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1.
Youssef A. Attia Fulvia Bovera Abd El-Hamid E. Abd‐Elhamid Serena Calabr Mohamed A. Mandour Mohammed A. Al‐Harthi Sabar S. Hassan 《Journal of animal physiology and animal nutrition》2019,103(3):947-958
Sixty‐four nulliparous female rabbits were distributed among eight groups (eight animals/group). Group one was the unsupplemented control group; the other seven groups were supplemented with zinc bacitracin (ZnB) at 100 mg, or bee pollen (BP) and/or propolis (Pro) at 150 and 300 mg in a capsulated form, three times a week, day after day, continuously all over the experimental period. The experiment was run for eight parties; at each parity, 28 kids of each doe group (a total of 224 rabbits) were divided into two subgroups weaned, respectively, at 24 and 30 days of age. Thus, for each parity, there were 16 groups (eight does treatments × two weaning age, 14 rabbits per group). The growing rabbits fed the standard diets without supplements. The growth performance, the carcass traits, the liver and the spleen histology of rabbits were checked up to 90 days of age to find possible carryover effects of the supplements. The supplements had no significant effect on most of the growth performance at 90 days of age, but BP150 and BP+Pro300 increased the growth rate in comparison with ZnB group. The liver weight in the control, BP300 and Pro300 groups was higher than the ZnB one. The spleen weight was higher in the groups ZnB, BP150, Pro300 and BP+Pro300, followed by the control, BP300 and BP+Pro150 and thus Pro150. The heart % in the BP150 and Pro300 groups was higher than ZnB and BP+Pro150 groups. A lymphoid hyperplasia of splenic white pulp was observed in the BP+Pro groups, while propolis alone showed a mild activation of lymphobiosis. The Pro and BP groups showed the same picture of the control group exhibiting a hydropic degeneration of mostly hepatic cells, while the ZnB group exhibited adverse effect on the bile ducts featuring portal periductal inflammatory cells infiltration with epithelial hyperplasia reflecting chronic cholangitis. 相似文献
2.
Youssef A. Attia Fulvia Bovera Abd El‐Hamid Abd Elhamid Sameer A. Nagadi Mohamed A. Mandour Saber Sh. Hassan 《Journal of animal physiology and animal nutrition》2019,103(3):959-968
To evaluate the effect of bee pollen (BP) and/or propolis (Pro) supplementation on rabbit does, 64 nulliparous NZW rabbits does were distributed among eight groups (eight animals/group). One unsupplemented group was the control; the other seven groups were supplemented, respectively, with zinc bacitracin (ZnB) at 100 mg, BP at 150 and 300 mg, Pro at 150 and 300 mg, BP+Pro at 150 and 300 mg of each three times/week, day after day continuously along eight parities. The BP300, Pro300 and BP+Pro150 groups had higher body weight of litter at birth and number of kids born alive. The BP supplementation at 150 mg increased plasma total protein and albumin than the control group. The BP or Pro at 150 mg decreased plasma T3 than the other groups except for BP+Pro150. The ZnB group had significantly greater T3/T4 ratio compared to BP, Pro and BP+Pro at 150 mg. The BP+Pro150 group had less ALT than the control; BP300 and Pro 300 mg resulted in lower plasma AST than the groups Pro150 with or without BP and the control group. The plasma alkaline phosphatase of BP at 150 or 300 mg and BP+Pro150 was significantly greater than that of the Pro150 group. The BP+Pro300 group had higher WBCs than the other groups. In contrast, the lymphocytes were greater in the Pro and BP+Pro300 groups than in BP, Pro and BP+Pro at 150 mg. The groups supplemented with BP and BP+Pro at 150 and 300 mg had significantly greater SRBCs of doe rabbits and their offspring compared to the control and the ZnB group. The BP at 300 mg increased the serum albumin and α1‐globulin than the control group. The Pro300 group had greater serum α2‐globulin and β‐globulin than the control group. The total globulin was significantly greater for the 300 mg propolis‐supplemented groups than the control. 相似文献
3.
