排序方式: 共有26条查询结果,搜索用时 109 毫秒
1.
da Rocha AC da Silva AB de Brito AB Moraes HL Pontes AP Cé MC do Nascimento VP Salle CT 《Avian diseases》2002,46(3):749-753
Sixty-three Escherichia coli strains isolated from broilers with respiratory problems were examined for virulence factors, hemolysin synthesis ability, motility, hemagglutination capacity, operon pap presence, colicin production, and serum resistance. The capacity to hemagglutinate guinea pig erythrocytes was found in 53 (84.1%) of the samples, but only 30 (47.6%) agglutinated chicken erythrocytes. D-mannose-sensitive hemagglutination against guinea pig erythrocytes was found in 19 (30.2%) samples and against chicken erythrocytes, in 15 (23.8%) samples, whereas the D-mannose-resistant hemagglutination with guinea pig erythrocytes was found in 34 (54%) samples, and 13 of these (20.6%) showed this characteristic against chicken erythrocytes. Operon pap, P fimbria codifier, was detected in 26 samples in a total of 34 D-mannose-resistant samples. Colicin production was observed in 55 (87.3%) of the strains, and 41.8% presented V colicin production. Of the samples analyzed, 56 (88.9%) presented serum resistance, six (9.5%) were intermediate, and only one (1.6%) was sensitive to the action of the complement. The diversity of virulence profiles detected in the samples in this study explains in part the multifactorial characteristics of avian colibacillosis. 相似文献
2.
Cibele Soares Pontes Patricia Pereira De Lima Maria de Fátima Arruda 《Aquaculture Research》2008,39(13):1416-1422
In shrimp farms, food partitioning during the course of the day is contradictory, ethology studies may help in determining the proper frequency. Litopenaeus vannamei juveniles were placed in 30 L aquariums (41 m−2), exposed to a 12:12 h light–dark cycle. Feeding was provided at 10% of biomass three, four and seven times per day, with observations made in 15 min h−1 windows, initiated after the ration was offered. Latency to access the feeding tray and to start eating was recorded using instantaneous focal sampling and digestive tract filling (DTF) by the continuous focal method. Weight gain was recorded at the end of the experiment. We performed seven repetitions, with 28 individuals observed for 33 days for each treatment (490 h of observation). The three-times offering induced less latency for accessing the tray and for ingesting the feed as well as a higher DTF when compared with the other frequencies. Animals fed three and four times had similar weight gains, and were greater than those fed seven times. Our results indicate that a more spaced food offering stimulates the search for and ingestion of feed. As compared with other frequencies, the three-times-per-day option assumes lower labour costs and a more efficient use of the feed. 相似文献
3.
Cesar Ricardo Arruda Fernanda Ramiro Vanessa Faria Roberto Barcelos Daniel Pontes Fernanda Passos Fabiano Kaiser Karoline dos Santos Teixeira Aline Maria Serrano Aline Abreu Leticia Siqueira Danielle Teixeira Matheus Vezzone Mariana Polivanov Helena Castilhos Zuleica 《Journal of Soils and Sediments》2022,22(2):547-558
Journal of Soils and Sediments - This study consists of the evaluation of metals acute toxicity levels taking into consideration a hypothetic scenario of dam breaking and land deposition of two... 相似文献
4.
BV Sanches JHF Pontes AC Basso CR Ferreira F Perecin MM Seneda 《Reproduction in domestic animals》2013,48(1):e7-e9
In this work, we evaluated whether embryo development and pregnancy rates would be affected by culturing bovine Bos indicus embryos in Synthetic Oviductal Fluid with amino acids (SOFaa) or G1/G2 sequential medium under a low‐oxygen atmosphere. Using Ovum Pick Up, we obtained 1,538 oocytes, divided into G1/G2 (n = 783) and SOFaa (n = 755). No difference was observed for blastocyst development among the groups (27.8% ± 14.6 and 34.9% ± 20.0 for G1/G2 and SOFaa respectively, p > 0.05). Transferring the embryos (n = 450) from both groups to recipients resulted in similar pregnancy rates for the G1/G2 (38.4% n = 78/203) compared to the SOFaa (39.7% n = 98/247). Our findings confirm that Bos indicus embryos cultured in SOFaa and G1/G2 under low‐oxygen atmosphere have similar in vitro (blastocyst rate) and in vivo (pregnancy rate) developmental capacity. However, embryos cultured in G1/G2 medium have higher cleavage than those cultured in SOFaa medium. 相似文献
5.
