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Chaleow Salakij Jarernsak Salakij Kreangsak Prihirunkit Nual‐Anong Narkkong Decha Pitakkingthong 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2010,39(2):193-198
Background: The Leopard Cat (Prionailurus bengalensis) is the most frequently encountered wild cat in most of Southeast Asia. Limited hematologic investigation exists for this species. Objectives: The objectives of this study were to assess routine hematologic measurements and parameters and characterize the morphology, cytochemical staining, and ultrastructural features of blood cells in Leopard Cats. Methods: Blood samples were collected from 12 adult healthy captive Leopard Cats (7 males and 5 females). Complete blood counts were performed using an automated hematology analyzer and manual differential counts. Cytochemical staining (Sudan black B [SBB], peroxidase [PO], periodic acid‐Schiff [PAS], α‐naphthyl acetate esterase [ANAE], and β‐glucuronidase [BG]) and scanning and transmission electron microscopy were performed using standard methods. Results: Median (range) hematologic results were as follows: PCV 0.46 L/L (0.30–0.55 L/L), hemoglobin 136.5 g/L (100–183 g/L), WBC 9.0 × 109/L (6.9–15.2 × 109/L), band neutrophils 0.07 × 109/L (0–0.30 × 109/L), segmented neutrophils 2.9 × 109/L (1.2–6.34 × 109/L), lymphocytes 5.3 × 109/L (2.7–8.1 × 109/L), eosinophils 0.14 × 109/L (0–0.73 × 109/L), basophils 0/L (0–0.22 × 109/L), and monocytes 0.08 × 109/L (0–0.30 × 109/L). Neutrophils stained strongly positive for SBB, PO, and PAS; lymphocytes had fine granular positivity for ANAE and BG; monocytes were weakly positive for ANAE and BG; and basophils were strongly positive for BG. Ultrastructurally, eosinophils contained many large rod‐shaped granules with prominent crystalloid core structures, ribosomes, and mitochondria. Basophils contained many round to oval specific granules with homogeneous contents. Low number of basophils contained a few small vacuoles that usually were not detected by light microscopy. Conclusion: These findings will facilitate interpretation of hematologic results for future investigative and diagnostic studies of this species. 相似文献
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Salakij C Salakij J Narkkong NA Sirinarumitr T Pattanarangsan R 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(1):31-41
BACKGROUND: The flat-headed cat (Prionailurus planiceps) is a small wild cat of Southeast Asia and is considered extremely endangered. Little is known about the hematologic values, blood cell morphology, or hemoparasites of this species in relation to other Felidae. OBJECTIVES: The objective of this study was to report basic hematologic values and describe the light microscopic, cytochemical, and ultrastructural characteristics of blood cells in 2 wild-caught flat-headed cats. In addition, molecular analysis was done of a Hepatozoon organism found in the neutrophils of both cats. METHODS: Blood samples were collected into EDTA from the cephalic vein. A CBC, manual differential count, manual reticulocyte count, cytochemical stains (Sudan black B [SBB], alpha-naphthyl acetate esterase [ANAE], and beta-glucuronidase), and scanning and transmission electron microscopy were done using standard methods. RESULTS: HCT was slightly lower and reticulocyte counts and red cell distribution width were higher than the expected values for other species of cats. Hepatozoon organisms were found in the cytoplasm of neutrophils in both cats, but the number of infected neutrophils was very low (1%-2%). Neutrophils stained strongly positive for SBB, but were negative for ANAE and beta-glucuronidase. Hepatozoon-infected neutrophils were negative for SBB, but focally positive for ANAE and beta-glucuronidase. By transmission electron microscopy, gamonts of Hepatozoon sp were observed in neutrophils, and rarely free in plasma. Infected neutrophils had fewer specific granules and more mitochondria compared with noninfected neutrophils. PCR products of partial 18S rRNA revealed that the isolate of Hepatozoon in the flat-headed cats was closely related to that of the frog Hepatozoon sp. CONCLUSIONS: These results add to our understanding of hematologic values and blood cell morphology in Hepatozoon-infected flat-headed cats as well as the molecular analysis of the Hepatozoon organism, and may be useful for the health management and evaluation of hemoparasitic disease in this species. 相似文献
