Effect of heterogeneous digesta chemical composition on the accuracy of measurements of fiber flow in dairy cows.     

S Ahvenj?rvi  B Skiba  P Huhtanen 《Journal of animal science》2001,79(6):1611-1620

The objective of the current study was to evaluate the effect of unrepresentative sampling of digesta particulate matter entering the omasal canal on the accuracy of fiber flow measurements. The experimental design comprised one period, one diet, and three cows as experimental units. Within each cow, the physical and chemical composition of digesta particulate matter was assessed at seven sites within the digestive tract. Three Finnish Ayshire dairy cows, equipped with ruminal and simple-T duodenal cannulas, in extended lactation were offered grass silage twice daily on an ad libitum basis. Digesta samples were collected from the rumen (dorsal and ventral sac), reticulum, omasal canal, omasum, duodenum, and rectum to determine particle size distribution in digesta, chemical composition of various particle size fractions, and distribution of two flow markers (Cr-labeled straw and indigestible NDF [INDF]) among particle size fractions. Digesta samples were wet-sieved using sieves of 2.50, 1.25, 0.630, 0.315, 0.160, and 0.080 mm. Particulate matter was analyzed for OM, NDF, and Cr concentrations, and INDF concentration was determined based on 12-d ruminal incubation. The particle size of digesta entering the omasal canal was larger compared with the omasum or the duodenum, suggesting that omasal canal samples were not representative of particle size distribution truly escaping the rumen. The concentration of potentially digestible NDF (PDNDF) decreased with decreasing particle size. The PDNDF concentration of particulate matter retained on all sieves was greatest in the rumen and gradually decreased along the digestive tract. From the reticulorumen to the omasum, the decrease was associated with decreased particle size, reflecting selective passage of particulate matter. In contrast, from the omasum to the duodenum and rectum, the PDNDF concentration decreased within each particle size fraction without effect on particle size, indicating a nonselective passage of particulate matter between these sites. Variation between particle size fractions was slightly greater for Cr concentration than for INDF concentration, indicating that unrepresentative sampling of particulate matter had a greater effect on Cr concentration compared with that of INDF. Owing to unrepresentative sampling, NDF entering the omasal canal was overestimated by 5% using INDF and underestimated by 7% using Cr as a particle phase marker. Of total NDF digestibility, proportionally 0.90, 0.07, and 0.03 occurred in the reticulorumen, omasum, and intestines, respectively. The current results indicate that, despite unrepresentative sampling of digesta particulate matter entering the omasal canal, the errors in determined NDF flow were small. The omasum may have a greater role in postruminal NDF digestion than the intestines.  相似文献   

Predicting non‐native fish dispersal under conditions of climate change: case study in England of dispersal and establishment of pumpkinseed Lepomis gibbosus in a floodplain pond     

Emily Fobert  Grzegorz Zięba  Lorenzo Vilizzi  Michael J. Godard  Michael G. Fox  Saulius Stakėnas  Gordon H. Copp 《Ecology of Freshwater Fish》2013,22(1):106-116

Predictions of future climate change include shifts in patterns of precipitation, evapotranspiration and water run‐off, resulting in increased periods of drought as well as variability and intensity of rainfall events. In the United Kingdom, the non‐native North American sunfish, pumpkinseed Lepomis gibbosus (L.), is expected to benefit from these changes. We examine how hydrological variability induced by predicted changes in climate will affect the dispersal and spread of pumpkinseed in England by: (i) determining the relationship between discharge regime and pumpkinseed propagule pressure; (ii) examining a newly‐established pumpkinseed population following a flood event in 2007; and (iii) comparing the growth and life‐history traits of this new population with fish collected from the source population to demonstrate how the pumpkinseed's life‐history plasticity contributes to its success as a coloniser. Using Bayesian modelling, we determined that the number of pumpkinseed escapees is likely to increase with increasing discharge. The newly‐established pumpkinseed population showed fast juvenile growth, early age at maturity and small size at maturity. These traits differed significantly from the source population, specifically total length (TL) means at ages 1 and 2 were significantly greater in the new population, whereas TL at age 4 was significantly greater in the source population, and a significantly higher proportion of mature females were found at smaller size classes in the newly established pumpkinseed population. This study demonstrates the potential link between hydrological variability (current and future) and the dispersal of non‐native pumpkinseed, leading to the establishment of new populations.  相似文献   

