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Rafiqul Islam Harendra Kumar Gyanendra Singh Binsila B. Krishnan Sahadeb Dey 《Veterinary research communications》2017,41(3):201-209
The study was planned to see if there is any important and significant changes in the PMN function in cows suffering from postpartum reproductive diseases (PRD). Blood sampling was done from 41 pregnant cows on 15 days prepartum (?15d), calving day (0d), 15 days (15d) and 30 days (30d) postpartum and thorough gynaecological examination was performed on 0d, 15d, 30d and 45d for diagnosis of PRD like retained placenta (RP), clinical metritis (CM), clinical endometritis (CE) and delayed involution of uterus (DIU). The heparinised blood was used for isolation of PMN leukocytes for estimation of superoxide (SO), hydrogen peroxide (H2O2) and enzyme myeloperoxidase (MPO) activity in each group of cows. The SO production (ΔOD) was greater for normal (0.19 ± 0.05) than cows suffering from RP (?0.12 ± 0.09), CM (?0.15 ± 0.13) and CE (?0.07 ± 0.05) at -15d. The mean value was greater for normal cows (0.12) than the cows with PRD (0.05 to 0.9) at 30d. The H2O2 production was greater for normal than cows with PRD at all sampling days and significantly greater than cows with RP and CE at 15d (p < 0.01) and 30d (P < 0.05). The MPO activity (μmol/1 × 107) was greater for normal (18.77 ± 1.27) than for RP (12.52 ± 2.57) and CM (11.31 ± 3.30) cows on 0d. The depressed capability of the PMN from the cows with PRD to produce SO, H2O2 and MPO during the periparturient period indicated their association with the development of RP, CM and CE. 相似文献
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Kharayat Nitish Singh Sharma G Chethan Kumar Gandham Ravi Bisht Deepika Chaudhary Gangaram Singh Sanjay Kumar Das Gautum Kumar Garg Anil Kumar Kumar Harendra Krishnaswamy Narayanan 《Veterinary research communications》2019,43(4):261-269
Veterinary Research Communications - Toll like receptors (TLRs) and β-defensins expressed in the endometrium are part of the innate uterine defense mechanism (UDM). In the present study,... 相似文献
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Nanjundan Joghee Aravind Jayaraman Radhamani Jalli Singh Kunwar Harendra Kumar Arun Thakur Ajay Kumar Singh Karnal Meena Kailash Narayan Tyagi Rishi Kumar Singh Dhiraj 《Genetic Resources and Crop Evolution》2022,69(1):145-162
Genetic Resources and Crop Evolution - Indian mustard [Brassica juncea (L.) Czern.] is a major edible oil crop of India. The Indian Council of Agricultural Research—National Bureau of Plant... 相似文献
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Megha Pande Goutam Kumar Das Firdous Ahmad Khan Mihir Sarkar Mohan Chandra Pathak Jai Kishan Prasad Harendra Kumar 《Tropical animal health and production》2013,45(3):805-810
A vast majority of the world buffalo resource is concentrated in tropical and subtropical countries. Apart from heat stress and poor nutritional availability, endometritis is one of the most commonly encountered reproductive problems limiting fertility and consequently productive potential of the species. As demonstrated recently, endometritis impairs growth and follicular fluid composition of the largest follicle in buffalo. In the present study, the effect of endometritis on luteal development, function, nitric oxide (NO), and ascorbic acid was investigated. Reproductive tracts were collected from 90 cyclic buffaloes at an abattoir and grouped into endometritic (n?=?36) or non-endometritic (n?=?54) buffaloes based on physical examination of uterine mucus, white side test, and uterine cytology. Samples with pus-containing mucus, positive reaction on white side test, and/or >5 % neutrophils were considered to be positive for endometritis. Corpora lutea were enucleated, weighed, classified into stages I to IV, and assayed for progesterone (P4), NO, and ascorbic acid concentrations. Endometritic buffaloes had lesser (P?<?0.0001) luteal weight and P4, NO, and ascorbic acid concentrations than non-endometritic buffaloes. The findings indicated that endometritis impairs corpus luteum development and function in buffalo. Reduced luteal NO and ascorbic acid concentrations during endometritis are novel findings. 相似文献
