Effect of fast protein liquid chromatography fractionated salivary gland extracts from different ixodid tick species on interleukin-8 binding to its cell receptors   总被引：2，自引：0，他引：2  

Kocáková P  Sláviková M  Hajnická V  Slovák M  Gasperík J  Vancová I  Fuchsberger N  Nuttall PA 《Folia parasitologica》2003,50(1):79-84

Interleukin-8 plays a critical role in inflammatory processes. Hence generation of molecules with anti-IL-8 activity is likely to be important for successful feeding and for survival of the ticks. Anti-IL-8 activity was studied in saliva of three ixodid tick species--Dermacentor reticulatus (Fabricius, 1794), Rhipicephalus appendiculatus Neumann, 1901, and Amblyomma variegatum (Fabricius, 1794). The greatest activity was shown in saliva prepared from D. reticulatus. The activity was attributed to tick salivary gland molecules that bind to IL-8, preventing binding of the chemokine to its specific receptor, rather than to occupation of the IL-8 cell receptor by the tick molecules. The distribution of anti-IL-8 activity in fast protein liquid chromatography (FPLC) fractions of salivary gland extracts (SGE) derived from adult female D. reticulatus, R. appendiculatus and A. variegatum was compared directly by both ELISA and receptor-binding inhibition assays. The correspondence in results with fractions of SGE from ELISA is consistent with detection of tick molecules that inhibit IL-8 binding to its receptor. As IL-8 is an important chemoattractant and activator of neutrophils, the presence of an anti-IL-8 activity in tick saliva indicates that neutrophils play an important role in the host response to parasitism by ticks.  相似文献   

Stress exposure during the preimplantation period affects blastocyst lineages and offspring development     

Ján BURKU?  Martina KA?MAROVá  Janka KUBANDOVá  Natália KOKO?OVá  Kamila FABIANOVá  Du?an FABIAN  Juraj KOPPEL  ?tefan ?IKO? 《The Journal of reproduction and development》2015,61(4):325-331

We found retardation of preimplantation embryo growth after exposure to maternal restraint stress during the preimplantation period in our previous study. In the present study, we evaluated the impact of preimplantation maternal restraint stress on the distribution of inner cell mass (ICM) and trophectoderm (TE) cells in mouse blastocysts, and its possible effect on physiological development of offspring. We exposed spontaneously ovulating female mice to restraint stress for 30 min three times a day during the preimplantation period, and this treatment caused a significant increase in blood serum corticosterone concentration. Microscopic evaluation of embryos showed that restraint stress significantly decreased cell counts per blastocyst. Comparing the effect of restraint stress on the two blastocyst cell lineages, we found that the reduction in TE cells was more substantial than the reduction in ICM cells, which resulted in an increased ICM/TE ratio in blastocysts isolated from stressed dams compared with controls. Restraint stress reduced the number of implantation sites in uteri, significantly delayed eye opening in delivered mice, and altered their behavior in terms of two parameters (scratching on the base of an open field test apparatus, time spent in central zone) as well. Moreover, prenatally stressed offspring had significantly lower body weights and in 5-week old females delivered from stressed dams, fat deposits were significantly lower. Our results indicate that exposure to stress during very early pregnancy can have a negative impact on embryonic development with consequences reaching into postnatal life.  相似文献   

Factors affecting the persistence of traditional agricultural landscapes in Slovakia during the collectivization of agriculture     

Juraj Lieskovský  Pavol Kenderessy  Jana Špulerová  Tibor Lieskovský  Peter Koleda  Felix Kienast  Urs Gimmi 《Landscape Ecology》2014,29(5):867-877

Collectivization of agriculture (1950s–1970s) was one of the most important periods in landscape development in Slovakia. Traditionally managed agricultural landscapes, that covered more than half of the Slovak territory, were transformed into large-scale fields and only fragments of traditional agricultural landscapes survived. We mapped the remaining traditional agricultural landscapes using aerial photos and historical maps. We then statistically analyzed the various geographical factors and their influence on the transformation process of traditional and collectivized fields, i.e., slope steepness, soil fertility, distance from settlements and isolation from regional capital cities. The comparison was performed using classification tree analysis. We constructed a set of decision rules that explain why fields were managed traditionally or collectivized. Our findings show that traditional agricultural fields were more likely to persist on steep terrain, less fertile soils, and on locations that were closer to the settlements, but more isolated from the regional capital cities. Steepness played the most important role: small-scale fields located on steep areas were not accessible to heavy machinery and therefore, frequently survived the collectivization. We show that the selected geographical factors are good explanatory variables for the collectivization of arable fields and orchards. For vineyards and grasslands, however, the explanatory power of the selected geographical factors is lower, and we suspect that other factors, not depicted in the analysis play an important role.  相似文献   

Impacts of population growth, economic development, and technical change on global food production and consumption   总被引：3，自引：0，他引：3  

Uwe A. Schneider  Petr HavlíkErwin Schmid  Hugo ValinAline Mosnier  Michael ObersteinerHannes Böttcher  Rastislav SkalskýJuraj Balkovi?  Timm SauerSteffen Fritz 《Agricultural Systems》2011,104(2):204-215

