Potential of sexual reproduction among host-adapted populations of Phytophthora infestans sensu lato in Ecuador     

R. F. Oliva  L. J. Erselius  N. E. Adler  G. A. Forbes † 《Plant pathology》2002,51(6):710-719

To determine the potential of sexual reproduction among host-adapted populations of Phytophthora infestans sensu lato in Ecuador, 13 A1 isolates belonging to clonal lineages US-1, EC-1 and EC-3, and 11 A2 isolates belonging to the clonal lineage EC-2, were paired on agar plates to induce crossing. In the first experiment, six A1 isolates (three US-1, two EC-1 and one EC-3) were each crossed with three A2 isolates (total = 18 crosses). Matings involving isolates of the EC-1 lineage produced more oospores of healthy appearance than did matings with isolates of US-1 or EC-3. In the second experiment, the oospores of 35 crosses (21 EC-1 × EC-2; 10 US-1 × EC-2; four EC-3 × EC-2) were dispersed on water agar to assess oospore germination. Overall, germination percentages were low. Only one cross produced enough progeny for evaluation. Twenty-three single-oospore offspring were isolated and evaluated for mating type; electrophoretic patterns of glucose-6-phosphate isomerase ( Gpi ) and peptidase ( Pep ) alloenzyme loci; mitochondrial DNA haplotype; and genomic DNA fingerprint. Multilocus genotype data indicated that all 23 isolates resulted from meiotic recombination. Four progeny with homothallic phenotype appeared to be unstable heterokaryons. Markers at several loci segregated according to simple Mendelian expectations for a diploid organism, but the ratios of three RFLP loci and the Pep locus were not consistent with Mendelian expectations. All progeny were nonpathogenic on hosts of the parental genotypes. Reduced mating success and reduced pathogenic fitness of progeny appear to be postmating mechanisms of reproductive isolation in populations of P. infestans sensu lato in Ecuador.  相似文献   

Molecular Identification of a New Member of the Clover Proliferation Phytoplasma Group (16SrVI) Associated with Centaurea Solstitialis Virescence in Italy     

Francesco Faggioli  Graziella Pasquini  Valentina Lumia  Gaetano Campobasso  Timothy L. Widmer  Paul C. Quimby Jr 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(4):353-360

In the United States, yellow starthistle (Centaurea solstitialis) is an annual invasive weed with Mediterranean origins. Malformed plants displaying witches' broom, fasciations, abortion of buds and flower virescence symptoms were observed in central Italy. Attempts to transmit the causal agent from the natural yellow starthistle host to periwinkle by grafting, resulted in typical symptoms of a phytoplasma, i.e. yellowing and shortening of internodes. The detection of phytoplasmas was obtained from both symptomatic yellow starthistle and periwinkle by the specific amplification of their 16S-23S rRNA genes. PCR amplification of extracted DNA from symptomatic plant samples gave a product of expected size. Asymptomatic plants did not give positive results. An amplicon obtained by direct PCR with universal primers P1/P7 was cloned and sequenced. The homology search using CLUSTALW program showed more than 99% similarity with Illinois elm yellows (ILEY) phytoplasma from Illinois (United States) and 97% with Brinjal little leaf (BLL) phytoplasma from India. Digestion of the nested-PCR products with restriction enzymes led to restriction fragment length polymorphism patterns referable to those described for phytoplasmas belonging to the clover proliferation (16S-VI) group. Since this is a previously undescribed disease, the name Centaurea solstitialis virescence has been tentatively assigned to it. This is a new phytoplasma with closest relationships to ILEY and BLL, but distinguishable from them on the basis of 16S rDNA homology, the different associated plant hosts and their geographical origin.  相似文献   

Productive and biochemical responses of durum wheat to UV filtration     

Daniele Grifoni  Marco Napoli  Francesco Sabatini  Simone Orlandini  Marco Mancini  Gaetano Zipoli  Anna Dalla Marta 《Journal of Agronomy and Crop Science》2019,205(4):422-432