Cohabitation of tree holes by ants and breeding birds in a temperate deciduous forest     

Sławomir Mitrus  Grzegorz Hebda  Tomasz Wesołowski 《Scandinavian Journal of Forest Research》2016,31(2):135-139

A previously unknown association of ants with birds breeding in tree holes is described. Ants Lasius brunneus Latreille (Hymenoptera: Formicidae) were found in c. 15% of nests of Parus major L. and Poecile palustris L. (Paridae) breeding in tree holes in the primeval deciduous forest located in the Bia?owie?a National Park (Poland). The ants preferably used holes located higher above the ground. As such holes are warmer than the unused holes or other nest sites, it is suggested that the ants cohabit holes utilized by the tits to gain thermal benefits.  相似文献   

Survival,growth and photochemical efficiency of silver fir seedlings produced with different technologies     

Robakowski  Piotr  Pietrzak  Tomasz  Kowalkowski  Wojciech  Małecki  Grzegorz 《New Forests》2021,52(6):1055-1077

New Forests - Forest tree seedling production technologies impact reforestation success determined with survival and quality of seedlings. Five Abies alba seedling production technologies were...  相似文献   

PPR‐B‐31: a new maintainer allele in the male‐fertility restorer gene of radish (Raphanus sativus) Ogura cytoplasm          下载免费PDF全文

Anna Hawliczek‐Strulak  Grzegorz Bartoszewski  Renata Słomnicka  Aleksandra Korzeniewska  Régine Delourme  Iwona Bartkowiak‐Broda  Katarzyna Niemirowicz‐Szczytt 《Plant Breeding》2015,134(5):557-563

The PPR‐B gene is responsible for male‐fertility restoration of the Ogura‐type male‐sterile radish plants, and it is located in the complex Rfo locus in the vicinity of similar PPR‐A gene and PPR‐C pseudogene. The aim of this study was to identify PPR‐B alleles and understand the structure of the Rfo locus in radish breeding lines. Five lines of radish with normal male‐fertile cytoplasm were tested. The entire PPR‐B gene was amplified, sequenced and allelic PPR‐B sequences were identified. The results indicated that the maintainer lines 7, 15 and 21 contained a non‐restoring form of PPR‐B protein. A unique PPR‐B was found in lines 24/15 and 31 that are restorer and maintainer lines, respectively. The substitutions might be responsible for the loss of a restoring function of the PPR‐B‐31 allele. Amplification of the PPR‐A/PPR‐B and PPR‐B/PPR‐C intergenic regions allowed to identify rearrangements within Rfo locus. Obtained results confirm the wide allelic variation within the Rfo locus, as well as high genetic complexity of the fertility restoration mechanism in radish.  相似文献   

Comment on "Impact ejecta layer from the mid-Devonian: possible connection to global mass extinctions"     

Racki G  Koeberl C 《Science (New York, N.Y.)》2004,303(5657):471; author reply 471

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Effect of improved fallow on crop productivity, soil fertility and climate-forcing gas emissions in semi-arid conditions     

Nicola M. Hall  Bocary Kaya  Jan Dick  Ute Skiba  Amadou Niang  Ramadjita Tabo 《Biology and Fertility of Soils》2006,42(3):224-230

