Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors     

Byoung-Joo SEO  Hyunil KIM  Ho-Seong CHO  Byoung-Yong PARK  Won-Il KIM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):133-138

Two commercial PRRSV ELISA kits (IDEXX and Bionote) were evaluated for their sensitivity and specificity using 476 PRRS-positive serum samples collected from 7 animal challenge experiments and 1,000 PRRS-negative sera. Both ELISA kits exhibited 100% sensitivity with sera collected 14 to 42 days post-infection, and the results from the kits were highly correlated (R²=0.9207). The specificity of IDEXX or Bionote kit was 99.9% or 99.7%, respectively. In addition, the Bionote ELISA kit was used to examine 100 sera that were determined to be falsely positive either by IDEXX 2XR or 3XR ELISA, and only 7 of these samples were found to be positive. These results indicate that both ELISA kits exhibited similar levels of sensitivity and specificity and would complement one another for the verification of false-positive samples.  相似文献   

Setaria digitata was the main cause of equine neurological ataxia in Korea: 50 cases (2015–2016)     

Hyunkyoung LEE  Hyeshin HWANG  Younghye RO  Ji-Hyeon KIM  Kyunghyun LEE  Eunjin CHOI  Youchan BAE  Byungjae SO  Inhyung LEE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):869

This study was performed to examine and clarify the cause of hindlimb ataxia and neuropathy seen in the South Korean horse population. Fifty horses diagnosed with hindlimb ataxia and neuropathy were referred for this study. Neurological examination was performed on 47 horses while necropsy was performed in all 50 animals. The occurrence of neurological diseases increased rapidly in the summer and 47 out of 50 horses were referred after the end of July. The incidence of neurological diseases started from the southern part of Korea in July and proceeded northward in August and September. Although there was no correlation with age, Thoroughbred and Warmblood horses showed a higher incidence rate than Halla and Jeju horses. The incidence rate was 5 times higher in geldings than in mares and stallions. Of the 20 cases, 16 were diagnosed with eosinophilic meningoencephalomyelitis in 2015. The most common lesions observed in 2016 were parasitic meningoencephalomyelitis (10 cases, 33%) and eosinophilic meningomyelitis (7 cases, 23%). Histopathological analysis of the brain and spinal cord revealed nematodes of approximately 100–200 µm in diameter, microcavitation and infiltrates of eosinophils, and brown pigmented macrophage infiltrates. The nematodes were identified as Setaria digitata via DNA sequencing, performed subsequent to polymerase chain reaction using DNA isolated from formalin-fixed paraffin-embedded tissue sections of the spinal cord. These results show that aberrant migration of Setaria digitata larva in the brain and spinal cord was a major cause for neurological signs in horses.  相似文献   

Single oral β-cryptoxanthin administration increases its serum concentration and enhances peripheral blood neutrophil function in Holstein cattle     

Atsushi KIMURA  Yo-Han KIM  Kazuyoshi HASHIZUME  Akira ITO  Katsuyuki MUKAI  Keiichiro KIZAKI  Shigeru SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):829

We investigated the effect of oral administration of β-cryptoxanthin (β-CRX) on its serum concentration and peripheral neutrophil functions by the chemiluminescence (CL) response in Holstein cattle. A single oral administration of β-CRX was performed for serum β-CRX concentration (0, 0.05, 0.1, or 0.2 mg/kg body weight [BW]) and for peak CL response of peripheral neutrophils (0.2 mg/kg BW). The serum β-CRX concentration was peaked on 2 days after, similar to peak CL response on 3 days after β-CRX administration. Therefore, a single oral administration of β-CRX (0.2 mg/kg BW) induces higher serum concentration and concurrently enhances bactericidal ability of peripheral neutrophils in Holstein cattle.  相似文献   

Comparison of intraoperative cardiorespiratory and behavioral responses to medetomidine combined with tramadol or butorphanol during standing laparoscopic ovariectomy in horses     

Ahram KIM  Naoki SASAKI  Inhyung LEE  Jong-pil SEO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):643

The purpose of this study was to assess the cardiorespiratory and behavioral responses to the combination of medetomidine and tramadol (M-T) or butorphanol (M-B) in standing laparoscopic ovariectomy in horses. One ovary was removed under M-T and the contralateral ovary was removed under M-B with at least 4 weeks between operations at random. Horses were sedated using intravenous medetomidine (5 µg/kg) followed by tramadol (1 mg/kg) or butorphanol (10 µg/kg) after 5 min. Sedation was maintained through the repeated injection of medetomidine (1 µg/kg) and tramadol (0.4 mg/kg) or medetomidine (1 µg/kg) and butorphanol (4 µg/kg) every 15 min. Cardiorespiratory function and behavioral responses, including, sedation, ataxia, and analgesia, were assessed during the surgery. There were no significant differences in cardiorespiratory values and sedation and analgesia scores between M-T and M-B. Ataxia scores were significantly lower in M-T than in M-B. This result suggests that M-T could maintain smooth and stable standing surgery with minimal cardiorespiratory changes in horses.  相似文献   

