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1.
Couacy-Hymann E Bodjo SC Danho T Koffi MY Libeau G Diallo A 《Preventive veterinary medicine》2007,78(1):85-88
We observed 15 goats for 9 days after subcutaneous infection with 10(3) TCID(50) with isolates of peste-des-petits ruminants virus from Africa and India and five concurrent, uninfected control goats. Typical clinical signs of the infection were present in all 15 infected goats by day 8 and in most by day 6 and some signs were present by day 4. However, 6 out of 15 goats already have detectable virus shedding by day 3 and four more were shedding by day 4 and every goat had virus shedding for at least 1 day before the recognition of clinical signs. This experiment indicates that incubatory carriers therefore might play a role in the transmission of PPRV among small ruminants. 相似文献
2.
Choi KS Nah JJ Choi CU Ko YJ Sohn HJ Libeau G Kang SY Joo YS 《Veterinary microbiology》2003,96(1):1-16
An experimental competitive enzyme-linked immunosorbent assay (morbillivirus cELISA) using a recombinant N antigen (rRPV N) expressed in a baculovirus and a ruminant morbillivirus (RPV and PPRV)-specific monoclonal antibody (P-13A9) was developed for simultaneous detection of rinderpest virus (RPV) and peste des petits ruminants virus (PPRV) antibodies and its diagnostic performance was evaluated. A set of known reference antisera against RPV and PPRV belonging to different lineages, experimental sera from cattle vaccinated for a RPV of Asian lineage, and field sera from cattle and sheep/goat populations known to be positive (West Africa) and negative (Korea) for RPV and PPRV were used for the evaluation. Morbillivirus cELISA results on the panel of experimental RPV and PPRV antisera showed high correlation (r=0.97) between the whole virus and the rRPV N antigens, suggesting that the rRPV N contains a ruminant morbillivirus-specific antigenic determinant recognized by the P-13A9 and it may be suitable as an ELISA antigen in place of the whole virus. Morbillivirus cELISA detected anti-RPV and anti-PPRV antibodies in all reference RPV and PPRV antisera containing VN titers >/=1:8, suggesting that the assay can simultaneously detect antibodies against RPV and PPRV. Anti-RPV antibody was detected by morbillivirus cELISA in vaccinated cattle as early as the VNT and continued to be detectable by both the cELISA and the VNT until termination of the study. When applied to field samples from Africa, morbillivirus cELISA showed good agreement with a RP cELISA kit (kappa value of 0.86) in bovine sera and with a peste des petits ruminant cELISA kit (kappa value of 0.81) in caprine/ovine sera. Usefulness of morbillivirus cELISA using the rRPV N protein was discussed. 相似文献
3.
Taylor WP Diallo A Gopalakrishna S Sreeramalu P Wilsmore AJ Nanda YP Libeau G Rajasekhar M Mukhopadhyay AK 《Preventive veterinary medicine》2002,52(3-4):305-312
Because previous authorities had suggested that small ruminants were playing a part in the dissemination of rinderpest, and a rinderpest-eradication campaign was about to begin, it was necessary to make precise virus identifications from a number of small-ruminant “rinderpest” outbreaks. When this was done using a database created from passive disease reports, we found that epidemics—reportedly due to rinderpest—were in fact due to peste des petits ruminants (PPRs). Although such cases had been common in India for a number of years, earlier clinical and laboratory reports no longer should be regarded as definitive. PPR outbreaks have been frequent in recent years. Further, we suggest that PPR is not a recent invader of India. 相似文献
4.
The distribution of seabirds west of Scotland was studied relative to water masses in August 1988. Surface salinity was used to indicate the ship's position relative to the water masses described in the literature. Comparisons were made between seabird densities in Inshore Water, the Northern Coastal Current, the Southern Coastal Current and Atlantic/Hebridean Water. Seabird density varied with water mass; some species distributions suggested strong habitat associations. Water masses represent marine habitats, with different physical regimes supporting different marine life; patterns in bird distribution are likely to reflect prey distribution and availability. Manx shearwaters Puffinus puffinus, razorbills Alca torda, guillemots Uria aalge, and kittiwakes Rissa tridactyla were found mostly in Inshore Water, with lower densities in the Northern and Southern Coastal Current water. Only trace numbers of these species occurred in Atlantic/Hebridean Water. Storm petrels Hydrobates pelagicus, fulmars Fulmarus glacialis and puffins Fratercula arctica were found in very low densities in Inshore Water, and were dispersed in higher densities throughout the more oceanic areas. These data suggest that in late summer the Inshore Water of southwestern Scotland has the highest densities of seabirds potentially vulnerable in the event of an oil incident. 相似文献
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6.
