Safety, efficacy, and immunogenicity of a modified-live equine influenza virus vaccine in ponies after induction of exercise-induced immunosuppression     

Lunn DP  Hussey S  Sebing R  Rushlow KE  Radecki SV  Whitaker-Dowling P  Youngner JS  Chambers TM  Holland RE  Horohov DW 《Journal of the American Veterinary Medical Association》2001,218(6):900-906

OBJECTIVE: To determine safety, efficacy, and immunogenicity of an intranasal cold-adapted modified-live equine influenza virus vaccine administered to ponies following induction of exercise-induced immunosuppression. DESIGN: Prospective study. ANIMALS: Fifteen 9- to 15-month old ponies that had not had influenza. PROCEDURE: Five ponies were vaccinated after 5 days of strenuous exercise on a high-speed treadmill, 5 were vaccinated without undergoing exercise, and 5 were not vaccinated or exercised and served as controls. Three months later, all ponies were challenged by nebulization of homologous equine influenza virus. Clinical and hematologic responses and viral shedding were monitored, and serum and nasal secretions were collected for determination of influenza-virus-specific antibody isotype responses. RESULTS: Exercise caused immunosuppression, as indicated by depression of lymphocyte proliferation in response to pokeweed mitogen. Vaccination did not result in adverse clinical effects, and none of the vaccinated ponies developed clinical signs of infection following challenge exposure. In contrast, challenge exposure caused marked clinical signs of respiratory tract disease in 4 control ponies. Vaccinated and control ponies shed virus after challenge exposure. Antibody responses to vaccination were restricted to serum IgGa and IgGb responses in both vaccination groups. After challenge exposure, ponies in all groups generated serum IgGa and IgGb and nasal IgA responses. Patterns of serum hemagglutination inhibition titers were similar to patterns of IgGa and IgGb responses. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that administration of this MLV vaccine to ponies with exercise-induced immunosuppression was safe and that administration of a single dose to ponies provided clinical protection 3 months later.  相似文献   

Regional antibody and cellular immune responses to equine influenza virus infection,and particle mediated DNA vaccination   总被引：4，自引：0，他引：4  

Soboll G  Horohov DW  Aldridge BM  Olsen CW  McGregor MW  Drape RJ  Macklin MD  Swain WF  Lunn DP 《Veterinary immunology and immunopathology》2003,94(1-2):47-62

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Detection and quantification of equine herpesvirus-1 viremia and nasal shedding by real-time polymerase chain reaction.     

Stephen B Hussey  Rodney Clark  Katharine F Lunn  Cormac Breathnach  Gisela Soboll  J Millar Whalley  D Paul Lunn 《Journal of veterinary diagnostic investigation》2006,18(4):335-342

Equine herpesvirus-1 (EHV-1) infection is common in young horses throughout the world, resulting in respiratory disease, epidemic abortion, sporadic myelitis, or latent infections. To improve on conventional diagnostic tests for EHV-1, a real-time polymerase chain reaction (PCR) technique was developed, using primers and probes specific for the EHV-1 gB gene. Amplification efficiencies of 100% +/- 5% were obtained for DNA isolated from a plasmid, infected peripheral blood mononuclear cells (PBMCs), and nasal secretions from infected ponies. The dynamic range of the assay was 8 log10 dilutions, and the lower limit of detection was 6 DNA copies. Fifteen ponies, seronegative for EHV-1, were experimentally infected with EHV-1, and nasal samples were used to quantify shedding of virus by both virus isolation and real-time PCR analysis. Virus isolation identified nasal shedding of EHV-1 in 12/15 ponies on a total of 25 days; real-time PCR detected viral shedding in 15/15 ponies on 75 days. Viremia was quantified using PBMC DNA, subsequent to challenge infection in 3 additional ponies. Viremia was identified in 1/3 ponies on a single day by virus isolation; real-time PCR detected viremia in 3/3 ponies on 17 days. When real-time PCR was used to analyze PBMC DNA from 11 latently infected ponies (documented by nested PCR), EHV-1 was not detected. We conclude that real-time PCR is a sensitive and quantitative test for EHV-1 nasal shedding and viremia and provides a valuable tool for EHV-1 surveillance, diagnosis of clinical disease, and investigation of vaccine efficacy.  相似文献   

Einfluss assoziativer rhizosphärenbakterien auf die äHrstoffaufnahme und den Ertrag von Mais     

Gisela Höflich  Erik Tappe  Günter Kühn  Wolfgang Wiehe 《Archives of Agronomy and Soil Science》2013,59(4):323-333

In mehrjährigen Feldversuchen (1990–1995) in Nordostdeutschland wurde der Einfluß selektierter assoziativer Rhizosphärenbakterien auf den Ertrag und die Nährstoffaufnahme bei Mais untersucht.