The impacts of fallow on soil fertility, crop production and climate-forcing gas emissions were determined in two contrasting legumes, Gliricidia sepium and Acacia colei, in comparison with traditional unamended fallow and continuous cultivation systems. After 2 years, the amount of foliar material produced did not differ between the two improved fallow species; however, grain yield was significantly elevated by 55% in the first and second cropping season after G. sepium compared with traditional fallow. By contrast, relative to the unamended fallow, a drop in grain yield was observed in the first cropping season after A. colei, followed by no improvement in the second. G. sepium had higher foliar N, K and Mg, while A. colei had lower foliar N but higher lignin and polyphenols. In the third year after fallow improvement, a simulated rainfall experiment was performed on soils to compare efflux of N₂O and CO₂. Improved fallow effects on soil nutrient composition and microbial activity were demonstrated through elevated N₂O and CO₂ efflux from soils in G. sepium fallows compared with other treatments. N₂O emissions were around six times higher from this nitrogen-fixing soil treatment, evolving 69.9 ngN₂O–N g⁻¹soil h⁻¹ after a simulated rainfall event, compared with only 8.5 and 4.8 ngN₂O–N g⁻¹soil h⁻¹ from soil under traditional fallow and continuous cultivation, respectively. The findings indicate that selection of improved fallows for short-term fertility enhancement has implications for regional N₂O emissions for dry land regions.  相似文献   

Dynamics of arsenic-containing compounds sorption on sediments     

Reczynski  Witold  Posmyk  Grzegorz  Nowak  Katarzyna 《Journal of Soils and Sediments》2004,4(2):95-100

Background  River and lake sediments constitute complex and difficult analytical samples. On the other hand, sediments play a fundamental role in the distribution of toxic compounds in aquatic systems and in the evaluation of the current state and the course of changes taking place in the environment. Among elements present in the environment in trace concentrations, but having well-elaborated toxic properties, one of most dangerous is arsenic. The element occurs in the environment in several chemical forms, predominant are inorganic forms of As(V) and As(III), and methylated forms such as monomethylarsonic acid (MMAA) and dimethylarsinic acid (DMAA). Objectives  Reported herein are experiments, which were undertaken with the aim of examining the dynamics of arsenic sorption in sediments and its dependence on speciation of the element and the pH in the environment. Simultaneously, influence of organic matter content and chemical composition of the sediments on As sorption, were investigated. Methods  Sediment samples (upper 10 cm layer) were collected from three sites located in the vicinity of Cracow:

•	Rudawa river — 37 km long river, flowing mainly through the suburban area;
•	Vistula river — heavily contaminated, main Polish river;
•	Dobczyce reservoir — artificial reservoir on Raba river, total capacity of 125,000,000 m3, supplying about 60% of drinking water to the city of Cracow.

Using XRD and IR methods, mineral composition of sediments was analysed. Concentrations of iron, manganese, aluminium and arsenic as well as organic matter content in solid samples were analysed. Sorption experiment  100 g of dry sediment sample was placed in a 400 ml volume glass beaker. 300 ml of arsenic containing solution was poured into the beaker. Every 30 min (total experiment time span equalled 7 to 7.5 hours), 0.5 ml of the suspension’ (the beaker content was constantly mixed) was collected and centrifuged, and the concentration of arsenic was determined in the supernatant. In the series of experiments, solution contained about 0.05 μg/ml of arsenic in organic (DMAA) and inorganic forms, at different pH values — 3, 5, 7 and 9. Results and Discussion  Examined sediments of Vistula river and Dobczyce Lake were characterised by relatively high concentrations of arsenic, iron, manganese and aluminium.Rudawa river. At pH 3, the concentration of inorganic As in solution decreased from an initial value of 0.049 μg/ml to 0.012 μg/ml in 7.5 hours time. The same decreasing tendency was found at pH 5 (initial value 0.046 μg/ml, after 7.5 hours - 0.015 μg/ml). Less distinct sorption effects were noted at pH 7 and 9. Vistula river. At all pH values, a very significant decrease of inorganic As concentration up to 80% of the initial value, as well as a short time of achieving an equilibrium state (1 – 2.5 hours), was found. Dobczyce lake. Inorganic As was distinctly accumulated in sediments at pH 3 (decrease of As concentration in solution from 0.052 to 0.007 μg/ml after 7.5 hours of the experiment) and at pH 5. At pH 7, about 40% of As was accumulated in sediments (solution As concentration decrease from 0.050 to 0.030). Only 20% of As adsorbed on sediments at pH 9.Very weak accumulation of organic form of As (DMAA) was found in all examined sediments at pH 3 and 5. At higher pH values no sorption was registered. Conclusions  The following facts were established:

