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Products of unfavorable chemical equilibria are not readily observed because their high energy and increased reactivity result in low concentrations. Biological macromolecules use binding forces to access unfavorable equilibria and stabilize reactive intermediates by isolating them from the medium. In a similar vein, we describe here a synthetic receptor that allows direct observation of labile tetrahedral intermediates: hemiaminals formed in the reaction of an aldehyde carbonyl group with amines. The receptor encapsulates alkyl-substituted primary amines, then orients them toward a covalently tethered aldehyde function. The hemiaminal intermediates appear at high concentration, confined from the bulk solution and observable at ambient temperature by conventional nuclear magnetic resonance spectroscopy. 相似文献
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Hunter J. Piegols Brittany E. Abrams Janis M. Lapsley Megan T. Cray Josephine A. Dornbusch Christina Murphy Brandan G. Wustefeld-Janssens Carlos H. Souza Marine Traverson Pierre Amsellem Elroy Williams Owen T. Skinner Julius M. Liptak Julie A. Stephens Laura E. Selmic 《Veterinary and comparative oncology》2023,21(4):673-684
Adrenalectomies for canine adrenal tumours are associated with peri-operative morbidity and mortality. Objectives of this study included assessing the prognostic value of tumour- or surgery-related variables in predicting peri-operative mortality and overall survival in dogs undergoing adrenalectomies for primary adrenal tumours as well as pre-treatment with phenoxybenzamine on survival to discharge with pheochromocytomas specifically. A multi-institutional retrospective cohort study was performed across nine institutions. Electronic medical record searches identified 302 dogs which met the inclusion criteria. Data collected included dog-related, tumour-related, treatment-related, surgery-related, and outcome variables. Univariate and multivariable logistic regression and cox proportional hazards models were used to identify variables associated with death prior to discharge and tumour-related survival. Overall, 87% of dogs survived to discharge with a tumour-related survival time of 3.96 years. Post-operative complications were reported in 25%. Increased surgical time (p = 0.002) and pre-surgical medical treatment other than phenoxybenzamine (p = 0.024) were significantly associated with increased peri-operative mortality while ureteronephrectomy (p = 0.021), post-operative pancreatitis (p = 0.025), and post-operative aspiration pneumonia (p < 0.001) were significantly associated with decreased overall survival. Phenoxybenzamine pretreatment had no effect on peri-operative mortality. Thirty-seven of 45 (82%) dogs with pheochromocytomas not pretreated survived to discharge, and 50 of 59 (85%) dogs with pheochromocytomas pretreated with phenoxybenzamine survived to discharge (p = 0.730). This study provides information on risk factors for death prior to discharge and tumour-related survival that may help guide clinical management and owner expectations. In addition, the study findings challenge the previously reported benefit of phenoxybenzamine for pretreatment of dogs undergoing adrenalectomies for pheochromocytomas. 相似文献
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Sap flow meters based on the stem heat balance method were used to measure the mass flow rates or water use in young potted tea (Camellia sinensis L.) plants of clones AHP S15/10 and BBK35. The meters were constructed on site and installed onto the stem or branch sections of field growing plants in an experiment originally designed to study the effects of plant population density and drought on the productivity and water use of young tea clones. The objective of the study was to use the SHB method as a first attempt to use sap flow meters for determining the water use of young tea growing in the field under well watered conditions in Tanzania. The results are reported and recommendation made for further work on using the technique. 相似文献
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D. Julius 《Forstwissenschaftliches Centralblatt》1934,56(4):134-138
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