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甘蔗黑穗病菌拮抗菌HAS的筛选和鉴定 总被引：1，自引：0，他引：1
采用传统形态学鉴定及16S rRNA基因序列分析,筛选鉴定对甘蔗黑穗病菌具有拮抗作用的细菌的归属,并采用细菌的通用引物P0、P6扩增16S rRNA基因片段.结果表明:得到1 533 bp的DNA片段,其序列与枯草芽孢杆菌序列的同源性高达99％.其形态学特征:菌体为短杆状,能运动；革兰氏阳性反应；3％KOH溶解性实验呈阴性反应；有鞭毛,鞭毛周生；芽孢中生,椭圆形,孢囊稍膨大,初步将该菌株归属为枯草芽孢杆菌.与病原真菌对峙培养的结果表明,该菌对引起甘蔗和其它作物病害的多个植物病原真菌均具有很好的抑制作用.据此,可将分离的菌株HAS确定为对甘蔗黑穗病菌有较好防治效果的枯草芽孢杆菌. 相似文献
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Lymphoma is the most common malignant neoplasia in domestic ferrets, Mustela putorius furo. However, imaging findings in ferrets with lymphoma have primarily been described in single case reports. The purpose of this retrospective study was to describe imaging findings in a group of ferrets with confirmed lymphoma. Medical records were searched between 2002 and 2012. A total of 14 ferrets were included. Radiographs (n = 12), ultrasound (n = 14), computed tomography (CT; n = 1), and magnetic resonance imaging (MRI; n = 1) images were available for review. Median age at the time of diagnosis was 5.2 years (range 3.25–7.6 years). Clinical signs were predominantly nonspecific (8/14). The time between the first imaging study and lymphoma diagnosis was 1 day or less in most ferrets (12). Imaging lesions were predominantly detected in the abdomen, and most frequently included intra‐abdominal lymphadenopathy (12/14), splenomegaly (8/14), and peritoneal effusion (11/14). Lymphadenopathy and mass lesions were typically hypoechoic on ultrasound. Mild peritoneal effusion was the only detected abnormality in two ferrets. Mild pleural effusion was the most common thoracic abnormality (3/12). Expansile lytic lesions were present in the vertebrae of two ferrets with T3‐L3 myelopathy and the femur in a ferret with lameness. Hyperattenuating, enhancing masses with secondary spinal cord compression were associated with vertebral lysis in CT images of one ferret. The MRI study in one ferret with myelopathy was inconclusive. Findings indicated that imaging characteristics of lymphoma in ferrets are similar to those previously reported in dogs, cats, and humans. 相似文献
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高粱在盐胁迫下特定蛋白的表达及与耐盐性关系的研究 总被引：9，自引：0，他引：9
报道了通过统计学上逐步回归的方法,筛选出对植物耐盐性有显著贡献的蛋白质,并应用方差分析探讨上述蛋白质的最佳诱导条件,以便探明盐胁迫一特定蛋白一耐盐性之间的关系。结果表明: 由NaCl诱导的高粱耐盐性是众多蛋白质综合作用的结果,其中不受盐诱导的15. 5 kD根蛋白、受盐诱导的71. 4 kD叶蛋白对高粱耐盐性具有显著正贡 相似文献
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The effects of diet supplemented with Lactobacillus rhamnosus on tissue parameters of rainbow trout,Oncorhynchus mykiss (Walbaum) 
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下载免费PDF全文 Natalija Topic Popovic Ivancica Strunjak‐Perovic Roberta Sauerborn‐Klobucar Josip Barisic Margita Jadan Snjezana Kazazic Inga Kesner‐Koren Andreja Prevendar Crnic Jelena Suran Blanka Beer Ljubic Vesna Matijatko Rozelindra Coz‐Rakovac 《Aquaculture Research》2017,48(5):2388-2401
This study was carried out to establish the effects of a 6 week treatment with the diet supplemented with L. rhamnosus in concentrations of 107 CFU g?1 (G1 group) and 108 CFU g?1 (G2 group) on the condition expressed by condition factors (Fulton's, Clark's and B), intestinal microbiology, haematological, histological and selected antioxidative parameters of rainbow trout. A significantly higher condition factors were found in G1 group indicating that higher concentration of probiotic (108 CFU g?1) did not result in the better condition. Cholesterol and urea levels were significantly higher in both G1 and G2 groups, albumin in G1 and creatinine in G2 group with respect to control. A significantly higher liver TBARS level was observed in G2 group. The feeding with supplemented probiont apparently changed the resident microbiota. Three weeks after withdrawal of the supplemented feed, the microflora mostly reverted to the control composition, although L. rhamnosus in faecal matter of fish remained inherent. The epithelial structure of the proximal and distal intestine revealed the increased absorptive area in both treated groups, as well as the increase in the mucin‐secreting goblet cells. The L. rhamnosus‐treated groups demonstrated the capacity for the augmentation of the innate host defence. 相似文献
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IMAGING DIAGNOSIS—MAGNETIC RESONANCE IMAGING OF A NEURONAL HETEROTOPIA IN THE BRAIN OF A CAT 下载免费PDF全文
下载免费PDF全文 Antonia DeJesus Bradley J. Turek Evelyn Galban Jantra Ngosuwan Suran 《Veterinary radiology & ultrasound》2018,59(2):E17-E21
