Inactivation of the fungus Aspergillus fumigatus     

H Geissler  M Weingarten  H M Hafez 《DTW. Deutsche tier?rztliche Wochenschrift》1986,93(1):23-24

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Serological evidence of the occurrence of bluetongue in Iraq.     

S M Hafez  E G Pollis  S A Mustafa 《Tropical animal health and production》1978,10(2):95-98

Precipitating antibodies against bluetongue were detected in sheep and goat serum samples collected from animals slaughtered in Baghdad abattoir. Out of 294 sheep serum samples and 110 goat serum samples examined, 28 and 18 samples respectively showed precipitating activity. In addition, examination of sheep serum samples collected from localities where clinical cases similar to bluetongue were previously reported revealed the presence of bluetongue precipitating antibodies in 101 sera out of 198 samples examined. This is the first report confirming the occurrence of bluetongue in Iraq.  相似文献   

Effect of coated plant extracts on Histomonas meleagridis and growth of bacteria in vitro   总被引：1，自引：0，他引：1  

Hauck R  Hafez HM 《Avian diseases》2007,51(4):880-883

Three coated plant extracts (RepaXol and two experimental formulations) were tested for their minimal lethal concentration for histomonads in vitro and the effect of those substances on the bacterial growth in the histomonadal culture. After 48 hr, RepaXol and experimental formulation B were lethal to histomonads at a concentration of 1.25 microl/ml. Experimental formulation C was lethal at a concentration of 2.5 microl/ml. All products also decreased the growth of bacteria at concentrations inhibiting the growth of histomonads.  相似文献   

Serological investigations on reticuloendotheliosis in turkey flocks     

Hafez HM 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(7):547-550

Four meat turkey and one turkey breeding flocks were surveyed for antibodies against reticuloendotheliosis virus (REV) at different intervals using commercial enzyme-linked immunosorbent assays. In addition, serum samples collected from 18 flocks at different ages were also tested for antibodies against REV. No antibodies were detected in any of the four meat turkey flocks that were surveyed. In the breeder flock, 20%) of tested samples from 1-day-old poults were positive. Between the fourth and 12th weeks all samples that were tested yielded negative results. At 16 weeks of age 15% of samples yielded a positive reaction, but antibodies could not be detected 4 weeks later. Examination of serum samples from 18 different flocks at various ages revealed that antibodies could be detected in five flocks. The percentage of positive sera per flock ranged between 10 and 40%.  相似文献   

Investigations on different Ornithobacterium rhinotracheale "ORT" isolates   总被引：1，自引：0，他引：1  

Hafez HM  Sting R 《Avian diseases》1999,43(1):1-7

The aim of the present investigation was to determine the antigenic relationship between different Ornithobacterium rhinotracheale (ORT) isolates and to serotype field isolates obtained from turkey and chickens. Different antigen extractions (heat-stable, proteinase K-stable [lipopolysaccharide], and sodium dodecyl sulfate [SDS] extractions) were prepared from each serotype (A, B, C, D, E, and G) as well as from 21 ORT field isolates and examined in agar gel precipitation (AGP) and enzyme-linked immunosorbent assay (ELISA) tests. The field isolates were cultured from turkey (16 isolates) and chicken (5 isolates) flocks showing respiratory manifestations. Monospecific reactions were obtained with heat-stable as well as proteinase K-stable antigens prepared from serotypes A, C, D, E, and G in AGP tests. On the other hand, with the same antigen preparations from a strain of serotype B in AGP tests, cross-reactions with antisera prepared against serotypes A and E could be detected. The cross-reactions were observed mostly between 48 and 72 hr. In applications of SDS-antigen preparations in AGP tests, cross-reactions between all serotypes except serotype C were detected between 24 and 72 hr. Testing all antigen preparation in ELISA, different cross-reactions were observed and the evaluation of the results is very difficult. Serotyping of the field isolates in AGP tests by using heat-extracted antigens showed after 24 hr that 10 out of 16 isolates from turkey belonged to serotype B, five to serotype A, and one to serotype E. Results obtained after 48-72 hr revealed cross-reactions between serotype B and E in 11 cases and between A and B in two cases. All five isolates obtained from chicken reacted after 24 hr only with serum against serotype A. After 48-72 hr, two isolates showed cross-reaction with antiserum against serotype B. Similar results were obtained with proteinase K-stable antigen.  相似文献   

Effect of sample selection from experimentally contaminated hatching eggs and freshly hatched chicks on Salmonella enteritidis detection rate.     

H M Hafez  S Jodas 《DTW. Deutsche tier?rztliche Wochenschrift》1992,99(12):489-490

The conventional culture methods of hatching eggs using shell and/or egg contents for detection of salmonella organisms give mostly unsatisfactory results. The aim of the present study is to evaluate selection of other samples and techniques of culturing hatching eggs and freshly hatched chicks. This current study provides the best evidence of Salmonella enteritidis in artificially contaminated eggs (Layer type) by using enrichment broth in empty egg shell samples in comparison to culturing samples from yolk, albumen or from shell above the air cell (with the outer shell membrane). The isolation rates could be enhanced if empty egg shell was initially filled with Buffered Pepton Water as pre-enrichment broth. Examination of organs from freshly hatched chicks revealed that crop samples give mostly higher reisolation rates.  相似文献   

Isolation of parvovirus from kidney cell cultures of gnotobiotic piglets.     

S M Hafez  B Liess 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1979,26(10):820-827

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Sialic acid and some physicochemical characteristics of bovine cervical mucus     

K Hamana  A A el-Banna  E S Hafez 《The Cornell veterinarian》1971,61(1):104-113

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火鸡疫病:对兽医的新挑战     

张培君 《中国家禽》2007,29(19):8-11

现代火鸡业,定位于产量高、品质好、价格低,且要与消费者对禽肉产品的需求相一致,预防火鸡疫病以保障人类健康是现代火鸡业的导向性目标.……  相似文献   

Swollen head syndrome: clinical observations and serological examinations in West Germany     

H M Hafez  U L?hren 《DTW. Deutsche tier?rztliche Wochenschrift》1990,97(8):322-324

Swollen Head Syndrome (SHS) affects both broiler and broiler breeders and has become a problem in many countries. Clinical signs similar to those described in SHS conditions have been observed in northern Germany with the disease observed mostly in chicken broilers between 4 and 5 weeks of age and in broiler breeders between 24 and 36 weeks of age. The mortality rate in broilers has been variable from negligible to 5%. In broiler breeders, the symptoms were accompanied with a drop in egg production reaching 2-3% after 1 to 2 weeks. 422 Serum samples from 21 broiler flocks were examined for the presence of antibodies to TRT-virus using indirect ELISA tests. Antibodies were detected in 2 flocks which suffered from SHS, but not in 2 flocks showing respiratory manifestations nor in 17 apparently healthy flocks. The results obtained from testing 178 serum samples from 10 breeder flocks revealed that antibodies to TRT-virus were present in one flock with SHS signs as well as in 6 apparently healthy flocks. In addition, no antibodies were found in sera collected from 3 flocks--one with SHS and respiratory disease history; one recovered from respiratory infection and one without disease history. Examination of paired serum samples from 3 breeder flocks that suffered from typical SHS-signs collected at the onset of the disease and during the convalescent stage, as well as sera from one broiler and one breeder flocks that showed respiratory manifestations, showed that there are significant increases in the number of positive sera in the flocks with SHS symptoms. The other flocks remained free from TRT antibodies.  相似文献