Analysis of gene cassettes of streptomycin-spectinomycin resistance of Hungarian Salmonella enterica serotype Typhimurium strains     

Nógrády N  Gadó I  Pászti J  Király M 《Acta veterinaria Hungarica》2003,51(2):137-151

By PCR using the ant(3")-Ia primer pair the aadA gene was detected in 34 streptomycin- and spectinomycin-resistant Salmonella enterica serotype Typhimurium strains. Out of them 12 belonged to DT104 and 22 to non-DT104 phage type. Using different primer combinations it was demonstrated that this gene was integron-associated in all cases: in the DT104 strains it was generally contained by a 1 kb integron while in the majority of the non-DT104 strains by a 2.05 kb (less often by a 1.9 or 1 kb) integron. In the case of integrons carrying multiple cassettes the cassette containing the aadA gene was located closer to the 3' end of the integron. The aadA genes of DT104 and non-DT104 strains were different: in the former group the aadA2 gene, while in the latter group (constituted by strains of five different phages types as well as unclassifiable and untypable strains) the aadA1 gene could be identified. The RH50/RH51 primer pair described by Collis and Hall (1992) proved to be suitable for rapid discrimination between the aadA1 and aadA2 genes on the basis that the RH51 primer bound exclusively to the aadA2 gene.  相似文献   

Cell-free extract from porcine induced pluripotent stem cells can affect porcine somatic cell nuclear reprogramming     

Jin-Gu NO  Mi-Kyung CHOI  Dae-Jin KWON  Jae Gyu YOO  Byoung-Chul YANG  Jin-Ki PARK  Dong-Hoon KIM 《The Journal of reproduction and development》2015,61(2):90-98

Pretreatment of somatic cells with undifferentiated cell extracts, such as embryonic stem cells and mammalian oocytes, is an attractive alternative method for reprogramming control. The properties of induced pluripotent stem cells (iPSCs) are similar to those of embryonic stem cells; however, no studies have reported somatic cell nuclear reprogramming using iPSC extracts. Therefore, this study aimed to evaluate the effects of porcine iPSC extracts treatment on porcine ear fibroblasts and early development of porcine cloned embryos produced from porcine ear skin fibroblasts pretreated with the porcine iPSC extracts. The Chariot^TM reagent system was used to deliver the iPSC extracts into cultured porcine ear skin fibroblasts. The iPSC extracts-treated cells (iPSC-treated cells) were cultured for 3 days and used for analyzing histone modification and somatic cell nuclear transfer. Compared to the results for nontreated cells, the trimethylation status of histone H3 lysine residue 9 (H3K9) in the iPSC-treated cells significantly decreased. The expression of Jmjd2b, the H3K9 trimethylation-specific demethylase gene, significantly increased in the iPSC-treated cells; conversely, the expression of the proapoptotic genes, Bax and p53, significantly decreased. When the iPSC-treated cells were transferred into enucleated porcine oocytes, no differences were observed in blastocyst development and total cell number in blastocysts compared with the results for control cells. However, H3K9 trimethylation of pronuclear-stage-cloned embryos significantly decreased in the iPSC-treated cells. Additionally, Bax and p53 gene expression in the blastocysts was significantly lower in iPSC-treated cells than in control cells. To our knowledge, this study is the first to show that an extracts of porcine iPSCs can affect histone modification and gene expression in porcine ear skin fibroblasts and cloned embryos.  相似文献   

Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny   总被引：2，自引：1，他引：2  

A. Gawlicka  S. J. Teh  S. S. O. Hung  D. E. Hinton  J. de la Noüe 《Fish physiology and biochemistry》1995,14(5):357-371

Ontogenetic changes in digestive tract histology and digestive enzyme histochemistry were investigated 11 to 36 days post-hatch in white sturgeon Acipenser transmontanus larvae. From initiation of exogenous feeding (12 days post-hatch), larvae were fed a commercial salmonid diet for the ensuing 24 days. The digestive system of white sturgeon displayed a high degree of morphologic organization and functionality at the onset of exogenous feeding. An enhancement of digestive capacities occurred with transition to active feeding. On day 2 of feeding, there was a clear increase of alkaline phosphatase, aminopeptidase M, dipeptidyl peptidase IV, and -glutamyl transpeptidase activity in the brush border of the spiral intestine. This strong activity is an apparent confirmation of the importance of this segment of the intestine for protein digestion and nutrient absorption. The functional development of the pyloric intestine occurred on day 4 and was concomitant with an increase in the activity of brush border and cytoplasmic enzymes such as acetylcholinesterase, dipeptidyl peptidase II, - and -galactosidases. The absence of acetylcholinesterase, lactase, nonspecific esterase, and weak activity of exopeptidases and alkaline phosphatase in the anterior intestine suggests that this segment of the intestine may be less important in nutrient absorption than the pyloric and spiral intestines. The observed quantitative and qualitative differences in enzyme activity along the intestine indicate a high degree of specialization of each segment for specific digestive and absorptive processes.  相似文献   

Growth of Daphnia magna on urban wastewaters tertiarily treated with Scenedesmus sp.     