•	inorganic form of arsenic was effectively accumulated in all analysed sediments;
•	inorganic As sorption in sediments strongly depended on pH — for sediments of Rudawa river and Dobczyce lake, the highest sorption was found at pH 3, and a decrease of accumulated As was noted with an increase of pH (at pH 9, the lowest amount of As was immobilised in sediments); in the case of Vistula river sediments, a reverse tendency was found;
•	organic form of arsenic (DMAA) was accumulated only at pH 3 in all sediments and less significantly at pH 5 (Vistula river sediments); no sorption of that form of As was found in the other sediment samples;
•	state of equilibrium (no change in inorganic As concentration in liquid medium) was achieved in 1 hour for Vistula river sediments, after about 2 hours for Rudawa river and after about 4 hours for Dobczyce lake sediments.

Recommendations  The presented method of analysis of As sorption in sediments and results visualization (based on a newly defined coefficient) provides a useful tool for the characterization of sorptive properties of sediments and may be used for other compounds as well. Crucial, functional information — how much of a given element may be accumulated in sediments at a certain pH — is obtained without digestion of the sediment sample, in an experiment taking less than one day.  相似文献   

Mapping of the <Emphasis Type="Italic">ms8</Emphasis> male sterility gene in sweet pepper (<Emphasis Type="Italic">Capsicum annuum</Emphasis> L.) on the chromosome P4 using PCR-based markers useful for breeding programmes     

Grzegorz Bartoszewski  Cezary Waszczak  Piotr Gawroński  Iwona Stępień  Hanna Bolibok-Brągoszewska  Alain Palloix  Véronique Lefebvre  Aleksandra Korzeniewska  Katarzyna Niemirowicz-Szczytt 《Euphytica》2012,186(2):453-461

The nuclear male sterility gene ms8 is expected to facilitate the production of sweet pepper (Capsicum annuum L.) hybrids as it provides means for hybridization without the labor-intensive hand emasculation of female inbred lines. The development of molecular markers linked to ms8 locus will help the breeding practice for the selection of hybrid parental lines. In this study, F₂ population resulting from a cross between the sweet pepper male sterile line 320 and the male fertile variety Elf was used to identify DNA markers linked to the ms8 locus. With the use of RAPD–BSA technique, seven markers linked to the ms8 locus were found. Four of them were converted into SCAR markers. In addition, two COSII/CAPS markers linked to the ms8 locus were identified. Comparative mapping with reference pepper maps indicated that the ms8 locus is located on the lower arm of the pepper chromosome P4. Identified markers are useful for molecular breeding, however, at present markers tightly linked to ms8 locus are still lacking. Identification of molecular markers linked to the ms8 locus and determination of its chromosomal localization are useful for fine mapping and also provide the perspective for ms8 gene cloning.  相似文献   

The contribution of agricultural practices to nitrous oxide emissions in semi-arid Mali     

J. Dick    B. Kaya    M. Soutoura    U. Skiba    R. Smith    A. Niang  & R. Tabo 《Soil Use and Management》2008,24(3):292-301

The yield and flux of nitrous oxide (N₂O) emitted from continuous cereals (with and without urea), legumes/cereal in rotation and cereal/legume in rotation all with or without organic manure was monitored from January 2004 to February 2005. All treatments except continuous cereals had phosphate added. The cereal grown July–October in 2003 and 2004 was pearl millet ( Pennisetum glaucum) and the legume was a bean ( Phaseolus vulgaris ). The 10 m × 10 m plots were established in a semi-arid climate in Mali. The addition of organic manure and both inorganic fertilizers increased yield and N₂O emissions. Continuous cereals treated with both organic manure and urea emitted significantly less N₂O (882 g N/ha per year) than plots receiving no organic manure(1535 g N/ha per year). Growing N-fixing crops in rotation did not significantly increase N₂O emissions. This study supports the new practice of growing cereal and legumes in rotation as an environmentally sustainable system in semi-arid Mali.  相似文献