Suppression of ovarian progesterone production in dairy cows using an implant of GnRH-agonist (deslorelin) for the purpose of evaluating progesterone metabolism     

AR RABIEE  KL MACMILLAN  F SCHWARZENBERGER  D THALLER  MJ RATHBONE  TE TRIGG 《Australian veterinary journal》2001,79(10):690-694

OBJECTIVE: To evaluate the potential of an implant of a GnRH-agonist (deslorelin) to create a progesterone free animal suitable for studying progesterone (P4) metabolism in intact cows by measuring blood P4 and faecal P4 metabolites. METHODS: Experiment 1: Eighteen non-lactating cycling Holstein-Friesian cows, 4 to 7 years old, were allocated to one of three groups to study plasma P4 concentrations preceding an intravaginal insert. These groups comprised: i) a deslorelin group (GnRH-agonist implanted); ii) a PGF group receiving two injections of prostaglandin (PGF2alpha) 12 days apart; and, iii) an ovariectomised (OVX) group. An intravaginal device (CIDR) was inserted into the vagina of each animal and left in place for 11 days. Plasma P4 concentrations were measured during the study period. Experiment 2: Twelve non-lactating cycling Holstein-Friesian cows, 4 to 7 years old, were allocated to two groups: i) a deslorelin group (GnRH-agonist implanted); and ii) an ovariectomised group. Plasma P4 and faecal P4 metabolites (20-oxo-pregnanes, 20alpha-OH and 20beta-OH) were monitored for a period of 5 weeks. RESULTS: Experiment 1: Average plasma P4 concentration did not differ between the three groups (1.28, 1.43 and 1.55 ng/mL for deslorelin, OVX and PGF cows, respectively, P = 0.8) during the period of supplementation. Experiment 2: There was no difference in plasma P4 (mean plasma P4 < 0.02 ng/mL, P = 0.9) and faecal P4 metabolites between deslorelin and OVX cows 2 weeks after the implantation (P = 0.7). CONCLUSIONS: These data showed that a GnRH-agonist (deslorelin) implant may be used as an alternative to ovariectomy to create a progesterone free animal suitable for studying the metabolism of administered P4.  相似文献   

The next step — a monthly journal     

William E. Jones DVM  PhD 《Journal of Equine Veterinary Science》1992,12(6):329

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Identification of the surface components of Trypanosoma evansi.     

U E Uche  C A Ross  T W Jones 《Research in veterinary science》1992,53(2):252-253

125I-labelling was used to characterise the surface components of five stocks of Trypanosoma evansi. Two components of 67 and 60.5 kD were labelled in two of the stocks, a single 60.5 kD component in two other stocks and no components in the remaining stock. These differences are probably related to the labelling method and biochemical differences between the stocks.  相似文献   

Increase in Salmonella enterica subspecies diarizonae serovar 61:k:1,5,(7) in sheep.     

R H Davies  S J Evans  B E Preece  S Chappell  S Kidd  Y E Jones 《The Veterinary record》2001,149(18):555-557

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Response time of broiler chickens to cimaterol: meat tenderness, muscle composition fiber size, and carcass characteristics.   总被引：4，自引：0，他引：4  

B L Gwartney  S J Jones  C R Calkins 《Journal of animal science》1992,70(7):2144-2150

The response time to cimaterol (CIM), a beta-adrenergic agonist, by broiler chickens for carcass characteristics, muscle composition, muscle fiber size, catheptic enzyme activity, and tenderness was determined. Two trials were conducted in which chickens were fed a control diet (CON) containing 0 ppm of CIM or a diet containing 1 ppm of CIM. Trial 1 consisted of 55, 31-d-old broiler chickens individually fed for up to 48 h. At 0, 6, 12, 18, 24, and 48 h, five CON and five CIM-fed chickens were killed. Trial 2 consisted of 160, 33-d-old broiler chickens group-fed for up to 14 d. At 2, 4, 6, 8, 10, 12, and 14 d, 10 CON and 10 CIM-fed chickens were killed. The breast muscle (BM) and leg muscle (LM) weight, cathepsin B and L activities, DNA, RNA, and protein concentration, and BM shear force value (SFV) were measured in both trials. Thigh muscle (TM) SFV were measured in Trial 2 only. Fiber size of BM was measured (five birds per treatment) at d 2, 6, 10, and 14. In Trial 1, BM weight and SFV were lower in CIM-fed birds at 6 h (P less than .05). In Trial 2 BM SFV were higher at d 8 (P = .06) and d 10 (P less than .05) in CIM-fed chickens. The SFV of CIM-fed chickens were higher at d 4, 8, 10, 12, and 14 (P less than .05).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Muscle relaxants in canine anaesthesia 2: Clinical application     

R. S. Jones 《The Journal of small animal practice》1992,33(9):423-429

There are a variety of factors which are likely to influence the action of muscle relaxants in canine anaesthesia. These include age, body temperature and muscle diseases. Of the anaesthetic agents it is only the inhalational anaesthetic agents which significantly increase the duration of action of muscle relaxants. Antibiotic therapy particularly with the aminoglycoside antibiotics is likely to increase their duration of action. The indications for the use of muscle relaxants and the main contraindications such as the absence of anaesthetic equipment and the inability to ensure unconsciousness are discussed. The choice of anaesthetic technique together with a discussion on the premedication induction and maintenance of anaesthesia are important factors when using relaxants as is the technique of artificial ventilation. The various advantages and disadvantages of neuromuscular block monitoring are discussed as is the reversal of neuromuscular block.  相似文献   

Characterization of Brucella canis protein antigens and polypeptide antibody responses of infected dogs   总被引：1，自引：0，他引：1  

L E Carmichael  J C Joubert  L Jones 《Veterinary microbiology》1989,19(4):373-387

The cytoplasmic protein antigens (CPAg) of Brucella canis were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and analysis of 35S-labeled polypeptides. Approximate molecular weights of the immunoreactive polypeptides were determined by migration patterns of the immunoprecipitated polypeptides after SDS-PAGE or Western immunoblotting of sera collected at various times after experimental infection of dogs. Polypeptides were specifically precipitated by sera of infected dogs, but not from the sera of normal or false-positive (seropositive, non-infected) animals. During the initial month after infection, proteins with molecular weight masses (MW) of approximately 18, 22, 31, 42 and 54 kDa were commonly recognized. A 20-kDa polypeptide was first recognized at 8-10 weeks after infection, but it was detected inconsistently after 6 months. Additional polypeptides detected from 2 to 12 months post-infection had MW of 22, 66-68 and, less regularly, 42, 60, 82, 100 and greater than 200 kDa. The polypeptides most consistently recognized in sera from B. canis-infected dogs had MW of 18, 22 and 68 kDa.  相似文献   

PRELIMINARY FINDINGS FOLLOWING THE USE OF A CONTINUOUS INFUSION OF XYLAZINE,GGE AND KETAMINE TO MAINTAIN GENERAL ANAESTHESIA IN 40 HORSES UNDERGOING A WIDE VARIETY OF SURGICAL PROCEDURES     

L.E. Young  D.H. Bartram  M. Diamond  A. Gregg  R.S. Jones 《Veterinary anaesthesia and analgesia》1991,18(Z1):171-174

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Ocular pathology of caprine beta-mannosidosis     

J A Render  K L Lovell  M Z Jones  C A Wheeler 《Veterinary pathology》1989,26(5):444-446

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What is your diagnosis? Mixed echoic parenchymal pattern and the presence of target lesions consistent with neoplastic masses     

N E Love  C Jones 《Journal of the American Veterinary Medical Association》1989,195(9):1285-1286

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