全文获取类型
收费全文 | 316篇 |
免费 | 45篇 |
国内免费 | 1篇 |
专业分类
林业 | 32篇 |
农学 | 2篇 |
基础科学 | 1篇 |
53篇 | |
综合类 | 28篇 |
农作物 | 15篇 |
水产渔业 | 32篇 |
畜牧兽医 | 167篇 |
园艺 | 9篇 |
植物保护 | 23篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 6篇 |
2021年 | 23篇 |
2020年 | 17篇 |
2019年 | 21篇 |
2018年 | 12篇 |
2017年 | 25篇 |
2016年 | 15篇 |
2015年 | 10篇 |
2014年 | 14篇 |
2013年 | 14篇 |
2012年 | 24篇 |
2011年 | 28篇 |
2010年 | 18篇 |
2009年 | 14篇 |
2008年 | 22篇 |
2007年 | 17篇 |
2006年 | 17篇 |
2005年 | 9篇 |
2004年 | 17篇 |
2003年 | 10篇 |
2002年 | 3篇 |
2001年 | 2篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1974年 | 1篇 |
1902年 | 1篇 |
1900年 | 1篇 |
1883年 | 1篇 |
排序方式: 共有362条查询结果,搜索用时 15 毫秒
1.
2.
3.
Anna Bassols José Angel Robles-Guirado Laura Arroyo Lourdes Soler Natalia García Raquel Pato Raquel Peña Yolanda Saco Ramon Armengol Fermín Lampreave María A. Alava Francesca Canalias Matilde Piñeiro 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2023,52(Z1):64-74
4.
Gutiérrez SE Dolcini GL Arroyo GH Rodriguez Dubra C Ferrer JF Esteban EN 《American journal of veterinary research》2001,62(10):1571-1577
OBJECTIVE: To develop a blocking ELISA for detection of bovine leukemia virus (BLV) antibodies that is comparable to a radioimmunoprecipitation (RIP) assay, to evaluate use of this ELISA for identification of BLV-infected herds, and to develop a polymerase chain reaction (PCR) assay for direct diagnosis of infection with BLV. SAMPLE POPULATION: Serum samples and pooled bulk-tank milk samples from cattle. PROCEDURE: The blocking ELISA was developed, using BLV gp51 as antigen, captured by a selected bovine polyclonal serum. A nested PCR was conducted with primers specific for a segment of the pol region of the BLV genome. RESULTS: Sensitivity and specificity of the ELISA were comparable to those of the RIP assay. Use of the ELISA on pooled milk samples allowed identification of herds in which prevalence of BLV infection among lactating cows was as low as 2.5%. Pooled milk samples from BLV-free herds did not react in the ELISA. All cattle that had positive results for the nested PCR had BLV antibodies, but cattle with consistantly low antibody titers required examination of sequential DNA samples to detect viral sequences. None of the 63 antibody-negative cattle had positive results for the PCR. CONCLUSIONS AND CLINICAL RELEVANCE: This ELISA is a highly specific and sensitive assay for the detection of BLV antibodies in serum and milk samples of cattle. Examination of pooled milk samples with the ELISA provides a reliable, practical, and economic procedure for identification of BLV-infected herds. The nested PCR also constitutes a specific procedure for direct diagnosis of infection with BLV. 相似文献
5.
6.
7.
Sophie Labrut Monique Wyers Julien Miclard Marie-Anne Colle 《Journal of zoo and wildlife medicine》2007,38(2):323-328
A 15-year-old female harbor seal (Phoca vitulina) was referred to the Nantes Veterinary School, Nantes, France, with a clinical history of anorexia, seizures, and left hemiplegia. Cerebrospinal fluid analysis revealed large numbers of neoplastic lymphoid cells. After injection of a contrast agent into the cerebrospinal space, radiographs demonstrated an asymmetry of the right lateral ventricle. Necropsy examination revealed a marked edema of the right frontal lobe, extending to the basal nuclei and thalamus in the brain. Histological examination of the brain revealed leptomeningeal lymphoma. Immunohistochemical labeling demonstrated that the neoplasm was of T-cell origin. No significant macroscopic or microscopic lesions were observed in the other organs examined, including lymphoid organs. This is the first report of primary leptomeningeal lymphoma in a harbor seal. 相似文献
8.
Julien Ruelle Jacques Beauchene Anne Thibaut Bernard Thibaut 《Annals of Forest Science》2007,64(5):503-510
On 10 trees from 10 species of French Guyana tropical rainforest in a clear active process of restoring verticality growth strains were measured in situ in order to determine the occurrence of tension wood within samples. Wood specimens were cut in the vicinity of the growth strains measurements in order to measure some mechanical and physical properties. As suspected, tensile growth strains was very much higher in tension wood zone, because longitudinal modulus of elasticity was slightly higher. Longitudinal shrinkage was also much higher in tension wood than in opposite wood. 相似文献
9.
10.
Serge MORAND Frédéric BORDES Hsuan‐Wien CHEN Julien CLAUDE Jean‐François COSSON Maxime GALAN Gábor Á CZIRJÁK Alex D GREENWOOD Alice LATINNE Johan MICHAUX Alexis RIBAS 《Integrative zoology》2015,10(5):409-423
We summarize the current knowledge on parasitism‐related invasion processes of the globally invasive Rattus lineages, originating from Asia, and how these invasions have impacted the local epidemiology of rodent‐borne diseases. Parasites play an important role in the invasion processes and successes of their hosts through multiple biological mechanisms such as “parasite release,” “immunocompetence advantage,” “biotic resistance” and “novel weapon.” Parasites may also greatly increase the impact of invasions by spillover of parasites and other pathogens, introduced with invasive hosts, into new hosts, potentially leading to novel emerging diseases. Another potential impact is the ability of the invader to amplify local parasites by spillback. In both cases, local fauna and humans may be exposed to new health risks, which may decrease biodiversity and potentially cause increases in human morbidity and mortality. Here we review the current knowledge on these processes and propose some research priorities. 相似文献