Attractiveness of host banana leaf materials to the banana weevil,Cosmopolites sordidus in Ghana for development of field management strategies     

Samson A Abagale  Christine M Woodcock  Keith Chamberlain  Samuel Osafo‐Acquaah  Helmut van Emden  Michael A Birkett  John A Pickett  Haruna Braimah 《Pest management science》2019,75(2):549-555

  相似文献   

Inhibitory effects of furanone metabolites of a rhizobacterium, Pseudomonas jessenii, on phytopathogenic Aphanomyces cochlioides and Pythium aphanidermatum     

A. Deora†  E. Hatano‡  S. Tahara  Y. Hashidoko 《Plant pathology》2010,59(1):84-99

An antagonistic rhizobacterium, Pseudomonas jessenii EC-S101, isolated from the rhizosphere of spinach, produces two related secondary metabolites, 3-[(1 R )-hydroxyoctyl]-5-methylene-2(5 H )-furanone (4,5-didehydroacaterin) (1) and 3-[(1 R )-hydroxyhexyl]-5-methylene-2(5 H )-furanone (2). This study demonstrated their in vitro inhibitory effects, in particular those of (1), against Aphanomyces cochlioides AC-5 and Pythium aphanidermatum PA-5. The compounds inhibited radial growth and induced morphological abnormalities characterized by hyperbranching and periodic swelling in AC-5 and PA-5 hyphae, respectively. Staining with rhodamine-phalloidin, which binds to plasma-membrane-associated filamentous-actin (F-actin), revealed that tip-specific actin filaments were remodelled into a plaque-like form at an early stage of encounter (up to 24 h) with (1) or (2), whereas at later stages of encounter (48 h), the plaques were eliminated, reflecting the disorganization of actin arrays in the morphologically abnormal AC-5 and PA-5 hyphae. A similar response of actin disorganization was observed in AC-5 and PA-5 hyphae upon treatment with latrunculin B (3), an actin-assembly inhibitor produced by a sea sponge. It is suggested that (1) and (2) caused actin disorganization and their inhibitory activities were comparable to that of (3). Further ultrastructural observations substantiated abnormal functioning and delocalization of F-actin-linked cell organelles.  相似文献   

A case report of Thelazia infection in a 15-month old heifer in Vom, Plateau State, Nigeria     

D A Salifu  E S Haruna  A A Makinde  S T Ajayi 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1990,43(2):197-198

A case of eye infection in a heifer was reported with bilateral blindness, cornea opacity, excessive lachrymation and nasal discharge. Treatment with 6-10 drops of a 10% solution of levamisole resulted in a complete recovery, a total of 127 adult Thelazia rhodesii being recovered from the eyes.  相似文献   

Na?ve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells     

Kimiko HONSHO  Michiko HIROSE  Masanori HATORI  Lubna YASMIN  Haruna IZU  Shogo MATOBA  Sumie TOGAYACHI  Hiroyuki MIYOSHI  Tadashi SANKAI  Atsuo OGURA  Arata HONDA 《The Journal of reproduction and development》2015,61(1):13-19

Quality evaluation of pluripotent stem cells using appropriate animal models needs to be improved for human regenerative medicine. Previously, we demonstrated that although the in vitro neural differentiating capacity of rabbit induced pluripotent stem cells (iPSCs) can be mitigated by improving their baseline level of pluripotency, i.e., by converting them into the so-called “naïve-like” state, the effect after such conversion of rabbit embryonic stem cells (ESCs) remains to be elucidated. Here we found that naïve-like conversion enhanced the differences in innate in vitro differentiation capacity between ESCs and iPSCs. Naïve-like rabbit ESCs exhibited several features indicating pluripotency, including the capacity for teratoma formation. They differentiated into mature oligodendrocytes much more effectively (3.3–7.2 times) than naïve-like iPSCs. This suggests an inherent variation in differentiation potential in vitro among PSC lines. When naïve-like ESCs were injected into preimplantation rabbit embryos, although they contributed efficiently to forming the inner cell mass of blastocysts, no chimeric pups were obtained. Thus, in vitro neural differentiation following naïve-like conversion is a promising option for determining the quality of PSCs without the need to demonstrate chimeric contribution. These results provide an opportunity to evaluate which pluripotent stem cells or treatments are best suited for therapeutic use.  相似文献   

Brazilian savanna re-establishment in a monoculture forest:diversity and environmental relations of native regenerating understory in Pinus caribaea Morelet. stands     

