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Ahn Huijeong Kim Jeongeun Lee Hansae Lee Eunsong Lee Geun-Shik 《Veterinary research communications》2020,44(2):51-59
Veterinary Research Communications - Inflammasome, a cytosolic multi-protein complex, assembly is a response to sensing intracellular pathogenic and endogenic danger signals followed by caspase-1... 相似文献
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Hydrocarbons and 2-alkylcyclobutanones in sesame seeds ( Sesamum indicum L.) irradiated at 0.5-4 kGy were used to determine the effect of subsequent steaming, roasting, and oil extraction from the roasted samples on the changes in their concentrations. The concentrations of radiation-induced hydrocarbons increased almost linearly (R(2) = 0.8671-0.9953) with the applied dose. The hydrocarbons, 1,7-hexadecadiene and 8-heptadecene, were detected only in the irradiated samples before and after three types of treatments at doses > or =0.5 kGy, but they were not detected in non-irradiated samples before and after treatment. These two hydrocarbons could be used as markers to identify irradiated sesame seeds. The concentrations of the three detected 2-alkylcyclobutanones, 2-dodecylcyclobutanone (2-DCB), 2-tetradecylcyclobutanone (2-TCB), and 2-(5'-tetradecenyl)cyclobutanone (2-TeCB), linearly increased with the irradiation dose. These compounds could be detected at doses > or =0.5 kGy but not in non-irradiated samples. The three types of treatments had no significant effect on the levels of 2-alkylcyclobutanones. 相似文献
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Sesame seeds were irradiated using a (60)Co irradiator (0-4 kGy) and then roasted (220 degrees C for 10 min). To identify the irradiation treatment, physical detection methods like photostimulated luminescence (PSL), thermoluminescence (TL), and electron spin resonance (ESR) have been investigated before and after roasting. The photon counts of the irradiated samples (nonroasted and roasted) were higher than those of nonirradiated ones, making it possible to distinguish the two samples. The threshold values of nonroasted and roasted samples increased linearly with the irradiation dose, respectively. The TL for the nonirradiated nonroasted and roasted samples presented a lower peak at about 300 degrees C, but irradiated samples showed a higher peak at around 150 degrees C. The areas of TL glow curves were 15 times higher in nonroasted as compared with roasted samples. TL ratio [integrated area of TL 1 (the first glow)/TL 2 (the second glow)] obtained by the reirradiation step was 0 in nonirradiated samples and more than 0.15 in irradiated samples. The radiation-induced ESR signals originating from cellulose were determined in irradiated samples before and after roasting. 相似文献
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Huijeong Ahn Jeongeun Kim Sungkyun Kwon Pyeung-Hyeun Kim Hyuk Moo Kwon Eunsong Lee Geun-Shik Lee 《Veterinary research communications》2018,42(4):265-273
Pigs are an important livestock and serve as a large animal model due to physiological and anatomical similarities with humans. Thus, components of the porcine immune system such as inflammasomes need to be characterized for disease control, vaccination, and translational research purposes. Previously, we and others elucidated porcine nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family Pyrin domain containing 3 (NLRP3) inflammasome activation. However, until now, porcine NLR family caspase recruitment domain (CARD)-containing 4 (NLRC4) and absent in melanoma 2 (AIM2) inflammasomes have been not well studied. In this study, we treated well defined NLRC4 and AIM2 inflammasome triggers to porcine peripheral blood mononuclear cells (PBMCs) and murine bone-marrow derived macrophages (BMDMs) and observed interleukin (IL)-1β maturation as a readout of inflammasome activation. NLRC4 (flagellin) and AIM2 (dsDNA) triggers led to IL-1β secretion in both porcine PBMCs and mice macrophages. In addition, porcine and mouse NLRC4 and AIM2 inflammasomes responded differently to NLRP3 inhibitors. Bacterial inflammasome triggers, Salmonella enterica serovar Typhimurium, Listeria monocytogenes, and Escherichia coli, also induced IL-1β secretion in porcine PBMCs. Taken together, we suggest that known triggers of NLRC4 and AIM2 inflammasomes in mice induce IL-1β secretion in porcine PBMCs. 相似文献
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Precision Agriculture - This study proposes a deep learning-based real-time variable flow control system using the segmentation of fruit trees in a pear orchard. The real-time flow rate control,... 相似文献
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