Nutrition and feeding of dogs and cats. Past, present and future     

F A Kallfelz 《The Cornell veterinarian》1985,75(1):221-229

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Death of a neonatal lamb due to Clostridium perfringens type B in New Zealand     

JS Munday  H Bentall  D Aberdein  M Navarro  FA Uzal  S Brown 《New Zealand veterinary journal》2020,68(4):242-246

ABSTRACT

Case history and clinical findings: A flock of 20 sheep was kept within three paddocks on a single property. None of the animals in the flock had been vaccinated against any disease for at least three years. Abdominal bloating and haemorrhagic diarrhoea were observed in Lamb 1 at 24 hours-of-age. The lamb subsequently died within an hour of the onset of clinical signs. Lamb 2 was 3-days-old when observed to be recumbent with opisthotonus. The lamb was treated with dextrose, vitamins B1 and B12, and penicillin G, but died 4 hours later.

Pathological findings: Examination of Lamb 1 revealed markedly increased gas within the peritoneum and within dilated loops of intestine. The intestines were dark red and contained large quantities of haemorrhagic fluid. Histology of the intestines revealed peracute mucosal necrosis with minimal accompanying inflammation. The intestinal lumen contained cell debris, haemorrhage, and myriad large Gram-positive bacilli. The intestines of Lamb 2 did not appear bloated or reddened. However, multiple fibrin clots were visible within the pericardial sac. Histopathological examination revealed small foci of necrosis within the mucosa of the distal intestine. The necrotic foci were often associated with large numbers of large Gram-positive bacilli.

Immunohistochemsitry and molecular biology: Intestinal samples from Lamb 1 were processed for Clostridium perfringens immunohistochemistry, which revealed large numbers of intralesional, positively immunostained rods. Fragments corresponding to the expected sizes for genes encoding alpha, beta, and epsilon C. perfringens typing toxins were amplified by PCR from DNA extracted from formalin-fixed sections of intestine.

Diagnosis: Lamb dysentery due to C. perfringens type B.

Clinical relevance: C. perfringens bacteria have a worldwide distribution, but disease due to C. perfringens type B has only been diagnosed in a small number of countries and has never been reported in New Zealand or Australia. C. perfringens type B produce both beta toxin and epsilon toxins, therefore both haemorrhagic enteritis and systemic vascular damage can develop. As many animals are exposed to C. perfringens without developing disease, there must be additional unknown factors that resulted in disease in these particular sheep. Vaccines that specifically protect against C. perfringens type B are available and may be recommended for use in smaller non-commercial flocks, as in the present case.  相似文献   

An experimental model of chronic renal disease in dogs by infusion of microspheres into the renal arterial circulation     

D A Dzanis  L Krook  H J Harvey  F A Kallfelz 《American journal of veterinary research》1990,51(4):682-687

The feasibility of renal arterial infusion of nonbiodegradable microspheres as a model of chronic renal disease in dogs was evaluated. Resin-coated, styrene-divinyl benzene copolymer microspheres were infused into the kidneys of healthy adult Beagles by direct injections of both renal arteries in a single surgical procedure. Injections of 25-microns diameter microspheres had minimal effect on either the clinical status or serum values of the dogs. Histologic examination revealed the majority of the microspheres lodged within the capillary beds of the glomeruli, and little change to the kidneys. However, injections of 50-microns diameter microspheres caused significant increases in serum concentrations of urea nitrogen and creatinine. Histologically, the larger microspheres obstructed afferent arterioles and small arteries, which caused diffuse glomerular necrosis and nephron damage. With doses ranging from 1 to 3 million microspheres/dog, a correlation between the quantity of microspheres injected and severity of renal damage was observed. The optimal dose for producing a model of moderate renal disease was determined to be 1.8 million microspheres/dog (0.9 million microspheres/kidney). During long-term studies, microsphere-injected dogs fed a moderately restricted protein ration remained relatively azotemic, compared with control dogs on the identical ration. During the 5-month postsurgical period, the serum urea nitrogen concentration averaged 18.41 +/- 1.59 mg/dl (mean +/- SE) for the microsphere-injected dogs vs 9.31 +/- 0.38 for the control dogs (P less than 0.001). Similarly, the mean serum creatinine value was significantly higher (P = 0.020) for the microsphere-injected dogs, compared with the controls (1.23 +/- 0.12 mg/dl vs 0.94 +/- 0.03).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