Der Trockenmasse‐ und Kolbenertrag zur Siloreife bzw. der Samenertrag wurde durch die Bakterienstämme Pseudomonas fluorescens (PsIA12), Agrobacterium rhizogenes (A1A4), Rhizobium trifolii (R39) und z.T. durch Stenotrophomonas maltophilia (PsIB2) in den Jahren 1990 bis 1995 auf lehmigen Sandböden wiederholt signifikant, auf sandigem Lehm zum Teil signifikant erhöht. Maissorten können unterschiedlich auf die Bakterieninokulation reagieren. Die Bakterien stimulierten insbesondere bei Jungpflanzen die Wurzelentwicklung und die Nährstoffaufnahme (N, P, K) aus dem Boden. Sie bildeten Phytohormone (Auxine und z.T. Cytokinine) und überlebten, auch unter Feldbedingungen, im Rhizosphärenraum von Mais während der gesamten Vegetation.  相似文献   

Sectional notes     

H. G. Zuckerman  C. H. Metzger  B. A. Brown  W. B. Ward  Frank W. Hussey  R. W. Donaldson  E. E. Isaac  Marx Koehnke  William H. Martin  K. H. Fernow  E. V. Hardenburg  Robert Schmidt  E. M. Gillig  E. B. Tussing  E. R. Jackman  T. E. Odland  S. P. Swenson  John Tucker 《American Journal of Potato Research》1936,13(8):223-232

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Balaustium murorum (Hermann, 1804) (Acarina,Erythraeidae) in Häusern. Eine wenig beachtete sommerliche Milbenplage     

Gisela Rack 《Journal of pest science》1973,46(9):129-132

Zusammenfassung Fünf in der BRD erstmalig registrierte Fälle von mehr oder weniger starkem Auftreten vonBalaustium murorum (Hermann, 1804) in oder an Häusern, von denen Material der Verfasserin zur Untersuchung vorlag, werden aufgeführt. Ferner werden Beobachtungen von C. F.van de Bund in den Niederlanden und I. M.Newell in Nordamerika erwähnt. Bei letzteren ist wichtig, daß Vertreter der GattungBalaustium, von denen einige offensichtlich Pflanzenfresser sind, auch den Menschen angreifen und Bakterien übertragen können.

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Summary Five cases of more or less heavy appearance ofBalaustium murorum (Hermann, 1804) in or at houses are represented, at first observed in West Germany by the writer. Observations from the Netherlands and North America are mentioned. The latter ones are important because species ofBalaustium may feed on plants but also may attack man and transmit bacteria.

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Occurrence of naturally acetylated lignin units   总被引：2，自引：0，他引：2  

Del Río JC  Marques G  Rencoret J  Martínez AT  Gutiérrez A 《Journal of agricultural and food chemistry》2007,55(14):5461-5468

This work examines the occurrence of native acetylated lignin in a large set of vascular plants, including both angiosperms and gymnosperms, by a modification of the so-called Derivatization Followed by Reductive Cleavage (DFRC) method. Acetylated lignin units were found in the milled wood lignins of all angiosperms selected for this study, including mono- and eudicotyledons, but were absent in the gymnosperms analyzed. In some plants (e.g., abaca, sisal, kenaf, or hornbeam), lignin acetylation occurred at a very high extent, exceeding 45% of the uncondensed (alkyl-aryl ether linked) syringyl lignin units. Acetylation was observed exclusively at the gamma-carbon of the lignin side chain and predominantly on syringyl units, although a predominance of acetylated guaiacyl over syringyl units was observed in some plants. In all cases, acetylation appears to occur at the monomer stage, and sinapyl and coniferyl acetates seem to behave as real lignin monomers participating in lignification.  相似文献   

Proteomic Characterization of Equine Cerebrospinal Fluid     

Carolyn J. Broccardo  Gisela Soboll Hussey  Lutz Goehring  Paul Lunn  Jessica E. Prenni 《Journal of Equine Veterinary Science》2014

Cerebrospinal fluid (CSF) is a biofluid that is reflective of overall health. Although proteomic profiling of human CSF has been performed in the context of a variety of disease states, this report represents the first comprehensive proteomic analysis of equine CSF. A total of 320 proteins were confidently identified across six healthy horses, and these proteins were further characterized by gene ontology terms mapped in UniProt, and normalized spectral abundance factors were calculated as a measure of relative abundance. Theses results provide an optimized protocol for analysis of equine CSF and lay the groundwork for future studies involving the study of equine CSF in the context of pathogenic disease states.  相似文献   

THE MINOR PLANET DQ     

Hussey WJ 《Science (New York, N.Y.)》1898,8(202):673-674

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Identification of tomato cultivars (Lycopersicon esculentum) by polyacrylamide isoelectric focusing     

Gisela Henn  A. W. H. Neitz  A. I. Louw 《Euphytica》1992,62(2):77-82

Summary Isozyme analyses have been used for the definitive identification of many plant cultivars, but not for cultivated tomatoes. Six isozyme systems, namely alcohol dehydrogenase, acid phosphatase, phosphoglucomutase, esterase, phosphoglucoisomerase and 6-phosphogluconate dehydrogenase of tomato seed extracts were resolved by isoelectric focusing on polyacrylamide gels with a narrow pH gradient. Nine alcohol dehydrogenase phenotypes were distinguished which, with three acid phosphatase phenotypes, identified twelve of the seventeen cultivars. Fewer differences were found for the other isozymes. Since this method could differentiate between breeding parents and their progeny it is concluded that further investigations are warranted.Abbreviations APS acid phosphatase - ADH alcohol dehydrogenase - EST esterase - IEF isoelectric focusing - PGI phosphoglucoisomerase - PGM phosphoglucomutase - 6-PGDH 6-phosphogluconate dehydrogenase - RFLP restriction fragment length polymorphism - VOPRI Vegetable and Ornamental Plant Research Institute  相似文献