Flow Cytometry Identification of X- and Y-Chromosome-Bearing Goat Spermatozoa   总被引：3，自引：0，他引：3  

I Parrilla  JM Vazquez  J Roca  EA Martinez 《Reproduction in domestic animals》2004,39(1):58-60

Flow cytometric sorting technology was used to measure the difference in DNA content between X- and Y-chromosome-bearing spermatozoa in bucks. Spermatozoa were analysed by flow cytometry to characterize X- and Y-chromosome-bearing sperm populations and to quantify the DNA difference between them. Two symmetrical, overlapping and clearly separated peaks, corresponding to X- and Y-bearing spermatozoa, were detected. The difference in fluorescence intensity between the peaks was 4.4 +/- 0.03% without any significant inter- or intra-animal variations. Therefore, the identification and selection of high-purity samples of sperm populations for sex sorting is easier in bucks compared with other domestic species.  相似文献   

蛋雏鸡传染性喉气管炎的诊治     

刘文超  贾俊亮  高娟  王海刚  孙士生 《中国兽药杂志》2005,39(2):49-50

鸡传染性喉气管炎(ILT)是由鸡传染性喉气管炎病毒引起的以剧烈咳嗽、气喘、高度呼吸困难和气管内有血样渗出物等为特征的一种急性呼吸道传染病.育成蛋鸡感染该病多有报道,而蛋雏鸡感染发病比较少见.本文以一起48日龄海兰褐蛋雏鸡传染性喉气管炎病例为题材,从发病情况、临床症状、病理剖检变化、诊断、防治等几个方面对该病做了详尽阐述.  相似文献   

猪败血性链球菌与附红细胞体混合感染病例     

孙展和  贾俊亮  王海刚  徐燕 《中国兽药杂志》2005,39(5):46-47

猪败血性链球菌病是猪链球菌感染引起的一种急性败血性传染病,猪附红细胞体病是由猪附红细胞体寄生于猪红细胞而引起的一种散在的热性、溶血性传染病.本文以某猪场这2种病混合感染病例为题材,从发病基本情况、临床症状、剖检变化、实验室检验、防治措施等6个方面对该混合感染病例做了详尽的阐述.  相似文献   

Simulation study of the competitive ability of erect, semi-erect and prostrate cowpea (Vigna unguiculata) genotypes   总被引：1，自引：1，他引：1  

G WANG  ME MCGIFFEN JR  JL LINDQUIST†  JD EHLERS  & I SARTORATO‡ 《Weed Research》2007,47(2):129-139

Ecophysiological simulation models provide a quantitative method to predict the effects of management practices, plant characteristics and environmental factors on crop and weed growth and competition. The INTERCOM interplant competition model was parameterised, calibrated by monoculture data for three cowpea (Vigna unguiculata) genotypes that differed in growth habit, common sunflower (Helianthus annuus) and common purslane (Portulaca oleracea), and used to simulate competition of cowpea cover crops with sunflower or purslane. The simulation results were compared with observations from field competition experiments in 2003 and 2004. INTERCOM more accurately simulated actual field data for the competition of cowpea genotypes and sunflower than companion field experiments for the competition of cowpea and purslane. The validated simulation model of cowpea and sunflower at two densities was used to study the effects of cowpea growth habit on final biomass production of cowpea and sunflower. The model suggested that erect growth habit was more competitive than semi‐erect and prostrate growth habit, when cowpea genotypes were grown with sunflower. Cowpea leaf area distribution was important to higher cowpea biomass production, while cowpea height growth was important to reduce sunflower biomass. Our simulation approach is suggested as a method for crop breeders to gauge the likely success of selection for competitive crops before undertaking expensive long‐term breeding experiments.  相似文献   

Failure of passive transfer in foals: incidence and outcome on four studs in New South Wales     

CM TYLER-McGOWAN  JL HODGSON  DR HODGSON 《Australian veterinary journal》1997,75(1):56-59

Objective To determine the regional incidence and effectiveness of treatment of failure of passive transfer (FPT) in foals. Design A study of disease incidence. Animals Eighty-eight foals and 57 mares from four studs in the practice area of the Rural Veterinary Centre were tested. Procedure Foals were tested for their serum IgG and total serum protein (TSP) concentration within the first 72 hours of life. Colostrum was collected from mares and specific gravity determined. FPT and partial failure of passive transfer (PFPT) of immunoglobulins was diagnosed when serum IgG concentrations were < 4 g/L and 4 to 8 g/L respectively. Owners of foals diagnosed with FPT were offered treatment with 1 to 2 L plasma (TSP > 70 g/L); 9 (64%) of the affected foals were treated. Results Fourteen foals (16%) had FPT whereas 15 (17%) had PFPT. There were significant differences between the mean TSP concentration in foals with FPT (42.6 ± 4.2 g/L), PFPT (48.1 ± 3.9 g/L) and those acquiring adequate passive immunity (58.9 ± 5.5 g/L) (P < 0.01). Sixteen (29%) mares had pre-suck colostral specific gravity < 1.060 and 12 (71%) foals raised by these mares had FPT or PFPT. The incidence of severe disease (categorised by a sepsis score > 11, positive culture of bacteria from blood or disease requiring hospitalisation) in all foals in the first 2 months of life was 10%. However, none of the nine foals with FPT that received plasma experienced severe disease. In contrast, foals with PFPT had an increased susceptibility to severe disease (P < 0.001) when compared with normal foals. Conclusion Treatment of foals with FPT may reduce the subsequent incidence of severe disease. Pre-suck colostral specific gravity and foal TSP may be used to predict the likelihood of FPT and PFPT. Even though the number of foals studied is small the results highlight the importance of optimal management practices in reducing the incidence of FPT and disease associated with this process.  相似文献   