A UV exclusion experiment was conducted on durum wheat (Triticum durum Desf. var. Claudio) grown in pots. Plants were grown under three different radiative treatments in greenhouses covered with plastic filters: Teflon, transparent to the entire region of natural UV‐visible sunlight (TEF); polyester, transparent above 312 nm (MYL, excluding UVB) and Lee, transparent above 400 nm (LEE, excluding both UVA and UVB). Analyses have been carried out to determine the concentration of photosynthetic pigments in leaves, UV‐absorbing compounds, nitrogen and carbon in leaves, culms and spikes and proteins and gluten in grains. In particular, plants grown under UV exclusion showed a reduction in protein and dry gluten content (consequently without variation in the ratio dry gluten/protein), but at the same time, a significant increase of gluten index, which is a parameter to define the quality of gluten, was observed. The results highlighted the influence played by UV radiation on some biochemical parameters, mainly UV‐absorbing compounds, leaf nitrogen and grain protein characteristics of durum wheat cultivated under Mediterranean conditions. In particular, natural level of UV in the Mediterranean improves the characteristics of durum wheat flour important for pasta production (high dry gluten level), while the UV exclusion could improve characteristics of flour important for bread production (high gluten index).  相似文献   

FEASIBILITY FOR USING HYPOFRACTIONATED STEREOTACTIC VOLUMETRIC MODULATED ARC RADIOTHERAPY (VMAT) WITH ADAPTIVE PLANNING FOR TREATMENT OF THYMOMA IN RABBITS: 15 CASES          下载免费PDF全文

Mario Dolera  Luca Malfassi  Giovanni Mazza  Gaetano Urso  Massimo Sala  Silvia Marcarini  Nancy Carrara  Simone Pavesi  Sara Finesso  Michael S. Kent 《Veterinary radiology & ultrasound》2016,57(3):313-320

Thymoma is a relatively common tumor in rabbits. Treatment with surgery, radiation therapy, and chemotherapy alone or in combination has been reported with varying outcomes. Stereotactic volumetric modulated arc radiotherapy delivered in a hypofractionated manner allows high doses of radiation to be delivered to the target volume while allowing sparing of adjacent critical structures. This therapy is ideally suited for thymomas in rabbits given their size, the difficulty of multiple anesthesia episodes and the complexity of the radiotherapy plans required due to the tumor's proximity to the heart, lungs, and mediastinal structures. Fifteen rabbits with thymoma were prospectively recruited for this observational, single institution, single arm clinical study. All rabbits were imaged with both computed tomography (CT) and magnetic resonance imaging (MRI). A total dose of 40 Gy in six fractions was delivered using a single arc over an 11‐day period with repeat CT simulation done every other fraction for adaptive planning. Follow‐up evaluation was done through repeat CT and MRI imaging and revealed complete responses using the Response Evaluation Criteria in Solid Tumors (RECIST) criteria. Two rabbits had died at 618 and 718 days, 10 were alive and three were lost to follow‐up. Observed acute and late effects were graded according to the Veterinary Radiation Therapy Oncology Group (VRTOG) criteria and were found to be minimal.  相似文献   

Growth,Feed Utilization,and Glycemic Response in European Sea Bass,Dicentrarchus labrax,Juveniles Fed Carbohydrate of Different Complexities     

Paula Enes  Helena Peres  Ivone Almeida  Ana Couto  Aires Oliva‐Teles 《Journal of the World Aquaculture Society》2011,42(6):873-879

