要作用的高度保守信号通路，PI3K/AKT/mTOR信号通路各成员都是广泛存在于细胞内的信号转导分子，在卵泡发育早期发挥了主要作用，还有间隙连接（gap junction，GJ）和跨带突触（transzonal projections，TZPs）等物理连接方式，在细胞间的交流通讯起到重要作用。作者详细介绍了Notch信号通路、PI3K/AKT/mTOR信号通路、间隙连接及跨带突触的结构功能在绵羊卵泡发育中的调控作用，为进一步探明绵羊卵泡发育的调控机制提供参考。  相似文献   

以全产业链思维优化甘肃省“粮改饲”生产力布局     

笪晓军  王兴琪  刘永康  马璐  王凤萍 《中国饲料》2021,1(6):143-146

用全产业链思维优化“粮改饲”生产力布局具有重要的现实意义和理论价值。本文以甘肃省为例,总结甘肃省“粮改饲”工作已取得的工作成效和不足,论述了“粮改饲”构建全产业链的必要性,初步探讨了甘肃省“粮改饲”全产业链模式和布局优化,并提出完善相关支持政策、培育壮大龙头企业、高度重视品牌建设和创新科技带动引领4条对策建议。 [关键词]全产业链|粮改饲|生产力布局|甘肃省  相似文献   

Seroprevalence of Babesia bigemina in smallholder dairy cattle in Tanzania and associated risk factors     

Swai ES  Karimuribo ED  French NP  Fitzpatrick JL  Bryant MJ  Kambarage DM  Ogden NH 《Journal of the South African Veterinary Association》2007,78(1):15-20

Variations in the seroprevalence of antibody to Babesia bigemina infection by farm and animal level risk factors were investigated for 2 contrasting regions of Tanga and Iringa in Tanzania. Tanga is situated in the eastern part of the country and has typical tropical coast climate while Iringa is situated in the Southern Highlands and has a tropical highland climate. Two hundred farms from each region were selected using simple random sampling procedure and visited once between January 1999 and April 1999. Blood samples were collected from 1329 smallholder dairy animals on selected farms for harvesting serum which was subsequently used for serodiagnosis of B. bigemina using an indirect enzyme linked immuno-sorbent assay (ELISA). Of the 1329 sera samples screened, 34.9 % were positive for B. bigemina. The prevalence was higher in Iringa Region [43%, 95% confidence intervals (CI) = 39.5-47.3] than in Tanga Region (27%, CI = 23.6-30.5). Using a logistic binomial regression model as an analytical method for predicting the likelihood of animal seropositivity, we found (in both regions) that the risk of positive reaction varied with the animal's age, history of grazing and geographical location. Seroprevalence increased with age (beta = 0.01 and 0.01 per year of age, P < 0.005 in Tanga and Iringa, respectively). Animals located in Lushoto and Iringa urban district were associated with increased risk of seropositivity [Odds ratio (OR) = 4.24, P = 0.001, for Lushoto, and OR = 1.81, P = 0.040, for Iringa Urban, respectively). Animals grazed 3 months prior to sampling had higher odds for seropositivity than zero/semi-grazed, despite farmer-reported high frequency of tick control (OR = 2.71, P = 0.0087, for Tanga, and OR = 4.53, P = 0.001, for Iringa). Our study suggests that even though herd sizes are small, B. bigemina infection is widespread in many smallholder dairy farms and endemic stability with respect to this disease has not yet been attained, but the observed levels are sufficiently high to ensure that clinical disease would be a risk.  相似文献   

Approaches to pathogen-mediated resistance breeding against plum pox potyvirus in stone-fruit trees1     

A. DA CMARA MACHADO  E. KNAPP  H. PÜHRINGER  G. SEIFERT  V. HANZER  H. WEISS  H. KATINGER  M. LAIMER DA CMARA MACHADO 《EPPO Bulletin》1994,24(3):697-704

In a programme for developing systems which allow the transfer of foreign genes into apricot cultivars, we have tested cotyledons of immature embryos, somatic embryos and leaf discs. Apricot plants have been transformed, and then regenerated, with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids: pBinGUSint, carrying the marker gene β-glucuronidase (GUS), and pBinPPVm, carrying the coat-protein gene of plum pox potyvirus (PPV). The marker gene GUS was used for visual evaluation of the efficiency of the transformation system. The coat-protein gene was used in the hope of introducing coat protein-mediated resistance to one of the most important stone-fruit pathogens in Europe and the Mediterranean area.  相似文献   