Walnut demonstrates strong genetic variability for adaptive and wood quality traits in a network of juvenile field tests across Europe     

Fady  B.  Ducci  F.  Aleta  N.  Becquey  J.  Diaz Vazquez  R.  Fernandez Lopez  F.  Jay-Allemand  C.  Lefèvre  F.  Ninot  A.  Panetsos  K.  Paris  P.  Pisanelli  A.  Rumpf  H. 《New Forests》2003,25(3):211-225

Adaptive and wood quality trait data were collected and analyzed on commercially available Juglans regia and J. regia×J. nigra provenances and progenies planted across Europe in a multi-site network. A total of 19 seed sources, replicated 35 times per site, were planted at 13 sites from 5 European countries, encompassing the potential distribution area of timber production plantation sites. The following traits were evaluated: survival, height, diameter at breast height, stem form, apical dominance, vegetative budbreak, along with biotic and abiotic damage. Mean values were significantly different both among provenances/progenies and sites. Most common damage was late spring and early autumn frost. Bud break ranking was significantly correlated with provenance and progeny origin. Although J. regia is fast growing, southern European early budbreak plant material should not be planted under most middle European conditions where late spring frost can be expected, as it has a significant negative impact on architectural (and thus wood quality) traits. Hybrid J. regia×J. nigra progeny performed better than J. regia provenances/progenies for most traits measured. Differences were significantly in favor of hybrids at sites with medium to low fertility, although some locally selected seed sources tended to perform as well as hybrids on high fertility sites.  相似文献   

Immunohistochemical Expression of Growth Factors in the Follicular Wall of Normal and Cystic Ovaries of Sows          下载免费PDF全文

FJF Sant'Ana  JL Reis Junior  GR Blume  EJ Gimeno  F Rey  HH Ortega 《Reproduction in domestic animals》2015,50(2):327-332

The expression of growth factors was evaluated immunohistochemically in normal and cystic ovaries of sows. The immunohistochemically stained area (IHCSA) was quantified by image analysis to analyse the expression of these proteins in the follicular wall of secondary, tertiary and cystic follicles. IGF‐I immunoreactivity was strong in the granulosa cell layer (GC), moderate in the theca interna (TI) and mild in the theca externa (TE) of the normal follicles. There was severe reduction of the labelling to IGF‐I in the GC of the follicular and luteinized cysts. In the normal follicles, the reactivity for IGF‐II was very similar to pattern noted in IGF‐I. There was reduction of the IHCSAs in the GC of the follicular and luteinized cysts, but the decrease was not significant. The staining of the IGF‐II in the TI and TE of the cysts was increased, in comparison with normal follicles. The IHCSAs for VEGF were higher in the GC and TE of the normal follicles in contrast to TI, but this difference was noted only in the tertiary follicle. The VEGF reactivity increased in the GC of the cysts, in relation to normal follicles. The results of the current study show that the formation of ovarian cysts in sows is associated with alterations in the immunohistochemical expression of some growth factors.  相似文献   

Gustatory sensitivity of the external taste buds of Oreochromis niloticus L. to amino acids     

S Y Yacoob  K Anraku  T Marui  T Matsuoka  G Kawamura  & M Vazquez Archdale 《Aquaculture Research》2001,32(3):217-222

The gustatory sensitivity of the Nile tilapia, Oreochromis niloticus L., to different amino acids was studied using an electrophysiological approach. The electrical responses were recorded from a branch of the facial nerve innervating the external taste buds of the upper lip. The relative stimulatory effectiveness (RSE) of nine amino acids and betaine were determined at a concentration of 1 mm and all of them elicited neural responses. This species responded well to the neutral, basic and acidic amino acids. The most stimulatory amino acids were L‐histidine, L‐arginine, L‐serine, L‐methionine and L‐glutamine; L‐proline and betaine were the least stimulatory. The results of this study suggest that the Nile tilapia has high external gustatory sensitivity to some amino acids as a physiological adaptation to search effectively for their sources. The effect of the pH, ranging from 4.0 to 9.0, on the RSE of three neutral amino acids and artificial pond water (APW) was also studied. The RSE increased below pH 6.0 and was relatively unaffected from 7.0 to 9.0, indicating that acidified stimulants are highly stimulatory in this species. Nile tilapia did not discriminate the pH of APW as effectively as some of the species studied earlier.  相似文献   

Collaborative study of a new method for extraction of light filth from reconditioned (coarsely ground) nutmeg     

A W Vazquez 《Journal of the Association of Official Analytical Chemists》1971,54(3):575-578

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Estudio Histoquímico de las Fibras Musculares de Algunos Teleósteos Marinos y de Agua Dulce     

G. Ramirez  Zarzosa  F. Gil    F. Moreno    J. Ma.  Vazquez R. Latorre 《Anatomia, histologia, embryologia》1991,20(2):169-179

Histochemical and morphological studies of skeletal muscle of some fresh and salt water fish is presented. Distribution of three muscle types, red, intermediate and white, is discussed.  相似文献