The value of sea urchin,Lytechinus variegatus,egesta consumed by shrimp,Litopenaeus vannamei     

Karen E. Jensen  J. Christopher Taylor  Robert J. Barry  Louis R. D'Abramo  D. Allen Davis  Stephen A. Watts 《Journal of the World Aquaculture Society》2019,50(3):614-621

Sea urchins produce high‐energy, membrane‐bound fecal pellets that contain residual nutrients and large quantities of microbiota. These egesta are readily consumed by the shrimp, Litopenaeus vannamei. Egesta of the sea urchin, Lytechinus variegatus, were evaluated as a feed supplement or total replacement for a commercial shrimp diet. Shrimp were stocked at 0.49 g ± 0.06 g initial body weight and housed individually in 2.8‐L tanks in a commercial recirculating zebrafish system. Shrimp were assigned to one of six diets: commercial shrimp feed, reference sea urchin feed, collected dried sea urchin egesta, collected wet sea urchin egesta, half ration of shrimp feed and half collected wet sea urchin egesta, and egesta naturally produced by two sea urchins in polyculture. Equivalent dry matter amounts of each diet were proffered to shrimp in each treatment twice daily, except for those that had complete access to natural egesta excreted by sea urchins in polyculture. Sea urchins were proffered a reference sea urchin feed at 2% body weight daily. After 27 days, shrimp proffered collected dried or wet egesta did not differ significantly in percent weight gain and showed the lowest weight gain. The percent weight gain of shrimp fed the commercial shrimp diet did not differ significantly from that of the shrimp fed half commercial shrimp diet and half egesta. The highest weight gain was recorded for those shrimp that consumed the untouched egesta produced by sea urchins in polyculture. These data suggest that consumed egesta have noteworthy nutritional value and therefore would be beneficial to the culture of extractive species in an integrated multitrophic aquaculture system.  相似文献   

Clinical response of nasal adenocarcinoma to cisplatin chemotherapy in 11 dogs.     

K A Hahn  D W Knapp  R C Richardson  C L Matlock 《Journal of the American Veterinary Medical Association》1992,200(3):355-357

In 11 dogs with nasal adenocarcinoma, IV treatment with 2 to 8 cycles of cisplatin at a dosage of 60 mg/m2 of body surface, given at 3-week intervals, resulted in a survival time of 8 to 128 weeks (mean, 32.7 weeks; median, 20 weeks). Radiography revealed complete remission in 2 dogs and partial remission in 1 dog, for an overall response rate of 27%. Clinical problems such as nasal discharge (n = 11), sneezing (n = 6), and epistaxis (n = 4) resolved in 1 to 2 weeks in all dogs after beginning chemotherapy.  相似文献   

Observations on transplacental infection with bluetongue virus in sheep   总被引：1，自引：0，他引：1  

C Richardson  W P Taylor  S Terlecki  E P Gibbs 《American journal of veterinary research》1985,46(9):1912-1922

Twenty-four ewes were inoculated with 1 of 2 strains of bluetongue virus type 4 at 40, 60, or 80 days of gestation. Two ewes aborted, 2 ewes died, and 1 was killed during the experiment, but their fetuses were recovered. At term, 2 mummified fetuses, 4 dead lambs, and 17 clinically healthy lambs were produced by 12 sheep, and the remaining 7 sheep were barren. Porencephaly and cerebellar dysgenesis were found in term lambs born to sheep inoculated at 40 and 60 days of gestation. Radiographic examination of 12 fetuses showed developmental ages far less than their chronologic age; 8 fetuses had skeletal growth-retardation lines, which were also observed in the dead lambs. A systemic lymphoreticular hyperplasia was observed in the dead lambs and in all lambs at 12 weeks of age; in 4 of the latter, granulomatous reactions were present in the liver and kidney. Lungs of the full-term lambs were reduced in weight and showed poor alveolar development and mononuclear cell infiltration, which persisted in the 12-week-old lambs. It was concluded that bluetongue virus is capable of causing not only gross abnormalities of the CNS, but also generalized growth retardation and fetal lymphoreticular hyperplasia.  相似文献   

