Comparative virulence of Haemophilus parasuis serovars 1 to 7 in guinea pigs.     

V J Rapp-Gabrielson  D A Gabrielson  G J Schamber 《American journal of veterinary research》1992,53(6):987-994

Reference strains for Haemophilus parasuis serovars 1 to 7 were examined for virulence by inoculation of guinea pigs. Guinea pig response to intraperitoneal inoculation was similar for the 7 reference strains. However, apparent differences in virulence were detected after intratracheal inoculation. Cells of the references strains for serovars 1 and 5 were most invasive, causing moribundity or death at higher doses and a persistent septicemia at lower doses. Haemophilus parasuis could be isolated from respiratory and systemic sites; purulent bronchopneumonia, pericarditis, and pleuritis were apparent in infected guinea pigs. Inoculation of cells of the reference strains for serovars 2 and 6 also resulted in bronchopneumonia and moribundity or death in some guinea pigs; however, reisolation of H parasuis and microscopic lesions at necropsy were less pronounced than those observed with serovars 1 and 5. Inoculation of cells of serovars 3, 4 and 7 induced only transient clinical signs and minimal evidence of H parasuis infection at necropsy. The data from intratracheal inoculation of guinea pigs are similar to data from other investigations in swine, indicating differences in the pathogenic potential of H parasuis strains. Thus, guinea pigs may be useful as a laboratory animal model for examining cellular factors associated with virulence and immunogenicity of H parasuis.  相似文献   

Growth response of Ficus benjamina to limited soil volume and soil dilution in a skeletal soil container study     

Felix C.W. Loh  Jason C. Grabosky  Nina L. Bassuk 《Urban Forestry & Urban Greening》2003,2(1):53-62

The interactive effects of rooting volume and nutrient availability in a skeletal soil medium designed to meet street tree and pavement needs were observed in a containerized experiment. Benjamin fig (Ficus benjamina L.) was grown in a stone-soil blended skeletal soil material (CU-Soil™) and compared to a loam soil. The same topsoil used as the soil component in the skeletal soil material was used as the sole component in the comparison soil-only treatment.Plants grown in the skeletal soil material had reduced leaf tissue N content and depressed growth compared with plants grown in non-diluted soil. No other mineral deficiencies were found. Leaf number, chlorophyll concentration, shoot weight, and root characteristics were all affected.Reduced growth from soil dilution could be offset by the provision of an enlarged rooting volume for root development. Large containers of skeletal soil were observed to have smaller root systems compared to equivalent net volumes of loam soil at the first two harvest dates of the study. By the end of the study, the large containers of skeletal soil were observed to have developed larger root systems compared to equivalent net volumes of loam soil; resulting in comparable leaf N levels and total plant dry matter. Plants in skeletal soil had lower shoot: root ratios at the end of the study. Investing resources to further root growth in times of nutrient shortages is a probable plant reaction as evidenced by differences in specific root length between treatments. The study allowed a method for directly partitioning the containerization effect by having equivalent amounts of soil over two volumes.  相似文献   

DNA contents in Texas bluegrass (Poa arachnifera) selected in Texas and Oklahoma determined by flow cytometry     

Jason J. Goldman 《Genetic Resources and Crop Evolution》2015,62(5):643-647

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Nutritional approaches to slow late finishing pig growth: implications on carcass composition and pork quality     

Emma T Helm  Jason W Ross  John F Patience  Steven M Lonergan  Elisabeth Huff-Lonergan  Laura L Greiner  Leah M Reever  Chad W Hastad  Emily K Arkfeld  Nicholas K Gabler 《Journal of animal science》2021,99(1)