A domestic shorthair kitten was presented for evaluation and further treatment of seizures. Magnetic resonance imaging of the brain revealed a large multilobulated mass in the third ventricle extending into the right lateral ventricle with secondary obstructive hydrocephalus. The mass was homogeneously isointense to gray matter on T2W, T2‐FLAIR, T2*W, T1W, and ADC images, and hyperintense on DW‐EPI. There was no appreciable contrast enhancement. Seizures were managed medically and with subsequent ventriculoperitoneal shunt placement. Clinical status later deteriorated and the cat was euthanized. Histopathology confirmed that the mass was the result of neuronal heterotopia. To the authors’ knowledge this is the first report of neuronal heterotopia in a cat. 相似文献
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Emily B. Elser Wilfried Mai Jennifer A. Reetz Vince Thawley Hadley Bagshaw Jantra N. Suran 《Veterinary radiology & ultrasound》2020,61(4):399-408
Abdominal radiographs are commonly used in dogs and cats that present with gastrointestinal signs. When initial abdominal radiographs are equivocal for the presence or absence of gastrointestinal mechanical obstruction, follow‐up abdominal radiographs may be recommended. Based on our review of the literature, no published study has been performed to evaluate the clinical utility of serial abdominal radiographs in such cases. The purpose of this study is to determine whether follow‐up abdominal radiographs increase diagnostic accuracy for mechanical obstruction. A prospective cohort study was performed on client‐owned dogs and cats with clinical concern for gastrointestinal mechanical obstruction and initial abdominal radiographs inconclusive for the presence of obstruction. Follow‐up abdominal radiographs were performed between 7 and 28 h of the initial radiographs; an abdominal ultrasound performed within 3 h of the follow‐up study served as the gold standard. A total of 57 patients (40 dogs and 17 cats) were recruited; 19 of 57 cases (11 dogs; 8 cats) were mechanically obstructed, all with nonradiopaque foreign bodies. Four blinded reviewers (2 radiologists, 1 radiology resident, 1 criticalist) separately assessed the initial and the combined initial/follow‐up radiographic studies for diagnosis of mechanical obstruction; for each observer, there was no significant change in accuracy (P = .058‐.87) for the diagnosis of mechanical obstruction. Given the lack of significant increase in diagnostic accuracy using follow‐up radiographs in cases of occult gastrointestinal mechanical obstruction, other diagnostic options (eg, abdominal ultrasonography) could be considered when survey abdominal radiographs are inconclusive for the diagnosis of mechanical obstruction in dogs and cats. 相似文献
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长期实践证明:化学防治农作物害虫会带来药物残留且会对环境造成不同程度的污染,而生物防治效果更为环保、安全。虫生真菌作为生物防治的重要手段可以在保护原有生态环境的基础上采用一定生物手段合理防控害虫,不仅能促进农业生产的可持续发展,同时保障了我国食品绿色、安全,近年来在防治农作物害虫上发挥了出色的作用。本文分别介绍虫生真菌代表种:白僵菌、绿僵菌、拟青霉、轮枝菌等菌落特征及防治对象,从致病机理和毒力进行综合阐述,并围绕了真菌制剂产品特点、产品创制以及开放利用,系统总结虫生真菌在我国农业生产中的应用进展,旨在为我国的生态安全以及虫生真菌的开发及应用提供参考。 相似文献
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Wilfried Mai Jantra N. Suran Ana V. Cáceres Jennifer A. Reetz 《Veterinary radiology & ultrasound》2013,54(4):343-350
Renal dual‐phase computed tomograpic angiography (CTA) is used to assess suitability of feline donors prior to transplantation. A prerequisite for successful CTA is optimal synchronization between the arterial passage of contrast material and CT data acquisition. This retrospective study was conducted to compare quality of renal vascular enhancement at dual‐phase CTA in normal cats between two techniques of timing of data acquisition: the timing‐bolus and the bolus tracking method. Nine cats were scanned using the timing‐bolus technique and 14 with the bolus tracking technique using otherwise similar scanning parameters in a 16‐slice multidetector row CT scanner. The quality of enhancement of the renal vessels at the scanned arterial phase and venous phase was assessed both subjectively and objectively by three board‐certified radiologists. Arterial enhancement was not observed at the scanned arterial phase in three of the nine cats with the timing‐bolus technique but only 1 of the 14 cats with the bolus tracking technique. Early venous enhancement at the scanned arterial phase was common with the bolus tracking technique. Data acquisition was significantly faster with the bolus tracking technique. We conclude that the bolus tracking technique is a valid technique that could be integrated into the routine protocol for 16‐detector row CT renal angiography in cats. 相似文献