排序方式： 共有18条查询结果，搜索用时 31 毫秒
1.
Yasunaga YOSHIKAWA Masami MORIMATSU Kazuhiko OCHIAI Toshina ISHIGURO-OONUMA Ryo MORIOKA Kento OKUDA Koichi ORINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):759
Mammary tumors are the most common tumors in women and non-spayed female dogs. One of the reasons for mammary tumors is mutations of the tumor suppressor gene, BRCA2. BRCA2 participates in homologous recombination repair by interacting with the RAD51 recombinase. BRCA2 has two RAD51-binding domains, consisting of BRC repeats and the C-terminal RAD51-binding domain, respectively. Although several studies have addressed the function of the C-terminal RAD51-binding domain of human BRCA2, the amino acid sequences required for the RAD51-interaction activity remain unclear. In this study, the C-terminal RAD51-binding domains of canine and human BRCA2 were compared; the canine domain displayed a weaker interaction with RAD51. This difference was attributed to the C-terminal portion of the domain via a comparison between canine and human domains. Furthermore, peptides shorter than those previously reported displayed RAD51-interacting activity, and a core motif of this domain consisting of 25 amino acids was identified. Since a mutation (S3323N) was reported in the core motif of this domain, the effect of this mutation was evaluated. The mutant exhibited similar RAD51-binding activity as that of the wild-type protein, suggesting that the mutation was functionally neutral. These data suggested that the C-terminal portion of the BRCA2 C-terminal RAD51-binding domain influenced its RAD51-interaction activity, and a minimum core motif of 25 amino acids was identified in this domain. These data may help clarify BRCA2 function, as well as the tumorigenic effects of BRCA2 mutation. 相似文献
2.
Mizuki OGAWA Yasutomo HORI Nobuyuki KANNO Naoki IWASA Takeshi TOYOFUKU Noriko ISAYAMA Akane YOSHIKAWA Ryota AKABANE Atsushi SAKATANI Hirosumi MIYAKAWA Huai-Hsun HSU Yuichi MIYAGAWA Naoyuki TAKEMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):705
Plasma N-terminal pro-atrial natriuretic peptide (NT-proANP) concentration increases with progression of myxomatous mitral valve disease (MMVD) in dogs. This multicentre, prospective study compared plasma NT-proANP, N-terminal pro-brain natriuretic peptide (NT-proBNP), ANP, and cardiac troponin I (cTnI) concentrations in dogs with MMVD for their characteristics and discriminatory ability to detect cardiac dilatation and congestive heart failure (CHF). Thirty-six healthy dogs and 69 dogs with MMVD were included. Clinical variables were obtained via physical examination, thoracic radiography, and echocardiography. The discriminatory ability of each cardiac biomarker (CB) to determine the presence or absence of cardiac dilatation (event 1) and CHF (event 2) was evaluated using the receiver operating characteristic curves. Plasma NT-proANP, NT-proBNP, and ANP concentrations showed a significant association with the left atrium/aorta ratio (P<0.01). The area under the curve of plasma NT-proANP and NT-proBNP concentrations were 0.72 and 0.75, respectively in event1 and 0.72 and 0.76, respectively in event2. Plasma NT-proANP and NT-proBNP concentrations showed sensitivity 80.0 and 80.0%; specificity 67.6 and 64.7% in event1 (cutoff value; 8,497.81 pg/ml and 1,453.00 pmol/l, respectively) and sensitivity 85.7 and 81.0%; specificity 60.4 and 64.6% in event2 (cutoff value; 8,684.33 pg/ml and 1,772.00 pmol/l, respectively). In dogs with MMVD, plasma NT-proANP, NT-proBNP, and ANP concentrations increase with left atrial enlargement. Particularly, plasma NT-proANP and NT-proBNP concentrations appeared to be equally useful in the discriminatory ability to detect cardiac dilatation and CHF. 相似文献
3.
HIROTO MURASE TSUTOMU TAMURA HIROSHI KIWADA YOSHIHIRO FUJISE HIKARU WATANABE HIROSHI OHIZUMI SHIROH YONEZAKI HIROSHI OKAMURA SHIGEYUKI KAWAHARA 《Fisheries Oceanography》2007,16(2):186-201
A study of common minke and Bryde's whales was conducted in the western North Pacific in the 2000 and 2001 summer seasons to estimate prey selection of cetaceans as this is an important parameter in ecosystem models. Whale sighting and sampling surveys and prey surveys using quantitative echosounder and mid‐water trawl were carried out concurrently in the study. Biomasses of Japanese anchovy, walleye pollock and krill, which were major prey species of common minke and Bryde's whales, were estimated using an echosounder. The results suggested that common minke whale showed prey selection for Japanese anchovy while they seemed to avoid krill in both the offshore and coastal regions and walleye pollock in the continental shelf region. Selection for shoaling pelagic fish was similar to that in the eastern North Atlantic. Bryde's whale showed selection for Japanese anchovy in August 2000 and July 2001, while it showed prey selection for krill in May and June in 2001. 相似文献
4.
