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l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis. 相似文献
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国产X65卷板在库鄯管道的应用 总被引：1，自引：0，他引：1
上海宝钢首次生产的Ｘ６５卷板，作为库鄯输油管道用钢哮，经制管和试验检查，钢材稳定、质量合格，完全符合ＡＰＩ５Ｌ标准，在力学性能和化学成分等方面邓国际水平，为我国高强度、高韧性的管 道用钢填补了空白。 相似文献
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运用二次回归正交旋转组合设计,在春播条件下研究黔苦2号苦荞籽粒产量与其播种量及N、P、K肥施用量间的关系.通过试验,建立了黔苦2号籽粒产量与主要农艺措施的数学模型,通过对模型的解析和模拟寻优分析.结果表明:黔苦2号籽粒产量高于2800kg/hm2的优化栽培技术方案:播种量67.14～74.93 kg/hm2,施N量28.88～40.12 kg/hm2,施P2O5量179.11～210.89 kg/hm2,施K2O量43.94～61.06kg/hm2;试验4因子对籽粒产量影响的程度大小为播种量>施K2O量>施P2O5量>施N量. 相似文献
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旨在利用覆盖全基因组和性状的特异性SNPs标记预测和牛、西门塔尔牛与荷斯坦牛杂种优势,为牛杂种优势利用和选种选配提供参考依据。本研究分别利用牛Illumina Bovine HD 770 K和GGP Bovine 100 K芯片对464头和牛、1 222头西门塔尔牛和43头荷斯坦牛3个亲本群体进行基因型分型,并通过牛QTLs数据库筛选与目的性状对应的QTLs,对比牛参考基因组映射得到与初生重、周岁重、胴体重性状相关的特异性SNPs;然后构建覆盖全基因组和性状特异性SNPs两种标记状态同源矩阵,通过计算杂交组合亲本间的遗传距离来预测品种间杂种优势,并利用配合力分析验证较优组合的实际杂交效果。结果表明,基于全基因组和性状特异性SNPs计算的各杂交组合遗传距离差异不显著。在全基因组水平上,西门塔尔牛♂×荷斯坦牛♀（S×H）与和牛♂×荷斯坦牛♀（W×H）亲本间杂交组合遗传距离分别为0.346 1和0.338 9;在初生重、周岁重和胴体重性状上,S×H亲本间遗传距离分别为0.343 1、0.348 7和0.336 7,而W×H遗传距离分别为0.337 6、0.340 7和0.329 2;两种SNPs标记计算的遗传距离均为S×H较大,W×H次之。因此,在初生重、周岁重、胴体重性状上,S×H为较优杂交组合。通过分析德系西门塔尔牛♂×荷斯坦牛♀实际杂交群体的配合力,发现10个父系在初生重性状上一般配合力和特殊配合力均为正效应,最高效应值分别达到3.760 9和8.931 2。西门塔尔牛与荷斯坦牛杂交可在初生重、周岁重和胴体重获得较高的杂种优势。 相似文献
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库鄯(库尔勒至鄯善)输油管道采用一系列国际标准进行工程设计,实现了高压力、大站距输送和常温密闭输油,解决了输送中的大落差问题,达到了全线的启停高度自动化,在劳动生产率、能耗、防腐质量、设备选用和通信水平等方面已经达到或超过了一些世界著名管道的水平。介绍了该条管道的技术特点并将其与国外同类输油管道进行了技术比较。 相似文献
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进口X65卷板制管后屈服强度明显下降，通过包申格效应试验，分析制管过程中的应力，应变对钢管屈服强度的影响，对进口卷板制管前后屈服强度的变化情况进行了详细研究，指出了影响钢管强度的几个主要因素。 相似文献
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分析了螺旋埋弧焊钢管生产中钢卷拆卷矫平、预弯成型过程中的正反受力变形。由于多晶体金属中部分晶粒的微塑性变形以及铁素体体心立方晶体受力后的位错反应、位错塞积或位错分解产生第二类残余内应力，用第二类残余内应力对包申格效应的产生机理进行了阐述说明。论述了包申格效应对钢管屈服值的影响。 相似文献
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美国ＮＵＣＯＲ钢厂热轧板卷首次在国内卷管中应用，为了获得第一手资料，对其首批进口的板卷抽样进行了化学成分分析、力学性能试验和卷管后的爆破试验，分析结果表明，该厂生产的Ｘ６５热轧板卷（首批进口）的各项性能均符合ＡＰＩ标准规定和意大利斯南普吉提公司的设计要求。卷管将使用于库鄯输油管道。 相似文献
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Fuller W Bazer Gwonhwa Song Jinyoung Kim Kathrin A Dunlap Michael Carey Satterfield Gregory A Johnson Robert C Burghardt 《畜牧与生物技术杂志(英文版)》2012,3(3):109-129
There is a dialogue between the developing conceptus (embryo-fetus and associated placental membranes) and maternal uterus which must be established during the peri-implantation period for pregnancy recognition signaling, implantation, regulation of gene expression by uterine epithelial and stromal cells, placentation and exchange of nutrients and gases. The uterus provide a microenvironment in which molecules secreted by uterine epithelia or transported into the uterine lumen represent histotroph required for growth and development of the conceptus and receptivity of the uterus to implantation. Pregnancy recognition signaling mechanisms sustain the functional lifespan of the corpora lutea (CL) which produce progesterone, the hormone of pregnancy essential for uterine functions that support implantation and placentation required for a successful outcome of pregnancy. It is within the peri-implantation period that most embryonic deaths occur due to deficiencies attributed to uterine functions or failure of the conceptus to develop appropriately, signal pregnancy recognition and/or undergo implantation and placentation. With proper placentation, the fetal fluids and fetal membranes each have unique functions to ensure hematotrophic and histotrophic nutrition in support of growth and development of the fetus. The endocrine status of the pregnant female and her nutritional status are critical for successful establishment and maintenance of pregnancy. This review addresses the complexity of key mechanisms that are characteristic of successful reproduction in sheep and pigs and gaps in knowledge that must be the subject of research in order to enhance fertility and reproductive health of livestock species. 相似文献