Haematological and biochemical investigations of diseased koalas (Phascolarctos cinereus)     

PJ CANFIELD  ME O'NEILL†  EF SMITH† 《Australian veterinary journal》1989,66(9):269-272

Haematological and biochemical investigations were performed on 14 koalas with uncomplicated cystitis, 8 with complicated cystitis, 8 with conjunctivitis, 8 with lymphosarcoma, and 14 with miscellaneous diseases. Changes were limited and inconsistent in individual koalas with uncomplicated cystitis and conjunctivitis. In contrast, individual koalas with complicated cystitis were more likely to have anaemia, leukocytosis due to neutrophilia, hypoproteinaemia due to hypoalbuminaemia, and azotaemia due to elevated urea concentration. Although these changes were non-specific they did allow assessment of prognosis for survival and response to treatment. Koalas with lymphosarcoma were invariably anaemic, leukaemic, azotaemic and hypoalbuminaemic. Elevated enzymes (aspartate transaminase [AST]. lactate dehydrogenase [LD] and gamma glutamyl transferase [GGT]) were more common in koalas with lymphosarcoma. Koalas affected by miscellaneous conditions showed variable changes but once again anaemia, leukocytosis, azotaemia, elevated AST and LD, and hypoalbuminaemia were not uncommon. On the basis of these findings a minimal profile is suggested for the investigation of sick koalas and would include haematocrit, total and differential leukocyte counts, urea, total protein and albumin concentrations and AST, GGT and LD activities.  相似文献   

The Influence of a Particle Separation Technology on the Generation of Airborne Particles from Different Roughages and Bedding Materials Used for Horses     

Felix Garlipp  MSc  Engel F. Hessel  Matthieu van den HurkMark F. Timmerman  Dr.  Herman F.A. Van den Weghe 《Journal of Equine Veterinary Science》2010

Four different types of bedding materials (wheat straw, wood shavings, hemp shives, flax shives) and two roughages (hay, haylage) were treated using an air-driven particle separation technology. The airborne particle and mold generation of both treated and untreated samples were then analyzed under standardized laboratory conditions. In addition, samples of all the treated materials were stored for 8 weeks either in a pressed or incoherent form and then analyzed again for their ability to generate airborne particles. The airborne particle concentrations were detected online with the gravimetrically measuring analyzer tapered element oscillating microbalance (TEOM) 1400a that was equipped successively with different inlets to measure the particle fractions PM₂₀, PM₁₀, PM_2.5, and PM_1.0 (PM = Particulate matter). The particle separation resulted in a reduction in the airborne particle (PM₂₀) generation in all materials: hay 49.16 to 22.79 mg/m³ (53.6%), haylage 28.57 to 25.04 mg/m³ (12.3%), wood shavings 141.68 to 15.04 mg/m³ (89.4%), wheat straw 143.08 to 22.97 mg/m³ (83.9%), flax 135.11 to 53.31 mg/m³ (60.5%), and hemp 63.67 to 17.64 mg/m³ (72.3%). The 8-week storage of the treated materials as compressed materials led to a renewed significant increase in the airborne particle (PM₁₀) concentration in the haylage (+29.9%), wheat straw (+104.0%), wood shavings (+40.4%), and hemp shives (+30.7%). Storage of the incoherent materials caused a significant increase in these particles only in the wheat straw (+44.2%). The separation treatment reduced the mold production by 92.4% in the wood shavings, 88.0% in the wheat straw, and 85.8% in the hay.  相似文献   

Evaluation of Factors Influencing the Generation of Ammonia in Different Bedding Materials Used for Horse Keeping     

K. Fleming MSc  E.F. Hessel PD Dr.  H.F.A. Van den Weghe Prof. Dr. Ir. 《Journal of Equine Veterinary Science》2008,28(4):223-231