The effect of dietary carbohydrate complexity on growth, feed utilization, and glycemia was studied in European sea bass juveniles. Four isonitrogenous (50% crude protein) and isolipidic (15% crude lipids) diets were formulated to contain 20% pregelatinized maize starch (PGS diet), dextrin (DEX diet), maltose (MAL diet), or glucose (GLU diet). No effect of dietary carbohydrate complexity on growth was noticed. Feed efficiency and protein efficiency ratio were lower in fish fed the GLU diet than in the other groups, whereas the opposite was observed for feed intake. Plasma glucose peaked 3–4 h after feeding in fish fed the MAL and GLU diets, whereas in fish fed the PGS and DEX diets the peak was reached 5–6 h after feeding. Peak plasma glucose concentration (13 mmol/L) was higher in fish fed the GLU diet than the other diets (9 mmol/L). Shorter hyperglycemia duration was observed in fish fed the MALT and GLU diets (6 h) than the PGS and DEX diets (10 h). Complex carbohydrates delayed plasma glucose concentration peak compared with simple sugars, whereas the opposite was observed for hyperglycemia duration. Overall, dietary maltose, dextrin, and starch were apparently better utilized as energy source than glucose by European sea bass juveniles.  相似文献   

FOAM, a new simple benthic degradative module for the LAMP3D model: an application to a Mediterranean fish farm     

Patrizia De Gaetano  rea M Doglioli  Marcello G Magaldi  Paolo Vassallo  & Mauro Fabiano 《Aquaculture Research》2008,39(11):1229-1242

The modelling framework already introduced by Doglioli, Magaldi, Vezzulli and Tucci to predict the potential impact of a marine fish farm is improved following different directions, namely (1) real historic current-metre data are used to force the simulations, (2) settling velocity values specifically targeting Mediterranean fish species are used, and (3) a new benthic degradative module, the Finite Organic Accumulation Module, is added to the modelling framework. The Finite Organic Accumulation Module uses the output of the other functional units of the modelling framework to calculate the organic load on the seabed. The Finite Organic Accumulation Module considers the natural capability of the seafloor in absorbing part of the organic load. Different remineralization rates reflect the sediment stress level according to the work of Findlay and Watling. Organic degradation for both uneaten feed and faeces is evaluated by changing the release modality (continuous and periodical) and by varying the settling velocities. It is found that the maximum impact on the benthic community is observed either for quickly sinking uneaten feed released twice a day, or for less intense near-bottom current conditions. If both the above-mentioned scenarios coexist, a high stress level is established in the sediment. The model also suggests that the use of self-feeders in cages can reduce farm impacts significantly. These results show how the new and more complete modelling framework presented here is able to improve the objectivity in the decision-making processes and how it may be successfully used for planning and monitoring purposes.  相似文献   

Regulation of growth, fatty acid composition and delta 6 desaturase expression by dietary lipids in gilthead seabream larvae (Sparus aurata)     

Izquierdo MS  Robaina L  Juárez-Carrillo E  Oliva V  Hernández-Cruz CM  Afonso JM 《Fish physiology and biochemistry》2008,34(2):117-127

The Δ6 and Δ5 desaturases and elongases show only very limited activity in marine fish, and little is known of the possibility of enhancing Δ6 desaturase gene expression in these fish. The use of plant oils in marine fish diets is limited by their lack of n−3 highly unsaturated fatty acids (HUFA) despite an abundant content of the 18C fatty acid precursor linoleic and α-linolenic acids. The objective of the present study was to determine the ability of larval gilthead seabream to utilize vegetable oils and assess the nutritional regulation of Δ6 desaturase gene expression. Seventeen-day-old gilthead seabream larvae were fed during a 17-day period with one of four different microdiets formulated with either sardine fish oil (FO), soybean, rapeseed or linseed oils, respectively, or a fifth diet containing defatted squid meal and linseed oil. Good larval survival and growth, both in terms of total length and body weight, were obtained by feeding the larvae either rapeseed, soybean or linseed oils. The presence of vegetable oils in the diet increased the levels of 20:2n−9 and 20:2n−6, 18:2n−9, 18:3n−6, 20:3n−6 and 20:4n−6, in larvae fed rapeseed and soybean oils in comparison to those fed FO. In addition, a sixfold increase in the relative expression of Δ6 desaturase-like gene was found in larvae fed rapeseed and soybean oils, denoting the nutritional regulation of desaturase activity through its gene expression in this fish species. However, feeding linseed oil did not increase the expression of the Δ6 desaturase gene to such a high extent.  相似文献   

