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Chhaya P. Sonekar Satyajit Kale Smita Bhoyar Neha Paliwal S. V. Shinde S. P. Awandkar Wiqar Khan S. P. Chaudhari N. V. Kurkure 《Tropical animal health and production》2018,50(1):91-96
Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66.24%) samples were positive by RBPT and ELISA, respectively. Brucella isolates (n = 2) were recovered from clinical samples. Both isolates demonstrated amplification for bcsp 31 and IS711 genes. On AMOS PCR, both the isolates amplified at 731 bp, i.e., belongs to B. melitensis species. The incidence of B. melitensis in a migratory flock warns the thorough testing and culling of Brucella-infected sheep from the flock on a continuous basis; otherwise, such incidence will be routine and poor farmers will be at a loss. 相似文献
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Pawar Bhausaheb Kale Prashant Bahurupe Jyoti Jadhav Ashok Kale Anil Pawar Sharad 《水稻科学》2015,22(6):283
This study was conducted to evaluate the effects of proline and glutamine on in vitro callus induction and subsequent regeneration and to develop a reproducible and highly efficient plant regeneration protocol in four rice genotypes, viz. Pawana, Jaya, Indrayani and Ambemohar. Considerable variation in response to plant growth regulators and amino acid supplements used was observed in all the four genotypes. Medium supplemented with proline and glutamine was shown to be superior to medium without proline and glutamine. The best callusing from mature embryo was observed on Murashige and Skoog(MS) medium supplemented with 2.0 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D), 500 mg/L proline and 500 mg/L glutamine. Shoot induction was higher in the callus obtained from medium supplemented with 500 mg/L proline and 500 mg/L glutamine. The highest shoot regeneration frequency(83.2%) was observed on MS medium with 2.0 mg/L benzylaminopurine, 0.5 mg/L 1-naphthaleneacetic acid, 500 mg/L proline, and 500 mg/L glutamine in the callus obtained from MS medium supplemented with 2.0 mg/L 2,4-D, 500 mg/L proline and 500 mg/L glutamine. Among the four genotypes, Pawana has the highest regeneration efficiency(83.2%), whereas the regeneration efficiency of the rest three rice genotypes was in the range of 32.0% to 72.3%. This optimized regeneration protocol can be efficiently used for Agrobacterium mediated genetic transformation in rice. 相似文献
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Vijay P. Kale Girish S. Joshi Parikshit B. Gohil Mukul R. Jain 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(3):361-366
Background: Fasting is an important preanalytical factor that may affect the interpretation of hematology and clinical biochemistry data in toxicology or pharmacology studies. Limited information is available on how the results may be affected by different durations of fasting. Objective: The purpose of this study was to assess the influence of fasting duration on clinical pathology results in male and female rats and to determine an optimum fasting time for preclinical studies. Methods: Male and female Wistar rats (10 each per group) were fasted for 0, 4, 8, 16, 24, and 48 hours. Changes in body weight and in the results of routine CBC and clinical chemistry analysis were evaluated by 1‐way ANOVA. Results: Body weight was significantly decreased by 4 hours of fasting in all rats, and hemoglobin concentration was significantly increased at 16 hours in male rats. Serum glucose and triglyceride concentrations in both sexes and cholesterol and high‐density lipoprotein‐C concentrations in female rats were also significantly decreased beginning at 16 hours. The creatinine concentration was increased in females after 16 hours of fasting. Serum alkaline phosphatase and alanine aminotransferase activities were significantly decreased after 8 hours in males and 16 hours in females. Conclusions: Fasting‐induced changes in clinical pathology results were consistent with hemoconcentration and altered nutrition and metabolic function. Most changes occurred at 16 hours, with minimal subsequent changes. Hence, a 16‐hour fasting duration may be recommended for preclinical studies involving clinical pathology measurements. 相似文献
4.
