C-reactive protein concentrations in canine acute pancreatitis   总被引：1，自引：0，他引：1  

Jennifer L. Holm  DVM    Elizabeth A. Rozanski  DVM  DACVECC  DACVIM    Lisa M. Freeman  DVM  PhD  DACVN    Cynthia R. L. Webster  DVM  DACVIM 《Journal of Veterinary Emergency and Critical Care》2004,14(3):183-186

Objective: To determine if C‐reactive protein (CRP) concentration is elevated in spontaneously occurring canine acute pancreatitis (AP), and to measure changes in CRP during the course of hospitalization. Design: Prospective study. Setting: Tufts University School of Veterinary Medicine Foster Hospital for Small Animals. Animals: Sixteen client‐owned dogs with AP and 16 healthy controls. Interventions: Blood samples were obtained from the AP group on the day of diagnosis (Day 1), and on Days 3 and 5, unless the dog died or was discharged from the hospital. Blood was obtained from the control dogs once. Measurements and main results: Serum CRP was measured using a commercial immunoassay for each dog with AP and for healthy controls. Day 1 CRP concentrations were significantly higher in the AP group (56.1±12.7 μg/mL) compared with controls (2.8±1.3 μg/mL; P<0.001). For the 7 dogs that had samples collected on all 3 days, the mean CRP concentrations decreased significantly (P=0.043) over the 5 days of measurement. Of the 16 dogs with AP, 14 were discharged from the hospital and 2 were euthanized. Conclusions: Serum CRP concentrations were elevated in this group of 16 dogs with spontaneously occurring AP. In the 7 dogs that had measurements on all 3 days, the mean CRP concentration decreased from the day of diagnosis to the measurement made 5 days later.  相似文献   

Effect of plant proteins and crystalline amino acid supplementation on postprandial plasma amino acid profiles and metabolic response in rainbow trout (Oncorhynchus mykiss)     

Marine Rolland  Bodil K. Larsen  Jørgen Holm  Johanne Dalsgaard  Peter V. Skov 《Aquaculture International》2015,23(4):1071-1087

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In vitro pharmacologic effect of two endothelin-1 antagonists on equine colonic arteries and veins     

Venugopal CS  Holmes EP  Koch CE  Curtis LA  Holm AS  Moore RM 《American journal of veterinary research》2001,62(2):154-159

OBJECTIVE: To evaluate the effectiveness of 2 potential endothelin (ET)-1 antagonists in blocking the contractile responses of equine colonic vessels to increasing concentrations of ET-1. SAMPLE POPULATION: Mesenteric vessels from 6 clinically healthy horses. PROCEDURE: Colonic vessels (arterial and venous rings) were placed in organ baths with oxygenated Tyrode solution at 37 C. Each was attached to a force transducer interfaced with a polygraph, and 2 g of tension was applied and equilibrated for 45 minutes. Then, B-1 (PD 142893) and B-2 (PD 145065) ET-1 antagonists were tested. One ring from each vessel type was used as a control for determining concentration-response relationships of ET-1 (10(-10) to 10(-6)M). Three rings of each vessel type were incubated with 3 concentrations of each antagonist (10(-7), 10(-6), and 10(-5) M) for 30 minutes before ET induced contractions were determined. The maximum contractile response and pA2 values were determined. RESULTS: Vessels contracted in a concentration-dependent manner to ET-1. Arteries responded slowly but reached greater contractions. Veins responded immediately with sustained contractions. Both antagonists inhibited contractions in a concentration-dependent manner with significant differences at 10(-6) and 10(-5)M for arteries and 10(-5) M for veins. Complete blockade of contractions was observed with B-2 (10(-5)M). The pA2 values for B-1 were 8.26 and 6.82 for arteries and veins, respectively, whereas they were 8.25 and 7.21 for B-2. CONCLUSION AND CLINICAL RELEVANCE: Both antagonists effectively blocked ET-1-induced contractions of equine colonic vessels. Because B-2 is water soluble and caused complete blockade at 10(-5) M, it appears to be the preferred antagonist.  相似文献   

Pancreatic Polypeptide and Insulin-Secreting Tumor in a Dog With Duodenal Ulcers and Hypertrophic Gastritis     

Carole A. Zerbe DVM  MS    Timothy R. Boosinger DVM  PhD    J. H. Grabau DVM    J. M. Pletcher DVM  Thomas M. O''Dorisio MD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1989,3(3):178-182

A 7-year-old spayed female Cocker Spaniel was hospitalized with a history of chronic vomiting, anorexia, and weight loss. Laboratory abnormalities included leukocytosis, metabolic alkalosis, hypoglycemia, hypoproteinemia, and hyperinsulinemia. Gastroscopy and ultrasonography revealed multiple gastric masses and a possible pancreatic mass, respectively. Examination of tissues obtained at necropsy showed a pancreatic adenocarcinoma with hepatic metastasis, gastric hypertrophy, and multiple duodenal ulcers. Immunocytochemical staining of the neoplasia was positive for pancreatic polypeptide (PP) and insulin and negative for gastrin, calcitonin, adrenocorticotropic hormone (ACTH), serotonin, L-enkephalin, chromagranin, glucagon, and somatostatin. Subsequent serum gastrin and PP assays showed a fasting hypergastrinemia with a normal response of gastrin to provocative testing and extremely increased PP values. The high PP values may have resulted in the vomiting and gastrointestinal ulceration. A PP-secreting tumor has not previously been reported in the dog.  相似文献   