COMPUTED TOMOGRAPHY-GUIDED PERCUTANEOUS BIOPSY: CRITERIA FOR ACCURATE NEEDLE TIP IDENTIFICATION     

Amy S.  Tidwell  DVM  Karen L.  Johnson  BS  CNMT 《Veterinary radiology & ultrasound》1994,35(6):440-444

Precise localization of the needle tip during CT-guided percutaneous biopsy is considered to be a key element of a successful procedure. To ensure accuracy, the true needle tip must be differentiated from a false or simulated tip which appears when the CT slice encompasses only the shaft of an angled needle. By obtaining images of an aspiration biopsy needle inserted vertically into a phantom and then incrementally tilting the gantry, the authors were able to compare the characteristic features of the true tip to the simulated tip. The true tip was abrupt and distinct and had an adjacent flame-like low density artifact. The simulated tip was indistinct and tapered, yet still produced the adjacent artifact. We concluded that the shape and distinctness of the end portion of the needle itself, rather than the attendant artifact, were the most reliable criteria for accurate needle tip identification.  相似文献   

Experimental Yersinia enterocolitica placentitis in sheep.     

M J Corbel  B Ellis  C Richardson  R Bradley 《The British veterinary journal》1992,148(4):339-349

A strain of Yersinia enterocolitica of O serogroup 6,30 isolated from the liver of an aborted ovine fetus was inoculated intravenously into a group of pregnant ewes at about 90 days gestation and produced placentitis with abortion or delivery of infected lambs about 50 days later. Y. enterocolitica of the same serogroup was recovered from the necrotic placental cotyledons and most other fetal tissues and could be isolated from vaginal discharges of the ewes for a least 2 weeks after abortion. Histological changes were consistent with an acute bacterial necrotizing placentitis and systemic infection of the fetus. Subsequent pregnancies in the ewes proceeded to term without evidence of infection.  相似文献   

Neurological Manifestations of Niemann-Pick Disease Type C in Cats   总被引：3，自引：1，他引：2  

Karen R. Muñana  Patricia J. Luttgen  Mary Anna Thrall  Thomas W. Mitchell  David A. Wenger 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(2):117-121

Seven Domestic shorthair cats with a lysosomal storage disorder analogous to human Niemann-Pick disease type C, from a breeding colony were studied to characterize the neurological manifestations of this disorder. Affected cats were identified by means of liver biopsies at 4 to 6 weeks of age. Neurological examinations were performed at 2 week intervals from the onset of clinical signs. All cats displayed signs referrable to the cerebellum, with a subtle intention tremor noticed initially at 8 to 12 weeks of age; the disease was rapidly progressive. The tremor became more pronounced, menace response was lost, and severe dysmetria and ataxia developed. Three cats also had signs referrable to other areas of the central nervous system. Cats died or were euthanized between 12 and 43 weeks of age. Pathological findings included accumulation of substrate within neurons throughout the central nervous system, and axonal spheroid formation. The clinical and pathological findings in these cats are comparable to those in the human form of the disease.  相似文献   

0–7–21 Radiation Therapy for the Treatment of Canine Oral Melanoma     

Karen E. Bateman DVM  MSc    Pamela A. Catton  Paul W. Pennock  Stephen A. Kruth 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(4):267-272

Eighteen dogs with malignant melanoma of the oral cavity were treated with high-dose per fraction (0–7–21) radiation therapy. Eight hundred cGy was administered on days 0, 7, and 21 for a total dose of 2,400 cGy in 3 weeks. Of 17 dogs evaluated, 9 (53%) had a complete remission and 5 (30%) achieved a partial remission with an overall response rate of 83%. Local failure occurred in 2 of the 9 dogs where a complete response was initially observed. One dog died of intercurrent disease, and one died of metastatic disease without evidence of local recurrence. Five dogs are alive and free of disease 9 to nineteen months from the initiation of therapy. The 0–7–21 protocol was well-tolerated, and acute radiation reactions were low-grade and limited to the skin. The results of this study demonstrate that oral melanomas in dogs are responsive to radiation. 0–7–21 radiation therapy offers a viable alternative to radical excision, especially when tumor volume or location would require cosmetically or functionally debilitating surgery.  相似文献   

