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Badaracco A Garaicoechea L Rodríguez D Uriarte EL Odeón A Bilbao G Galarza R Abdala A Fernandez F Parreño V 《Veterinary microbiology》2012,158(3-4):394-399
Bovine Group A Rotavirus (RVA) is one of the main causes of neonatal calf diarrhea worldwide. The present study reports the genotyping of bovine RVA strains circulating in Argentinean cattle from 2004 to 2010. Additionally, a new set of typing primers was designed and tested to differentiate between G8 and G6 (lineage III and IV) RVA strains. Bovine RVA was detected in 30% (435/1462) of the tested samples, corresponding to 49% (207/423) of the studied outbreaks with a similar detection rates in beef (53%; 67/127) and dairy herds (52%; 65/126). The RVA strains circulating in Argentinean cattle belonged to the common bovine genotypes G6 (lineages III and IV), G8, G10, P[5] and P[11]. A different RVA G/P-genotype distribution was found between the exploitation types, with the combination G6(IV)P[5] being by fare the most prevalent RVA strain in beef herds (58%), whereas a more even distribution of G6(III)P[11] (15%), G10P[11] (17%), G6(IV)P[5] (14%), and G6(IV)P[11] (6%) RVA strains was detected in dairy herds. G8 RVA strains were found in two dairy farms in calves co-infected with G8+G6(III)P[11]. A high percentage of co-infections and co-circulation of RVA strains with different genotypes during the same outbreak were registered in both exploitation types (20% of the outbreaks from beef herds and 23% from dairy herds), indicating a potential environment for reassortment. This finding is significant because G10P[11] and G6(III)P[11] strains may possess zoonotic potential. Continuous surveillance of the RVA strains circulating in livestock provides valuable information for a better understanding of rotavirus ecology and epidemiology. 相似文献
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Diego A. Galarza Antonio López-Sebastián Julián Santiago-Moreno 《Reproduction in domestic animals》2020,55(7):805-813
This study examines the effect of L-carnitine (LC) on chilled ram semen stored for up to 96 hr. Semen samples were collected, placed in a skimmed milk + 6% egg yolk extender, pooled, aliquoted and diluted with the same extender supplemented with different LC concentration: 0 (control), 1 mM (LC1), 2.5 mM (LC2.5), 5 mM (LC5), 7.5 mM (LC7.5) or10 mM (LC10). Sperm kinetics and membranes (plasma, acrosome and mitochondrial) were examined using the CASA system and triple fluorescence staining (PI/ PNA-FITC/Mitotracker). The progressive motility was greater (p < .05) with LC7.5 treatment than the control sperm at 96 hr. The curvilinear velocity (p < .01) and the percentage of sperm with intact membranes (plasma/acrosome/mitochondria) (p < .01) were greater with all LC treatments than the control group at all times. Straight line velocity was greater (p < .01) with LC5 and LC7.5 treatments than the control group after 48 hr. The LC5 group also returned lower ALH values (p < .05) than these seen for the control groups after 48 hr. The fertilizing capacity of LC5 samples stored at 15°C for 2 hr (LC5-15°C-2h) and at 5°C for 24 hr (LC5-5°C-24h) was tested in three ewe groups via cervical fixed-time artificial insemination. In two groups, the fertilizing capacity of the LC5-5°C-24h was reduced (p < .001). In the remaining group, however, no significant difference was seen between the LC5-15°C-2h and LC5-5°C-24h sperm in this respect (pregnancy rates 52.4% versus 42.8%; p > .05). Overall, the present results suggest that supplementing skimmed milk–egg yolk-based extenders with LC has a positive effect on chilled sperm variables and fertilizing capacity. 相似文献
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Escandón Byron M. Espinoza Juan S. Perea Fernando P. Quito Fernando Ochoa Rafael López Gonzalo E. Galarza Diego A. Garzón Juan Pablo 《Tropical animal health and production》2020,52(5):2523-2528
Tropical Animal Health and Production - New postpartum strategies have been developed in dairy cows to ameliorate uterine health and reproductive performance, especially the first service... 相似文献
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Total and mineralizable soil carbon as affected by tillage in the Argentinean Pampas 总被引：1，自引：0，他引：1
Alejandro Costantini Helvcio De-Polli Carlos Galarza Roberto Pereyra Rossiello Romina Romaniuk 《Soil & Tillage Research》2006,88(1-2):274-278
This study was carried out in experimental plots established at Marcos Juárez, in the Pampean Region (center of Argentina) on Typic Argiudolls, with high silt content. The aim of this work was to study the effects of two tillage systems (reduced tillage and no-tillage) on the amount of total organic C, potentially mineralizable C, C released by respiration, and C stock in the topsoil of a corn (Zea mays L.)–wheat (Triticum aestivum L.)/soybean (Glycine max L. Merr.)–soybean rotation. No-tillage showed C stock greater than reduced tillage only at 0–5 cm depth, but not at 0–20 cm, even though in situ respiration was lower. As a consequence, no tillage did not show a differential capacity for C sequestration in comparison with reduced tillage. 相似文献