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A number of 2-aryl- and 2-aryloxymethyl-5-aryl-7H-1,3,4-thiadiazolo[3,2-a][1,3,5]triazine-7-thiones have been synthesised and their fungitoxicities determined in vitro against Aspergillus niger and Fusarium oxysporum. Two alternative routes for synthesis of the title compounds are described. Some of the compounds had activities comparable with that of the commercial fungicide mancozeb. Structure/activity relationships for the compounds are discussed. 相似文献
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Evaluation of genetic relationship among six Labeo species using randomly amplified polymorphic DNA (RAPD) 总被引：1，自引：0，他引：1
Paramananda Das Harendra Prasad Prem Kumar Meher Ashoktaru Barat & Ranjit Kumar Jana 《Aquaculture Research》2005,36(6):564-569
Labeo species constitute an important group of fish with intense diversity and potential for commercial aquaculture in many Southeast Asian nations including the Indian subcontinent. The present investigation involves the comparative analysis of randomly amplified polymorphic DNA (RAPD) profiles of six Labeo species viz., L. bata (bata), L. calbasu (calbasu), L. dyocheilus (dyocheilus), L. fimbriatus (fimbriatus), L. gonius (gonius) and L. rohita (rohu) at the nuclear DNA variation level. Fifteen decamer random primers were chosen from 40, which amplified a total of 449 DNA fragments ranging in size from 400 to 3000 bp. Both monomorphic and polymorphic DNA bands were identified based on their presence or absence that could be used for specieswise differentiation. Similarity coefficients were calculated to quantify the genetic variation within and between species. On an average, the highest intra‐species genetic similarity value was found in calbasu (0.93) followed by rohu and fimbriatus (0.91), bata (0.87), gonius (0.86) and dyocheilus (0.77). The interspecies genetic similarity estimates among the species of Labeo were used to deduce their phylogenetic relationships. The cluster analysis showed two main clusters, one with calbasu, rohu, fimbriatus and gonius and another with bata and dyocheilus. The study provides evidence that RAPD could be used for genetic differentiation of closely related species. 相似文献
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Dushyant Yadav Amit Kumar Singh Brijesh Kumar Ajit Singh Mahla Sanjay Kumar Singh Manas Kumar Patra Harendra Kumar Sachin Kumar Bhawna Tyagi Med Ram Verma Narayanan Krishnaswamy 《Reproduction in domestic animals》2019,54(12):1651-1659
We have shown that dietary supplementation of n‐3 polyunsaturated fatty acid (n‐3 PUFA)‐rich fish oil (FO) around the breeding time improved the utero‐ovarian functions in the goat. Here, we investigated the effect of FO supplementation during the periparturient period on serum n‐3 PUFA, prostaglandin F2α metabolite (PGFM), placental expulsion, uterine involution, resumption of oestrus and neonatal vigour. Rohilkhandi goat in advanced gestation (n = 16) was divided into two equal groups. One group was supplemented with FO containing 26% n‐3 long‐chain PUFA at the rate of 156 mg per kg body weight, while the control group was fed isocaloric palm oil (PO) from ?3 to +3 week of kidding. Dietary FO increased serum concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by 7.3‐ and 6.6‐fold, respectively, after 6 weeks of supplementation. Goats in FO group expelled the foetal membranes 99.1 min earlier (p < .01) than those of PO group. Further, dietary FO significantly decreased the serum PGFM on day 7 post‐partum. However, no difference was found on uterine involution, which was complete by day 20 post‐partum in either group. Resumption of follicular activity by day 5 post‐partum was 87.5% in the FO as compared to 25% in the PO group (p < .05). Similarly, occurrence of behavioural oestrus by day 90 post‐partum was 57.1% in goats of the FO group while none of does was in the PO group (p < .01) expressed oestrus. It was concluded that feeding FO‐rich diet during ?3 to +3 weeks of kidding decreased the PGFM till day 7 post‐partum, hastened the expulsion of foetal membranes and reduced the time from kidding to first post‐partum oestrus in Rohilkhandi does. 相似文献
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