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Conventional electron microscopy and enzyme-cytochemistry were applied to elucidate the juxtaposition between the pancreatic D-cell and A-cell, 1–2 weeks after abdominal vagotomy in chickens. A pancreatic D-cell frequently encircled an A-cell. Around this juxtaposition, several D-cells, characterized by the occurrence of peculiar dense bodies, formed a cluster. Both the D-cell and the A-cell juxtaposed with each other and had an irregularly shaped nucleus with several indentations. Exocytosis of secretory granules from D-cells encircling an A-cell was often observed in the capillary side, but no release of secretory granules from A-cells was detected, except on the capillary side. A few large dense bodies, resembling a multivesicular body, were observed in the A-cell cytoplasm, showed positive acid-phosphatase activity, and contained remnants of several types of cell organelles. They thus seemed to be secondary lysosomes. It is possible that the juxtaposition between the A-cell and the D-cell may be morphological evidence of the inhibitory action on the A-cell by the D-cell. 相似文献
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Salakij C Salakij J Apibal S Narkkong NA Chanhome L Rochanapat N 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2002,31(3):116-126
Background — King cobras (Ophiophagus hannah) have been captive‐bred at Queen Saovabha Memorial Institute since 1996 to supply venom for antivenom production. Hematologic tests would be useful for evaluating the health of the snakes, however, basic hematologic data and morphology have not been described for this species. Objective — The purpose of this study was to determine basic hematologic values and evaluate light microscopic, cytochemical, and electron microscopic characteristics of king cobra blood cells. Methods — Blood samples from 13 wild‐caught and 15 captive‐bred king cobras were collected into EDTA from the ventral caudal vein. A CBC was done using standard methods. Significant differences between groups were determined using t‐tests. Cytochemical stains (periodic acid‐Schiff [PAS], Sudan black B [SBB], a‐naphthyl acetate esterase [ANAE], acid phosphatase [AcP], and p‐glucuronidase [p‐glu]), and scanning and transmission electron microscopy were done using standard techniques. Results — Eighteen snakes (64.3%) were positive for Hepatozoon infection. Hepatozoon organisms were detected nearly twice as frequently in wild‐caught (11/13) as in captive‐bred (7/15) snakes.Total WBC, azurophil, and lymphocyte counts were higher and fib‐rinogen concentration was lower in Hepatozoon‐positive snakes. Captive‐bred snakes had higher RBC values, lower azurophil, het‐erophil and punctate reticulocyte percentages, and higher lymphocyte numbers compared with wild‐caught snakes. Lymphocytes were the most commonly observed WBCs, and stained positive with PAS, ANAE, AcP, and (3–glu. Azurophil granules stained positive with SBB, PAS, and ANAE. Heterophils were the largest WBCs; their granules stained with SBB, ANAE, and (3–glu. Basophil granules stained with PAS, SBB, ANAE, and (3–glu. Thrombocytes were strongly positive with PAS. Transmission electron microscopic examination revealed organelles within all WBCs except eosinophils and revealed the gamonts of Hepatozoon sp in RBCs and azurophils. Conclusions — These results provide comparative hematologic data and a guide for identification of blood cells in wild‐caught and captive‐bred king cobra snakes. Hepatozoon infection was relatively common, but was not associated with severe hematologic abnormalities. 相似文献
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Kreangsak Prihirunkit Chaleow Salakij Suntaree Apibal Nual-Anong Narkkong 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(2):163-168
Hematological, cytochemical and ultrastructural features of blood cells in fishing cat (Felis viverrina) were evaluated using complete blood cell counts with routine and cytochemical blood stains, and scanning and transmission electron microscopy. No statistically significant difference was found in different genders of this animal. Unique features of blood cells in this animal were identified in hematological, cytochemical and ultrastructural studies. This study contributes to broaden hematological resources in wildlife animals and provides a guideline for identification of blood cells in the fishing cat. 相似文献
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Chaleow Salakij Theerapol Sirinarumitr Daraka Tongthainun 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2010,39(2):199-202
Abstract: Hepatozoon gamonts were observed by light microscopy in neutrophils of a male, wild‐caught Leopard Cat. Complete blood counts at presentation and 6 months later were unremarkable. Serologic tests were negative for both FIV and FeLV. A partial sequence of the 18S rRNA gene from the Hepatozoon found in the cat indicated that, compared with all species examined, the protozoan had the closest relationship (99.2% sequence similarity) with the Hepatozoon of the water python (Stegonotus cucullatus). The cat was clinically healthy at last report. Although Hepatozoon has been found in another wild cat in Thailand, this is the first report in a Leopard Cat. The pathogenicity of Hepatozoon in these cats remains uncertain. 相似文献
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