Editorial     

C. Guillermo Couto 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(2):71-71

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Evaluation of Olive Cultivars for Resistance to Verticillium dahliae     

F.J. López-Escudero  C. del Río  J.M. Caballero  M.A. Blanco-López 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(1):79-85

Resistance of 23 important olive cultivars to Verticillium dahliae has been evaluated in four experiments under controlled conditions. Nine-month-old nursery olive plants were inoculated with a cotton non-defoliating (ND) (V4) or a cotton defoliating (D) (V117) isolate of V. dahliae. Resistance was evaluated by assessing symptom severity using a 0–4 rating scale and estimating the area under disease progress curves. The percentage of plants killed and of those which recovered from the disease were used as additional parameters for including a particular cultivar into a defined category. Most of the evaluated cultivars were susceptible, although at different levels, to both isolates of V. dahliae. All cultivars were more susceptible to the D pathotype than to the ND one. A group of 11 cultivars, including several important Spanish cultivars, were susceptible or extremely susceptible to both pathotypes of V. dahliae. A second group showed differences of resistance depending on the pathotype used. They were susceptible or extremely susceptible to the D pathotype but resistant or moderately susceptible to the ND one. Finally, 'Frantoio', 'Oblonga' and 'Empeltre' were moderately susceptible to the D isolate of V. dahliae and resistant to the ND one. The resistance of 'Empeltre' was evident by the plant ability to recover from infection with either isolates. 'Empeltre' is considered to be a valuable cultivar for inclusion in breeding programmes for resistance to Verticillium wilt.  相似文献   

Stem cell factor supports migration in canine mesenchymal stem cells     

Nathaly Enciso  Luciana L. K. Ostronoff  Guillermo Mejías  Leticia G. León  María Luisa Fermín  Elena Merino  Cristina Fragio  Luis Avedillo  Concepción Tejero 《Veterinary research communications》2018,42(1):29-38

Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC). We also analyzed the role of Stem Cell Factor (SCF) on MSC proliferation and on ASCs metalloproteinases MMP-2, MMP-9 secretion. Healthy dogs were used as BMMSC donors, and ASC were collected from omentum during elective ovariohysterectomy surgery. Both cell types were cultured in IMDM medium with or without SCF, 10% Dog Serum (DS), and incubated at 38 °C with 5% CO2. Growth of BMMSCs and ASCs was exponential until 25–30 days. Flow citometry of MSCs revealed positive results for CD90 and negative for CD34, CD45 and MCH-II. Genes were evaluated by RT-PCR and metalloproteinases by zymografy. Our findings indicate morphological and immunological similarities as well as expression of genes from both origins on analyzed cells. Furthermore, SCF did not affect proliferation of MSCs, however it up-regulated MMP-2 and MMP-9 secretion in ASCs. These results suggest that metalloproteinases are possibly essential molecules pivoting migration.  相似文献   

Preliminary insights into the incorporation of rosemary extract (Rosmarinus officinalis L.) in fish feed: influence on performance and physiology of gilthead seabream (Sparus aurata)     

A. Hernández  B. García García  M. J. Caballero  M. D. Hernández 《Fish physiology and biochemistry》2015,41(4):1065-1074

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Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp. berkhoffii   总被引：1，自引：0，他引：1  

Diniz PP  Maggi RG  Schwartz DS  Cadenas MB  Bradley JM  Hegarty B  Breitschwerdt EB 《Veterinary research》2007,38(5):697-710

The purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the S?o Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer (ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples (n=8) were inoculated into a liquid pre-enrichment growth medium (BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog (ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii (pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs.  相似文献   

Genetic parameters for spawning and growth traits in the Pacific white shrimp (Penaeus (Litopenaeus) vannamei)          下载免费PDF全文

Alejandra Caballero‐Zamora  Eugenia Guadalupe Cienfuegos‐Rivas  Hugo H Montaldo  Gabriel Ricardo Campos‐Montes  Alfonso Martínez‐Ortega  Héctor Castillo‐Juárez 《Aquaculture Research》2015,46(4):833-839

The aim of this study was to estimate genetic parameters for spawning traits and growth traits in a breeding line of Pacific white shrimp, Penaeus (Litopenaeus) vannamei, selected for growth and survival. Traits studied were number of eggs (NE) and number of nauplii (NN) and female body weight at insemination (FWI) and body weight at 130 days of age (BW130). Genetic parameters were estimated using a multivariate animal model. Heritability for NE and NN were estimated as 0.13 ± 0.04 and 0.03 ± 0.04 respectively. The contribution to NN total variation due to ‘factors associated with male’ effect was estimated as 0.47 ± 0.07. In the cases of FWI and BW130, heritability was estimated as 0.44 ± 0.08 and 0.19 ± 0.03 respectively. Genetic correlation between FWI and NE was estimated as 0.49 ± 0.15, between FWI and NN as 0.54 ± 0.39 and between NE and NN as 0.27 ± 0.41, whereas the genetic correlations of FWI, NE and NN with BW130 were 0.30 ± 0.13, ?0.21 ± 0.19 and ?0.25 ± 0.38 respectively. Although it is important to perform more studies on this issue, our results found no evidence of a genetic antagonistic effect between female reproductive traits and body weight at harvesting (130 days of age) in P. vannamei.  相似文献   

Interannual differences in growth and hatch‐date distributions of early juvenile European anchovy in the Bay of Biscay: implications for recruitment          下载免费PDF全文

Naroa Aldanondo  Unai Cotano  Nerea Goikoetxea  Guillermo Boyra  Leire Ibaibarriaga  Xabier Irigoien 《Fisheries Oceanography》2016,25(2):147-163