Gastao Viegas de Pinho Ju nior  Andre R Terra Nascimento  Barbara Tahara Valverde  Lucas H Clemente 《林业研究》2015,(3)

In this paper we analyze and compare natural regeneration in shrub-tree community in three areas below Pinus caribaea stands and a natural area (cerrado sensu stricto). We also analyze the influence of...  相似文献   

Relationship between relative water content during reproductive development and winter wheat grain yield   总被引：2，自引：0，他引：2  

Makoto Tahara  Brett F. Carver  Richard C. Johnson  Edward L. Smith 《Euphytica》1990,49(3):255-262

Summary Water is often the most limiting factor to winter wheat (Triticum aestivum L.) production in the southern Great Plains of the U.S.A., yet the lack of reliable screening criteria has precluded direct selection for drought resistance in breeding programs. Previous work showed that leaf relative water content (RWC) was highly heritable when measured under field-drought conditions, but its adoption as a screening tool for yield improvement requires further investigation of the genetic relationship between grain yield and RWC. Plants representing high and low yield potential under drought stress, and a random group of plants, were selected from an F₂ population having the pedigree, TAM W-101/Sturdy. Two sets of entries, each comprised of the two parents and 24 F₂-derived lines, were evaluated under a rainshelter in the F₃ (1986) and F₄ (1987) generations to determine differences in leaf RWC during reproductive development. One set of entries did not receive any water after the jointing stage, and the other set was grown under well-watered conditions. A positive relationship was observed between grain yield and RWC measured during anthesis and mid-grain fill, as the high-yield selections maintained a significantly higher RWC than the low-yield selections. Grain yield and RWC were also positively associated among random selections segregating for both traits. Subsequent adjustment of genotype means for differences in reproductive development at time of sampling underscored the need to consider differences in maturity when RWC is the selection criterion.  相似文献   

In vivo antioxidative activity of propolis evaluated by the interaction with vitamins C and E and the level of lipid hydroperoxides in rats   总被引：4，自引：0，他引：4  

Sun F  Hayami S  Haruna S  Ogiri Y  Tanaka K  Yamada Y  Ikeda K  Yamada H  Sugimoto H  Kawai N  Kojo S 《Journal of agricultural and food chemistry》2000,48(5):1462-1465

In vivo antioxidative activity of propolis was evaluated on the basis of ameliorative effects on the oxidative stress induced by vitamin E deficiency in rats. The control group was fed vitamin E-deficient diet, and the propolis group was fed vitamin E-deficient diet supplemented with 1% of propolis for 4 and 8 weeks. Comparisons were made in tissue concentrations of vitamin C, vitamin E, and lipid hydroperoxides between these groups. No significant difference was observed in tissue vitamin E concentration between these groups after both 4 and 8 weeks. After 4 weeks, the plasma vitamin C concentration of the propolis group was significantly higher than that of the control group. After 8 weeks, the tissue concentrations of vitamin C in the kidney, stomach, small intestine, and large intestine of the propolis group were significantly higher than those of the control group. These results suggest that some components of propolis are absorbed to circulate in the blood and behave as a hydrophilic antioxidant that saves vitamin C. The concentration of lipid hydroperoxides in the large intestine of the propolis group was significantly lower than that of the control group after 8 weeks. These results suggest that propolis exerts its antioxidative effect where it is assumed to accumulate, such as on the kidney, where it is excreted, and on the gastrointestinal tract, where propolis influences these tissues even from the outside of the cell.  相似文献   

Non-pathogenic Fusarium strains protect the seedlings of Lepidium sativum from Pythium ultimum     

H. Ishimoto  Y. Fukushi  S. Tahara 《Soil biology & biochemistry》2004,36(3):409-414

Two root-colonizing Fusarium strains, Ls-F-in-4-1 and Rs-F-in-11, isolated from roots of Brassicaceae plants, induced the resistance in Lepidium sativum seedlings against Pythium ultimum. These strains caused an increase in the content of benzyl isothiocyanate, and of its precursor glucotropaeolin, in the roots of the host plants. The increased isothiocyanate content is one of the factors contributing to the resistance of L. sativum against P. ultimum. To be transformed into the fungitoxic compound benzyl isothiocyanate, glucotropaeolin has to be hydrolyzed by myrosinase, which can be produced either by plants or microorganisms. The Fusarium strain Ls-F-in-4-1 has a myrosinase activity but the strain Rs-F-in-11 has not. These results suggest that both strains are able to trigger the metabolic pathway leading to benzyl isothiocyanate production in the plant. In the case of the myrosinase-negative strain Rs-F-in-11, hydrolyzation into isothiocyanate is only due to the myrosinase activity of the plant, and in the other case, the myrosinase produced by the strain Ls-F-in-11 also would contribute to the production of isothiocyanate. This paper reports a new mode of action of non-pathogenic Fusarium strains in controlling P. ultimum.  相似文献   

