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Chiho KANEKO Yasuyuki KANEKO Putu Eka SUDARYATMA Hirohisa MEKATA Yumi KIRINO Ryoji YAMAGUCHI Tamaki OKABAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):680
We isolated two pseudorabies virus (PRV) isolates (designated OT-1 and OT-2) from two hunting dogs exhibiting neurological manifestations after eating the flesh of wild boar hunted in Oita prefecture, Kyushu Island, Japan. The isolates corresponded to a previously reported PRV (MY-1 strain) isolated from a hunting dog in neighboring Miyazaki prefecture, and it clustered into genotype II based on the glycoprotein C sequence. Our results suggest that this common PRV strain may have been maintained in wild boars on Kyushu Island even though domestic pigs in this area have attained an Aujeszky’s disease-free status. 相似文献
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Matsubara H Yoshimoto S Saito H Jianglin Y Tanaka Y Noda S 《Science (New York, N.Y.)》2008,319(5862):445-447
Shorter-wavelength surface-emitting laser sources are important for a variety of fields, including photonics, information processing, and biology. We report on the creation of a current-driven blue-violet photonic-crystal surface-emitting laser. We have developed a fabrication method, named "air holes retained over growth," in order to construct a two-dimensional gallium nitride (GaN)/air photonic-crystal structure. The resulting periodic structure has a photonic-crystal band-edge effect sufficient for the successful operation of a current-injection surface-emitting laser. This represents an important step in the development of laser sources that could be focused to a size much less than the wavelength and be integrated two-dimensionally at such short wavelengths. 相似文献
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Tomoaki ONODA Ryuta YAMAMOTO Kyohei SAWAMURA Harutaka MURASE Yasuo NAMBO Yoshinobu INOUE Akira MATSUI Takeshi MIYAKE Nobuhiro HIRAI 《Journal of Equine Science》2013,24(4):63-69
Percentile growth curves are often used as a clinical indicator to evaluate variations of
children’s growth status. In this study, we propose empirical percentile growth curves
using Z-scores adapted for Japanese Thoroughbred horses, with considerations of the
seasonal compensatory growth that is a typical characteristic of seasonal breeding
animals. We previously developed new growth curve equations for Japanese Thoroughbreds
adjusting for compensatory growth. Individual horses and residual effects were included as
random effects in the growth curve equation model and their variance components were
estimated. Based on the Z-scores of the estimated variance components, empirical
percentile growth curves were constructed. A total of 5,594 and 5,680 body weight and age
measurements of male and female Thoroughbreds, respectively, and 3,770 withers height and
age measurements were used in the analyses. The developed empirical percentile growth
curves using Z-scores are computationally feasible and useful for monitoring individual
growth parameters of body weight and withers height of young Thoroughbred horses,
especially during compensatory growth periods. 相似文献
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Fulka H Martinkova S Kyogoku H Langerova A Fulka J 《The Journal of reproduction and development》2012,58(3):371-376
Compared with advanced developmental stage embryos and somatic cells, fully grown mammalian oocytes contain specific nucleolus-like structures (NPB - nucleolus precursor bodies). It is commonly accepted that they serve as a store of material(s) from which typical nucleoli are gradually formed. Whilst nucleoli from somatic cells can be collected relatively easily for further biochemical analyses, a sufficient number of oocyte nucleoli is very difficult to obtain. We have found that isolated oocytes nucleoli fuse very efficiently when contact is established between them. Thus, well visible giant nucleoli can be obtained, relatively easily handled and then used for further biochemical analyses. With the use of colloidal gold staining, we estimated that a single fully grown mouse oocyte nucleolus contains approximately 1.6 ng of protein. We do believe that this approach will accelerate further research aiming at analyzing the composition of oocyte nucleoli in more detail. 相似文献
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Mekata H Konnai S Simuunza M Chembensofu M Kano R Witola WH Tembo ME Chitambo H Inoue N Onuma M Ohashi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(9):923-928