本研究以黑河下游额济纳旗成熟的河岸胡杨林为对象,分析不同年龄胡杨(Populus euphratica)的年轮宽度序列与不同时间尺度环境因子的关系。结果表明:①在年尺度上,气温是影响胡杨径向生长的主要因子,但不同年龄胡杨对气温的响应有所不同。②在季节尺度上,休眠季气温越高越有利于壮龄胡杨的径向生长,而生长季气温越高越不利于老龄胡杨的径向生长。③在月尺度上,前一年3月和当年9月的平均气温越高,对壮龄胡杨的径向生长越有利,而当年10月平均气温越高,对老龄胡杨的径向生长越不利;前一年7月径流量的增加对老龄胡杨的径向生长有利,而当年2月径流量的增加对壮龄胡杨径向生长有利。综上所述,在季节和年等相对较长的时间尺度上,气温是影响胡杨径向生长量的主要因素,随着时间尺度的缩小,胡杨对环境因子的响应更为细化,并且表现出一定的滞后效应。径流量作为重要的环境因子在月尺度上才突显出来,前一年7月和当年2月的径流补给对胡杨径向生长有极大的促进作用。因此,在黑河统一调度管理过程中,可通过适度调增2月和7月洪水的下泄量来满足胡杨径向生长对水分的需求。 相似文献
5.
MONIQUE N. MAYER HIROTO YOSHIKAWA NARINDER SIDHU 《Veterinary radiology & ultrasound》2009,50(3):319-322
We quantified the effect of tissue inhomogeneity on dose distribution in a canine distal extremity resulting from treatment with cobalt photons and photons from a 6 MV accelerator. Monitor units for a typical distal extremity treatment were calculated by two methods, using equally weighted, parallel-opposed fields. The first method was a computed tomography (CT)-based, computerized treatment plan, calculated without inhomogeneity correction. The second method was a manual point dose calculation to the isocenter. A computerized planning system was then used to assess the dose distribution achieved by these two methods when tissue inhomogeneity was taken into account. For cobalt photons, the median percentage of the planning target volume (PTV) that received <95% of the prescribed dose was 4.5% for the CT-based treatment plan, and 26.2% for the manually calculated plan. For 6 MV photons, the median percentage of the PTV that received <95% of the prescribed dose was <1% for both planning methods. The PTV dose achieved without using inhomogeneity correction for cobalt photons results in potentially significant under dosing of portions of the PTV. 相似文献
6.
Summary. The herbicidal activity on Echinochloa crus-galli (L.) Beauv. var. oryzicola Ohwi of the photosynthesis inhibitors chlorbromuron and simetryne decreased rapidly with increasing plant age. The decreased activity is explained on the basis of a proposed ‘growth-dilution’ hypothesis, which is also relevant to the synergism observed with combinations of these two herbicides and various growth inhibitors. In an attempt to make use of the synergistic action for practical weed control in paddy rice (Oryza sativa L.) a test was made to determine the effects on crop and weeds of granular formulations of two herbicide combinations, namely 5% w/w 2-(2-chlorobenzylthio)-5-propyl-l,3,4-oxadia-zole (benoxazole) plus l-5% w/w of either chlorbromuron or simetryne. Both formulations were applied at doses of 30 and 40 kg product/ha and the benoxazole+ simetryne mixture gave very promising results. Interactions synergiques entre les inhibiteurs de croissance et la photosynthése I. L'hypothése du rolentissement de la croissance Résumé. L'action herbicide sur Echinochloa crus-galli (L.) Beauv. var. oryzicola Ohwi d'inhibiteurs de la photosynthése, tels que le chlorbromuron et la simétryne, décroît rapidement à mesure que l'age des plantes augmente. Cette diminution d'activité a été expliquée en proposant une hypothise basée sur le ralentissement de la croissance, hypothése qui s'appuie également sur le synergisme observé avec les combinaisons de ces deux herbicides et divers inhibiteurs de croissance. En vue d'utiliser l'action synergique dans la pratique pour le désherbage du riz (Oryza sativa L.), un essai a été réalisé pour determiner les effets, sur la récolte et sur les mauvaises herbes, de formulations des deux herbicides associés, comprenant 5% (en poids) de 2-(2-chlorobenzylthio)-5- propyle-l,3,4-oxadiazole (bénoxazole) plus 1,5% (en poids), soit de chlorbromuron, soit de simétryne. Les deux formulations ont été appliquées a des doses de 30 et 40 kg/ha et le mélange bénoxazole+simétryne a