Bedding material is an important factor in determining stable air quality in terms of ammonia formation. The objective of this study was to analyze different bedding materials used for horse stables under standardized conditions, to determine which material is best suited for improving the climate of a stable. The particular concern was a reduction in gaseous ammonia concentrations. Therefore, the following materials were examined: wheat straw, wood shavings, hemp shives, linen shives, wheat straw pellets, and paper cuttings. Twelve containers were constructed in an environmentally controlled room, and the same material was placed into two containers, with the amount of material used being determined by its carbon content. A defined ratio of horse manure/urine mixture was added daily to each container over a period of 14 days. The concentrations of gaseous ammonia, carbon dioxide, nitrous oxide, and water vapor were measured continuously above the bedding within the containers. Means of gaseous ammonia were found to be 178.0 mg/m³ for wheat straw, 155.2 mg/m³ for wood shavings, 144.6 mg/m³ for hemp, 133.7 mg/m³ for linen, 60.3 mg/m³ for straw pellets, and 162.6 mg/m³ for paper cuttings. In conclusion, the results of this study have shown that straw pellets are suitable for horse stables, not only to improve air quality but also, first and foremost, in relation to ammonia binding and ammonia transformation within the bedding material, respectively. However, straw pellets may also have disadvantages. The high substrate temperatures that were measured in straw pellets could favor the growth of pathologic germs that can adversely affect animals' health.  相似文献   

Generation of Airborne Particles from Horse Feeds Depending on Type and Processing     

Engel F. Hessel  Felix GarlippHerman F.A. Van den Weghe  Prof Dr Ir 《Journal of Equine Veterinary Science》2009

Among other factors (eg, bedding material, roughage), feed has an important effect on the airborne particle concentration in the stable air, in particular directly in the breathing zone of the horse during feeding. Therefore, this study was designed to analyze and compare the generation of different airborne particle fractions of diverse feed types and feed processing techniques under standardized laboratory conditions. Oats and barley as well as two commercially formulated ready-prepared pellets and two different commercially formulated ready-prepared mixes were analyzed. Oats were subjected to four different forms of processing (whole uncleaned, whole cleaned, rolled uncleaned, rolled cleaned), barley was subjected to steam flaking in addition to the same forms of processing as oats. In total, 13 feeds were analyzed. Airborne particle concentrations were detected online with a gravimetrically measuring analyzer that was equipped successively with different inlets to measure the particle fractions PM 1, PM 2.5, PM 10, and PM 20. The measurements were carried out in a closed chamber (1 × 1 × 1.5 m) including a bowl (diameter = 0.5 m), which simulated the trough. The sample inlet was situated at a height of 1.0 m above the bowl. Rolling oats and barley led to a significantly increased generation of the PM 20 fraction and a significantly decreased generation of the PM 2.5 fraction. The mechanical cleaning of oat and barley brings a clear decrease in dust generation from straights whether they are left whole or are rolled after cleaning. A considerable reduction in dust generation can be achieved if steam-flaked barley or commercially prepared feeds are used.  相似文献   

Chlamydia infections and heart disease linked through antigenic mimicry     

Bachmaier K  Neu N  de la Maza LM  Pal S  Hessel A  Penninger JM 《Science (New York, N.Y.)》1999,283(5406):1335-1339

Chlamydia infections are epidemiologically linked to human heart disease. A peptide from the murine heart muscle-specific alpha myosin heavy chain that has sequence homology to the 60-kilodalton cysteine-rich outer membrane proteins of Chlamydia pneumoniae, C. psittaci, and C. trachomatis was shown to induce autoimmune inflammatory heart disease in mice. Injection of the homologous Chlamydia peptides into mice also induced perivascular inflammation, fibrotic changes, and blood vessel occlusion in the heart, as well as triggering T and B cell reactivity to the homologous endogenous heart muscle-specific peptide. Chlamydia DNA functioned as an adjuvant in the triggering of peptide-induced inflammatory heart disease. Infection with C. trachomatis led to the production of autoantibodies to heart muscle-specific epitopes. Thus, Chlamydia-mediated heart disease is induced by antigenic mimicry of a heart muscle-specific protein.  相似文献   

Evaluation of Potential Thrombin Inhibitors from the White Mangrove (Laguncularia racemosa (L.) C.F. Gaertn.)     