Large-scale deployment of a rice 6 K SNP array for genetics and breeding applications 总被引：1，自引：0，他引：1
Michael J. Thomson Namrata Singh Maria S. Dwiyanti Diane R. Wang Mark H. Wright Francisco Agosto Perez Genevieve DeClerck Joong Hyoun Chin Geraldine A. Malitic-Layaoen Venice Margarette Juanillas Christine J. Dilla-Ermita Ramil Mauleon Tobias Kretzschmar Susan R. McCouch 《Rice》2017,10(1):40
Background
Fixed arrays of single nucleotide polymorphism (SNP) markers have advantages over reduced representation sequencing in their ease of data analysis, consistently higher call rates, and rapid turnaround times. A 6 K SNP array represents a cost-benefit “sweet spot” for routine genetics and breeding applications in rice. Selection of informative SNPs across species and subpopulations during chip design is essential to obtain useful polymorphism rates for target germplasm groups. This paper summarizes results from large-scale deployment of an Illumina 6 K SNP array for rice.Results
Design of the Illumina Infinium 6 K SNP chip for rice, referred to as the Cornell_6K_Array_Infinium_Rice (C6AIR), includes 4429 SNPs from re-sequencing data and 1571 SNP markers from previous BeadXpress 384-SNP sets, selected based on polymorphism rate and allele frequency within and between target germplasm groups. Of the 6000 attempted bead types, 5274 passed Illumina’s production quality control. The C6AIR was widely deployed at the International Rice Research Institute (IRRI) for genetic diversity analysis, QTL mapping, and tracking introgressions and was intensively used at Cornell University for QTL analysis and developing libraries of interspecific chromosome segment substitution lines (CSSLs) between O. sativa and diverse accessions of O. rufipogon or O. meridionalis. Collectively, the array was used to genotype over 40,000 rice samples. A set of 4606 SNP markers was used to provide high quality data for O. sativa germplasm, while a slightly expanded set of 4940 SNPs was used for O. sativa X O. rufipogon populations. Biparental polymorphism rates were generally between 1900 and 2500 well-distributed SNP markers for indica x japonica or interspecific populations and between 1300 and 1500 markers for crosses within indica, while polymorphism rates were lower for pairwise crosses within U.S. tropical japonica germplasm. Recently, a second-generation array containing ~7000 SNP markers, referred to as the C7AIR, was designed by removing poor-performing SNPs from the C6AIR and adding markers selected to increase the utility of the array for elite tropical japonica material.Conclusions
The C6AIR has been successfully used to generate rapid and high-quality genotype data for diverse genetics and breeding applications in rice, and provides the basis for an optimized design in the C7AIR.7.
8.
Genevieve Patrick Andrea M. Tarnecki Nicole Rhody Ryan Schloesser Kevan Main Roy Yanong Ruth Francis‐Floyd 《Aquaculture Research》2019,50(12):3793-3801
Almaco jack (Seriola rivoliana Valenciennes) is an excellent candidate for aquaculture due to its fast growth rate and high market value. While S. rivoliana have adapted well to captivity, survival at early life stages can be improved to increase profitability during production. A wide range of variables cause larval mortalities but high bacterial loads in rearing tanks are often correlated with these losses. The aim of this study was to investigate the effect of egg disinfection on bacterial load and hatch rate of S. rivoliana. Disinfectants tested included formalin (F100 and F200; 100 and 200 mg/L, respectively, for 60 min), hydrogen peroxide (HPO; 300 mg/L for 10 min) and peracetic acid/hydrogen peroxide (PAA/HPO; 15.7 mg/L/39.6 mg/L for 1 min). Concentrations and contact times were determined based on current use in marine aquaculture and preliminary trials. Eggs treated with HPO and F100 had significantly higher hatch rates than the untreated control group. All treatments significantly decreased total Vibrio counts compared to untreated eggs; however, total bacterial counts were only decreased following treatments with PAA/HPO and F200. To prevent egg mortality due to bacterial overgrowth, consideration should be given to the use of surface disinfection using HPO or F100. Future studies should investigate the use of peracetic‐based products at lower doses. 相似文献
9.
Athanase K. Otchoumou Yao L. Alla Genevieve Corraze Sebastien L. Niamke Jacques K. Diopoh 《Journal Of Applied Aquaculture》2013,25(2):169-178
An 8-week feeding trial was carried out to determine the effect of increasing dietary palm oil levels on African catfish juvenile growth and nutrient utilization. Six isonitrogenous diets were formulated to contain 0.5% to 15% crude palm oil with dietary lipid levels ranging from 5% to 20%. Growth performance and protein utilization efficiency of African catfish responded in a positive manner to palm oil addition up to 9% (diet 14% lipid). No further improvement in growth was observed with higher levels of palm oil in the diets, and lower survival was observed with inclusion of 15% palm oil (diet 20% lipid). Whole-body lipid content increased when fish were fed with increasing levels of dietary lipid. Under our experimental conditions, the best growth rate and nutrient utilization were observed with the inclusion of 9% crude palm oil in the diets for African catfish. 相似文献
10.
Two types of in vitro assays (enzyme immunoassay and sero-neutralization test) were compared for their ability to detect antibodies to rinderpest virus in field sera from West African bovines. Purified virus grown on Vero cells (Ag/Vero) or bovine kidney cells (Ag/BK), were tested as antigen in enzyme-linked immunoassays (EIA). The results of the comparative evaluation of the two antigens by EIA, prove that Ag/Vero did enhance the sensitivity and the specificity of the test in comparison to Ag/BK and offers a 94% correspondence with seroneutralization. 相似文献