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Zusammenfassung Erstmalig wurden mit den vorliegenden Untersuchungen Spargelstangen zur Haupterntezeit auf endophytischen Pilzbefall untersucht. Sie zeigen, dass im Ernteprodukt zwar Fusarium proliferatum als potenzieller Mykotoxinbildner zu finden ist. Eine mögliche natürliche Kontamination mit Fumonisinen bestätigte sich nicht. Von den mit F. proliferatum infizierten Stangen wies nur eine Stange mit grau-rosa-orange farbenen Gewebeveränderungen an der Basis sichtbare Symptome auf. Allgemeine Rückschlüsse auf eine mögliche Gefährdung oder Nichtgefährdung des Verbrauchers beim Verzehr von mit F. proliferatum kontaminierten, symptomlosen Stangen können aus der Analyse nicht gezogen werden. Hierzu müssen weitergehende Untersuchungen zur Wirt-Pathogen-Interaktion erfolgen und die phänotypischen und genotypischen Einflussfaktoren in diesem Prozess noch näher untersucht werden. 相似文献
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Photoperiodism and effects of indoleamines in a unicellular alga, Gonyaulax polyedra 总被引:3,自引:0,他引:3
Mediation of photoperiodic effects by indoleamines, especially melatonin, is known in higher vertebrates. A similar mechanism may occur in a unicellular alga, the dinoflagellate Gonyaulax polyedra. This organism entered the dormant stage of a cyst upon short-day treatment at lowered temperatures. Interruption of darkness by 2 hours of light prevented cyst formation, even when the overall duration of light was the same as in cyst-inducing short days. When given in a noninducing photoperiod, melatonin and an analog, 5-methoxytryptamine, substances that had previously been shown to occur in Gonyaulax, provoked cyst formation. Methoxylated indoleamines may play a role as mediators of darkness in this unicellular, in a similar way as in vertebrates, suggesting a common biochemical basis of photoperiodism. 相似文献
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I Balzer C Bogdani? S Pepeljnjak 《Journal of the Association of Official Analytical Chemists》1978,61(3):584-585
A multimycotoxin thin layer chromatographic method is described for the analysis of corn. Aflatoxins are extracted from the samples with acetonitrile-water, and sodium bicarbonate is added to separate the acidic ochratoxin from zearalenone and aflatoxin B1. After chloroform extraction, 1N NaOH is added to separate zearalenone and aflatoxin B1. The separated mycotoxins are spotted on TLC plates, which are then examined under ultraviolet light. The following recoveries (%) were obtained for corn samples: aflatoxin B1 71, ochratoxin A 87, and zearalenone 85. The limits of detection for the respective mycotoxins were 2, 40, and 200 ppb. 相似文献
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The aim of this prospective study was to evaluate the prevalence of feline haemotropic mycoplasmas in Germany, to determine probable risk factors for these infections and to compare the diagnostic value of microscopic examination of blood smears to polymerase chain reaction (PCR). For the prevalence study, convenience samples (Ethylene diamine-tetraacetic acid (EDTA) blood) from 262 (64.5% male and 35.5% female) cats were included. A PCR for the detection of Mycoplasma haemofelis (MHF) and 'Candidatus Mycoplasma haemominutum' (CMH) as well as a feline leukaemia virus (FeLV)/feline immunodeficiency virus (FIV) enzyme-linked immunoassay was performed. Blood smears from 224 cats were examined and the sensitivity and specificity of the microscopic diagnosis were determined. The prevalence of CMH, MHF, and CMH/MHF co-infection was 22.5%, 4.5%, and 0.8%, respectively. CMH was significantly associated with male gender (P=0.047), older age (P=0.0015) and both FeLV (P=0.002) and FIV infections (P<0.0001). However, there was no association between the presence of anaemia and CMH/MHF infection. The respective sensitivity and specificity of the microscopic diagnosis were 10.3% and 87.1% for a CMH infection and 0.0% and 98.0% for MHF infection. 相似文献
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Emergence of cutaneous neosporosis in a dog receiving immunosuppressive therapy: molecular identification and management
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