Caroline Fabri Bittencourt Rodrigues  Henrique Hessel Gaeta  Mariana Novo Belchor  Marcelo José Pena Ferreira  Marcus Vinícius Terashima Pinho  Daniela de Oliveira Toyama  Marcos Hikari Toyama 《Marine drugs》2015,13(7):4505-4519

The aim of this work was to verify the effects of methanol (MeOH) and hydroalcoholic (HA) extracts and their respective partition phases obtained from white mangrove (Laguncularia racemosa (L.) C.F. Gaertn.) leaves on human thrombin activity. Among the extracts and phases tested, only the ethyl acetate and butanolic partitions significantly inhibited human thrombin activity and the coagulation of plasma in the presence of this enzyme. Chromatographic analyses of the thrombin samples incubated with these phases revealed that different compounds were able to interact with thrombin. The butanolic phase of the MeOH extract had the most potent inhibitory effects, reducing enzymatic activity and thrombin-induced plasma coagulation. Two glycosylated flavonoids in this partition were identified as the most potent inhibitors of human thrombin activity, namely quercetin-3-O-arabinoside (QAra) and quercetin-3-O-rhamnoside (Qn). Chromatographic analyses of thrombin samples incubated with these flavonoids demonstrated the chemical modification of this enzyme, suggesting that the MeOH extract contained other compounds that both induced structural changes in thrombin and diminished its activity. In this article, we show that despite the near absence of the medical use of mangrove compounds, this plant contains natural compounds with potential therapeutic applications.  相似文献   

Socializing piglets before weaning: effects on behavior of lactating sows, pre- and postweaning behavior, and performance of piglets     

Hessel EF  Reiners K  Van den Weghe HF 《Journal of animal science》2006,84(10):2847-2855

This study evaluated how socializing piglets before weaning affects behavior of lactating sows and the pre- and postweaning behavior and performance of piglets. Two farrowing rooms, each with 6 pens, and 1 nursery with 4 pens were used. In total, data were obtained from 24 sows and their litters. In each farrowing room, the solid barriers between 3 farrowing pens were removed on d 12 after farrowing, and the sows remained confined in their crates (experimental group). In the other 3 farrowing pens of each farrowing room, sows and their litters were kept under conventional conditions until weaning (control group). All piglets were weaned 28 d after birth. After weaning, piglets from each group remained together in 1 pen of the nursery. The behavior of sows (lying, standing, sitting, nursing) and piglets (lying, active, suckling) in the farrowing rooms was observed for 24 h before and for 48 h after removal of the barriers between the pens. In addition, behavior (active, lying, feeding, agonistic behavior) of piglets was observed in the nursery during the initial 48-h period after weaning. Each piglet was weighed on d 5, 12, and 28 after birth and thereafter weekly until the fifth week of rearing. In the farrowing room, mixing of litters did not influence behavior of piglets and sows. Preweaning weight gain of the piglets did not differ (P = 0.60) between the treatments. In the initial 48 h after weaning, less agonistic behavior (P < 0.001) was observed in piglets belonging to the experimental group. During 5 wk of rearing, piglets in the experimental group gained more weight compared with the control group (P = 0.05). The advantage shown by the experimental group became especially conspicuous in the first week after weaning (P = 0.05). By socializing unfamiliar piglets before weaning, stress due to mixing could at least be distanced in time from the other burdens of weaning, thereby improving performance.  相似文献   

Influence of increased feeding frequency on behavior and integument lesions in growing-finishing restricted-fed pigs     

Hessel EF  Wülbers-Mindermann M  Berg C  Van den Weghe HF  Algers B 《Journal of animal science》2006,84(6):1526-1534

This study evaluated how feeding frequency affects behavior and the occurrence of skin lesions in growing-finishing pigs. One hundred eighty pigs (27 to 112 kg of BW) were reared in one environmentally controlled room (20 pens; 9 pigs/pen). Pigs in 10 pens were fed 3 times daily (reference group), whereas the others were fed 9 times daily (experimental group). Both groups received the same total amount of liquid feed. Rations were adjusted to the mean pen weights. Behavioral observations (scan sampling, as well as continuous focal pig observations) were made in wk 4, 10, and 14 of the growing-finishing period. After each observation, skin lesions were assessed individually for each pig. Pigs fed 9 times daily tended to lie laterally for less time (P = 0.083) and tended to be active (P = 0.054) during the day, especially in growing-finishing wk 4 (P = 0.007). With continuously observed focal pigs, no differences in time allocations for feeding were found between groups. During feeding in growing-finishing wk 4, focal pigs belonging to the experimental group displayed more aggressive actions (P = 0.019), tended to perform aggressive actions for a longer time (P = 0.076), and tended to be belly-nosed for a longer time (P = 0.083) compared with the reference group. In addition, in growing-finishing wk 14, pigs in the experimental group had greater scores for skin lesions (head, P = 0.001; belly, P < 0.001; caudal part, P < 0.001) and tended to be belly-nosed for a longer time (P = 0.084). In the case of pigs restricted-fed liquid feed, a greater frequency of feeding per day appears to be a condition that results in greater competitive feeding than with a lower feeding frequency.  相似文献   