Over the next decades mankind will demand more food from fewer land and water resources. This study quantifies the food production impacts of four alternative development scenarios from the Millennium Ecosystem Assessment and the Special Report on Emission Scenarios. Partially and jointly considered are land and water supply impacts from population growth, and technical change, as well as forest and agricultural commodity demand shifts from population growth and economic development. The income impacts on food demand are computed with dynamic elasticities. Simulations with a global, partial equilibrium model of the agricultural and forest sectors show that per capita food levels increase in all examined development scenarios with minor impacts on food prices. Global agricultural land increases by up to 14% between 2010 and 2030. Deforestation restrictions strongly impact the price of land and water resources but have little consequences for the global level of food production and food prices. While projected income changes have the highest partial impact on per capita food consumption levels, population growth leads to the highest increase in total food production. The impact of technical change is amplified or mitigated by adaptations of land management intensities.  相似文献   

The influence of sex on biochemical markers of bone turnover in dogs     

Belić M  Kušec V  Svetina A  Grizelj J  Robić M  Vrbanac Z  Benić M  Turk R 《Research in veterinary science》2012,93(2):918-920

Biochemical markers of bone turnover have been shown to be useful as inexpensive and noninvasive tools for monitoring skeletal health. The reference range for bone markers in dogs has been set by different age groups. However, other sources of biological variations were not fully investigated in dogs. To explore whether sex influences the interpretation of bone marker data we examined serum bone markers in 33 male and 25 female dogs. The bone markers selected for this study were: bone alkaline phosphatase (BALP) and osteocalcin (OC) as indicators of bone formation, and C-terminal telopeptide (CTx) of type I collagen as marker of bone resorption. All concentrations of bone markers were lower, but still within the reference range reported for dogs. We found statistically significant differences of the median OC and CTx serum concentrations between males and females. The results of this study suggest that there are sex differences in biochemical markers of bone turnover in dogs which should be considered in interpretation of bone marker data.  相似文献   

Leukocytic responses and intestinal mucin dynamics of broilers protected with Enterococcus faecium EF55 and challenged with Salmonella Enteritidis     

Martin Levkut  Viera Revajová  Andrea Lauková  Zuzana Ševčíková  Viera Spišáková  Zita Faixová  Mária Levkutová  Viola Strompfová  Juraj Pistl  Mikuláš Levkut 《Research in veterinary science》2012,93(1):195-201

The protective effect of Enterococcus faecium EF55 in chickens challenged with Salmonella enterica serovar Enteritidis phage type 4 (SE PT4) was assessed. The antibacterial effect on the bacterial microflora in the small intestine in relation to white blood cell count, phenotyping of peripheral blood and intestinal lymphocytes, functional activity of lymphocytes and phagocytes and mucin quantitation were investigated. Day-old chicks (85) were randomly divided into four groups. The probiotic group (EF) and Salmonella + probiotic group (EFSE) received E. faecium EF55 (10⁹ CFU – 3 g/group/day) for 21 days. The Salmonella group (SE) and EFSE group were infected with Salmonella Enteritidis (10⁸ CFU in 0.2 ml PBS) in a single dose per os on day four of the experiment. The control group chicks (C) were fed a commercial diet without added bacteria. Supplementation of EF55 in the diet of the chickens in the EFSE group, challenged with S. Enteritidis, caused the density of the intestinal mucin layer to increase significantly in non-specific regions (duodenum and jejunum), but decrease significantly in target regions (caeca) for S. Enteritidis. Probiotic treatment also appeared to result in a significantly higher number of lymphocytes in peripheral blood and a tendency to increase CD3, CD4, CD8, and IgM positive cells 3 days post-infection with S. Enteritidis. The results demonstrated an antibacterial effect and suggested that EF55 had a moderating effect on intestinal mucin production and leukocytic response in the early phase of S. Enteritidis infection.  相似文献   

Contamination history of suspended river sediments accumulated in oxbow lakes over the last 25 years   总被引：3，自引：2，他引：1  

Ondrej Bábek  Klára Hilscherová  Slavomír Nehyba  Josef Zeman  Martin Famera  Juraj Francu  Ivan Holoubek  Jirí Machát  Jana Klánová 《Journal of Soils and Sediments》2008,8(3):165-176

