Bleeding of Farmed Atlantic Cod: Residual Blood,Color, and Quality Attributes of Pre- and Postrigor Fillets as Affected by Perimortem Stress and Different Bleeding Methods     

Hanne Digre  Ulf Erikson  Ekrem Misimi  Inger Beate Standal  Lorena Gallart-Jornet  Siriporn Riebroy 《Journal Of Aquatic Food Product Technology》2013,22(4):391-411

The main objectives of the study were to (a) assess the effect of perimortem stress on blood drainage, (b) compare the efficiency of gill cutting and direct gutting as bleeding methods, and (c) compare pre- and postrigor filleting strategies for presence of residual blood in Atlantic cod fillets. Anesthetized cod had significantly higher drainage of blood compared to stressed fish. Nevertheless, the visual assessments of residual blood were not affected by stress or bleeding method. Some minor, but significant, differences between pre- and postrigor fillets were found. Stressed fish were initially less light in color than those unstressed, but after ice storage there was no noticeable difference. However, initially, the largest difference in fillet color was due to different bleeding methods. Fillets cut from fish subjected to gill cutting were lighter and less red in color than those bled by direct gutting. After 7 and 21 days of storage, the color differences observed in fresh and salted fillets, respectively, were mainly due to the different rigor status when they were filleted, indicating that fillets cut postrigor were superior to fillets cut prerigor. Prerigor filleting resulted in lower water holding capacity after ice storage in anesthetized, direct gutted cod.  相似文献   

Effects of different land use types on infiltration capacity in a catchment in the highlands of Ethiopia     

F. Yimer  I. Messing  S. Ledin  A. Abdelkadir 《Soil Use and Management》2008,24(4):344-349

Infiltration capacity is an important variable for understanding and predicting a range of soil processes. This study investigated for different slope positions the effects of forest conversion to cultivation and grazing on soil infiltration capacity. Infiltration capacity was measured in the field in each land use type using a double‐ring infiltrometer. A total of 108 soil samples (3 slope positions × 3 land use types × 4 soil profiles × 3 soil depths) were collected to determine the variables that affect infiltration capacity viz. particle size distribution, organic carbon content, dry bulk density and soil moisture content. The results showed that in the cultivated and grazed land compared with forest, infiltration capacity and soil moisture content were 70 and 45% smaller respectively, and dry bulk density was 13–20% larger. Changes in soil structure caused by surface soil compaction because of tillage and animal trampling coupled with a smaller soil organic carbon content, are likely to be the principal factors causing the decline in infiltration capacity and soil moisture content after conversion of forest to cultivation and grazing.  相似文献   

Reducing error in feline platelet enumeration by addition of Iloprost to blood specimens: comparison to prostaglandin E1 and EDTA          下载免费PDF全文

Harold W. Tvedten  Kerstin Bäcklund  Inger E. Lilliehöök 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(2):179-187

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Mosaic numbers     

Ledin G  Moore RE 《Science (New York, N.Y.)》1969,163(3868):704-705

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Frequent long-distance plant colonization in the changing Arctic     

Alsos IG  Eidesen PB  Ehrich D  Skrede I  Westergaard K  Jacobsen GH  Landvik JY  Taberlet P  Brochmann C 《Science (New York, N.Y.)》2007,316(5831):1606-1609

The ability of species to track their ecological niche after climate change is a major source of uncertainty in predicting their future distribution. By analyzing DNA fingerprinting (amplified fragment-length polymorphism) of nine plant species, we show that long-distance colonization of a remote arctic archipelago, Svalbard, has occurred repeatedly and from several source regions. Propagules are likely carried by wind and drifting sea ice. The genetic effect of restricted colonization was strongly correlated with the temperature requirements of the species, indicating that establishment limits distribution more than dispersal. Thus, it may be appropriate to assume unlimited dispersal when predicting long-term range shifts in the Arctic.  相似文献   

Two-dimensional gel electrophoresis detection of protein oxidation in fresh and tainted rainbow trout muscle   总被引：1，自引：0，他引：1  

Kjaersgård IV  Jessen F 《Journal of agricultural and food chemistry》2004,52(23):7101-7107

Protein oxidation is evaluated in rainbow trout muscle by labeling protein carbonyls with 2,4-dinitrophenyl hydrazine (DNPH) followed by immunoblotting of proteins separated by SDS-PAGE or two-dimensional gel electrophoresis (2D-GE). The carbonylation level is accessed on proteins in a whole muscle homogenate or proteins soluble in a high-salt or low-salt buffer. Spoilage-related changes in carbonylation are followed in the high-salt-protein and low-salt-protein fractions by 2D immunoblotting, which reveals increases regarding total number and intensity of carbonylation in both protein fractions for fish kept at room temperature for 48 h. The major amount of carbonylated proteins is found among the high-salt-soluble proteins, and this protein fraction is also responsible for the biggest increase in carbonylation during fish tainting. The results give an estimate of the level of protein carbonylation in rainbow trout and reveal that oxidation increases for a distinct number of proteins during tainting.  相似文献   