Evaluation of left ventricular function with cardiac magnetic resonance imaging and echocardiography after administration of dobutamine and esmolol in healthy beagle dogs     

Hyeryoung JEONG  Hanbin LEE  Jaihyun JUNG  Hyunryung KIM  Jin YU  Hyounglok YOON  Youngjae LEE  Jinhwa CHANG  Dongwoo CHANG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):581

Unlike echocardiography, cardiac magnetic resonance imaging (cardiac MRI) results in a near-exact assessment of cardiac structures and function. However, most veterinary studies have focused on dogs with normal cardiac function. We hypothesized that there would be significant differences in cardiac measurements between cardiac MRI and echocardiography when left ventricular (LV) function was abnormal. This study was undertaken to compare measurements of LV function produced by cardiac MRI and echocardiography in dogs whose LV function was altered by pharmacological agents. This study was conducted with six healthy beagle dogs. We increased left ventricular contractility by administration of dobutamine; we decreased cardiac contractility with esmolol. Stroke volume measurements were made by using both cardiac MRI and echocardiography under seven different conditions with general anesthesia: control, three doses of esmolol (100, 200, and 500 µg/kg/min), and three doses of dobutamine (10, 20, and 50 µg/kg/min). Experiments involving each condition were conducted at least 1 week apart. When LV contractility was normal, ejection fraction (EF) and stroke volume (SV), as measured by echocardiography and cardiac MRI, were not significantly different. However, when contractility was changed by pharmacological agents, EF and SV were overestimated by echocardiography, compared to MRI. Evaluation of cardiac function in patients treated with pharmacological agents should be conducted carefully because EF and SV measured by echocardiography can be overestimated, compared with EF and SV obtained by cardiac MRI.  相似文献   

枯草杆菌发酵对何首乌抗氧化成分及肝癌细胞抗增殖作用的影响          下载免费PDF全文

范琼  赵敏  金明坤  韩丙军 《南方农业学报》2020,51(8):1932-1938

[目的]探讨何首乌经枯草杆菌发酵后主要抗氧化成分及其对肝癌细胞抗增殖作用效果的变化,为进一步研究何首乌发酵炮制方法及开发何首乌产品提供科学参考.[方法]将生何首乌用枯草杆菌发酵处理后,测定生品和发酵品的游离型蒽醌、结合型蒽醌和总多酚含量等成分的变化,并对比何首乌生品和发酵品对肝癌细胞HepG2抗增殖作用的变化.[结果]何首乌生品和发酵品总游离型蒽醌含量分别为1.56和1.60 mg/g,总结合型蒽醌含量分别为1.24和0.79 mg/g,何首乌经枯草杆菌发酵后结合型蒽醌含量极显著下降(P<0.01,下同).何首乌生品和发酵品水提取液的总多酚含量分别为44.88和74.01 mg/g,乙醇提取液的总多酚含量分别为61.84和93.86 mg/g,何首乌经枯草杆菌发酵后总多酚含量极显著增加.0.025 g/L何首乌生品对肝癌细胞HepG2作用24 h后的细胞抑制率为4.2%,0.25 g/L何首乌生品对肝癌细胞HepG2作用72 h后的细胞抑制率为-11.1%(负值表示促进细胞生长),即随着何首乌生品浓度增加和作用时间延长,其对肝癌细胞生长的作用由抑制转变为促进.经枯草杆菌发酵后,0.025 g/L何首乌发酵品对肝癌细胞HepG2作用24 h后的细胞抑制率为9.9%,0.25 g/L何首乌发酵品对肝癌细胞HepG2作用72 h后的细胞抑制率为20.9%,即随着何首乌发酵品浓度增加和作用时间延长,其对肝癌细胞HepG2有明显的抗增殖效果.[结论]枯草杆菌发酵可降低何首乌的毒性,增强其对肝癌细胞HepG2的抗增殖作用,表明使用枯草杆菌发酵何首乌的炮制方法是一种安全有效的炮制减毒方法,对开发相应何首乌炮制品有重要意义.  相似文献   

Effect of casein substitution with fishmeal, soybean meal and crustacean meal in the diet of the abalone Haliotis discus hannai Ino     

S.H. CHO  J. PARK  C. KIM  & J.-H. YOO 《Aquaculture Nutrition》2008,14(1):61-66

The effect of dietary substitution of casein with fishmeal, soybean meal and crustacean meal on the growth of the abalone Haliotis discus hannai Ino was determined. A 350 g casein per kilogram diet was included into the CS diet. The whole casein was then substituted by: (1) 300 g fishmeal and 200 g soybean meal per kilogram diet (FS), (2) 200 g fishmeal, 200 g soybean meal and 130 g krill meal per kilogram diet (FSK), (3) 200 g fishmeal, 200 g soybean meal and 280 g red crab meal per kilogram diet (FSC) or (4) 200 g fishmeal, 200 g soybean meal and 130 g shrimp head meal per kilogram diet (FSS). In addition, a 50‐g by‐product of green tea per kilogram diet was included in the FS diet to form the FSG diet. Sea tangle (ST)diet was supplied to abalone as a control feed. Weight gain, final shell length and final shell width of abalone fed with the various substitution feeds (FS, FSK, FSC, FSS and FSG) were not different from those obtained with the CS diet. All the formulated feeds, however, produced higher weight gain and final shell width values than the ST diet. The results of this study show that casein can be replaced with a combination of fishmeal, soybean meal, krill meal, crab meal and/or shrimp head meal in the diet without a retardation of growth of abalone.  相似文献   