Background, aims, and scope  Embankment of meandering river systems in many industrial areas results in the formation of artificial oxbow lakes that may act as perennial or intermittent traps for river sediments. Their deposits can be dated using a combination of historical and stratigraphic data, providing a good means to study historical records of contamination transported by rivers. Contamination history over the last few decades is of special significance for Central and Eastern Europe as it can reflect high pollutant levels in the second half of the twentieth century and the subsequent improvement after the fall of the Iron Curtain. The purpose of this study was to investigate recent sediments of an oxbow lake of the Morava River, Czech Republic, their stratigraphic records, sediment architecture, and history of contamination. Materials and methods  Seven ground-penetrating radar (GPR) profiles and three sediment cores up to 4 m deep were studied. The stratigraphy of the cores was inferred from visible-light spectrophotometry, X-ray radiography, grain size analysis, and semiquantitative modal analysis of sandy fractions. The sediments were dated using the ¹³⁷Cs mass activity and combinations of stratigraphic and historical data. The cores were sampled for concentrations of heavy metals and persistent organic pollutants. Wet sampled, lyophilized, and sieved sediments were extracted and analyzed for heavy metals by inductively coupled plasma mass spectrometry (ICP-MS) of aqua regia leachate and for persistent organic pollutants by gas chromatography (GC-ECD and GC-MS). Results  Three distinct sedimentary sequences (S1, S2, and S3) were identified. The basal sequence S1 represents river channel sediments deposited before the formation of the oxbow lake, most likely before the 1930s. The boundary between the S1 and S2 sequence correlates with the level of sediment dredging from 1981 evidenced from historical data. The overlying sequences S2 and S3 represent a postdredging sediment wedge, which progrades into the lake. ¹³⁷Cs dating revealed a distinct Chernobyl 1986 peak at ∼150 cm depth inferring sedimentation rates up to 7.7 cm/year. Sediment contamination abruptly increased from the pre-1930s deposits to the post-1981 deposits. The concentration levels increased two to five times for Pb, As, Zn, and Cu, about 10 to 15 times for Cr, Sb, and Hg, up to 34 times for Cd, and 25 to 67 times for DDTs, PCBs, and PAHs. The concentrations of most contaminants remained approximately constant until the late 1980s when they started to decrease slowly. The decreasing trends were most prominent for heavy metals and anthracene, less prominent for DDTs, and almost absent for PCBs and most PAHs. Discussion  Different temporal and spatial patterns for various contaminants make it possible to distinguish between anthropogenic point sources from local industry (anthracene, Cr, Cd), possible diffuse sources (most PAHs), and geological background (V, Co, Ni, and Mo). The observed recent trends in heavy metal and persistent organic pollutant contamination are generally consistent with data from other Central European rivers. The roughly balanced contamination levels in sediments from the lake and the adjacent river channel suggest that the oxbow lake deposits reflect immediate levels of the contamination bound to suspended particulate matter passing through the river. Conclusions  The investigated oxbow lake accumulated suspended sediment from Morava River, developing a thick sedimentary body. The sediments offer a good time framework to study historical contamination of the river on a decade time scale. Continuous contamination trends can be traced back to the early 1980s. The results show that stratigraphic analysis of cores has a good potential for identification of uninterrupted historical trends and unconformities, e.g., due to dredging. Recommendations and perspectives  Oxbow lakes may provide an alternative to floodplains and reservoir deposits when studying river contamination history. High sedimentation rates of up to 7.7 cm/year offer a very good stratigraphic resolution, making it possible to study contamination patterns on annual or even seasonal time scales. On the other hand, oxbow lakes may represent long-term contamination stores, which are unlikely to be redistributed by river erosion and, hence, may possess significant environmental risks for the farther future.  相似文献   

Comparison of early socialization practices used for litters of small-scale registered dog breeders and nonregistered dog breeders     

Korbelik J  Rand JS  Morton JM 《Journal of the American Veterinary Medical Association》2011,239(8):1090-1097

OBJECTIVE-To compare early socialization practices between litters of breeders registered with the Canine Control Council (CCC) and litters of nonregistered breeders advertising puppies for sale in a local newspaper. DESIGN-Retrospective cohort study. Animals-80 litters of purebred and mixed-breed dogs from registered (n = 40) and non-registered (40) breeders. PROCEDURES-Registered breeders were randomly selected from the CCC website, and nonregistered breeders were randomly selected from a weekly advertising newspaper. The litter sold most recently by each breeder was then enrolled in the study. Information pertaining to socialization practices for each litter was obtained through a questionnaire administered over the telephone. RESULTS-Registered breeders generally had more breeding bitches and had more litters than did nonregistered breeders. Litters of registered breeders were more likely to have been socialized with adult dogs, people of different appearances, and various environmental stimuli, compared with litters of nonregistered breeders. Litters from registered breeders were also much less likely to have been the result of an unplanned pregnancy. CONCLUSIONS AND CLINICAL RELEVANCE-Among those breeders represented, litters of registered breeders received more socialization experience, compared with litters of nonregistered breeders. People purchasing puppies from nonregistered breeders should focus on socializing their puppies between the time of purchase and 14 weeks of age. Additional research is required to determine whether puppies from nonregistered breeders are at increased risk of behavioral problems and are therefore more likely to be relinquished to animal shelters or euthanized, relative to puppies from registered breeders.  相似文献   

Participatory mapping of landscape values in a Pan-European perspective     

Maria Garcia-Martin  Nora Fagerholm  Claudia Bieling  Dimitris Gounaridis  Thanasis Kizos  Anu Printsmann  Matthias Müller  Juraj Lieskovský  Tobias Plieninger 《Landscape Ecology》2017,32(11):2133-2150

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Characterization of the otic bacterial microbiota in dogs with otitis externa compared to healthy individuals     

Juraj Korbelik  Ameet Singh  Joyce Rousseau  J. Scott Weese 《Veterinary dermatology》2019,30(3):228-e70

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