Isolation and characterization of staphylococci from external auditory meatus of dogs with or without otitis externa with special reference to Staphylococcus schleiferi subsp. coagulans isolates     

Yamashita K  Shimizu A  Kawano J  Uchida E  Haruna A  Igimi S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(3):263-268

Staphylococci were isolated from the external auditory meatus in 14 (48.3%) of 29 dogs affected with otitis externa (OE dogs) and 28 (68.3%) of 41 dogs without OE (non-OE dogs). Twenty-two OE isolates were identified as belonging to 12 species, and 42 non-OE isolates were identified as belonging to 13 species. The predominant species found in both OE and non-OE isolates were S. intermedius, and S. epidermidis. Thirty-eight (59.4%) of 64 isolates were resistant to one or more of the 17 antimicrobial agents tested. Resistance to PCG and ABPC was most frequent. S. schleiferi subsp. coagulans, a recent etiologic agent of canine OE, was isolated from OE and non-OE dogs. All of the 5 S. schleiferi subsp. coagulans isolates showed typical characteristics. No clear difference in the extracellular enzyme or toxin profiles, nor in the PFGE patterns, was demonstrated between the OE and non-OE isolates of S. schleiferi subsp. coagulans. A new PCR primer set specific for 16S rDNA was designed to identify strains of S. schleiferi subsp. coagulans. The amplified fragment was detected in all of the 5 isolates as well as the type strain GA 211 (=JCM 7470) and a reference strain GA 11, but was not detected in any strains of the related species, S. aureus, S. intermedius and S. hyicus. The PCR may allow a simple, rapid and precise identification of S. schleiferi subsp. coagulans, in addition to the standard tube test for free coagulase.  相似文献   

Antagonistic rhizoplane bacteria induce diverse morphological alterations in Peronosporomycete hyphae during <Emphasis Type="Italic">in vitro</Emphasis> interaction     

Abhinandan?Deora  Yasuyuki?HashidokoEmail author  Md.?Tofazzal?Islam  Satoshi?Tahara 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(4):311-322

A total of 150 bacteria were isolated from rhizoplanes of the host and non-host plants of a phytopathogenic Peronosporomycete Aphanomyces cochlioides. Upon screening, 5% of the isolates were evaluated as antagonists as they inhibited radial growth of A. cochlioides AC-5 hyphae in a dual culture assay. In addition, those antagonistic bacteria also induced characteristic morphological alterations in the A. cochlioides AC-5 hyphae that grew towards bacterial colonies. Hyphal morphological alterations observed in AC-5 and other tested strains of Peronosporomycetes included excessive branching, curly growth, unusually longer and pointed tip formation and swelling; all of these were comparable to the alterations induced by known antimicrobial compounds. Among the antagonistic bacteria, Pseudomonas sp. strain EC-S101 induced a unique branching pattern (tree-like) in AC-5 hyphae by continuous apical bifurcation of successive hyphae, where increases in number of branches and hyphal area were linearly correlated with time up to 10 h. Our observations suggested that the pathogen might have lost its ability of normal branch production; however maintained the capability of self-branching. Soluble extracts from the culture fluids of Pseudomonas sp. strain EC-S101 and Stenotrophomonas maltophilia EC-S105 induced similar excessive branching and curly growth in A. cochlioides hyphae as the respective bacterium. These results revealed that bacterial metabolites appeared to be responsible for induction of morphological alterations. Interestingly, the antagonistic bacteria that induced hyphal morphological alterations, also efficiently suppressed in vivo damping-off disease caused by AC-5. We suggest that antagonistic rhizoplane bacteria have the capability to induce diverse morphological alterations in Peronosporomycetes hyphae during in vitro interactions. Hyphal morphological alterations associated with growth inhibition and the induction of characteristic morphological changes indicate antagonistic activity against the Peronosporomycete.  相似文献