Baleeiro CO Paranhos-Silva M dos Santos JC Oliveira GG Nascimento EG de Carvalho LP dos-Santos WL 《Veterinary parasitology》2006,139(1-3):21-28
In this study, humoral (circulating anti-Leishmania antibodies) and cellular (Montenegro's skin test) immune responses of dogs from an endemic area of visceral leishmaniosis were tested using Leishmania chagasi, Leishmania amazonensis and Leishmania braziliensis antigens. The antibody response was tested in three animal groups, selected according to their anti-L. chagasi antibody activity, as measured by ELISA in the serum: 19 negative (O.D. below 0.30), seven with undefined (O.D. between 0.40 and 0.70) and 12 positive (O.D. above 1.0) ELISA result. In the group of animals with positive ELISA, the antibody activity against L. chagasi antigens (mean O.D.=1.31) was significantly higher (ANOVA, P<0.01) than against L. amazonensis (mean O.D.=0.88) or L. braziliensis (mean O.D.=0.87) antigens. The Montenegro's skin test results obtained with L. chagasi and L. braziliensis antigens showed a fair agreement (kappa=0.309). The same was observed when antigens from L. braziliensis and L. amazonensis were compared (kappa=0.374), whereas a moderate agreement between the results from tests performed with L. chagasi and L. amazonensis antigens was observed (kappa=0.530). The induration areas obtained with L. braziliensis antigen were smaller than those obtained with the other antigens. The data presented herein indicate that the use of antigens from different Leishmania species may interfere with the results of the immunological tests performed in dogs in an endemic area of visceral leishmaniosis. 相似文献
6.
Luciana Pontes Silva Aline Cristina Sant’Anna Lívia Carolina Magalhães Silva Mateus José Rodrigues Paranhos da Costa 《Tropical animal health and production》2017,49(1):153-162
The aim of this study was to determine whether applying good practices of handling during the pre-weaning period have long-term effects on behavioral and physiological indicators, health status, and average daily gain (ADG) of crossbred Bos taurus × Bos indicus heifer calves. During the pre-weaning period, 98 crossbred of Holstein × Gir heifer calves were allotted into three treatments: (1) good practices of handling + brushing (GPB; n?=?25), (2) good practices of handling (GP; n?=?25), and (3) control (n?=?48). Every 2 months, four evaluation periods (EV1 to EV4) were conducted to record data. Behavioral indicators comprised time to drive (TD), flight speed (FS), flight distance (FD), and composite reactivity score (CRS). Physiological indicators of acute stress during handling comprised respiratory and heart rates. Health status comprised data regarding occurrence of most common diseases (i.e., pneumonia and anaplasmosis). Collected data were analyzed by using a linear mixed model for repeated measures, Tukey’s test, and chi-squared procedures. Treatment influenced (P?<?0.05) TD, FS, and FD but not CRS (P?=?0.78). From EV1 to EV3, the control calves had the lowest TD. The GPB group had lower FS than the control but did not differ from GP. The GPB group had lower FD means than the other two groups in EV2, EV3, and EV4. No differences (P?>?0.05) due to treatment were observed on heart and respiratory rates, ADG, or occurrence of pneumonia and anaplasmosis. It was concluded that adoption of good practices of handling during pre-weaning period may lead to long-term positive effects. 相似文献
7.