The prevalence of trypanosome infections in tsetse flies, Glossina pallidipes, collected from Chiawa and Chakwenga in Zambia with endemic trypanosomosis was assessed by polymerase chain reaction (PCR). Out of the 550 G. pallidipes, 58 (10.5%) flies were found to harbor trypanosome DNA. Infection rates of tsetse with Trypanosoma vivax universal, Trypanosoma congolense savannah, T. congolense forest and T. congolense kilifi were 4.2% (23/550), 4.7% (26/550), 1.1% (6/550) and 1.6% (9/550), respectively. To determine the mammalian hosts of T. congolense and T. vivax infections from the tsetse flies, mammalian mitochondrion DNA of blood meal in these flies were analyzed by PCR and subsequent gene sequence analysis of the amplicons. Sequence analysis showed the presence of cytochrome b gene (cyt b) of 7 different mammalian species such as human, elephant, buffalo, goat, warthog, greater kudu and cattle. Goats which were main livestock in these areas were further examined to know the extent of its contribution in spreading the infection. We examined the prevalence of trypanosome infections in the domestic goat population in 6 settlements in Chiawa alone. Of the 86 goats sampled, 4 (4.6%), 5 (5.8%), 4 (4.6%) and 4 (4.6%) were positive for T. vivax universal, T. congolense savannah, forest and kilifi, respectively. These findings showed that the host-source of trypanosome infections in vector fly give a vital information about spread of infection. The result of this study will certainly contribute in elucidating more the epidemiology of trypanosomosis. 相似文献
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Hafez E Takahashi T Ogawa H Sato M Nakai T Takasu C Uehara H Izumi K 《Journal of toxicologic pathology》2011,24(4):187-193
Diabetes mellitus (DM) and obesity are believed to be risk factors for colorectal cancer
in humans. In experiment 1, male nondiabetic Long-Evans Tokushima Otsuka (LETO) rats and
Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model animal of type 2 DM, were
whole-body X-irradiated (4 Gy) at 6 and 8 weeks of age and euthanized at 78 weeks of age
(n=15, respectively). The incidences of small intestine adenocarcinoma in LETO and OLETF
rats were 0% and 30%, respectively. In experiment 2, male LETO and OLETF rats (n=24,
respectively) were given s.c. injections of 15 mg/kg azoxymethane (AOM) once weekly for 3
weeks and euthanized at 36 weeks of age. The incidences of Zymbal gland tumors in LETO and
OLETF rats were 0% and 67%, respectively (P<0.001), whereas those of
small intestine adenocarcinoma were 0% and 43% (P<0.001) and those of
cecum/colon adenocarcinoma were 46% and 79% (P<0.05), respectively.
Fatty change of hepatocytes was common in OLETF rats (63%) but not in LETO rats. Serum
triglyceride and free fatty acid levels in OLETF rats were significantly higher than in
LETO rats at sacrifice, whereas serum insulin levels in OLETF rats were very diverse.
These data suggest that hyperlipidemia plays a significant role in high susceptibility to
lower intestinal tract carcinogenesis in OLETF rats; this strain is susceptible to
AOM-induced Zymbal gland carcinogenesis. 相似文献
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Akamatsu H Saitoh Y Serizawa M Miyake K Ohba Y Nakashima K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(10):1091-1093
The present study examined the Serum 3-methylhistidine concentrations and energy-associated variables of 5 healthy Holstein cows and 5 Holstein cows with ketosis. The serum total cholesterol and apolipoprotein B-100 concentrations and lecithin-cholesterol acyltransferase (LCAT) activity of the ketotic cows were lower than those of the healthy cows 14 days before parturition. The serum non-esterified fatty acid (NEFA) concentration on the day of parturition and 3-methylhistidine concentration 14 days after parturition were higher in the ketotic cows. The serum 3-methylhistidine concentration 14 days after parturition was negatively correlated with the serum LCAT activity 14 days before parturition and was positively correlated with the serum NEFA concentration on the day of parturition. Insufficiency of cholesterol metabolism and acceleration of body fat degradation occur before parturition in cows with ketosis, and these characteristics are correlated with acceleration of protein degradation after parturition. 相似文献