排序方式： 共有57条查询结果，搜索用时 15 毫秒
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M. Lodovica Gullino Riccardo Savigliano Giuliana Gasparrini Corrado Clini 《Phytoparasitica》2007,35(4):321-329
Methyl bromide (MB) was phased out on January 1, 2005, and a Critical Use Exemption (CUE) process has been established in
order to define the rules for allocating the needed amounts of the fumigant. This paper deals with the effects of the CUE
process on Italian horticulture. The actual usage of the amounts of MB allocated to Italy for the year 2005 in the different
areas and on the economically most important crops was monitored and critically evaluated. The usage pattern monitored shows
that already in the first year after MB phaseout, Italy was able to replace it completely on crops such as zucchini, salad
greens, basil and watermelon. However, on crops such as tomato, pepper, melon, eggplant, strawberry, and cut flowers, further
work is required in order fully to replace MB. The emerging problems and research needs are discussed briefly. 相似文献
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The effect of foliar application of cytokinin (benzyl-adenine, 150 mg L−1) and gibberellin (GA4 + GA7, 150 mg L−1) on growth and flower development of 5-year-old plants of two jojoba clones was studied. The plant growth regulators were applied on October 5, 1999 (spring) and the plants were evaluated 120, 240 and 360 days after application. Shoot length, total number of nodes and number of nodes with branching were statistically different between clones but not between the growth regulator treatments.The total number of flowers on both clones was significantly increased by treatment with benzyl-adenine (BA) and significantly reduced by treatment with gibberellin. The seed yields, evaluated 180 days after application, were not statistically different from the control due to an increase in flower abortion. One clone treated with gibberellin showed a significant decrease in number and weight of seeds, the other did not.Histology of axillary buds revealed that BA application on one clone (4.11.32) enlarged the flower meristem, differentiating multiple flower production. 相似文献
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Emanuele Ponis René Robert Giuliana Parisi Mario Tredici 《Aquaculture International》2003,11(1-2):69-79
The Prymnesiophycea Pavlova lutheriwas produced using a traditional technique (batch mode in carboys) and by an innovative technique (semi-continuous mode in alveolarphotobioreactor). Two experimental feedingtrials were carried out with Pacific oyster(Crassostrea gigas) larvae with freshand preserved (4 °C and 1 °C)P. lutheri concentrates used inmonospecific and bispecific diets, incombination with fresh Chaetoceroscalcitrans forma pumilum. Nodifferences in terms of gross composition andlarval performance were noticed between thefresh algae biomass produced by the twotechniques. The low temperatures allowedpreservation of the nutritionalcharacteristics of the P. lutheri freshculture for the time period considered (27days). The P. lutheri concentratespreserved at 4 °C or 1 °C didnot differ substantially in terms ofnutritional value. Larvae fed a monospecificdiet of P. lutheri, both fresh andpreserved, exhibited a modest growth rate (<2.1 µm day–1), in some cases notsignificantly different from that observed inthe unfed larvae (the control), and a lowsurvival rate. In contrast, when Chaetoceros calcitrans forma pumilumwas used as a monospecific diet, good growthperformance (>4.5 µm day–1) and ahigh survival rate (>86%) were observed inlarvae. A substitution of 50% (trial 1) or80% (trial 2) of fresh C. calcitransforma pumilum with the preserved P. lutheri concentrates did not adverselyaffect growth rate or survival of C.gigas larvae. 相似文献
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Brovelli EA Rua D Roh-Schmidt H Chandra A Lamont E Noratto GD 《Journal of agricultural and food chemistry》2005,53(21):8156-8161
A phenological study was conducted to determine the impact of harvest maturity on the immune-modulating properties of Echinacea purpurea. The aerial parts of this plant were collected during seven stages of development and were assayed for a common botanical marker for this species, cichoric acid. Plants of selected development stages were also assayed for total polysaccharides and compared for their immune-modulating effects on the THP-1 monocyte/macrophage cell line by means of a gene expression study. Although the concentration of cichoric acid did not change significantly during the course of the study, stage 1 (advanced vegetative) had the highest concentration of total polysaccharides and exhibited the most potent induction activity on immune-modulating cytokines such as interferon-gamma and tumor necrosis factor-alpha. These findings suggest that the use of gene expression may be an effective tool not only to standardize botanical extracts but also to optimize harvest time. 相似文献
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Carlos?Wildt Oriol?Gibert José?Luis?CortinaEmail author Giuliana?Coscera 《Journal of Soils and Sediments》2004,4(3):184-191
Goal, Scope and Background The remediation of heavy-metalcontaminated soils and sediments is of significant value to industrial areas around the world.
The spread of such pollutants can result in a potential risk of entering the groundwater system and being transported to potential
receptors. Leaching techniques can be an effective treatment option for the metal removal from soils and sediments. This approach
consists of washing or leaching the contaminated soil with an appropriate reagent and the subsequent treatment of the leaching
in an above-ground installation (on-site treatment) where metals can be removed and concentrated into a smaller volume. Among
the heavy metals, chromium is a commonly identified soil contaminant, particularly in sites with intensive economic activities
including agriculture, industrial, mining and mineral,processing.
Objective The objective of this work was the evaluation and development of a leaching process for the remediation of soils and sediments
polluted with chromium at laboratory scale. Chromium soil pollution was generated after the breakdown of a channel containing
chromium wastes from a tannery plant. The pollution extension has been estimated to be on the order of thousands of tonnes
of soil to be treated, with chromium contents ranging from 500 to 17,000 mg kg-1 soil.