Gür Sibel Kale Mehmet Erol Nural Yapici Orhan Mamak Nuri Yavru Sibel 《Tropical animal health and production》2017,49(7):1531-1535
Tropical Animal Health and Production - Rift valley fever (RVF), a vector-borne zoonotic disease, is caused by a phlebovirus (family Bunyaviridae). The virus was initially characterized... 相似文献
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A field experiment on two wheat varieties namely NI-5642 and Kalyansona with foliar spray of three herbicides viz 2,4-D, atrataf and taphazine at three concentrations i.e. 250, 500 and 1000 ppm was conducted inrabi season of 1971. From the studies it was observed that all three herbicides at all the concentrations increased the protein content and the maximum increase was to the extent of 56.8 as percentage of control. Of the chemicals used 2,4-D at 500 ppm concentrations was effective in respect of protein content, yield and 1000 grain weight. 相似文献
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Zein is a group of alcohol-soluble corn proteins, which consists of several individual proteins. A single-step gel filtration chromatography method was developed to fractionate individual zeins from ethanol extracts of whole corn. A Superdex prep 75 column was used with different mobile phases to fractionate the zeins, which were analyzed by SDS-PAGE and UV spectrophotometry. With 70% aqueous ethanol as the mobile phase, fractions containing a mixture of alpha-zein/beta-zein and alpha-zein/delta-zein were obtained. With ammonium bicarbonate added to the 70% ethanol mobile phase, it was possible to obtain beta-zein and delta-zein fractions devoid of other proteins. However, all fractions containing alpha-zein also contained minor amounts of delta-zein and/or beta-zein. Almost all fractions also contained non-protein impurities. 相似文献
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The degradation of14 C-Carbofuran was studied in sterilized, unsterilized and green manure amended clay soil under moist and flooded conditions overa period of 30 days. The14 C mass balance showed that carbofuran did not undergo any degradation in sterilized moist soil. In sterilized flooded soil bound residues were formed to the extent of about 47% of the applied radioactivity at the end of 30 days. Carbofuran underwent considerable degradation in unsterilized moist and flooded soils. In moist soil about 48% of the applied14 C activity was recovered as bound activity while in flooded soil, about 23% of the activity was bound. Green manure amendment resulted in formation of more bound residues under moist conditions while it enhanced the degradation of carbofuran under flooded conditions. In flooded amended soil about 44% of the appliedl4 C-activity was recovered as against about 54% in the unamended flooded soil. The notable degradation products formed under flooded soil conditions were 3-keto carbofuran and 3-hydroxy carbofuran. 相似文献
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Crop productivity and nutrient use are mainly water restricted in semi-arid regions. This study was conducted to find out whether the onion seed crop productivity could be elevated through drip fertigation. The effects of irrigation and fertigation levels on yield, yield components, quality, and nutrient use of onion seed crop (Allium cepa L.) were investigated at Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra, western India on a sandy clay loam soil. Irrigation water applied at evapotranspiration (ETc) levels at 80% (I1), 90% (I2), and 100% (I3), whereas drip fertigation levels at 60%, 80%, 100%, and 120% of recommended dose [120:60:60 nitrogen, phosphorus, potassium (NPK) kg/ha] were investigated. Three-year experiment results showed no significant differences in number of seed per umbel, seed yield per umbel per plant, seed and straw yield per hectare from the comparison between 100% ETc and 90% ETc. In fertigation, 120% and 100% levels gave significantly higher values of yield components and seed yield than the 80% and 60% levels. The quality parameters decreased with decrease in ETc, but increased with increase in fertigation levels. Fertilizer-use efficiency (FUE) was highest in 60% fertigation and then declined with the increase in fertigation levels. Irrigation at 100% ETc and fertigation at 120% resulted in higher nutrient use, but the difference with 90% ETc and 100% fertigation was non-significant. The 90% ETc and 100% fertigation dose (120:60:60 NPK kg/ha) appears a useful practice to increase onion seed productivity under the semi-arid climate of western India. 相似文献
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Kale M Yavru S Ata A Kocamüftüoglu M Yaplcl O Haslrcloglu S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):331-336
In this study, blood serum and leukocyte samples were collected from 400 Holstein heifers, all of which appeared to be healthy. Antibodies (Ab) against bovine viral diarrhea virus (BVDV) were detected in 57 serum samples, and BVDV antigen (Ag) was detected in 38 leukocyte samples. There were statistically important differences between the average first insemination ages (FIT) of the BVDV (Ag-/Ab+) heifers (p<0.0001) (pregnant p<0.05, nonpregnant p<0.0001) and BVDV (Ag-/Ab-) heifers. The average conception rates (CR) of BVDV (Ag-/Ab+) heifers and BVDV (Ag-/Ab-) heifers were not significant statistically. There were statistically important differences in average FIT between persistent infected (PI) BVDV (Ag+/Ab-) heifers (p<0.0001; PI pregnant p<0.05, PI nonpregnant p<0.0001) and BVDV (Ag-/Ab-) heifers. No significant differences in average CR between PI BVDV (Ag+/Ab-) heifers and BVDV (Ag-/Ab-) heifers were found. The differences in average FIT between BVDV (Ag+/Ab+; p<0.0001; nonpregnant p<0.0001) and BVDV (Ag-/Ab-) heifers were important statistically. Although there were no BVDV (Ag+/Ab+) pregnant heifers, the differences in average CR between BVDV (Ag+/Ab+) pregnant heifers and BVDV (Ag-/Ab-) heifers were found to be statistically important (p<0.0001). We conclude that fertility is affected in heifers with BVDV (Ag-/Ab+, Ag+/Ab- and Ag+/Ab+). 相似文献