国有苗圃所有制结构调整之我见     

周国平  李发凯 《江西林业科技》2001,(Z1)

所有制结构的调整是国有苗圃发展壮大的出路所在.笔者从必要性,战略方向,基本途径等三个方面阐述了看法.  相似文献   

3种阔叶树育苗技术及苗木生长规律研究初探   总被引：2，自引：0，他引：2  

刘志强  肖水清  邹元熹  肖才发 《江西林业科技》2001,(3):14-15

通过对闽楠，蓝果树，东京野茉莉的育苗试验，研究苗木生长规律，掌握生理生物学特性。  相似文献   

Determination of total serum thyroxine in the dog by competitive protein binding of labeled thyroxine     

F A Kallfelz 《American journal of veterinary research》1969,30(6):929-932

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Analysis of embedded gas detection system based on nano-porphyrin arrays     

LUO Xiao gang  BAI Xing hong  HOU Chang jun  HUO Dan qun  FA Huan bao  YANG Mei 《保鲜与加工》2011,(12):48-53

In view of the necessity of gas detection in environment, an embedded gas detection system is designed based on the basic principles of chromatography change of chemical reaction between nano-porphyrin arrays and micro-gas, which takes ARM9 S3C2440A as the core and nano-porphyrin array as the sensors. The overall program, the circuit organization and control software of the main function module such as spectrum signal acquisition, processing and monitoring are presented. With energy conservation differential equation, the control model of temperature and flow rate in the sample chamber is established, which can precisely control the detection conditions of chemical sensors. Some typical trace gases such as ammonia, enanthaldehyde, cyclohexane and hexanal are tested by the system, and the results show that the system can achieve rapid and accurate detection of trace volatile gas.  相似文献   

阔叶红松林山杨伐根分解过程中真菌动态研究     

法蕾  赵秀海 《北京林业大学学报》2006,(Z2)

山杨是长白山阔叶红松林中的常见阔叶树种,择伐后的伐根很多.山杨伐根在多种真菌的作用下逐渐分解,不断地向周围释放养分,构成保证森林生态系统稳定循环的一个重要环节.随分解的不断进行,山杨伐根上的真菌的种类和数量都呈现出一定的变化趋势.真菌数量高峰出现在伐后的第5年,而种类高峰则出现在伐后的第7年.这一时间上的交错现象是由真菌的不同分解能力引起的.分解能力强的主要分解菌将难于分解的木质素、纤维素等分解为易于利用的小分子物质,为其他真菌提供了营养来源,便于其他真菌的定殖.另外,伐根上的真菌数量还与伐根桩径呈正相关关系.  相似文献   

Thyroid function in the dog.     

F A Kallfelz 《The Veterinary clinics of North America》1977,7(3):497-512

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Scintigraphic diagnosis of primary hepatocellular carcinoma in the woodchuck (Marmota monax)     

F A Kallfelz  W E Hornbuckle  H J Harvey  R J Wallace  L G Potkewitz  L Roth  B C Tennant 《American journal of veterinary research》1986,47(3):573-576

Hepatic imaging with 99mTc-sulfur colloid was used to diagnose primary hepatocellular carcinoma (PHC) in woodchucks infected with woodchuck hepatitis virus (WHV). Based on imaging results, 6 of 12 WHV-infected woodchucks had space-occupying hepatic lesions, and all 6 had PHC. Of the remaining woodchucks, 2 did not have PHC, 2 had discrete tumors (less than 1 cm diameter), 1 had miliary small tumors, and 1 had tumors located near the great vessels. Hepatic imaging was a valuable technique for diagnosis of PHC in WHV-infected woodchucks.  相似文献