Observations on ascites in nine cattle     

Milne MH  Mellor DJ  Barrett DC  Fitzpatrick JL 《The Veterinary record》2001,148(11):341-344

Signs of ascites were observed in only nine of 1863 cattle examined over a period of five years. The ascites was most commonly associated with either primary or secondary cardiac disease; cattle with ascites have a poor prognosis because the condition is usually assocaited with terminal disease. Two animals had cor pulmonale with chronic pneumonia, three had cardiomyopathy, one had bacterial endocarditis, two had thrombosis of the caudal vena cava and one had diffuse abdominal epithelioid mesothelioma.  相似文献   

Use of clinical information to predict the characteristics of bacteria isolated from clinical cases of bovine mastitis     

Milne MH  Biggs AM  Fitzpatrick JL  Innocent GT  Barrett DC 《The Veterinary record》2003,152(20):615-617

Farmers recorded the clinical signs of cows with clinical mastitis and submitted milk samples for bacteriological examination, so that the clinical signs could be correlated with the bacteriological findings. Odds ratios for the demeanour of the cow, the appearance of the milk, milk yield, udder texture, and the administration of parenteral antibiotics were calculated for mastitis cases classified in terms of their microbiology as either enterobacteriaceae, major Gram-positive pathogens, minor pathogens, 'no growths' or 'all other pathogens'. Animals infected with enterobacteriaceae had the highest odds of being reported as having a reduced milk yield, swollen or hard udders, watery milk and/or being systemically sick. A logistic regression model was used to predict the Gram-staining characteristics of the bacteria causing clinical mastitis. The clinical findings found to be significant predictors in the model were the demeanour of the cow and its milk yield. The regression model was used as a basis for a predictive test. Using a test data set, the sensitivity of the test was 28 per cent, its specificity was 96 per cent, the positive predictive value was 74 per cent and the negative predictive value was 80 per cent. The overall accuracy of these predictions was 79 per cent.  相似文献   

Survey of the views of graduates (1993 to 1997) on the undergraduate veterinary clinical curriculum in the British Isles     

Fitzpatrick JL  Mellor DJ 《The Veterinary record》2003,153(13):393-396

In 1998 a questionnaire was sent to graduates from all the veterinary schools in Great Britain and Ireland who had obtained their veterinary degree within the previous five years, to assess their opinions of the undergraduate clinical veterinary curriculum. Ninety-five per cent of the graduates who responded were working full time in veterinary practice, with small animal work occupying 90 per cent of them for a median of 70 per cent of their time. Their assessment of the curriculum suggested that they were generally satisfied, but that there were some subjects they considered important in which the teaching and extramural studies had failed to provide adequate learning opportunities. Twelve subjects were rated as 'very important', two subjects, small animal medicine and anaesthesia, were considered to be 'very well' taught, and extramural studies were considered to be 'very useful' for three subjects, small animal surgery, cattle medicine and cattle surgery. The survey provided evidence that graduates are keen to continue learning and specialise after they graduate.  相似文献   

Evaluation of PCR to detect Theileria parva in field-collected tick and bovine samples in Tanzania     

Ogden NH  Gwakisa P  Swai E  French NP  Fitzpatrick J  Kambarage D  Bryant M 《Veterinary parasitology》2003,112(3):177-183

The ability of PCR to detect infections of Theileria parva, the cause of East Coast Fever, in field-collected tick and bovine samples from Tanzania was evaluated. PCR-detected infection prevalence was high (15/20, 75%) in unfed adult Rhipicephalus appendiculatus ticks that fed as nymphs on an acutely-infected calf, but low (22/836, 2.6%) in unfed adult R. appendiculatus collected from field sites in Tanzania. Tick infection prevalence was comparable to that in previous studies that used salivary gland staining to detect T. parva infection in field-collected host-seeking ticks. Of 282 naturally-exposed zebu calves, seven had PCR-positive buffy coat samples prior to detection of Theileria spp. parasites in stained buffy coat cells or lymph node biopsies. Evidence of Theileria spp. infections was detected in stained smears of lymph node biopsies from 109 calves (38.6%) and buffy coat samples from 81 (28.7%), while buffy coat samples from 66 (23.4%) were PCR-positive for T. parva. Implications of these findings for the sensitivity and specificity of the PCR are discussed.  相似文献