Giovanni Piccolo Stefania Marono Fulvia Bovera Raffaella Tudisco Gaetano Caricato Antonino Nizza 《Aquaculture Research》2008,39(16):1697-1704
Two hundred and fifty soles (30 g initial weight) were randomly stocked in 12 tanks (recirculation system) at two different stocking densities (2.3 and 1.3 kg m?2) and fed on two different diets (50% and 54% of crude protein and 21% and 18% of total lipid, respectively, for diets A and B) with triplicate tanks for treatment. The trial lasted for 300 days. Fish stocked at low density showed a significantly higher weight gain than the fish reared at 2.3 kg m?2 density (94.1±7.4 vs. 78.9±8.2 g, P<0.01) with a specific growth rate (SGR) of 0.46±0.11 and 0.43±0.16 (P<0.01) respectively. Feed conversion ratio (FCR) was more favourable for lower density groups (2.50 vs. 2.64, P<0.01). Diet B led to a higher final weight (124.1 vs. 110.2 g, P<0.01) and a better feed utilization (FCR: 2.49 vs. 2.65, P<0.01). Except for the lipid content, which was higher in the low‐density group (2.6% vs. 2.3%, P<0.05), proximate composition of sole's muscle was not influenced by treatments. Dover sole reared at low stocking density showed higher monounsaturated fatty acid (35.27% vs. 34.40%, P<0.01) and lower saturated fatty acid (24.36 vs. 26.13%, P<0.01) contents, and lower atherogenic (0.67 vs. 0.75, P<0.01) and thrombogenic (0.28 vs. 0.32, P<0.01) indexes. 相似文献
4.
Das M Drake T Wiley DJ Buchwald P Vavylonis D Verde F 《Science (New York, N.Y.)》2012,337(6091):239-243
Cells promote polarized growth by activation of Rho-family protein Cdc42 at the cell membrane. We combined experiments and modeling to study bipolar growth initiation in fission yeast. Concentrations of a fluorescent marker for active Cdc42, Cdc42 protein, Cdc42-activator Scd1, and scaffold protein Scd2 exhibited anticorrelated fluctuations and oscillations with a 5-minute average period at polarized cell tips. These dynamics indicate competition for active Cdc42 or its regulators and the presence of positive and delayed negative feedbacks. Cdc42 oscillations and spatial distribution were sensitive to the amounts of Cdc42-activator Gef1 and to the activity of Cdc42-dependent kinase Pak1, a negative regulator. Feedbacks regulating Cdc42 oscillations and spatial self-organization appear to provide a flexible mechanism for fission yeast cells to explore polarization states and to control their morphology. 相似文献
5.
Barbara Scaglia Fulvia Tambone Pier Luigi Genevini Fabrizio Adani 《Compost science & utilization》2013,21(2):90-98
Forty-five organic matrices of different types and origin were subjected to the test for the determination of biological stability by means of dynamic respirometric measurement (DRI) and static respirometric measurement (SRI). The results obtained from the comparison between the two indexes indicated that the SRI, compared with the DRI, underestimates the consumption of oxygen by the biomasses and consequently their rate of biological stability by a factor equal to 2. From the analysis of the data it is possible to define the biological stability as DRI<1000 mgO2kg?1VSh?1. The DRI data were correlated with the temperatures of the biomasses showing a linear correlation (R2=0.81, p<0.01) only for stable matrices while for nonstable matrices no significant correlation is found. 相似文献
6.
Raffaella Tudisco Federico Infascelli Monica Isabella Cutrignelli Fulvia Bovera Caterina Morcia Primetta Faccioli Valeria Terzi 《Livestock Science》2006,105(1-3):12-18
The effect of the digestion process in the gastro-intestinal tract (GIT) of animal models on the fate and integrity of plant DNA has been widely evaluated since DNA availability and integrity is a key factor for hypothetical horizontal gene transfer of recombinant DNA from GM crop-derived feeds to animal and human gut microflora. In this study, plant DNA sequences from high and low copy number genes were monitored in GIT and tissues of buffaloes and rabbits. Using a real-time PCR approach to track plant DNA in animal samples, we demonstrated the persistence of fragmented plant DNA blood and tissues of buffaloes and rabbits raised with conventional feeding. 相似文献
7.