Patrícia Pereira De Lima Cibele Soares Pontes Maria de Fátima Arruda 《Aquaculture Research》2009,41(1):53-60
Management practices on most Brazilian shrimp farms have resulted in high expenses for producers and damage to the environment. Applied ethology could provide information on the pattern of shrimp activity, enabling more efficient feeding frequencies. Behavioural activities of the shrimp Litopenaeus vannamei were recorded during different feeding frequencies. The shrimp were kept in aquariums on a 12:12 h light/dark cycle. They were fed commercial ration three (at 6:00, 12:00 and 18:00 hours), four (at 6:00, 10:00, 14:00 and 18:00 hours) or seven times per day (at 6:00. 8:00, 10:00, 12:00, 14:00, 16:00 and 18:00 hours). We observed animals at 15 min?1 aquarium?1 time periods, recording feeding, substrate exploration, swimming and inactivity. Feeding and exploration were the highest for shrimp fed three times per day, swimming was greatest when animals received four feedings, whereas inactivity was higher for shrimp fed seven times per day. There was greater food ingestion between 12:00 and 14:00 hours for animals fed three and four times per day, whereas swimming was high mainly at 18:00 hours for shrimp fed three and seven times per day. The results indicate higher forage‐related activities (exploration/ingestion) when feed was offered three or four times, suggesting optimization in the pattern of shrimp activities based on these feeding frequencies. 相似文献
8.
Rafael Coelho Ribeiro Walkymário de Paulo Lemos Paulo Manoel Pontes Lins Roberta de Melo Valente Antonio Agostinho Müller Alexandre Mehl Lunz José Cola Zanuncio 《Phytoparasitica》2012,40(3):239-241
The coconut palm (Cocos nucifera L., Arecaceae) has great economic and social importance for many industrial and non-industrial products. Pests can reduce its productivity and cause its death. In 2005, larvae and adults of a Curculionidae were observed damaging the floral stalk of coconut palms in commercial plantations in the municipality of Moju, Pará State, in the Brazilian Amazon. Insects were identified as the black coconut bunch weevil, Homalinotus depressus (L.) (Coleoptera: Curculionidae: Cholini). This is the first report of this pest damaging coconut palms in Brazil. 相似文献
9.
Qian W Miki D Zhang H Liu Y Zhang X Tang K Kan Y La H Li X Li S Zhu X Shi X Zhang K Pontes O Chen X Liu R Gong Z Zhu JK 《Science (New York, N.Y.)》2012,336(6087):1445-1448
Active DNA demethylation is an important part of epigenetic regulation in plants and animals. How active DNA demethylation is regulated and its relationship with histone modification patterns are unclear. Here, we report the discovery of IDM1, a regulator of DNA demethylation in Arabidopsis. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function. Our study reveals how some genes are indicated by multiple epigenetic marks for active DNA demethylation and protection from silencing. 相似文献
10.
Bisinotto RS Ibiapina BT Pontes EO Bertan CM Satrapa R Barros CM Binelli M 《Reproduction in domestic animals》2012,47(2):319-327
Follicular estradiol triggers luteolysis in cattle. Therefore, the control of follicle growth and steroidogenesis is expected to modulate luteal function and might be used as an anti‐luteolytic strategy to improve embryo survival. Objectives were to evaluate follicular dynamics, plasma concentrations of estradiol and luteal lifespan in Bos indicus and crossbred cows subjected to sequential follicular aspirations. From D13 to D25 of a synchronized cycle (ovulation = D1), Nelore or crossbred, non‐pregnant and non‐lactating cows were submitted to daily ultrasound‐guided aspiration of follicles >6 mm (n = 10) or to sham aspirations (n = 8). Diameter of the largest follicle on the day of luteolysis (7.4 ± 1.0 vs 9.7 ± 1.0 mm; mean ± SEM), number of days in which follicles >6 mm were present (2.3 ± 0.4 vs 4.6 ± 0.5 days) and daily mean diameter of the largest follicle between D15 and D19 (6.4 ± 0.2 vs 8.5 ± 0.3 mm) were smaller (p < 0.01) in the aspirated group compared with the control group, respectively. Aspiration tended to reduce (p < 0.10) plasma estradiol concentrations between D18 and D20 (2.95 ± 0.54 vs 4.30 ± 0.55 pg/ml). The luteal lifespan was similar (p > 0.10) between the groups (19.6 ± 0.4 days), whereas the oestrous cycle was longer (p < 0.01) in the aspirated group (31.4 ± 1.2 vs 21.2 ± 1.3 days). Hyperechogenic structures were present at the sites of aspiration and were associated with increase in concentration of progesterone between luteolysis and oestrus. It is concluded that follicular aspiration extended the oestrous cycle and decreased the average follicular diameter on the peri‐luteolysis period but failed to delay luteolysis. 相似文献