Fungicide dissipation curves in winemaking processes with and without maceration step     

Fernández MJ  Oliva J  Barba A  Cámara MA 《Journal of agricultural and food chemistry》2005,53(3):804-811

The evolution of residual levels of four fungicides (cyprodinil, fludioxonil, pyrimethanil, and quinoxyfen) during the elaboration of three types of wine with maceration (traditional red wine, carbonic maceration red wine, and red wine of long maceration and prefermentation at low temperature) and two types of wine without maceration (rose and white) has been studied. The disappearance curves of each fungicide have been analyzed during the period of each winemaking process (21 days) and during the different enological steps involved in the elaborations. The residual levels of fludioxonil reduce most quickly during the winemaking processes without maceration, whereas the decrease in levels of pyrimethanil was the slowest in practically all cases (with and without maceration). During carbonic maceration winemaking, the decay constant of cyprodinil was greater than that of the other pesticides in all assays (time and steps).  相似文献   

Accuracy of the Rotfinder instrument in detecting decay on Norway spruce (Picea abies) trees     

Jonàs Oliva  Carmen RomeraloJan Stenlid 《Forest Ecology and Management》2011,262(8):1378-1386

Rotfinder is a non-destructive decay-sensing apparatus based on resistance measurements in standing trees. The accuracy of Rotfinder in detecting decay was evaluated in 500 standing trees in three Norway spruce (Picea abies) plots. Trees were measured at three heights, 0.30, 0.66 and 1.30 m. Sections were later inspected for the presence of decay and reaction zones. Inspected trees were mostly infected by Heterobasidion annosum and showed a large variation in the amount of decay present, ranging from 0.1% to 88.0% of the section. Correctly and incorrectly classified trees were compared in terms of ion and element concentration, density and moisture. Measurements at stump level (0.30 m) were more accurate than measurements at breast height (1.30 m) where the reaction zone and decay columns showed lower moisture content. The accuracy of Rotfinder increased when trees with small decay columns were regarded as ‘non-decayed’. When only trees with more than 15% of the section decayed were regarded as ‘decayed’, Rotfinder had an accuracy of 0.86 when performing assessments at stump level. False negatives, as opposed to true positives, corresponded to trees with smaller and drier decay columns, drier reaction zones and lower K⁺ (potassium) concentration in the decay column. False positives corresponded to trees with large sapwood and high sodium content in the sapwood. Rotfinder represents an alternative to the standard method of using increment core observations to assess decay in living trees.  相似文献   

Evolution of chlorpyrifos, fenarimol, metalaxyl, penconazole, and vinclozolin in red wines elaborated by carbonic maceration of Monastrell grapes     

Navarro S  Oliva J  Barba A  Navarro G  Garcia MA  Zamorano M 《Journal of agricultural and food chemistry》2000,48(8):3537-3541

The influence of the different steps involved in the wine-making process on the disappearance of chlorpyrifos, fenarimol, metalaxyl, penconazole, and vinclozolin in red wines elaborated by carbonic maceration of Monastrell grapes was studied. The initial levels of the residues in grapes ranged from 0.28 mg/kg (penconazole) to 1 mg/kg (chlorpyrifos). Ten days after the beginning of maceration, the compound that had decreased least was chlorpyrifos (83% of initial value remaining), whereas metalaxyl, the least persistent of the residues, had decreased to 49% of its initial value. In the free-run juice, on the other hand, the highest percentage remaining corresponded to metalaxyl (10%) and the lowest to chlorpyrifos (0. 1%). After pressing, the percentages of initial values eliminated in pomace varied from 82.7% for chlorpyrifos to 17.7% for metalaxyl, whereas in the press juice the opposite was the case (37% metalaxyl and 2% chlorpyrifos remaining). In finished wine, there were residues of all the pesticides, with the exception of chlorpyrifos, metalaxyl (21%) being the most persistent. The percentages eliminated in the lees varied from 1.5 to 2.5% of the initial value.  相似文献