Identification of carbonylated protein in frozen rainbow trout (Oncorhynchus mykiss) fillets and development of protein oxidation during frozen storage     

Kjaersgård IV  Nørrelykke MR  Baron CP  Jessen F 《Journal of agricultural and food chemistry》2006,54(25):9437-9446

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Pheno- and genotyping of Staphylococcus epidermidis isolated from bovine milk and human skin     

Thorberg BM  Kühn I  Aarestrup FM  Brändström B  Jonsson P  Danielsson-Tham ML 《Veterinary microbiology》2006,115(1-3):163-172

The purpose of this study was to improve our knowledge concerning the epidemiology and strain diversity of Staphylococcus epidermidis isolated from bovine milk in commercial dairy herds. A total of 341 S. epidermidis isolates obtained from cows' milk (317), farmers (17) and patients (7) were characterized. Of these 105 isolates were from cows' milk in two farms, where also 17 isolates were sampled from farmers. The remaining 212 isolates from cows' milk were from 170 farms. All isolates were examined by antimicrobial susceptibility, whereas 202 were examined by pulsed-field gel electrophoresis (PFGE) and 122 by ribotyping. PFGE showed single patterns in the human strains with one exception; one strain was categorised as the same clone as four of the milk strains. PFGE divided 73 of the milk strains into 62 different patterns. The PFGE method had high discriminatory power and shows that many different S. epidermidis types exist in milk samples. Antibiotic resistance patterns matched the SmaI profiles closely in the two herds, but poorly in the routinely collected milk samples. Isolates from herd 1 showed one to five patterns, depending on the typing method used. Isolates from the milker's skin showed one pattern, which was identical to the most common pattern found in the milk isolates. Isolates from herd 2 showed three to four patterns, two of these being identical to skin isolates from the milker. As dairy cows are not a natural host for S. epidermidis the results suggest a human source of these udder infections.  相似文献   

Prevalence of Cryptosporidium and Giardia in free-ranging wild cervids in Norway   总被引：1，自引：0，他引：1  

Hamnes IS  Gjerde B  Robertson L  Vikøren T  Handeland K 《Veterinary parasitology》2006,141(1-2):30-41

Faecal samples were collected from 1,190 wild cervids in Norway and analyzed for cysts/oocysts of the protozoan parasites Giardia and Cryptosporidium. Samples were from calves, yearlings and adults of moose (Alces alces), red deer (Cervus elaphus), roe deer (Capreolus capreolus) and reindeer (Rangifer tarandus) shot during the hunting season. Cryptosporidium was found in 15 (3.3%) of 455 moose, 1 (0.3%) of 289 red deer, 18 (6.2%) of 291 roe deer, but was not found in any of 155 reindeer. Giardia was found in 56 (12.3%) moose, 5 (1.7%) red deer, 45 (15.5%) roe deer and 11 (7.1%) reindeer. The calves had the highest prevalence of infection, but this was only statistically significant for Giardia in moose and for Cryptosporidium and Giardia in roe deer. Calves generally had the highest intensity of infection, but this difference was only statistically significant for calves with Giarda and the highest intensity of infection. Both Giardia and Cryptosporidium were found in samples from several geographical areas, indicating that these parasites are distributed among the cervid population in all parts of Norway, especially in moose and roe deer. This is the first published report of Cryptosporidium in moose and of Giardia in reindeer.  相似文献   

Genome sequence diversity and clues to the evolution of variola (smallpox) virus     

Esposito JJ  Sammons SA  Frace AM  Osborne JD  Olsen-Rasmussen M  Zhang M  Govil D  Damon IK  Kline R  Laker M  Li Y  Smith GL  Meyer H  Leduc JW  Wohlhueter RM 《Science (New York, N.Y.)》2006,313(5788):807-812

Comparative genomics of 45 epidemiologically varied variola virus isolates from the past 30 years of the smallpox era indicate low sequence diversity, suggesting that there is probably little difference in the isolates' functional gene content. Phylogenetic clustering inferred three clades coincident with their geographical origin and case-fatality rate; the latter implicated putative proteins that mediate viral virulence differences. Analysis of the viral linear DNA genome suggests that its evolution involved direct descent and DNA end-region recombination events. Knowing the sequences will help understand the viral proteome and improve diagnostic test precision, therapeutics, and systems for their assessment.  相似文献