Although pork producers typically aim to optimize growth rates, occasionally it is necessary to slow growth, such as when harvest facility capacity is limited. In finishing pigs, numerous dietary strategies can be used to slow growth so pigs are at optimal slaughter body weights when harvest facility capacity and/or access is restored. However, the impact of these diets on pork carcass quality is largely unknown. Thus, this study aimed to evaluate the efficacy of dietary strategies to slow growth in late finishing pigs and evaluate their effects on carcass composition and pork quality. Mixed-sex pigs (n = 897; 125 ± 2 kg BW) were randomly allotted across 48 pens and assigned to 1 of 6 dietary treatments (n = 8 pens/treatment): (1) Control diet representative of a typical finisher diet (CON); (2) diet containing 3% calcium chloride (CaCl₂); (3) diet containing 97% corn and no soybean meal (Corn); (4) diet deficient in isoleucine (LowIle); (5) diet containing 15% neutral detergent fiber (NDF) from soybean hulls (15% NDF); and (6) diet containing 20% NDF from soybean hulls (20% NDF). Over 42 d, pen body weights and feed disappearance were collected. Pigs were harvested in 3 groups (14, 28, and 42 d on feed) and carcass data collected. From the harvest group, 1 loin was collected from 120 randomly selected carcasses (20 loins/treatment) to evaluate pork quality traits. Overall, ADG was reduced in CaCl₂, Corn, and 20% NDF pigs compared with CON pigs (P < 0.001). However, ADFI was only reduced in CaCl₂ and 20% NDF pigs compared with CON (P < 0.001). Feed efficiency was reduced in CaCl₂ and Corn pigs compared with CON (P < 0.001). Hot carcass weights were reduced in CaCl₂ pigs at all harvest dates (P < 0.001) and were reduced in Corn and 20% NDF pigs at days 28 and 42 compared with CON pigs (P < 0.001). In general, CaCl₂ and 20% NDF diets resulted in leaner carcasses, whereas the Corn diet increased backfat by 42 d on test (P < 0.05). Loin pH was reduced and star probe increased in CaCl₂ pigs compared with CON pigs (P < 0.05); no treatments differed from CON pigs regarding drip loss, cook loss, color, firmness, or marbling (P ≥ 0.117). Overall, these data indicate that several dietary strategies can slow finishing pig growth without evidence of behavioral vices. However, changes to carcass composition and quality were also observed, indicating quality should be taken into consideration when choosing diets to slow growth.  相似文献   

Effects of iron injection timing on suckling and subsequent nursery and growing-finishing performance and hematological criteria     

Hayden E Williams  Brittany Carrender  Cierra D Roubicek  Ryan Maurer  Joel M DeRouchey  Jason C Woodworth  Steve S Dritz  Michael D Tokach  Kyle F Coble  Robert D Goodband  Jordan T Gebhardt 《Journal of animal science》2021,99(3)

Two experiments were conducted to evaluate the effects of Fe injection timing after birth on suckling and subsequent nursery and growing-finishing pig performance. The injectable Fe source used in both experiments was GleptoForte (Ceva Animal Health, LLC., Lenexa, KS). GleptoForte contains gleptoferron which is a Fe macromolecule complex. In Exp. 1, a total of 324 newborn pigs (DNA 241 × 600, initially 1.6 ± 0.04 kg body weight [BW]) within 27 litters were used. Two days after birth, all piglets were weighed, and six barrows and six gilts per litter were allotted to 1 of 6 treatments consisting of no Fe injection or 200 mg of injectable Fe provided in a single injection on d 2, 4, 6, 8, or 10 of age. Pigs were weaned (~21 d of age) and allotted to nursery pens with all pigs in each pen having received the same Fe treatment. In Exp. 2, a total of 1,892 newborn pigs (PIC 359 × C40; initially 1.5 ± 0.02 kg BW) within 172 litters were used. One day after birth, piglets were weighed, and 11 pigs within each litter were allotted to 1 of 6 treatments consisting of no Fe injection or 200 mg of injectable Fe provided on d 1, 3, 5, or 7 of age, or 200 mg on d 1 plus 200 mg on d 12 of age. Pigs were weaned (19 d of age) and placed in a commercial wean-to-finish facility in a total of 15 pens with equal representation of treatments in each pen. In both experiments, not providing an Fe injection after birth decreased (P < 0.05) preweaning average daily gain (ADG), weaning weight, and hemoglobin and hematocrit values compared with all other treatments. In Exp. 1, increasing the age that piglets received an Fe injection until 4 or 6 d after birth provided marginal evidence for an improvement (quadratic; P = 0.070) in preweaning ADG. For the nursery period, increasing the age that piglets received an Fe injection improved (quadratic; P = 0.013) d 80 BW, but there was no evidence of a difference (P > 0.10) in d 173 BW at the end of the grow-finish period. In Exp. 2, increasing the age that piglets received a 200 mg Fe injection showed no evidence of difference (P > 0.10) for subsequent nursery and growing-finishing ADG. In both experiments, hemoglobin and hematocrit values were decreased (linear; P < 0.05) at weaning with increasing age when pigs received an Fe injection. These experiments suggest that providing a 200 mg Fe injection within 7 d after farrowing is sufficient for optimizing preweaning and subsequent growth performance.  相似文献   

Diagnostic accuracy of optical coherence tomography for assessing surgical margins of canine soft tissue sarcomas in observers of different specialties     

