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Hiromitsu Kisanuki Hisae Oguro Arisa Nakai Suzuki Setsuko Naoyuki Nishimura Nobuhiro Tomaru 《Journal of Forest Research》2008,13(2):143-146
We studied seed bank formation of the threatened star magnolia, Magnolia stellata, to examine the early stage of regeneration. Forty-five seedling plots (2 × 2 m), each including a soil-sampling quadrat
(40 × 40 cm), were established randomly under or around the crowns of mature M. stellata trees. Seeds of M. stellata were collected from each quadrat to a depth of 5 cm. Only four seeds of M. stellata were found (0.56 seeds/m2) and all were located under mature crowns. Current-year seedlings were abundant in water channels, on moss, or under mature
crowns, suggesting that the seeds may require wet soil conditions for germination. Magnolia stellata seeds show considerable germination below the crowns of mature trees in the year following masting, while some seeds remain
dormant in the soil. Considering the soil seed bank and the current-year seedling bank of M. stellata, a frequent supply of seed is essential for the regeneration of this species. Thus, it is important to maintain mature trees
in addition to promoting seed production. 相似文献
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Sang-gun ROH Yeon-hee HONG Daisuke HISHIKAWA Hiroaki TSUZUKI Hisae MIYAHARA Yukihiko NISHIMURA Chizu GOTO Yasuki SUZUKI Ki-choon CHOI Hong-gu LEE Shinichi SASAKI 《Animal Science Journal》2005,76(4):381-386
Ghrelin and growth hormone secretagogues receptor (GHS‐R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS‐R in adipocytes, the effect of the GHS‐R antagonist, [D‐Lys‐3]‐GHRP‐6 (His‐D‐Trp‐D‐Lys‐Trp‐D‐Phe‐Lys‐NH2), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D‐Lys‐3]‐GHRP‐6 (10?7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)‐γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D‐Lys‐3]‐GHRP‐6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol‐3‐phosphate dehydrogenase and upregulation of PPAR‐γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D‐Lys‐3]‐GHRP‐6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS‐R in adipogenesis can be blocked by treatment with [D‐Lys‐3]‐GHRP‐6. 相似文献
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Dickeya dadantii (syn. Erwinia chrysanthemi) is a causal agent of soft-rot diseases on many crops. Here, we characterized a gene belonging to the major facilitator superfamily
(MFS), which is involved in the symport, antiport, or uniport of various substrates, and the survival and virulence of many
Gram-negative bacterial animal pathogens, for the possible involvement in the plant pathogenicity of D. dadantii. A marker-exchange mutant of this gene (mfsX) was constructed that had decreased maceration ability in Chinese cabbage, potato, and chicory. Observation with electron
microscopy showed greatly reduced numbers of flagella per cell. This mutant had a significant reduction in swimming and swarming
motility and a severe reduction in formation of biofilm. Because these phenotypes have been shown to be involved in plant
pathogenicity of D. dadantii, mfsX seems to play an important role in pathogenesis of D. dadantii 3937 by its involvement in the expression of these pathogenicity-related phenotypes. 相似文献
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Luqman Qurata Aini Hisae Hirata Shinji Tsuyumu 《Journal of General Plant Pathology》2010,76(2):132-142
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Kuniyoshi Yasukawa Shiori Saito Takuya Kubo Yuya Shibasaki Kayo Yamaoka Hisae Hachimura Tomoko Kuyama Akiteru Amimoto Tsuyoshi Kumata Yuko Kitahara Masahiko Takenaka Hitoshi Matsumura Takehiro Uno Tomiya Uchino Kazutaka Takehara Kouji Nishida Michiyo Kadoya Masafumi Sato Kaoru Kato Kanako Matsumoto Satoshi Saito Tetsuya Shimoda 《Veterinary dermatology》2010,21(1):42-49
The purpose of this study was to investigate the minimum effective dose of recombinant canine interferon-γ (rCaIFN-γ) for the treatment of dogs with atopic dermatitis (AD). Thirty-four dogs with AD from 17 animal hospitals in Japan were administered half or one-fifth of the approved rCaIFN-γ dose of 10 000 units/kg, three times a week for 4 weeks, followed by once weekly for an additional 4 weeks. Pruritus, excoriation, erythema and alopecia were evaluated and scored by the investigators on weeks 2, 4, 6, 8 and 12. The efficacy rate (number of excellent cases + number of good cases/total number of cases) at week 8 in the 2000 units/kg group was 36.4% for pruritus, 36.4% for excoriation, 45.5% for erythema and 36.4% for alopecia. In contrast, in the 5000 units/kg group, the efficacy rate was 64.3% for pruritus, 57.1% for excoriation, 78.6% for erythema and 78.6% for alopecia. The efficacy rate of the 5000 units/kg group was high for all signs evaluated and comparable to that of the 10 000 units/kg group reported in a previous study. The results of this study showed that 2000 units/kg of rCaIFN-γ is less effective than 5000 units/kg to treat dogs with AD, and the efficacy of the 5000 units/kg dose is comparable to that of 10 000 units/kg at week 8. 相似文献