大豆蛋白-多糖复合物结构与性能及其稳定性研究   总被引：1，自引：0，他引：1  

郑环宇  孔洋  郑丽  金技峰  李杨  滕飞 《农业机械学报》2022,53(6):406-415

以大豆分离蛋白（SPI）、阿拉伯树胶（GA）及卡拉胶（CA）为原料,经物理混合成功制备不同多糖与SPI复合物,考察不同添加量（SPI与GA/CA质量比为20、15、10、5）对蛋白-多糖复合物结构、性能变化趋势及其乳液稳定性的影响规律,最终探明蛋白与不同多糖复合物的相互作用机制;运用红外光谱、荧光光谱及电子显微镜解析不同复合物的结构特征,采用乳化特性、粒径、Zeta-电位、表面疏水性等指标明确不同复合物的理化特性,并通过探讨不同复合物乳液的乳化活性、乳化稳定性、表观粘度及乳析指数明晰其稳定特性。结果表明,两种多糖在酸性条件下可与SPI生成复合物,并且当SPI与CA质量比为20时,复合物的Zeta-电位最高,为（20.47±0.82） mV,平均粒径最小,为（1.37±0.01）μm,分布均匀,乳化活性指数最高,为（106.46±4.75） m²/g,乳化稳定性指数为（145.33±8.53） min,此时复合乳液的稳定性较好,CA的加入显著降低SPI内源荧光强度并改变SPI的二级结构,SPI与CA结合形成了稳定的复合物。  相似文献   

Evaluation of immobilizing agents as soil quality conditioners in addition to their metal(loid) immobilizing effect     

Chaw Su LWIN  Mina LEE  Young-Nam KIM  Gary OWENS  Kwon-Rae KIM 《土壤圈》2022,32(2):307-316

In trace metal (TM)-contaminated agricultural soils, management of TM availability is important for safe crop production. In addition, maintenance or improvement of soil quality is vital for sustainable crop cultivation. Decreased TM phytoavailability and increased soil quality can be achieved by the application of various immobilizing agents to soil, which can supply both macronutrients and organic matter. This study investigated the long-term influences of four common immobilizing agents on so...  相似文献   

Effect of Omentectomy on Adhesion Formation in Horses     

K. LEANN KUEBELBECK DVM    DONNIE E. SLONE DVM  MS  Diplomate ACVS  KIM A. MAY DVM 《Veterinary surgery : VS》1998,27(2):132-137

Objective —To determine if omentectomy would decrease the frequency of postoperative intraabdominal adhesions. Study Design —Retrospective study. Animals or Sample Population—44 horses that had either two ventral median celiotomies or a ventral median celiotomy and a necropsy more than 4 days later; 19 of these horses had their omentum removed at the initial surgery. Methods —Data retrieved from the records included location and type of intraabdominal adhesions; location of the surgical lesion; relationship of adhesions to the surgical lesion; surgical procedures; duration of initial surgery; time interval between procedures; age, gender, and breed of the horse; and clinical outcome. Fisher's exact test was used to evaluate the association between categorical explanatory and outcome variables. The effect of potential risk factors on the incidence rate of adhesion formation was estimated using a proportional hazards regression model. Results —Of 25 horses in the nonomentectomy group, 15 (60%) had postoperative adhesions that resulted in the need for a second surgical intervention, whereas of 19 horses that had omentectomy initially, only 4 (21%) had postoperative adhesions that required a second procedure. Rate of adhesion formation was higher in horses that did not have omentectomy initially (incidence ratio rate [IRR], 0.46; 90% confidence interval [CI], 0.18 to 1.19). At initial surgery, 24 horses had a small intestinal lesion, and 20 horses had a large intestinal lesion. Fifteen horses (63%) with small intestinal lesions subsequently developed adhesions compared with four horses (20%) with an initial large intestinal lesion (P= .006). At the second procedure, small intestine lesions were identified in 32 horses and large intestine lesions in 12 horses (1 horse had both small and large intestine lesions), and 1 horse had a gastric lesion. Adhesions were identified as the cause of colic signs in 19 (61%) horses with small intestinal lesions and in none of the horses with large intestine lesions. The frequency of adhesion development leading to colic associated with only the small intestine at the second surgery or necropsy was significantly greater (P= .001) than the frequency only in the large intestine. Conclusions —Omentectomy reduced the rate of postoperative adhesion formation. Adhesions are more likely to occur after small intestinal surgery and if they do occur likely involve the small intestine. Clinical Relevance —Omentectomy is a safe procedure and should be considered prophylactically for reduction of adhesion formation after abdominal surgery in horses.  相似文献