Evaluation of Macroalgae Sulfated Polysaccharides on the Leishmania (L.) amazonensis Promastigote     

Camila Lehnhardt Pires  Selma Dzimidas Rodrigues  Daniel Bristot  Henrique Hessel Gaeta  Daniela de Oliveira Toyama  Wladimir Ronald Lobo Farias  Marcos Hikari Toyama 《Marine drugs》2013,11(3):934-943

The sulfated polysaccharides from Solieria filiformis (Sf), Botryocladia occidentalis (Bo), Caulerpa racemosa (Cr) and Gracilaria caudata (Gc) were extracted and extensively purified. These compounds were then subjected to in vitro assays to evaluate the inhibition of these polysaccharides on the growth of Leishmania (L.) amazonensis promastigotes. Under the same assay conditions, only three of the four sulfated polysaccharides were active against L. amazonensis, and the polysaccharide purified from Cr was the most potent (EC₅₀ value: 34.5 μg/mL). The polysaccharides derived from Bo and Sf demonstrated moderate anti-leishmanial activity (EC₅₀ values of 63.7 μg/mL and 137.4 μg/mL). In addition, we also performed in vitro cytotoxic assays toward peritoneal macrophages and J774 macrophages. For the in vitro cytotoxicity assay employing J774 cells, all of the sulfated polysaccharides decreased cell survival, with CC₅₀ values of 27.3 μg/mL, 49.3 μg/mL, 73.2 μg/mL, and 99.8 μg/mL for Bo, Cr, Gc, and Sf, respectively. However, none of the sulfated polysaccharides reduced the cell growth rate of the peritoneal macrophages. These results suggest that macroalgae contain compounds with various chemical properties that can control specific pathogens. According to our results, the assayed sulfated polysaccharides were able to modulate the growth rate and cell survival of Leishmania (L.) amazonensis promastigotes in in vitro assays, and these effects involved the interaction of the sulfated polysaccharides on the cell membrane of the parasites.  相似文献   

Influence of paddy rice terraces on soil erosion of a small watershed in a hilly area of Northern Vietnam     

Van Trinh Mai  Herman van Keulen  Rudi Hessel  Coen Ritsema  Reimund Roetter  Thai Phien 《Paddy and Water Environment》2013,11(1-4):285-298

Soil erosion is the main cause of soil degradation in northern Vietnam. In this study, soil erosion was measured in 2 m² field plots, a 19.1-ha sub-watershed, and a 248.9-ha main watershed in Tam Quan commune, Tam Duong district, northern Vietnam during 2 years, i.e., 2004–2005. The main watershed includes lowland paddy fields, and is representative for watersheds in the northern Vietnamese landscape. Soil erosion was measured for eight events, at all the three scales to increase our understanding of erosional processes and to assess the effects of paddy fields within the main watershed. The results show that total discharge and sediment yield in both sub-watershed and main watershed were much lower than those in the field plots. Total discharge per unit area in the main watershed was higher than in the sub-watershed, because during the growing season, the paddies are filled with water and any rainfall on them therefore becomes runoff. Sediment yield in the main watershed fluctuated, depending on the soil erosion contribution from many sub-watersheds. Annual rainfalls in 2004 and 2005 were 1,172 and 1,560 mm, respectively, resulting in corresponding total discharges of 54 and 332 mm and total soil losses of 163 and 1,722 kg ha^?1 year^?1. High runoff volumes occurred in July, August, and September, but April, June, the last 10 days of September and October, were the susceptible periods for soil erosion in the study area because of low plant cover and many agricultural activities during these periods.  相似文献