Rita Redaelli Paolo Laganà Fulvia Rizza Orazio Li Destri Nicosia Luigi Cattivelli 《Euphytica》2008,164(3):679-687
The genetic gain of oats in Italy over the past 40 years was evaluated using 14 cultivars belonging to three different groups:
(i) five cultivars derived from local populations at the beginning of the oat breeding in Italy; (ii) four foreign cultivars,
introduced in Italy in the 1980s; (iii) five modern cultivars, selected through specific breeding programmes. The genotypes
were tested for 2 years in three locations characterised by different climatic conditions and agricultural practices; furthermore
in one location in Southern Italy, irrigated and rainfed plots were compared. Genetic gain was estimated equal to 3.6 g m−2 y−1 based on eight environments. The performance of the most recent cultivars was high under both irrigated and rainfed conditions,
showing a good adaptation of these genotypes to both fertile and drought prone environments. Finally, yield increase in oats
was found to be negatively correlated with plant height and positively correlated with test weight and seed weight. 相似文献
8.
Gussoni M Greco F Vezzoli A Paleari MA Moretti VM Beretta G Caprino F Lanza B Zetta L 《Journal of agricultural and food chemistry》2006,54(18):6725-6732
The effects of storage at 4 degrees C on the quantity and quality of chemical components in the caviar from farmed Acipenser transmontanus have been analyzed by SEM, chemical methods, and NMR and MRI techniques. Particular attention has been focused on the lipid components, the distribution and mobility of which were strongly affected by the storage time. MRI and relaxation data indicated that lipids are endowed with two different mobility regimes, one slow (short T1) and one fast (long T1), both lengthening with the storage time. Chemical analysis assessed a total fat content that remained practically unchanged and a constant fatty acid composition during the total storage time. The combination of the two methods allowed one (a) to suppose that a mechanism of lipid hydrolysis (faster in unsalted than in salted eggs) is still occurring during storage of caviar at 4 degrees C for up to approximately 4 months and (b) to exclude that an intensive oxidative process is active in the same storage period. 相似文献
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10.
Reina R Juganaru MM Profiti M Cascio P Cerruti F Bertolotti L De Meneghi D Amorena B Rosati S 《Veterinary immunology and immunopathology》2011,139(2-4):237-244
Genotype E of small ruminant lentivirus has been recently described in goats from different breeds in Italy. Genotype E infection may differ from known genotypes since deletions of dUTPase and VPR proteins have been confirmed in different independent areas and goat breed, and play a key role on virus replication and pathogenesis. In particular, genotype E Roccaverano strain has been described as low pathogenic since does not lead to clinical symptoms in goats. In contrast, classical CAEV infected goats of the same area and breed presented arthritis. In this study, we have used intratracheal and intra-bone marrow routes to establish genotype E persistent infections. Humoral and cellular immune responses elicited in the host against genotype E and genotype B derived antigens were evaluated until 200 days post-inoculation. Compared to genotype B antigen, seroconversion against genotype E GAG P16-25 antigen was detected at 2-3 weeks after inoculation, significantly earlier and at higher titres. Interestingly, antibody avidity did not increase in the course of the experiment neither against P16-25 nor against SU5, both derived from genotype E. T cell proliferation against P25-GST fusion protein antigens derived from genotype E was firstly detected at 15 days post-inoculation and was maintained throughout time until week 20 post-infection, while T cell proliferation against the genotype B P25 was not produced by the end of the experiment at 20 weeks post-inoculation. The strength of reaction was also higher when using P25 E as stimulator antigen. In contrast with antibody and T cell proliferation, cytotoxic-T-lymphocyte (CTL) activity in the circulating lymphocytes (effector cells) using blood-derived macrophages (BDM) as target cells, was not strain specific being surprisingly higher against genotype B infected antigen presenting cells (APCs). This is the first study reporting experimentally induced immunological changes in SRLV genotype E infection and indicates that CTL activity may be the adaptive immune response able to induce protection against heterologous infection. 相似文献