Characterization of ovine strains of Mycobacterium paratuberculosis by restriction endonuclease analysis and DNA hybridization.     

G W De Lisle  D M Collins  H F Huchzermeyer 《The Onderstepoort journal of veterinary research》1992,59(2):163-165

Restriction endonuclease analysis and DNA hybridization revealed five ovine strains of Mycobacterium paratuberculosis from South Africa had identical DNA patterns to an ovine strain from Canada. Genetically this strain type has features in common with the two major groups of M. paratuberculosis.  相似文献   

Diarrhea associated with Clostridium perfringens type A enterotoxin in neonatal pigs   总被引：4，自引：0，他引：4  

J E Collins  M E Bergeland  D Bouley  A L Ducommun  D H Francis  P Yeske 《Journal of veterinary diagnostic investigation》1989,1(4):351-353

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Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls.   总被引：3，自引：0，他引：3       下载免费PDF全文

M D Bierk  S A Dee  K D Rossow  S Otake  J E Collins    T W Molitor 《Canadian journal of veterinary research》2001,65(4):261-266

The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals.  相似文献   

Effects of stable dust on farm animals--a review     

M Collins  B Algers 《Veterinary research communications》1986,10(6):415-428

The relationship between air quality--its dust content--and respiratory diseases of animals in the food industry and otherwise is examined. The correlation between dust content and incidence of disease in farm animals is also discussed. Lesions caused either directly or indirectly by dust-transported microorganisms are reviewed. Effects of dust on animal performance are included. This review is an attempt to compile available information on the natural occurrence of stable dust and the possibilities for its measurement and control.  相似文献   

OBITUARIES: Dr. Arnley Quackenbush          下载免费PDF全文

John Henry 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(11):852

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Multiresidue assay for benzimidazole anthelmintics by liquid chromatography and confirmation by gas chromatography/selected-ion monitoring electron impact mass spectrometry     

R T Wilson  J M Groneck  A C Henry  L D Rowe 《Journal of the Association of Official Analytical Chemists》1991,74(1):56-67

A multiresidue method utilizing all-disposable labware has been developed for 8 benzimidazole anthelmintics from ovine, bovine, and swine muscle and liver tissues. After an initial extraction with ethyl acetate and subsequent evaporation, a 3-component extraction using hexane, ethanol, and 0.2N HCl was used for final cleanup. Clean extracts were produced for separation and determination by reverse-phase liquid chromatography at 298 nm, using methanol and aqueous buffer as mobile phase. A synthesized internal standard, 2-(n-butylmercapto)benzimidazole, was used for quantitation of all drugs. Results are included along with statistical information verifying the performance of the method. Spiked control tissues and incurred drug tissues were used for an intralaboratory study with a concentration range of 50-1470 ppb. A series of standard curves at 0, 50, 100, and 200 ppb were analyzed. Overall recovery at the 100 ppb level averaged 92% (CV 8%) in liver tissues, across all 3 species and 88% (CV 5%) in muscle tissues across all 3 species. Results were confirmed by gas chromatography/mass spectrometry with acid hydrolysis of the remaining extract in 2N HCl followed by re-extraction of the amine and derivatization to the tert-butyldimethylsilyl derivative. The anthelmintics were identified by gas chromatography/selected ion monitoring electron-impact mass spectrometry. Ion ratio measurements were taken and compared to standard material. CVs averaged 10% or less for all drugs tested.  相似文献   

Estimation of the relative bioavailability of copper sources in chicks fed on conventional dietary amounts     

M. A. Zanetti  P. R. Henry  C. B. Ammerman  R. D. Miles 《British poultry science》1991,32(3):583-588

1. Tissue accumulation of Cu from dietary additions of 0, 5, 10, or 20 mg/kg Cu as reagent grade Cu acetate and feed grade Cu carbonate was determined in day‐old chicks fed on conventional maize‐soyabean meal starter diets (5.41 mg/kg Cu as‐fed basis) for 3 weeks.

2. Average daily food intake, daily weight gain and food conversion were similar among treatments.

3. There were linear increases in plasma and liver Cu concentrations (P< 0.01) as dietary Cu increased.

4. Bioavailability of Cu as carbonate was 0.66 that of Cu in the acetate based on the multiple regression slope ratio of liver Cu concentration on added dietary Cu. Although responses for the two Cu sources did not differ significantly, the relative bioavailability of the Cu carbonate was similar (0.66 vs 0.68) to that obtained in an earlier study (Ledoux et al., 1991) with greater dietary Cu contents (150, 300 and 450 mg/kg) in which the slopes of the equations representing the two sources differed (P<0.05).  相似文献   

Typing of Mycobacterium bovis isolates from cattle and other animals in the same locality     

Collins DM  Gabric DM  de Lisle GW 《New Zealand veterinary journal》1988,36(1):45-46

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New Zealand caprine herpes virus: comparison with an Australian isolate and with bovine herpes virus type 1 by restriction endonuclease analysis     

Tisdall DJ  Bentley CB  Collins DM  Horner GW 《New Zealand veterinary journal》1984,32(6):99-100

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Studies on Sarcocystis species. IV: A species infecting dogs and goats; development in goats.     

G H Collins  W A Charleston 《New Zealand veterinary journal》1979,27(12):260-262

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