In order to understand better the recruitment variability in European anchovy in the Bay of Biscay, it is important to investigate the processes that affect survival during the early life stages. Anchovy juvenile growth trajectories and hatch‐date distributions were inferred over a 3‐year period based on otolith microstructure analysis. Otolith growth trajectories showed a characteristic shape depending on their hatch‐date timing. Earlier‐born juveniles had notably broader maximum increments than later born conspecifics, resulting in higher growth rates. This observation suggests that early hatching would be beneficial for larval and juvenile growth, and, therefore, survival. The estimated juvenile hatch‐date distributions were relatively narrow compared with the extended anchovy spawning season (March–August) in the Bay of Biscay and indicated that only individuals originated mainly from the summer months (June–August) survived until autumn. Hatch‐date distributions were markedly different among years and seemed to influence the interannual recruitment strength of anchovy. We conclude that years characterized by juvenile survivors originating from the peak spawning period (May and June) would lead to considerable recruitment success. Downwelling events during the peak spawning period seem to affect larval survival. Furthermore, size‐dependent overwinter mortality would be an additional process that regulates recruitment strength in the anchovy population in the Bay of Biscay.  相似文献   

Litopenaeus vannamei (Boone) post-larval survival related to age, temperature, pH and ammonium concentration     

Francisco J Magallón Barajas  Rosalía Servín Villegas  Guillermo Portillo Clark  & Berenice López Moreno 《Aquaculture Research》2006,37(5):492-499

Transport of post‐larvae shrimp used in aquaculture is an important element of successful cultivation because of the potential for stress during stocking procedures. To find optimum transport conditions, several bioassays were performed in the laboratory to evaluate survival of whiteleg shrimp Litopenaeus vannamei 5–30‐day‐old postlarvae under conditions similar to those encountered during transport from the hatchery to nursery and shrimp ponds. Postlarvae were exposed for 4 h to different temperatures and pH levels ammonia concentrations. Survival was significantly reduced after a 4 h exposure to pH 9 and was inversely related to temperature with or without 7 mg L^?1 of ammonia. The 15‐ and 20‐day‐old postlarvae had higher survival rates than other ages. The lowest survival occurred in alkali conditions (pH 9), with 7 mg L^?1ammonia at 30 and 32°C. To assure optimal survival of postlarvae during transfer from the hatchery to the nursery and shrimp ponds, we recommend temperatures below 28°C, pH no higher than 8, no ammonia and post‐larval age at least 15 days.  相似文献   

The impact of thematic resolution on the patch-mosaic model of natural landscapes   总被引：1，自引：1，他引：0  

Guillermo Castilla  Kerry Larkin  Julia Linke  Geoffrey J. Hay 《Landscape Ecology》2009,24(1):15-23

We argue that thematic resolution, i.e., the level of categorical detail of a thematic map expressed by the number of classes included in the map legend, is an inherent component of the scale at which a landscape is analyzed. Changing the number of classes can change the configuration of the patch mosaic as much as changing the grain does. We address recent calls in this and other journals to deepen research in this topic. In particular, we report how thematic resolution affects the patchiness of mosaics representing natural landscapes, which have seldom been studied in this respect. We selected seven 50 × 50 km landscapes within national parks, each representative of a world biome. We applied an object-based unsupervised classification to Landsat TM imagery of these landscapes using increasing numbers of classes, between 2 and 50, and derived curves of mean patch size and patch density for each site. Our results are consistent with previous findings in that the patchiness of output mosaics increases monotonically with increasing thematic resolution, with a higher rate of increase up to eight classes that declines until it becomes roughly constant for more than 16 classes. However, this constant rate of increase is still considerable, meaning that, at least for natural landscapes, there is no threshold beyond which the patch-mosaic model is independent of the conceptual filter applied. This dependence on human fiat calls for re-thinking the patch-mosaic paradigm.  相似文献   

Comparing theoretical irrigation requirement and actual irrigation for citrus in Florida     

Consuelo C. Romero  Guillermo A. Baigorria 《Agricultural Water Management》2009,96(3):473-483

Florida ranks first in citrus production, with nearly 68% of all U.S. citrus growing in the season 2005-2006. Most of the citrus groves are located from central to south Florida, and agricultural irrigation permitting is regulated by three of Florida's five water management districts. Most of the permitting for citrus production in Highlands, Polk and Hillsborough counties is conducted by the Southwest Florida Water Management District (SWFWMD), and quantities are based on the District's AGMOD computer program. In 2003, the SWFWMD implemented new permit criteria so that permitted amounts were more representative of actual water use. This paper compares grower reported citrus irrigation water use in Highlands, Polk and Hillsborough counties from 1994 through 2005 with permitted and theoretical irrigation requirements calculated by a daily water balance. Two different sets of crop coefficients (K_c's) developed for citrus in Florida were compared in the daily soil water balance calculation of theoretical irrigation requirements. The percentage of irrigated area considered in this study ranged from 40 to 60% to simulate a range of grower practices. Meteorological data from two weather stations and additional rainfall information from 50 locations within the three counties was used in the water balance. Missing and error values in the meteorological historical record data were filled with weather generators. The multiannual average water consumption (including cold protection water use) from growers ranged from 243 (Hillsborough) to 406 mm (Highlands) and the multiannual average permitted irrigation requirement (without cold protection) ranged from 295 to 557 mm. The simulated gross irrigation requirements under different scenarios of location-K_c-wetted area were variable but mostly lower than the limits established by the district, except for some scenarios in Polk County, whose maximum simulated irrigation value reached 578 mm year⁻¹. In general, permitted limits recommended by the SWFWMD seem to be reasonable for the actual water use by growers in these counties.  相似文献