Transforming growth factor type beta (TGF-beta) and adipogenesis in pigs   总被引：1，自引：0，他引：1  

R L Richardson  D R Campion  G J Hausman  J T Wright 《Journal of animal science》1989,67(8):2171-2180

The present study was performed on s.c. adipose tissue of fetal pigs at 35 to 110 d of gestation to examine the distribution of TGF-beta-positive cells, to localize TGF-beta immunoreactivity at the cellular level using electron microscopy (EM), and to determine the effect of TGF-beta on primary cultures of pig adipose tissue cells. Tissues for EM were fixed and embedded in LR white resin. Sections then were incubated with a polyclonal antibody specific for TGF-beta and TGF-beta was located using 20 nm colloidal gold conjugated second antibody. Tissues were fixed and embedded in paraffin for localization of TGF-beta at the light microscope (LM) level. Tissues were incubated with anti-TGF-beta followed by localization using biotinylated second antibody. Using LM, only a few cells stained positively for TGF-beta within developing blood vessels at 35 d. By 50 d, more TGF-beta-positive cells were associated with forming capillary networks. Between 70 d and 110 d, positively stained adipocytes usually were clustered around blood vessels. Cells surrounding hair follicles stained positive for TGF-beta between 90 to 110 d. Electron microscopy revealed TGF-beta labeling within fat cells. Fibroblasts and endothelial cells did not exhibit TGF-beta immunoreactivity. The addition of TGF-beta to primary cultures of s.c. adipose tissue cells from newborn pigs prevented lipid filling in fat cells. This effect was dose-dependent, with half-maximal inhibition occurring at 3 pM maximum inhibition occurred at 40 pM. These results indicate that TGF-beta may regulate angiogenic activity and lipid filling in s.c. adipose tissue of fetal pigs. Although TGF-beta was present in adipocytes and in cells associated with developing capillary networks, the physiological role of TGF-beta during early adipose tissue development is not known.  相似文献   

Subchondral lucency of the third carpal bone in Standardbred racehorses: 13 cases (1982-1988)     

M W Ross  D W Richardson  G A Beroza 《Journal of the American Veterinary Medical Association》1989,195(6):789-794

Thirteen Standardbreds had subchondral lucency of the third carpal bone (C3), described as single or multiple central areas of C3 bone loss in the radial fossa. Sclerosis of the radial fossa was also detected. The mean age of 9 stallions, 3 mares, and 1 gelding was 4.1 years (range, 3 to 7 years). All horses had an acute moderate to severe lameness referable to the middle carpal joint. A dorsoproximal dorsodistal (skyline) radiographic projection was most useful and identified mild (3 horses), moderate (6 horses), and severe (4 horses) subchondral lucency and sclerosis of the radial fossa. The margin of C3 was intact dorsal to the lucent areas in all horses. In 12 horses, arthroscopic surgical evaluation and curettage of the lesion were performed. Of the 9 horses monitored long enough to allow racing, 8 horses (89%) returned to racing, although only 6 (75%) raced at the same degree of competition. Subchondral lucency of C3 resulted from chronic damage to C3 with subsequent osteochondral collapse or acute C3 proximal surface osteochondral fracture.  相似文献   

Soil mixing in the East Anglian fens     

S.J. Richardson  C.J. Dyer  S.N. Jewell 《Soil Use and Management》1991,7(1):30-33

Abstract. Experiments were started in 1967 to investigate the effects of soil mixing on fen peat soil. Peaty topsoil was mixed with mineral subsoil to a maximum depth of 80 cm at two sites, one with a clay subsoil, the other sand. Mixing was done on a commercial scale with a range of implements. After initial increases, yields of arable crops on both mixed soils were subsequently similar to those on unmixed soil. Residual herbicide activity was enhanced on mixed soils, and evidence of peat conservation was obtained at one site. The practice has not been taken up commercially because of the high capital cost of mixing and lack of serious problems which had been predicted following peat wastage.  相似文献