D. Proulx  J. de la Noüe 《Aquacultural Engineering》1985,4(2):93-111

The productivity and composition of Daphnia magna were measured for high density populations grown in 5-litre flow-through tanks supplied with a tertiary effluent. This effluent was urban secondary wastewaters biologically treated with intensive cultures of a microalga Scenedesmus sp.Three daily feeding rates (575, 1150, 1750 mg dry weight algae day^?1 tank^?1), obtained by adjusting the flow of a tertiary effluent containing 115 mg dry weight of algae litre^?1, allowed average productions of 0·9, 1·6 and 2·6 g of Daphnia (wet basis) litre^?1 week^?1 which were proportional to the rations. When the flow of tertiary effluent was increased to 40 litres day^?1 tank^?1 (ad libitum), the average yield rose to 3·6 g of daphnids (wet basis) litre^?1 week^?1. The maximum population reached 48 000 individuals litre^?1. The daphnid/algae evergy conversion ratio was 0·4. Algae were removed by Daphnia at a maximum efficiency of 95%. The composition of Daphnia biomasses was 59·5% protein, 9·5% fat and 5·6% carbohydrate on a dry weight basis. No deficiencies in methionine and linolenic acid were noted.Results indicated the remarkable potential of urban wastewaters for the production of protein and the high productivity of Daphnia magna.  相似文献   

关于原山、蒙山国有林场改革的实践与思考——中共国家林业局党校第44期党员领导干部进修班赴临沂和淄博教学考察调研报告     

Class No.  Party School of SAT 《国家林业局管理干部学院学报》2014,13(4)

在国有林场改革不断推进的大背景下,各级政府探索破解国有林场生存和发展难题,摸索出从维护生态效益和社会效益出发,逐步将国有林场纳入财政全额拨款的公益性事业单位和社会保障体系等措施,取得了一些成功的经验.同时,各国有林场也结合自身特点,发挥地域、资源和生态优势,在改革发展中取得了一定成绩.原山国有林场和蒙山国有林场的改革既有相同点又有差异.创新发展理念,实现可持续发展;放活林场经营自主权,激发经营主体的主动性与积极性;创新经营机制,增强林场发展活力;充分发挥林业服务功能,提高国有林场的社会地位等经验值得借鉴.  相似文献   

滴灌成套设备和低压大流量滴头研制与应用     

岳兵 《节水灌溉》1992,(1)

经过10多年的科学研究,我国在微灌设备研制方面取得了一定进展,先后生产试制了微灌用聚乙烯管材与管件,研制出了一批灌水器和过滤、施肥装置,促进了微灌技术的推广运用。对微管灌水器进行的系列化研究,降低了工程造价,提高了灌水质量。  相似文献   

A NOTE ON THE SURFACE VISCOSITY OF COLLOIDAL SOLUTIONS     

Du Noöo PL 《Science (New York, N.Y.)》1925,61(1570):117-118

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IMMUNOLOGICAL REACTIONS AND VISCOSITY     

du Noüy L 《Science (New York, N.Y.)》1935,82(2124):254-256

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THE SURFACE EQUILIBRIUM OF COLLOIDAL SOLUTIONS. II     

Du Noüy PL 《Science (New York, N.Y.)》1924,60(1554):337-338

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A simple and efficient method for isolating small RNAs from different plant species     

FlordeFátima Rosas-Cárdenas  Noé Durán-Figueroa  Jean-Philippe Vielle-Calzada  Andrés Cruz-Hernández  Nayelli Marsch-Martínez  Stefan de Folter 《Plant methods》2011,7(1):4

Background  

Small RNAs emerged over the last decade as key regulators in diverse biological processes in eukaryotic organisms. To identify and study small RNAs, good and efficient protocols are necessary to isolate them, which sometimes may be challenging due to the composition of specific tissues of certain plant species. Here we describe a simple and efficient method to isolate small RNAs from different plant species.  相似文献