donné des résultats trés prometteurs. Synergistische Wechselmrkungen zwischen Wachstums- und Photosynthesehemmstoffen. I. Die Theorie des ‘Verdünnungseffektes durch Wachstum” Zusammenfassung. Die herbizide Wirkung der Photosynthesehemmstoffe Chlorbromuron und Simetryn gegenüber Echinochloa crus-galli (L.) Beauv. var. oryzicola Ohwi nahm mit zunehmendem Alter der Pflanzen schnell ab. Dies wurde mit einer Theorie der ‘Verdünnungswirkung durch Wachstum’ erklärt, die ausserdem die Begründung für den Synergismus lieferte, den man bei Kombination dieser zwei Herbizide mit verschiedenen Wachstumshemmstoffen beobachtete. Um diese synergistische Wirkung für die praktische Unkrautbekämpfung in Reis (Oryza sativa L.) nutzbar zu machen, wurde ein Versuch angelegt. Damit sollte die Wirkung granulierter Formulierungen zweier Herbizidkombinationen, nämlich 5% w/w 2-(2-Chlorbenzylthio)-5-propyl- 1,3,4-oxadiazol (Benoxazol) plus 1,5% w/w Chlorbromuron oder Simetryn, auf Kultur früchte und Unkräuter ermittelt werden. Beide Formulierungen wurden mit 30 und 40 kg/ha angewandt. Die Benoxazol-Simetryn-Mischung ergab dabei vielversprechende Ergebnisse. 相似文献
7.
Hiroshi OKAMOTO Mamoru SATO Yoshio MIYATA Masami YOSHIKAWA Makoto ISAKA 《Journal of General Plant Pathology》2000,66(1):86-94
Bacterial strains isolated from the rhizosphere of angelica trees were evaluated for their antagonistic activity against Phytophthora cactorum, a causal agent of Phytophthora root rot. Of these, three bacterial strains, designated as T-1-8, T-1-14 and T-1-23, strongly inhibited mycelial growth of
P. cactorum ARE-862 in a dual-culture plate assay. Biocontrol activity of these strains was then examined by dipping root of young seedlings
of angelica trees into a bacterial suspension. The incidence of Phytophthora root rot was markedly suppressed for at least 79 days in pot tests when treated seedlings were planted in naturally infested
soil. The suppression was maintained through June of the next year. In addition, these strains significantly reduced the development
of Phytophthora root rot up to 47 days in naturally infested field and up to 63 days (the last day of testing) in an artificially (moderately)
infested field. Based on their main bacteriological properties, strain T-1-14 was identified as Enterobacter cloacae and T-1-8 and T-1-23 were identified as Serratia ficaria.
Received 5 July 1999/ Accepted in revised form 25 October 1999 相似文献
8.
KENSUKE NAKAMURA NOBORU SASAKI MUNEYOSHI YOSHIKAWA HIROSHI OHTA SHIANG-JYI HWANG TAKAHIRO MIMURA MASAHIRO YAMASAKI MITSUYOSHI TAKIGUCHI 《Veterinary radiology & ultrasound》2009,50(1):104-108
Contrast-enhanced ultrasonography has an important role in the detection of tumors in humans. The second-generation contrast agent Sonazoid has the ability of real-time contrast imaging along with parenchymal imaging. The purposes of this study were to determine the effect and duration of Sonazoid on the changes in gray-scale enhancement of canine spleen and to establish an appropriate protocol for contrast-enhanced ultrasonography of canine spleen. Six healthy beagles were injected with an intravenous bolus of Sonazoid. In the spleen parenchyma, the enhancement was maintained up to 30 min after injection. Moreover, for 5–22 s after injection, gray-scale enhancement of splenic arteries afforded arterial imaging. Perfusion of the kidney may be investigated from 3.6 s to 3.5 min after injection of Sonazoid. These results suggest that Sonazoid is applicable to canine spleen parenchymal imaging and that the optimal time for the parenchymal imaging is 7–30 min after injection. The findings of this quantitative study should prove useful in the evaluation of diffuse or focal splenic and renal diseases in dogs. 相似文献
9.
10.