Josephine A. Dornbusch  Laura E. Selmic  Pin‐Chieh Huang  Jonathan P. Samuelson  Eric M. McLaughlin  Vincent A. Wavreille  Jessica A. Ogden  Brittany Abrams  Alex Kalamaras  Eric Green  Eric T. Hostnik  Lincoln Every  Jason A. Fuerst  Ryan Jennings  Christopher Premanandan  Joshua N. Lorbach  Sarah C. Linn  Aneesh Alex  Janet E. Sorrells  Lingxiao Yang  Stephen A. Boppart 《Veterinary surgery : VS》2021,50(1):111-120

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Comparison of two postoperative complication grading systems after treatment of stifle and shoulder instability in 68 dogs     

Faolain M. Barrett BVM&S  Jason A. Bleedorn DVM MS  DACVS-SA  Kyle D. Hutcheson DVM  DACVS-SA  Bryan T. Torres DVM PhD  DACVS-SA  DACVSMR  Derek B. Fox DVM  PhD  DACVS 《Veterinary surgery : VS》2023,52(1):98-105

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Effects of abamectin on intracellular potassium ion activity and membrane potential in dipteran skeletal muscle     

Elizabeth M. Fitzgerald  Jason Curry  Mustafa B. A. Djamgoz 《Pest management science》1993,37(1):49-55

The effects of abamectin (AVMB₁) on intracellular potassium ion activity (aK_i) and resting membrane potential (E_m) of the skeletal muscle cells of final instar larvae of Phormia terraenovae (Diptera) were investigated using K⁺-selective micro-electrodes. Bathing the preparation in 10^? M AVMB₁(+ 1 ml litre^?1 dimethylsulfoxide) for 60 min caused a significant (31%) decrease in aK_i, whilst E_m depolarized on average by 19 mV (nearly 50% of the original control value). The difference E_K-E_m increased by 9 mV, although E_K (potassium equilibrium potential) remained more negative than E_m. These results could be due to a cationic effect of AVMB₁ possibly involving an increase in K⁺ and Na⁺ conductances of the muscle plasma membrane.  相似文献   

Pneumocystosis associated with canine distemper virus infection in a mink          下载免费PDF全文

Dyer NW  Schamber GJ 《The Canadian veterinary journal. La revue veterinaire canadienne》1999,40(8):577-578

An adult mink from a farm experiencing 100% mortality in affected animals was submitted for diagnostic examination. Clinical history included signs of respiratory disease, oculonasal discharge, and thickening of footpads. Canine distemper virus and Pneumocystis carinii were identified in lung tissue, suggesting immunosuppresion and secondary infection due to morbillivirus disease.  相似文献   

In vitro effects of osteotomy angle and osteotomy reduction on tibial angulation and rotation during the tibial plateau-leveling osteotomy procedure     

Wheeler JL  Cross AR  Gingrich W 《Veterinary surgery : VS》2003,32(4):371-377

OBJECTIVE: To determine the effect of osteotomy angle, reduction technique, and tibial plateau rotation angle on angular and rotational limb deformities. STUDY DESIGN: Geometric comparison using bone models. METHODS: Rotational osteotomies were made in the proximal metaphysis of artificial tibias at 0 degrees, 10 degrees, 20 degrees, -10 degrees, and -20 degrees from perpendicular with respect to either the proximodistal and craniocaudal tibial axes. Negative-numbered angles represented osteotomies made from distal to proximal or caudal to cranial. Changes in tibial angulation and torsion were measured using a 3-dimensional digitizing instrument at tibial plateau rotation angles from 0 degrees to 30 degrees at 5 degrees increments. Two osteotomy reduction techniques were used: complete osteotomy reduction and alignment of the medial cortex. The mean of 5 measurements of torsional and angular tibial deformity for each of the 9 osteotomy orientations in each reduction technique group was obtained. RESULTS: All had increasing angular and rotational deformity as tibial plateau rotation angle increased. In the medially aligned cortex group, all tibias had valgus deformity, and 8 of 9 tibias were internally rotated. In the reduced osteotomy group, minimal angular deformity was seen in tibias with osteotomy variation along the proximodistal axis; however, tibias with osteotomy variation along the craniocaudal axis had angular deformity ranging from 6.0 degrees of varus deformity to 14.3 degrees of valgus deformity. Rotational deformity was affected similarly by osteotomy variation along either axis. Reduction technique had greater affect on angular and rotational deformity than osteotomy angle variation. CLINICAL RELEVANCE: These results suggest that osteotomy reduction may play a greater role in angular and rotational deformity than osteotomy angle, although extreme osteotomy angles should be avoided. To decrease the severity of deformity, we recommend that the osteotomy be made perpendicular to the craniocaudal and proximodistal axes and be completely reduced with less regard for alignment of the medial cortex.  相似文献