Multihormonal pancreatic endocrine tumor in a dog with duodenal ulcers and hypertrophic gastropathy     

T R Boosinger  C A Zerbe  J H Grabau  J M Pletcher 《Veterinary pathology》1988,25(3):237-239

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Mineral content of the ejaculate and its relationship to larger amounts and trace elements in the prostate, seminal vesicles, epididymis and testis     

W Holm  H J Schneider  M Anke 《Archiv fuer experimentelle veterinaermedizin》1971,25(5):811-815

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Disinfection of animal enclosures, pasture and soil     

W Stellmacher  J Zerbe 《Monatshefte für Veterin?rmedizin》1971,26(23):881-885

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Isolation and cryopreservation of functionally competent equine leucocytes     

Zerbe H  Castilho LF  Engelke F  Mattos RC  Schuberth HJ  Klug E  Leibold W 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2003,50(4):179-184

Sufficient numbers of functionally competent polymorphonuclear neutrophil granulocytes (PMN) seem to be of major importance during the course of equine endometritis. In this study, we wanted to establish a method for cryopreservation of functionally competent neutrophils for an intended local endometritis therapy in mares. The separation of leucocytes by hypotonic lysis of whole blood from clinically healthy mares was superior to the separation by dextrose sedimentation. After suspension of the cells in the cryoprotective solution [equine plasma with 5% (v/v) dimethyl sulphoxide (DMSO)], the leucocytes were frozen in liquid nitrogen. A temperature gradient with low cooling velocity (1 degree C/min between 4 and -70 degrees C) resulted in highest numbers of viable cells after thawing. Thawed PMN had a high phagocytic capacity for opsonized streptococci. Their ability to generate reactive oxygen species (ROS) after stimulation with a phorbol ester was even higher than that of freshly isolated PMN and was preserved up to 6 h after thawing. The results of this study indicate that cryopreservation of PMN may provide viable and functionally competent neutrophils for therapeutic use in mares susceptible to endometritis.  相似文献   

Efficacy of milbemycin oxime in the treatment of canine generalized demodicosis: a retrospective study of 99 dogs (1995-2000)     

Holm BR 《Veterinary dermatology》2003,14(4):189-195

Ninety-nine dogs diagnosed with generalized demodicosis were treated with milbemycin oxime. Cases diagnosed before two years of age were classified as juvenile-onset (53%) and those diagnosed after two years of age as adult-onset (47%). Dogs were considered cleared of mites when none could be demonstrated in scrapings and cured if no relapse was seen for 12 months. Eighty-five per cent (84/99) were cured with milbemycin oxime used for 1-6 months (mean 2.3 months) at a dosage of 0.5-1.6 mg kg-1 body weight (mean 0.75 mg kg-1). No significant difference in dosage or treatment time was seen between juvenile and adult cases. Chance of cure was significantly better in young animals (cured cases mean age 2.97 years) than in older animals (not cured cases mean age 8.02 years). Start of treatment early in the course of disease gave a significantly better chance of cure. Cases with severe pododemodicosis had less chance of a cure (9/11 not cured).  相似文献   

"Emergency splitting" of the teat canal--a therapeutic procedure in case of concurrent occurrence of acute thelitis and severe mastitis     

Korff B  Ahlers D  Zerbe H 《DTW. Deutsche tier?rztliche Wochenschrift》2003,110(7):290-294

In case of concurrent acute thelitis and severe mastitis, an immediate surgery of the stenosis ("emergency splitting") might be justified in order to improve milkability as a prerequisite for the removal of pathological milk secretion and therapy, successful mastitis therapy. The aim of this study was to compare the milkability and the clinical cure rate of mastitis between Group A (n = 19, cows with stenosis, thelitis and mastitis) and Group B (n = 19, cows with stenosis, with or without clinical mastitis, but without acute thelitis) following surgery of the papillary duct using a cutting instrument (Danish cannula model with a double cutting edge). In most cases, milkability was restored at the end of the treatment in both groups (Group A: 89.5%; Group B: 100%). In this time period nine of 19 quarters with mastitis in Group A were clinically cured (47%). Six month after the surgery, the animal owners considered the milkability as very good in 70% and 72% of the cases in Group A and Group B, respectively. However, "emergency splitting" led to a more intensive and longer postsurgical treatment. The average duration of total treatment (day of surgery plus 6 days of obligate postsurgical treatment plus additional treatment days) was 7.4 and 6.7 days in Group A and Group B, respectively. Furthermore, the higher expenditure was due to the more costly mastitis therapy. During the six month following surgery, only one cow of Group A had to be culled due to persistent mastitis and two cows because of insufficient milkability.  相似文献