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Chiara De Lucchi Piergiorgio Stevanato Linda Hanson Mitch McGrath Lee Panella Marco De Biaggi Chiara Broccanello Marco Bertaggia Luca Sella Giuseppe Concheri 《Euphytica》2017,213(3):71
Fusarium spp. cause severe damage in many agricultural crops, including sugar beet, with Fusarium oxysporum historically being considered as the most damaging of all species. Sugar beet needs to be protected from this class of soil-borne pathogens in order to ensure an optimal sugar yield in the field. Genetic control of the disease is crucial in managing these pathogens. Identification of single nucleotide polymorphism (SNP) markers linked to resistance can be a powerful tool for the introgression of valuable genes needed to develop Fusarium-resistant varieties. A candidate gene approach was carried out to identify SNP markers linked to putative Fusarium resistance sources in sugar beet. Five resistant analogue genes (RGAs) were screened by means of high resolution melting (HRM) analysis in a set of sugar beet lines, considered as resistant and susceptible to Fusarium oxysporum. HRM polymorphisms were observed in 80% of amplicons. Two HRM polymorphisms were significantly associated with Fusarium resistance (P < 0.05). The amplicons that showed association were sequenced and two SNPs were identified. The association was further validated on 96 susceptible and 96 resistant plants using competitive allele-specific PCR (KASPar) technology. The selected SNPs could be used for marker-assisted breeding of Fusarium resistance in sugar beet. 相似文献
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S. Zetterström R. Hanson A. Velloso Alvarez F. Caldwell L. Boone J. Schumacher 《Equine Veterinary Education》2021,33(12):e482-e489
Conidiobolus coronatus is one of the most commonly identified upper respiratory fungal pathogens in horses. This article includes a review of clinical signs, diagnostics, treatment and outcomes in previously reported cases of equine conidiobolomycosis, as well as six additional cases seen at our hospital. Each of the six horses presented with a complaint of serosanguinous or haemorrhagic nasal discharge and conidiobolomycosis was confirmed by histopathology and fungal culture. Five horses recovered after administration of antifungal drugs alone (n = 4) or in combination with extensive nasal septum resection (n = 1). Surgical treatment alone was ineffective. One horse was euthanised without treatment because of the extent of the disease. 相似文献
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Strategies for reducing subsurface drainage in irrigated agriculture through improved irrigation 总被引：1，自引：0，他引：1
The traditional approach ofinstalling subsurface drainage systems tosolve shallow ground water problems is notfeasible along the west side of the SanJoaquin Valley of California because of thelack of drain water disposal methods thatare economical, technically feasible, andenvironmentally friendly. Thus, optionssuch as drainage reduction through improvedirrigation and drain water reuse are beingexamined as methods for coping with thesubsurface drainage problem. This paperdiscusses options for reducing subsurfacedrainage through improved irrigationpractices. Options are discussed forimproving irrigation system design such asupgrading existing irrigation methods andconverting to systems with higher potentialirrigation efficiencies. Methods forimproving water management are alsopresented. Case studies on upgradingexisting systems or converting to otherirrigation methods are presented along with study results of the effect of variouspolicies on reducing subsurface drainage. 相似文献
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ABSTRACT Geminiviruses are a group of single-stranded DNA viruses that cause major losses on a number of important crops throughout the world. Bean golden mosaic virus (BGMV) is a typical bipartite, whitefly-transmitted geminivirus that causes a severe disease on beans (Phaseolus vulgaris) in the Western Hemisphere. The lack of natural resistance to geminiviruses has led to attempts to engineer resistance, particularly through the use of pathogen-derived resistance strategies. The rep gene contains several conserved domains including nucleoside triphosphate (NTP)-binding and DNA-nicking domains and is the only geminiviral gene necessary for replication. Previous analysis by our group and others has demonstrated that the NTP-binding and DNA-nicking domains are necessary for geminiviral DNA replication. The ability of the rep gene and rep gene mutants to interfere with geminiviral DNA replication, when expressed in trans, was examined using a transient assay in a tobacco suspension cell culture system. Wild-type (wt) and mutant rep genes were cloned into plasmids under the control of the cauliflower mosaic virus 35S promoter for in planta expression and coinoculated into tobacco cells with infectious clones of various geminiviruses. The wt rep gene from BGMV-GA was able to support replication of BGMV-GA DNA-B. Several different rep gene mutants, with function-abolishing mutations in the NTP-binding or DNA-nicking domains, were potent trans-dominant inhibitors of geminiviral DNA replication. 相似文献
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