Methods The whole process investigated in this study integrates three stages; a) chromium leaching from a sediment using a diluted
sulphuric acid solution, b) treatment of the leaching effluents with a magnesium oxide/limestone mixture for the precipitation
of chromium hydroxide after acidity neutralisation, and c) polishing step to remove the eventual remaining chromium by adsorption
onto natural zeolite. The amount of contaminated sediment treated ranged from 0.5 to 2 kg with chromium contents of between
2000 and 17,000 mg kg-1.
Results and Discussion The paper describes results on the performance of the process and the optimisation of steps including influence of acid sulphuric
concentration, chromium removal efficiency as well as alkaline reactive mixture proportions. Effluents from the leaching cells
showed a significant decay on the chromium concentration with the increase of leaching runs and a high content of acidity
(pH values close to 0.5). The treatment of these effluents in a second cell containing magnesium oxide/ limestone mixtures
resulted in a high efficiency in neutralisation of acidity (pH values around 7) and chromium removal (concentrations below
5 mg 1-1). The passage through a third compartment containing zeolite as an adsorbent decreased the chromium concentration below 0.5
mg 1-1,
Conclusions From the results obtained on the chromium leaching and immobilisation with magnesium oxide/limestone mixture at a laboratory
scale, it could be pointed out that: (a) diluted sulphuric acid solutions (3%) demonstrated a high efficiency on chromium
removal from sandy polluted soils on the kilogram scale, (b) mixtures of magnesium oxide/limestone demonstrated a high capacity
to neutralise the residual high acidity present on the effluents and to remove chromium by precipitation and (c) between the
limestone and caustic magnesia mixtures, those containing more than 60% of caustic magnesia provide the higher efficiency.
Recommendation and Outlook Future work would be directed to the evaluation of the integrated process of leaching and chromium precipitation on column
at a scale of 100 to 1000 kg. 相似文献
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Kochendoerfer GG Chen SY Mao F Cressman S Traviglia S Shao H Hunter CL Low DW Cagle EN Carnevali M Gueriguian V Keogh PJ Porter H Stratton SM Wiedeke MC Wilken J Tang J Levy JJ Miranda LP Crnogorac MM Kalbag S Botti P Schindler-Horvat J Savatski L Adamson JW Kung A Kent SB Bradburne JA 《Science (New York, N.Y.)》2003,299(5608):884-887
We report the design and total chemical synthesis of "synthetic erythropoiesis protein" (SEP), a 51-kilodalton protein-polymer construct consisting of a 166-amino-acid polypeptide chain and two covalently attached, branched, and monodisperse polymer moieties that are negatively charged. The ability to control the chemistry allowed us to synthesize a macromolecule of precisely defined covalent structure. SEP was homogeneous as shown by high-resolution analytical techniques, with a mass of 50,825 +/-10 daltons by electrospray mass spectrometry, and with a pI of 5.0. In cell and animal assays for erythropoiesis, SEP displayed potent biological activity and had significantly prolonged duration of action in vivo. These chemical methods are a powerful tool in the rational design of protein constructs with potential therapeutic applications. 相似文献
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Giovanna Romano Emiliano Manzo Gian Luigi Russo Giuliana d’Ippolito Adele Cutignano Maria Russo Angelo Fontana 《Marine drugs》2013,11(11):4527-4543
Oxylipins are a large and diverse family of fatty acid derivatives exhibiting different levels of oxidation of the carbon chain. They are involved in many biological functions in mammals, plants and diatoms. In this last group of organisms, they are suggested to play a role in the reproductive failure of copepod predators, showing clear pro-apoptotic effects on newborn nauplii. In this work, these compounds were tested for the ability to induce mitotic arrest in sea urchin embryos. We show for the first time that oxylipins have an increased efficacy in their corresponding methylated form. Natural oxylipins were also used as an inspiration for the rational design and synthesis of stable chemical analogs with apoptotic activity against tumor cell lines. This approach led to the synthesis of the linear C15-ketol (22) that was shown to induce apoptosis in human leukemia U-937 cells. These results are proof of the concept of the use of eco-physiological considerations as a platform to guide the search for novel drug candidates. 相似文献
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Genoveffa Nuzzo Carmela Gallo Giuliana d’Ippolito Adele Cutignano Angela Sardo Angelo Fontana 《Marine drugs》2013,11(10):3742-3753
Accurate characterization of biomass constituents is a crucial aspect of research in the biotechnological application of natural products. Here we report an efficient, fast and reproducible method for the identification and quantitation of fatty acids and complex lipids (triacylglycerols, glycolipids, phospholipids) in microalgae under investigation for the development of functional health products (probiotics, food ingredients, drugs, etc.) or third generation biofuels. The procedure consists of extraction of the biological matrix by modified Folch method and direct analysis of the resulting material by proton nuclear magnetic resonance (1H NMR). The protocol uses a reference electronic signal as external standard (ERETIC method) and allows assessment of total lipid content, saturation degree and class distribution in both high throughput screening of algal collection and metabolic analysis during genetic or culturing studies. As proof of concept, the methodology was applied to the analysis of three microalgal species (Thalassiosira weissflogii, Cyclotella cryptica and Nannochloropsis salina) which drastically differ for the qualitative and quantitative composition of their fatty acid-based lipids. 相似文献