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Kirsty M Townsend Vo Ngan Giang Carol Stephens Paul T Scott Darren J Trott 《Journal of veterinary diagnostic investigation》2005,17(2):103-109
Sixty-nine intestinal spirochetes isolated from pigs and poultry in eastern Australia were selected to evaluate the effectiveness of a species-specific PCR-based restriction fragment length polymorphism (RFLP) analysis of the Brachyspira nox gene. For comparative purposes, all isolates were subjected to species-specific PCRs for the pathogenic species Brachyspira hyodysenteriae and Brachyspira pilosicoli, and selected isolates were examined further by sequence analysis of the nox and 16S ribosomal RNA genes. Modifications to the original nox-RFLP method included direct inoculation of bacterial cells into the amplification mixture and purification of the PCR product, which further optimized the nox-RFLP for use in a veterinary diagnostic laboratory, producing sufficient product for both species identification and future comparisons. Although some novel profiles that prevented definitive identification were observed, the nox-RFLP method successfully classified 45 of 51 (88%) porcine and 15 of 18 (83%) avian isolates into 5 of the 6 recognized species of Brachyspira. This protocol represents a significant improvement over conventional methods currently used in veterinary diagnostic laboratories for rapid specific identification of Brachyspira spp. isolated from both pigs and poultry. 相似文献
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宝交早生草莓在0℃下自然降氧贮藏10 d,好果率84%,商品率达98%。用10%CO_2处理可将草莓在0℃的贮藏时间延长到20 d,好果率和商品率均为94%,CO_2浓度超过20%会使草莓产生酒精味。SO_2慢性释放剂处理也有较好的防腐效果。 相似文献
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Geurden T Somers R Thanh NT Vien LV Nga VT Giang HH Dorny P Giao HK Vercruysse J 《Veterinary parasitology》2008,153(3-4):384-388
In northern Vietnam, dairy cattle are mainly managed in small-scale farms, where animals are kept confined and feeding occurs by cut and carry methods. In the present study the occurrence of parasitic infections was examined in five provinces around Hanoi. A total of 201 farms were visited, and 334 stool and 239 blood samples were collected from calves younger than 3 months, animals between 3 and 24 months and adult cows. Furthermore, 254 milk samples were collected from lactating animals. Coproscopical examination indicated a high prevalence of nematode eggs (Cooperia spp., Haemonchus and Oesophagostomum spp.) in animals (n=176) between 3 and 24 months (66%) and in adult cows (n=90; 54%). In these age groups the prevalence of Fasciola was 28% and 39%, respectively, and for Paramphistomum the prevalence was 78% and 82%, respectively. Fifty percent of the calves younger than 3 months (n=68) were positive for Giardia, and none for Cryptosporidium. Most Giardia isolates were identified as the non-zoonotic G. duodenalis assemblage E on the beta-giardin gene. The blood samples were examined with commercially available Svanovir((R))Elisa's for the presence of Anaplasma marginale and Babesia bigemina specific antibodies, and a prevalence of 28% and 54% was found, respectively. In the milk samples Neospora caninum specific antibodies (Svanovir((R))Elisa) were detected in 30% of the lactating animals. The present study demonstrates that parasitic infections occur frequently in dairy cattle around Hanoi although animals are mainly kept confined, and indicates that further research on the economic impact of these infections is needed. 相似文献
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Truong‐Giang Huynh Shao‐Yang Hu Chiu‐Shia Chiu Quoc‐Phu Truong Chun‐Hung Liu 《Aquaculture Research》2019,50(3):807-817
The aims of this study were to unravel the intestinal microbiota of Litopenaeus vannamei after being fed a diet without (control) or with the synbiotic (SYN) for 60 days using next‐generation sequencing technology to see if changes in the intestinal microbiota were involved in the improved growth performance and health status of the shrimp. Next‐generation sequencing data showed that six phyla, 11 classes, 19 orders, 30 families, 58 genera and 73 species with taxonomic names assigned were detected. The majority of the operational taxonomic units (OTUs) was shared between the SYN and control shrimp and comprised 37 OTUs. However, intestinal biodiversity analyses revealed that SYN‐fed shrimp had a higher species richness, evenness and Shannon–Weaver index than did shrimp fed the control diet, but without reaching statistical significance. Interestingly, shrimp fed the SYN diet exhibited improved colonization of Lactobacillus plantarum and reduced prevalences of Vibrio harveyi and Photobacterium damselae in the intestines. These findings indicate that the SYN was able to modulate the intestinal bacterial community of shrimp and could be used to control vibriosis in shrimp. 相似文献
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以毛竹Phyllostachys edulis集成材为研究对象,在不同条件下进行高频热压,通过对竹集成材板坯高频热压过程中芯层温度变化的统计分析,得到了竹集成材高频热压过程中板坯温度的变化规律。结果表明:在试验条件范围内,随着板坯含水率从6%增加到18%,涂胶量从200 g·m-2增加到300 g·m-2,板坯的温度明显升高。升温过程可以分为快速升温和慢速升温2个阶段。在快速升温阶段板坯内的温度随板坯初含水率、涂胶量及加热时间的提高而递增;在慢速升温阶段板坯初含水率及涂胶量对板坯内的温度的影响很小,板坯芯层升温速度随加热时间的增加而减少。通过试验数据分析,得出较优的高频热压胶合工艺条件为:涂胶量300 g·m-2,竹条含水率12%,高频热压时间7 min。 相似文献
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Despite being a leading shrimp exporter in the world market, shrimp farming in Vietnam is carried out by many small‐scale farmers who face constraints in complying with increasingly stringent food safety and sustainability standards. The best way for such small farmers to circumvent this paradox is to make effective integrations both horizontally and vertically. Using value chain and institutional economics approaches, this study determines the factors that influence the probability of horizontal and vertical integrations between the production and processing stages in the shrimp industry in Vietnam. Analysis results confirm that integrated shrimp aquaculture is much more effective than the traditionally small‐scale‐ and individual‐production mode. In addition, logit estimation results identify the key factors affecting integration, including effectiveness of the legal framework, production scale, infrastructure development, and farmers' skill and awareness. Several policy recommendations proposed to encourage the development of integration in shrimp farming in Vietnam could also be useful for other similar developing countries. 相似文献
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为研究水杉苗对氮素营养的需求 ,按 0 0 ,1 0 ,2 0 ,3 0 ,4 0和 5 0g氮 /盆 6个水平进行试验研究。结果表明 :水杉苗高、地径、分枝数、分枝总长等随着施氮量的增加而提高 ,但当施氮量超过 3 0 g/1 5kg土后 ,这些项目又随施氮量的继续增加而下降。水杉苗叶绿素的含量对氮素的反应亦与此类似 ;根、茎、叶的含氮量随施氮量的增加而增加。 相似文献